• 대한의생명과학회지
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• 제29권4호
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• pp.302-313
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• 2023

Y chromosomal short tandem repeat (Y-STR) markers have been developed continuously to complement forensic DNA analyses and population genetic studies. Initially, we collected data from previously reported Korean population Y-STR haplotype studies on 1133 individuals. We then conducted a marker expansion analysis using a dataset from the Y-STR Haplotype Reference Database (YHRD), covering up to 29 Y-STRs, referred to as Ymax. Additionally, we examined the impact of rapidly mutating (RM) Y-STRs included in this expanded marker set on the discrimination capacity. We observed that marker expansions both with (0.9896), and without (0.9510), RM Y-STR improved the discrimination capacity. Subsequently, we focused on 16 individuals belonging to seven distinct groups sharing identical haplotypes. These particular haplotypes had been previously identified among 476 unrelated males using 23 Y-STR markers from the PowerPlex^® Y23 System. We expanded the marker panel up to Ymax to explore how discrimination improved with an expansion of Y-STR markers for these 16 individuals. Among the expanded markers, DYS627, which had high discriminatory power, had a high mutation rate (1.10 × 10^-2) and high gene diversity (0.83). In contrast, DYF387S1 displayed high gene diversity (0.95) but a relatively low mutation rate (2.80 × 10^-3). We propose that these findings will be valuable in the selection of suitable Y-STR markers, depending on the objectives of forensic analyses. Additionally, the presence of frequently observed Y-haplotypes in Korean population will facilitate statistical interpretation in Y-STR DNA profiling.

• 한국축산식품학회지
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• 제31권4호
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• pp.530-536
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• 2011

It is well established that uncoupling protein 3 (UCP3) is expressed largely in skeletal muscle, white adipose tissue and brown adipose tissue and has been suggested to play important roles in regulating energy expenditure, body weight, thermoregulation as well as fatty acid metabolism and obesity. Therefore, the UCP3 gene was selected as a candidate gene for carcass and meat quality traits in Korean cattle. The objective of this study was to identify single nucleotide polymorphisms (SNPs) in the UCP3 gene and to evaluate the association of UCP3 SNP markers with carcass and meat quality traits in Korean cattle. The five exons in the UCP3 gene were sequenced, and ten SNPs were identified. The PCR-SSCP method was then developed to genotype the individuals examined. The g.3076A>G genotype was significantly associated with marbling score (MS) of Korean cattle. Animals with the AA genotype had a higher MS than those with the AG and GG genotypes. No significant associations of the SNP g.3076A>G were observed for any traits. In conclusion, although SNP g.3076A>G, which showed an association with MS, does not cause amino acid changes, this SNP may be used as a DNA marker to select animals that have higher intramuscular fat content.

• Asian-Australasian Journal of Animal Sciences
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• 제14권4호
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• pp.587-596
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• 2001

In the last decade, rapid developments in molecular biotechnology and of genomic tools have enabled the creation of dense linkage maps across whole genomes of human, plant and animals. Successful development and implementation of interval mapping methodologies have allowed detection of the quantitative trait loci (QTL) responsible for economically important traits in experimental and commercial livestock populations. The candidate gene approach can be used in any general population with the availability of a large resource of candidate genes from the human or rodent genomes using comparative maps, and the validated candidate genes can be directly applied to commercial breeds. For the QTL detected from primary genome scans, two incipient fine mapping approaches are applied by generating new recombinants over several generations or utilizing historical recombinants with identity-by-descent (IBD) and linkage disequilibrium (LD) mapping. The high resolution definition of QTL position from fine mapping will allow the more efficient implementation of breeding programs such as marker-assisted selection (MAS) or marker-assisted introgression (MAI), and will provide a route toward cloning the QTL.

• Applied Biological Chemistry
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• 제36권6호
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• pp.557-561
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• 1993

Polyethylene glycol(PEG)법 또는 electroporation법으로 제라니움 원형질체에 neomycin phosphotransferase II(nptII) 유전자를 옮기고, 세포내에 도입된 nptII DNA의 존재유무와 발현여부를 조사하였다. Polymerase chain reaction(PCR)을 이용하여 검토한 결과, PEG법을 사용했을 때나 electroporation법을 사용했을 때 모두 세포내에 도입된 nptII DNA가 있음이 확인되었다. 또 이들 세포의 추출물을 전기영동하여 neomycin phosphotransferase 활성을 조사한 결과, 효소활성을 보이는 band가 검출되어 marker gene 으로 도입된 notII 유전자가 세포내에서 발현된다는 것이 확인되었다.

• 한국작물학회지
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• 제46권5호
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• pp.416-419
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• 2001

Hypernodulation soybean mutant, SS2-2, is characterized with greater nodulation and nitrogen fixing ability in the root nodule than its wild type, Shinpaldalkong 2. The present study was performed to identify a genetic locus conferring hypernodulation in soybean mutant SS2-2 and to determine whether the gene controlling the hypernodulation of SS2-2 is allelic to that controlling the supernodulation of nts382 mutant. Hybridization studies between SS2-2 and Taekwangkong revealed that the recessive gene was responsible for the hypernodulation character in soybean mutant SS2-2. Allelism was also tested by crossing supernodulating mutant nts382 and hypernodulating mutant SS2-2 that both hypernodulation and supernodulation genes were likely controlled by an identical locus. Molecular marker mapping of hypernodulation gene in SS2-2 using SSR markers confirmed that the gene conferring hypernodulation was located at the same loci with the gene conferring supernodulation. It is interesting to note that the same gene controlled the super- and hyper-nodulation characters, although SS2-2 and nts 382 exhibited differences in the amount of nodulation in the root system. Further genetic studies should be needed to clarify the genetic regulation of super- and hyper-nodulation in soybean.

• 한국컴퓨터정보학회논문지
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• 제15권12호
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• pp.197-207
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• 2010

대용량(High-throughput) 형태로 얻어진 cDNA 마이크로어레이 데이터에 다양한 데이터 마이닝 기법을 적용하면 서로 다른 조직에서 추출한 유전자의 발현정도를 비교할 수 있고 정상세포와 암세포에서 발현량의 차이를 보이는 DEG(Differently Expression Gene) 유전자를 추출할 수 있다. 이들을 이용하여 병을 진단할 수 있을 뿐만 아니라, 암의 진행 단계(Cancer Stage)에 따른 치료 방법을 결정할 수 있다. 마이크로어레이를 기반으로 한 대부분의 암 분류자는 기계학습 기법을 이용하여 암 관련 유전자를 추출하여, 이들 유전자를 총체적으로 이용하여 독립 샘플의 클래스(암, 정상)를 판정한다. 하지만 유전자의 발현량의 차이뿐만 아니라 유전자와 유전자의 상관관계의 변화가 질병 진단에 활용될 수 있다. 대부분의 질병은 단독 유전자의 변이에 의한 것이 아니라 유전자의 모듈로 이루어진 유전자조절네트워크의 변이에 의한 것이기 때문이다. 본 논문에서는 조건에 따라 특이적 관계를 나타내는 유전자 쌍을 식별하여, 이들 유전자 쌍을 이용한 유전자 분류 모듈을 생성한다. 분류 모듈을 이용한 암 분류 방법이 기존의 암 분류 방법보다 높은 정확도로 암과정상 샘플을 분류함을 보여주고 있다. 분류 모듈을 구성하는 유전자의 수가 상대적으로 적으므로 임상키트로의 개발도 고려할 수 있다. 향후 분류 모듈에 속하는 유전자의 기능적 검증을, GO(Gene Ontology)를 활용함으로서, 밝혀지지 않은 새로운 암 관련 유전자를 식별하고, 분류 모듈을 확대하여 암 특이적 유전자조절네트워크 구성에 활용할 계획이다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권3호
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• pp.217-222
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• 2004

As Agrobacterium tumefaciens, which has long been used to transform plants, is known to transfer T-DNA to budding yeast, Saccharomyces cerevisiae, a variety of fungi were subjected to the A. tumefaciens-mediated transformation to improve their transformation frequency and feasibility. The A. tumefaciens-mediated transformation of chestnut blight fungus, Cryphonectria parasitica, is performed in this study as the first example of transformation of a hardwood fungal pathogen. The transfer of the binary vector pBIN9-Hg, containing the bacterial hygromycin B phosphotransferase gene under the control of the Aspergillus nidulans trpC promoter and terminator, as a selectable marker, led to the selection of more than 1,000 stable, hygromycin B-resistant transformants per 1${\times}$10$\^$6/ conidia of C. parasitica. The putative transformants appeared to be mitotically stable. The transformation efficiency appears to depend on the bacterial strain, age of the bacteria cell culture and ratio of fungal spores to bacterial cells. PCR and Southern blot analysis indicated that the marker gene was inserted at different chromosomal sites. Moreover, three transformants out of ten showed more than two hybridizing bands, suggesting more than two copies of the inserted marker gene are not uncommon.

• 한국수산과학회지
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• 제40권3호
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• pp.128-132
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• 2007

We identified a microsatellite marker, Poli121TUF, which appears to be significantly linked (P<0.001) with a lymphocystis disease virus (LCDV)-resistance gene in the olive flounder, Paralichthys olivaceus. The olive flounder is an economically important food fish, that is widely cultured in Korea, Japan, and China. Lymphocystis disease has spread in these countries and has seriously reduced the economic value of the fish. LCDV causes lymphocystis cells (LC) to form on the body surface, fins, gills, mouth, and intestine. Fish with LC lose commercial value due to their deformed appearance. The identified micro satellite marker can be used as a candidate locus for marker-assisted selection (MAS) in order to enhance the efficiency of selection for LCDV resistance in the olive flounder.

• 생명과학회지
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• 제12권6호
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• pp.715-720
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• 2002

Arabidopsis thaliana columbia의 종자에 EMS를 처리하여 돌연변이체들을 만들었고 이중 UV-B에 감수성이 높은 돌연변이체를 골랐다. 이 UV-B 감수성 돌연변이체의 원인 유전자를 밝히기 위하여 교배 실험을 한 결과 이는 Mendel 유전법칙을 따르고 단일 유전자의 돌연변이에 의하여 나타나며 열성 유전을 하는 것으로 밝혀져 이 유전자를 uvs라 하였다. 염색체상의 uvs의 위치를 밝히기 위하여 CAPS maker를 이용한 연관분석을 하고자 하였고 이를 위하여 각각 maker의 primer 10종류를 제작하였다. 이를 이용, 각 PCR 산물에 대하여 uvs mutant와는 다른 제한효소 pattern를 갖는 Lansberg와 uvs mutant를 교배시켜서 얻은 것들로부터 DNA를 추출하여 PCR을 수행하였다. 이들과 자외선과의 감수성을 연관시켜 교차율을 계산한 결과 5번 염색체의 LFY3과 가장 가까웁게 연관되어 있었다.

• Animal Systematics, Evolution and Diversity
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• 제36권4호
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• pp.347-353
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• 2020

Although sea slaters Ligia have a significant role in rocky shore habitats, their taxonomic entities have not been clearly understood. In this study, we investigated whether genetic variation inferred from a nuclear genetic marker, namely amplified fragment length polymorphism (AFLP), would conform to that of a mitochondrial DNA marker. Using both the mitochondrial DNA marker and the AFLP marker amplified by the six selective primer sets, we analyzed 95 Ligia individuals from eight locations from East Asia. The direct sequencing of mitochondrial 16S rRNA gene revealed three distinct genetic lineages, with 9.8-11.7 Kimura 2-parameter genetic distance. However, the results of AFLP genotyping analysis with 691 loci did not support those of mitochondrial DNA, and revealed an unexpectedly high proportion of shared polymorphisms among lineages. The inconsistency between the two different genetic markers may be explained by difference in DNA evolutionary history, for example inheritance patterns, effective population size, and mutation rate. The other factor is a possible genomic island of speciation, in that most of the genomic parts are shared among lineages, and only a few genomic regions have diverged.