• The Bulletin of The Korean Astronomical Society
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• v.40 no.2
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• pp.54.1-54.1
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• 2015

We observed 38 Planck Cold Clumps (PCCs) in the $850{\mu}m$ dust continuum emission using the JCMT/SCUBA-2, and detected the emission in 15 clumps containing dense cores. In this poster we present the preliminary results. The PCCs are cold, dense, and thus, they are considered as objects in the early evolutionary stages of star formation. The sources in our sample were selected based on the Purple Mountain Observatory (PMO) 13CO (1-0) integrated intensity maps. In order to examine whether these cores detected in $850{\mu}m$ continuum have potential to be prestellar cores, we compare each core mass estimated from the $850{\mu}m$ continuum with the Virial mass and Bonnor-Ebert (BE) mass calculated from the 13CO (1-0) or C18O (1-0) spectra. By comparing the two column densities from the dust continuum and the 13CO (1-0) or C18O (1-0) line, we also derive the CO depletion factor, which could be an indicator of core evolution. The moment maps of the 13CO (1-0) line are used to study the physical properties (e.g. kinematics, turbulence) of PCCs. We investigate difference between the sources with and without detectable $850{\mu}m$ emission to study the formation conditions of dense cores.

• Mass Spectrometry Letters
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• v.4 no.2
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• pp.25-29
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• 2013

Proteomics for biomarker validation needs high throughput instrumentation to analyze huge set of clinical samples for quantitative and reproducible analysis at a minimum time without manual experimental errors. Sample preparation, a vital step in proteomics plays a major role in identification and quantification of proteins from biological samples. Tryptic digestion a major check point in sample preparation for mass spectrometry based proteomics needs to be more accurate with rapid processing time. The present study focuses on establishing a high throughput automated online system for proteolytic digestion and desalting of proteins from biological samples quantitatively and qualitatively in a reproducible manner. The present study compares online protein digestion and desalting of BSA with conventional off-line (in-solution) method and validated for real time sample for reproducibility. Proteins were identified using SEQUEST data base search engine and the data were quantified using IDEALQ software. The present study shows that the online system capable of handling high throughput samples in 96 well formats carries out protein digestion and peptide desalting efficiently in a reproducible and quantitative manner. Label free quantification showed clear increase of peptide quantities with increase in concentration with much linearity compared to off line method. Hence we would like to suggest that inclusion of this online system in proteomic pipeline will be effective in quantification of proteins in comparative proteomics were the quantification is really very crucial.

• Journal of Biomedical Engineering Research
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• v.25 no.1
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• pp.49-55
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• 2004

In this paper, we investigated the effects of the photodynamic therapy(PDT) for the tumor mass in mice. In the experimental method, we divided the mice into two control and test group which HepG2 and HeLa cell line induced cancer mass in mice. Photofrin was administered to the tumor-bearing mouse, followed 30 hours later by 630nm and 650nm laser light exposure. After photodynamic therapy we analyzed the two mice group for the tumor mass size, tumor growth, tumor cell necrosis, pathological anatomy change. According to the results, tumor cell necrosis was shown in the tissues which the reduce size of tumor and tumor cell necrotic change according to the irradiation time and light dose amount. The considerable difference, however, between the 630nm and 650nm wavelength was not found for the tumor cell necrotic change and other damage of normal tissue was not found.

• Journal of The Korean Astronomical Society
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• v.39 no.1
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• pp.9-17
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• 2006

In an attempt to investigate star formation activity and statistical properties of clumps of high Galactic latitude clouds (HLCs), we mapped the Polaris Flare region, PF121.3+25.5, in $^{12}CO\;and\;^{13}CO$ J = 1 - 0 using SRAO 6-m telescope and also observed its 12 $^{13}CO$ peak positions in CS J = 2 - 1 with TRAO 14-m telescope. $^{13}CO$ integrated intensity map shows clearly its clumpy structure and the locations of clumps well agree with $^{12}CO$morphology. CS line is not detected toward the 12 $^{13}CO$ peak positions, so we can conclude there are no dense $(\sim10^4\;cm^{-3})$ in this region. We decomposed 105 clumps from $^{13}CO$ map using GAUSSCLUMPS algorithm. The mass of clumps ranges from $7.8\;M_{\odot}\;to\;7.4{\times}10^{-2}\;M_{\odot}$ with a total mass of $66.4\;M_{\odot}$ The mass spectrum follows a power law, dN/dM ${\propto}\;M^{-\alpha}$ with a power index of ${\alpha}=1.91{\pm}0.13$. The virial masses of clumps are in the range of $10{\sim}100M_{LTE}$ and so these clumps are considered to be gravitationally unbound.

• The Bulletin of The Korean Astronomical Society
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• v.44 no.1
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• pp.69.1-69.1
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• 2019

We present the ALMA observations of the infrared dark cloud (IRDC) core MSXDC G053.11+00.05 MM1 at the distance of 1.7 kpc. While the core was first identified at 1.2 mm with a mass of 124 Msun, recent near- and mid-infrared observations have revealed a parsec-scale molecular hydrogen (H2 1-0 S(1) at 2.12 micron) outflow and two early class young stellar objects (YSOs) at the center of the core, one of which is likely massive (M > 8 Msun). From the ALMA Band 7 observations with a resolution of 0.5", we have found a dust filament of < 0.1 pc in which five dense cores are embedded in the 870 micron continuum. The brightest core is consistent with one of the two previously-detected YSOs, but the other four are newly discovered implying their very deeply embedded status. We have also detected several molecular line emission including H13CO+ and C17O as well as 13CO outflow with complicated morphology. At the brightest core, the methanol line (CH3OH) shows velocity gradients, which may support the existence of a circumstellar disk around a high-mass protostar. Based on the derived properties of the dense cores, we discuss their association with the two YSOs and H2 outflow detected in infrared and high-mass star-formation process occurring in IRDC cores.

• Tunnel and Underground Space
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• v.6 no.3
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• pp.197-208
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• 1996

Due to the constraints in pre site-investigation for tunnel, it is essential to redesign the support structures suitable for rock mass conditions such as rock strength, ground water and discontinuity conditions for safe tunnel construction. For the selection of optimum support, it is very important to carry out the rock mass classification and in-situ measurement in tunnelling. In this paper, in a mountain tunnel designed by NATM in hard rock, the selectable system for optimum support has been studied. The tunnel is situated at Chun-an in Kyungbu highspeed railway line with 2 lanes over a length of 4, 020 m and a diameter of 15 m. The tunnel was constructed by drill & blasting method and long bench cut method, designed five types of standard support patterns according to rock mass conditions. In this tunnel, face mapping based on image processing of tunnel face and rock mass classification by RMR carried out for the quantitative evaluation of the characteristics of rock mass and compared with rock mass classes in design. Also, in-situ measurement of convergence and crown settlement conducted about 30 m interval, assessed the stability of tunnel from the analysis of monitoring data. Through the results of rock mass classification and in-situ measurement in several sections, the design of supports were modified for the safe and economic tunnelling.

• Mass Spectrometry Letters
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• v.4 no.3
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• pp.41-46
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• 2013

The goal of this study is to find an experimental condition which enables us to perform enzymatic studies on the cellular behavior of PTEN (phosphatase and tensine homolog) through identification of molecular species of phosphatidylinositol 3,4,5-trisphosphates and their quantitative analysis in a mammalian cell line using mass spectrometry. We initially exployed a two-step extraction process using HCl for extraction of phosphatidylinositol 3,4,5-trisphosphates from two mammalian cell lines and further analyzed the extracted phosphatidylinositol 3,4,5-trisphosphates using tandem mass spectrometry for the identification of them. We finally quantified the concentration of phosphatidylinositol 3,4,5-trisphosphates using internal standard calibration. From these observation, we found that HEK 293-T cells is a good model to examine the enzymatic behavior of PTEN in a cell, and the minimum amount of phosphatidylinositol 3,4,5-trisphosphates is more than 50 pmol for quantification in a mass spectrometer. These results suggest that the well-optimized experimental conditions are required for the investigation of the cellular PTEN in terms of the catalytic mechanism and further for the detailed identification of cellular substrates.

• Journal of The Korean Astronomical Society
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• v.38 no.2
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• pp.279-282
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• 2005

We report the millimeter-wave radio observations of molecular cloud cores in Taurus. The observed line is the $N_2H^+$ emission at 93 GHz, which is known to be less affected by molecular depletion. We have compared starless (IRAS-less) cores with star-forming cores. We found that there is no large difference between starless and star-forming cores, in core radius, linewidth, core mass, and radial intensity profile. Our result is in contrast with the result obtained by using a popular molecular line, in which starless cores are larger and less condensed. We suggest that different results mainly come from whether the employed molecular line is affected by depletion or not. We made a virial analysis, and found that both starless and star-forming cores are not far from the critical equilibrium state, in Taurus. Together with the fact that Taurus cores are almost thermally supported, we conclude that starless Taurus cores evolve to star formation without dissipating turbulence. The critical equilibrium state in the virial analysis corresponds to the critical Bonnor-Ebert sphere in the Bonnor-Ebert analysis (Nakano 1998). It is suggested that the initial condition of the molecular cloud cores/globules for star formation is close to the critical equilibrium state/critical Bonnor-Ebert sphere, in the low-mass star forming region.

• Journal of the Korean Society of Industry Convergence
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• v.20 no.5
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• pp.431-439
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• 2017

Since the power transmission line(PTL) passes through the high mountain and heavy snowfall region, it is necessary to keep the stability of the PTL. In this study, PTL is modeled as a mass-spring-damper system by using RecurDyn. The lumped mass model is verified by calculated from the simulation comparing the deflection analysis according to the sag and tension. In order to analyze the dynamic behavior of PTL, a damping coefficient for a multi-body model is derived by using the free vibration test and Rayleigh damping theory. Sleet jump simulation according to the region is performed. The maximum jump height, icing sag and amount of jump are confirmed. Also, the amount of jump and the reaction force at the supporting point according to the tension and load of ice are analyzed, respectively. As a result, it is noted that the amount of jump and reaction force are influenced more by the load of ice than by the tension of PTL.

• Publications of The Korean Astronomical Society
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• v.15 no.spc1
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• pp.133-145
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• 2000

Symbiotic stars are known as binary systems with both cool and hot components with enshrounding nebulous gas. The cool component, M-type giant, is presumably loosing its mass into a hot white or main sequence companion star through the inner Lagrangian point. The lines emit from the ionized nebulous region around the hot star while the mass loss or accretion activity is believed to be the main cause of sudden variation of the continuum and line fluxes. We selected 17 symbiotics for which the emission line fluxes were measured from the IUE SWP, LWR data, to find variability of spectrum. We also investigated the periodic variation of emissions or eclipsing effect from the IUE lines. All of our symbiotics show very high electron densities in the emission regions. For other optical symbiotics, the observations had been carried in 1999 with BOAO mid-resolution spectrometer. We classified symbiotics based on their outburst activities, or emission line characteristics, i.e., $OVI{\lambda}6830.\;The\;OVI{\lambda}6830$ emission lines are also found in S-type symbiotics, which have been known as charateristics of D-types.