• Title/Summary/Keyword: Marker-variable Technique

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Analyzing Common Method Bias of the Korean Empirical Studies on Technology Acceptance Model (한국 TAM 실증연구의 동일방법편의 분석)

  • Baek, Sang-Yong
    • The Journal of Information Systems
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    • v.21 no.1
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    • pp.1-17
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    • 2012
  • Common Method Bias(CMB) may cause the potential inflation of correlations between measures assessed via the same method. The problem of CMB has been well known in behavioral sciences because the survey method with self-reporting is vulnerable to CMB. Thus, the discussion on CMB is still ongoing in the MIS research in US. However, in Korea, the MIS research has never paid attention on the CMB problem. The purpose of this study is to examine the CMB problem in the Korean MIS research. To evaluate the effect of CMB, empirical studies on Technology Acceptance Model(TAM) are selected because (1) TAM is one of the MIS research areas studied intensively, (2) TAM is a theoretical model well supported by the existing empirical studies so that the result of this study would have a great ripple effect when the CMB problem turned out to be serious, (3) CMB is domain-specific. 47 TAM samples (out of 45 studies) from three Korean Journals were selected and the relevant data were collected such as correlation matrixes and the measures of the dependent variable. To find and evaluate the size of CMB, two analytic methods (Marker-Variable Technique and Method-Method Pair Technique) are employed. The result showed that there exists CMB in the Korean studies but the problem is not so serious to distort the empirical testing, compared with that of US studies. However, considering that CMB can contaminate the testing results, Korean MIS researchers should explicitly deal with the problem in designing empirical studies and collecting data.

An Improvement of Still Image Quality Based on Error Resilient Entropy Coding for Random Error over Wireless Communications (무선 통신상 임의 에러에 대한 에러내성 엔트로피 부호화에 기반한 정지영상의 화질 개선)

  • Kim Jeong-Sig;Lee Keun-Young
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.43 no.3 s.309
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    • pp.9-16
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    • 2006
  • Many image and video compression algorithms work by splitting the image into blocks and producing variable-length code bits for each block data. If variable-length code data are transmitted consecutively over error-prone channel without any error protection technique, the receiving decoder cannot decode the stream properly. So the standard image and video compression algorithms insert some redundant information into the stream to provide some protection against channel errors. One of redundancies is resynchronization marker, which enables the decoder to restart the decoding process from a known state in the event of transmission errors, but its usage should be restricted not to consume bandwidth too much. The Error Resilient Entropy Code(EREC) is well blown method which can regain synchronization without any redundant information. It can work with the overall prefix codes, which many image compression methods use. This paper proposes EREREC method to improve FEREC(Fast Error-Resilient Entropy Coding). It first calculates initial searching position according to bit lengths of consecutive blocks. Second, initial offset is decided using statistical distribution of long and short blocks, and initial offset can be adjusted to insure all offset sequence values can be used. The proposed EREREC algorithm can speed up the construction of FEREC slots, and can improve the compressed image quality in the event of transmission errors. The simulation result shows that the quality of transmitted image is enhanced about $0.3{\sim}3.5dB$ compared with the existing FEREC when random channel error happens.

Phylogenetic Relationships and Genetic Diversity in Collected Resources of Carthamus tinctorius by Random Amplified Polymorphic DNA Markers (RAPD 마커에 의한 수집된 홍화자원에서 계통관계와 유전적 다양성)

  • Sung, Jung-Sook;Cho, Gyu-Taek;Lee, Gi-An;Baek, Hyung-Jin;Huh, Man-Kyu
    • Journal of Life Science
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    • v.20 no.12
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    • pp.1764-1771
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    • 2010
  • Carthamus tinctorius L. (Compositae) is an herb primarily distributed throughout in the world. The species is regarded as ecologically important in the world. Safflower was used for medicines, as well as making red (carthamin) and yellow dyes. We have used the RAPD technique to investigate the phylogenetic relationships and genetic diversity of C. tinctorius. We obtained 123 bands from all the 26 cultivars. The average number of bands was 9.5 per primer. The genetic diversity of safflower is found among cultivars and there is a high among-cultivar differentiation. The OPC18-01 band is the specific marker for Syria cultivar, whereas no products were detected in individuals from other country cultivars. We found seven phenetic bands for determining the specific marker of cultivars with SCAR markers. Though the number of individuals sampled for analysis was small and probably not fully representative of the total available diversity in C. tinctorius, this study demonstrates that the regions (Morocco, Syria, and Turkey) of the Mediterranean Sea were more variable than other regions with the exception of India. In this result, although only simple result of RAPD is difficult to assert the center of species diversity of C. tinctorius, the regions of the Mediterranean Sea may be the most probable candidate for the origin of safflower. India was also the candidate of the center or secondary center of species diversity of C. tinctorius. RAPD markers were effective in classifying cultivar levels of safflower.

RAPD Analysis of Three Deer Species in Malaysia

  • El-Jaafari, Habiba A.A.;Panandam, Jothi M.;Idris, Ismail;Siraj, Siti Shapor
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.9
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    • pp.1233-1237
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    • 2008
  • The genetic variability within and among three deer species in Malaysia, namely Cervus nippon (sika), Cervus timorensis (rusa) and Cervus unicolor (sambar), were evaluated using the RAPD technique. The DNA extracted from the buffy coat of 34 sika, 38 rusa and 9 sambar were analysed using ten primers that gave bands which showed good resolution. The primers generated 164 RAPD markers in total, and these ranged in size from 150 to 900 bp. The percent of polymorphism of the bands generated per primer ranged from 66.66-93.33% for rusa, 36.84-61.14% for sambar and 52.38-100% for sika. The overall percent polymorphism observed for the 164 RAPD markers was 99.39%. The results revealed five exclusive, monomorphic markers for sambar and one exclusive, monomorphic marker for sika; none was observed for rusa. However, these cannot be declared as markers for the identification of the species without analysis of more samples, populations and species. The means of within population genetic distances, based on Dice's and Jaccard's similarity indices, were similar for the rusa (0.383 and 0.542, respectively) and sika (0.397 and 0.558, respectively) populations with the sambar population being the least variable (0.194 and 0.323, respectively). The Dice based genetic distances within the species ranged from 0.194 to 0.397 and the genetic distances among the species were 0.791-0.911. The genetic distances based on Dice's and Jaccard's similarity indices between the rusa and sambar were 0.556 and 0.713, between the rusa and sika populations were 0.552 and 0.710, and between sambar and sika were 0.622 and 0.766, respectively.

A fast and reliable polymerase chain reaction method based on short interspersed nuclear elements detection for the discrimination of buffalo, cattle, goat, and sheep species in dairy products

  • Cosenza, Gianfranco;Iannaccone, Marco;Gallo, Daniela;Pauciullo, Alfredo
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.6
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    • pp.891-895
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    • 2019
  • Objective: Aim of present study was the set up of a fast and reliable protocol using species-specific markers for the quali-quantitative analysis of DNA and the detection of ruminant biological components in dairy products. For this purpose, the promoter of the gene coding for the ${\alpha}$-lactoalbumin (LALBA) was chosen as possible candidate for the presence of short interspersed nuclear elements (SINEs). Methods: DNA was isolated from somatic cells of 120 individual milk samples of cattle (30), Mediterranean river buffalo (30), goat (30), and sheep (30) and the gene promoter region (about 600/700 bp) of LALBA (from about 600 bp upstream of exon 1) has been sequenced. For the development of a single polymerase chain reaction (PCR) protocol that allows the simultaneous identification of DNA from the four species of ruminants, the following internal primers pair were used: 5'-CACTGATCTTAAAGCTCAGGTT-3' (forward) and 5'-TCAGA GTAGGCCACAGAAG-3' (reverse). Results: Sequencing results of LALBA gene promoter region confirmed the presence of SINEs as monomorphic "within" and variable in size "among" the selected species. Amplicon lengths were 582 bp in cattle, 592 bp in buffalo, 655 in goat and 729 bp in sheep. PCR specificity was demonstrated by the detection of trace amounts of species-specific DNA from mixed sources ($0.25ng/{\mu}L$). Conclusion: We developed a rapid PCR protocol for the quali-quantitative analysis of DNA and the traceability of dairy products using a species-specific marker with only one pair of primers. Our results validate the proposed technique as a suitable tool for a simple and inexpensive (economic) detection of animal origin components in foodstuffs.

Effects of Anti-thyroglobulin Antibody on the Measurement of Thyroglobulin : Differences Between Immunoradiometric Assay Kits Available (면역방사계수법을 이용한 Thyroglobulin 측정시 항 Thyroglobulin 항체의 존재가 미치는 영향: Thyroglobulin 측정 키트에 따른 차이)

  • Ahn, Byeong-Cheol;Seo, Ji-Hyeong;Bae, Jin-Ho;Jeong, Shin-Young;Yoo, Jeong-Soo;Jung, Jin-Hyang;Park, Ho-Yong;Kim, Jung-Guk;Ha, Sung-Woo;Sohn, Jin-Ho;Lee, In-Kyu;Lee, Jae-Tae;Kim, Bo-Wan
    • The Korean Journal of Nuclear Medicine
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    • v.39 no.4
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    • pp.252-256
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    • 2005
  • Purpose: Thyroglobulin (Tg) is a valuable and sensitive tool as a marker for diagnosis and follow-up for several thyroid disorders, especially, in the follow-up of patients with differentiated thyroid cancer (DTC). Often, clinical decisions rely entirely on the serum Tg concentration. But the Tg assay is one of the most challenging laboratory measurements to perform accurately owing to antithyroglobulin antibody (Anti-Tg). In this study, we have compared the degree of Anti-Tg effects on the measurement of Tg between availale Tg measuring kits. Materials and Methods: Measurement of Tg levels for standard Tg solution was performed with two different kits commercially available (A/B kits) using immunoradiometric assay technique either with absence or presence of three different concentrations of Anti-Tg. Measurement of Tg for patient's serum was also performed with the same kits. Patient's serum samples were prepared with mixtures of a serum containing high Tg levels and a serum containg high Anti-Tg concentrations. Results: In the measurements of standard Tg solution, presence of Anti-Tg resulted in falsely lower Tg level by both A and B kits. Degree of Tg underestimation by h kit was more prominent than B kit. The degree of underestimation by B kit was trivial therefore clinically insignificant, but statistically significant. Addition of Anti-Tg to patient serum resulted in falsely lower Tg levels with only A kit. Conclusion: Tg level could be underestimated in the presence of anti-Tg. Anti-Tg effect on Tg measurement was variable according to assay kit used. Therefore, accuracy test must be performed for individual Tg-assay kit.