• 보존과학연구
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• 통권22호
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• pp.27-40
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• 2001

DNA typing is often used to determine identity from human remains. Recently, the molecular biological analysis of ancient deposits has become possible since methods for the recovery of DNA conserved in bones or teeth from archaeological remains have been developed. In the field of archaeology, one of the most promising approaches is to identify the individuals present in a mass burial site. We performed nuclear DNA typing and mitochondrial DNA sequencing analysis based on PCR from a Korea ancient human remain excavated from Sa-chon Nuk-island and civilian access controlline(CACL). A femur bone were collected and successfully subjected to DNA extraction, quantification, PCR amplification, and subsequently typed for several shot tandem repeat(STR)loci. 4 types of STR systems used in this study were CTT multiplex(CSF1PO, TPOX, TH01), FFv multiplex(F13A01, FESFPS, vWA), Silver STRⅢ multiplex(D16S539, D7S820, D13S317), and amelogenin for sex determination. This studies are primarily concerned with the extraction, amplification, and DNA typing of ancient human bone DNA samples. Also, it is suggestive of importance about closely relationship between both fields of archaeology and molecular biology.

• 한국IT서비스학회지
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• 제14권1호
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• pp.263-276
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• 2015

As a social being, people can cooperate and control one another through the power of reputation, which is a critical opinion of someone given by others. Nevertheless, there have been obstacles in clarifying the identity of traditional types of reputation, for they are mostly words of mouth passed among members of a society. However, due to dramatic technological advancement and widespread use of the Internet and social media, now we can clearly see and analyze written reputations, which used to be passed only from mouth to mouth. Against this background, this study examines whether a negativity bias-a notion that an event of a more negative nature has a greater effect on one's psychological state than a positive event-applies to spread of reputation online, and examines related factors and effects. To this end, reputation-related online comments left by social media users during the election period of Korea's 6th provincial election on 4 June 2014 were analyzed. For the analysis, a Bass diffusion model was used, which is based on the innovation diffusion theory. The analysis results confirmed that, at online forum, negative reputations spread more quickly and more widely than positive ones, had a greater impact, and mass media such as online news outlets had a significant influence on spread of reputation online.

• Asian-Australasian Journal of Animal Sciences
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• 제27권4호
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• pp.561-566
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• 2014

The complete coding sequence of Wild Argali ISG15 cDNA was generated by rapid amplification of cDNA ends. The ISG15 cDNA was 642 bp with an open reading frame of 474 bp, which encoded a 17.47 kDa protein composed of 157 amino acids. Its amino acid sequence shared 97.9%, 80.8%, 91.4%, 94.3%, 78.3% identity with those of ISG15cDNA from Ovis aries (accession no. NM001009735.1), Capra hircus (accession no. HQ329186.1), Bos taurus (accession no. BC102318.1), Bubalus bubalis (accession no. HM543269.1), and Sus scrofa (accession no. EU647216.1), respectively. The entire coding sequence was inserted into the pET-28a vector and expressed in E. coli. The recombinant protein corresponded to the expected molecular mass of 25 kDa as judged by SDS-PAGE, and it was detected in the bacterial inclusion bodies. The expressed protein could be purified by $Ni^{2+}$ chelate affinity chromatography and the results from the lymphocyte proliferation test showed that the product could stimulate lymphocyte proliferation very well (p<0.05), which further confirmed its biological activity.

• Bulletin of the Korean Chemical Society
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• 제31권2호
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• pp.275-280
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• 2010

Actinobacillus actinomycetemcomitans is a gram-negative, nonmotile coccobacillus bacterium that is associated with several human diseases, including endocarditis, meningitis, osteomyelitis, subcutaneous abscesses and periodontal diseases. A full-length Omp1-like protein gene from A. actinomycetemcomitans was cloned into a pQE30 vector and overexpressed in Escherichia coli BL21(DE3) cells. The protein revealed sequence homologies to Seventeen kilodalton proteins (Skp) from Pasteurella multocida and E. coli that have been characterized as periplasmic chaperones. This soluble Omp1-like protein was successfully purified to homogeneity for further folding and functional studies. The purity, identity, and conformation of the protein were determined using sodium dodecyl sulfate polyacrylamide gel electrophoresis, matrix-assisted laser desorption ionization mass spectrometry, circular dichroism, fluorescence spectroscopic, and differential scanning calorimetric studies. We showed that the protein formed an oligomer larger than a tetramer. We found, further, that it is comprised of mostly $\alpha$-helices and boasts high thermal stability.

• 한국디지털정책학회:학술대회논문집
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• 한국디지털정책학회 2005년도 춘계학술대회
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• pp.505-516
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• 2005

According to Mead's (1934) symbolic interaction theory, social roles are learned through interaction and experiences in everyday life. Over time, these rules of behavior become internalized and serve to structure one's actions accordingly. Ultimately, these rules provide us with a powerful means of controlling our actions, and in time, they define our identity. Transforming the socialization process is one's conscious interpretation of stimuli through the use of symbols. Furthermore, society's perceptual processes can be shaped by the symbols we learn. The meaning of symbols can be learned from a variety of social influences, one of which may be mediated messages and advertising. This paper attempts to establish a link between media exposure and one's perception of social reality regarding character judgments made of unknown others based on the target's product or brand usage. Using magazine advertisements for fictitious products, the experiment herein seeks to establish two fundamental goals: 1) to determine if perceptions can be manipulated via association with companion symbolic elements: and 2) to detect whether television exposure is a moderating factor. Respondents were asked their perceptions of both product quality and of product users.

• 한국CDE학회논문집
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• 제16권4호
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• pp.305-313
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• 2011

This paper proposes a method for reusing kinematic design data for virtual facilities, Making a virtual model of a facility involves two major activities: geometric design (virtual model visualization) and kinematic design that should be remodeled frequently whenever design changes occur, Conventionally, a virtual model of an automated facility focuses on the design level, which mainly deals with design verification, alternative comparison, and geometric model diagnosis, Although a design level model can be designed with the information of past models from PLM system, a simulation level model is not sufficient utilized to be reused for kinematic design purpose, We propose a method for reusing kinematic information of a past simulation model to cope with this problem, We use the concept or the 'center of mass', which is a point representing the mean position of the matter in a body or system. And we also use comparison method of a boundary box to identity which 3D objects have to be involved from the design model to a link structure that is contained in the simulation model. Because a proposed method only use not a historical approach but a geometrical approach, it is more effective to apply to the field.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.402-406
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• 2005

Lipomyces starkeyi produces a novel glucanhydrolase containing endo-dextranase and ${\alpha}-amylase$ activities. A cDNA from L. starkeyi encoding a dextranase was isolated and characterized. The 2,052 kb cDNA fragment (lsd1) carrying dextranase gene showed one open reading frame (ORF) composed of 1,824 bp flanked by a 41 bp 5'-UTR and a 184 bp 3'-UTR including a poly(A) tail of 27 bp. The ORF encodes for a 608 amino acid with a predicted molecular mass of 67.6 kDa. There was 77% deduced amino acid sequence identity between the LSD1 dextranase and the dextranase from Penicillium minioluteum. The primary structure of the dextranase from L. starkeyi has distant similarity with enzymes belonging to glycosyl hydrolase family 49. The lsd1 protein was expressed in the Saccharmyces cerevisiae under control of GAL1 promoter and active dextranase was produced.

• 한국가구학회지
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• 제10권1호
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• pp.33-42
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• 1999

This study aims to organize the research ways of interior design for clothing stores as a management strategy in the market changing rapidly. The public met their needs in a mass production system. However, the brand-name companies began to create the conditions of consumption for people, there appeared people with new lifestyles and new way of thoughts so that their propensity to consume resulted in consumption activities, and it become varied, individual, and highly qualified. Therefore, in view of consumers' priority, based on SI sales environment this paper aims to create a new image by re-designing the interior and furniture of shops which will help to enhance the image of brand name. Therefore, the brand-name companies will expect a large sale for the new product and it will apply to sales environments of other products and influence them. The purpose of this paper is to find a desirable way to increase sales efficiency for the companies. In order to get a concept and propriety of sales environment design, examined mutual relation to each study of interior design, marketing, and fashion design. Through referring to the documents research of these fields I could get a conception and basic structure for them. Based on the theoretical knowledge, this study was made to show to examine and generalize the existing sales environments and situations and set up a new concept for this. It will apply to interior design and store holds and present a new design alternative for improvement.

• 디자인학연구
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• 제17권2호
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• pp.67-76
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• 2004

소비의 주체성이 생산과 구조적 측면보다 쾌적함, 온유함, 유머 등의 디자인 제2차 적 가치의 현상으로 전의 되고 있으며 이와 같은 잉여성과 관련된 개념들은 디자인에서뿐만 아니라 최근 과학에서도 공간과 물질, 동일성과 중력 둥과 같은 개념이 사라지고 불확정성, 개연성, 모순, 애매모호함, 엔트로피 등과 같은 개념들로 대체되고 있다. 따라서 디자인과 관련된 인접학문의 지적동향과 주된 관심사의 탐구과정을 통하여 아이디어 전개에 필요한 그림과 이미지들의 변형과 조작 등 디자인의 제2차 적 가치와 잉여적 가치에 관련이 깊고 실제 아이디어 전개의 확장성과 다의성에 관련된 개념과 변형과 조작의 제4열 사고생성체계를 제시하고 자 한다.

• 사상체질의학회지
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• 제22권3호
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• pp.132-140
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• 2010

1. Objective: To compare the contents and transfer rate of hazardous substances in crude, washing solution, crude after washing, decoction and remnant after boiling. 2. Methods: The heavy metal contents of each step were measured by inductively coupled plasma mass spectrometer (ICP-MS) and mercury analyzer (MA-2). In order to analyze pesticides in each sample we used simultaneous multi-residue analysis of pesticides by GC/ECD, which was followed by GC/MSD analysis to confirm the identity of the detected pesticide in each sample. In addition, the contents of sulfur dioxide (SO2) were performed by Monier-Williams distillation method. 3. Results: 1) Contents (mg/kg) of heavy metals in decoction of all herbal medicine prescriptions were not detected. 2) Transfer rates (%) of heavy metals from crude to remnant were As (83.3%), Cd (100.0%), Pb (182.6%) and Hg (100.0%). 3) Contents (mg/kg) of residual pesticides were not detected. 4) Transfer rate (%) of sulfur dioxide (SO2) from crude to remnant was 44.2%. 4. Conclusion: Our results showed that boiled herbal medicine prescriptions which we take is safe from the hazardous substances.