• Journal of Plant Biotechnology
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• v.2 no.3
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• pp.135-142
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• 2000

The activity of promoter sequences was evaluated in garlic cells using the $\beta$-glucuronidase (GUS) gene as a reporter. Histochemical GUS assay indicated transient GUS activity in leaf, callus and root cells 48 hours after particle bombardment transformation. Quantitative fluorometric assays in extracts of transformed leaves demonstrated that the CsVMV promoter induced the highest level of gene expression, which was, on average, ten fold the level induced by CaMV35S and by the Arabidopsis Act2 promoters and two fold the level expression observed with a construct containing a double CaMV35S plus the untranslated leader sequence from AMV. No activity or very low levels were observed when cells were transformed with plasmids rontaining the typical monocot promoters, Actl, from rice or the Ubi-1, from maize. The green fluorescent protein (GFP) was also tested as a marker gene for garlic transformation. Intense fluorescence was observed in leaf, callus and root cells transformed with a construct containing the gfp gene under control of the CaMV35 Promoter. No fluorescence was detected when the gfp was under control of the Ubi-1 promoter.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.9 no.4
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• pp.958-963
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• 2008

Korean language has different structural properties which are controlled by semantic constraints of verbs. Also, most of Korean sentences are complex sentences which consisted of main clause and embedded clause. Therefore it is difficult to describe appropriate syntactic grammar or constraint for the Korean language and the Korean parsing causes various syntactic ambiguities. In this paper, we suggest how to describe CFG-based grammar using sentence patterns as syntactic constraint and solve syntactic ambiguities. To solve this, we classified 44 sentence patterns including complex sentences which have subordinate clause in Korean sentences and used it to reduce syntactic ambiguity. However, it is difficult to solve every syntactic ambiguity using the information of sentence patterns. So, we used semantic markers with semantic constraint. Semantic markers can be used to solve ambiguity by auxiliary particle or comitative case particle.

• Toxicological Research
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• v.17 no.4
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• pp.255-265
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• 2001

Epidemiologic studies have demonstrated an association between short-term exposure to particulate air pollutants and increased mortality. However the biological mechanism underlying these associations have not been fully established and also the chemical and physical characteristics of the pollutant particles are not well understood. The metal constituents of air pollutant particles and their bioavailability are considered to Play an important role as possible mediators of Particle-induced airway injury and inflammation. Sprague-Dawley rat alveolar macrophage cells (NR8383) were exposed to airborne and acid-leached particulate matter (PM). Titanium oxide and nickel subsulfide were used as negative and positive controls. Particle-induced reactive oxygen species formation in cells was detected using the fluorescent probe 2',7'-dichlorofluorescin diacetate. Expression of TNF-$\alpha$ and IL-6 were measured by enzyme-linked immunosorbent assay, and PM-induced DNA double-strand breaks were determined with $\lambda$DNA/Hind III marker. Metals associated with air pollutant particles mediated intracellular oxidant production in alveolar macrophages, and the cytotoxicity and proinflammatory cytokine production induced by PM were associated with oxidative stress. The oxidants produced by air pollutant particles also are likely to induce DNA double-strand breaks. Our findings in alveolar macrophage cells exposed to PM and acid-leached PM support the hypothesis that metal components in urban air pollutants and their bioavailabilities might play an Important role in the induction of the adverse health effects.

• Applied Biological Chemistry
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• v.39 no.4
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• pp.260-264
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• 1996

Process of particle bombardment for efficient transformation of Cymbidium virescence rhizome microcross sections was investigated using Biolistic particle delivery system with pBI121 harboring the ${\beta}-glucuronidase$(GUS) and the neomycin phosphotransferaseII(nptII). The best result was obtained from the combination of $1.11{\;}{\mu}m$ tungsten particles coated with pBl121, $77.33kg/cm^2$ helium pressure, 6.35 mm gap distance, and 7.0 cm target distance. Transient expression of the reporter gene, GUS, bombarded into the rhizome microsections was observed by the histochemical assay. The marker gene, nptII, delivered by bombarding the tungsten particles coated with the plasmid DNA was identified in the transformed rhizome by polymerase chain reaction.

• Smart Structures and Systems
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• v.24 no.6
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• pp.793-802
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• 2019

The centrifuge model test is usually used for two-dimensional deformation and instability study of the soil slopes. As a typical loose slope, the municipal solid waste (MSW) landfill is easy to slide with large deformation, under high water levels or large earthquakes. A series of centrifuge model tests of landfill slide induced by rising water level and earthquake were carried out. The particle image velocimetry (PIV), laser displacement transducer (LDT) and marker tracer (MT) methods were used to measure the deformation of the landfill under different centrifugal accelerations, water levels and earthquake magnitudes. The PIV method realized the observation of continuous deformation of the landfill model, and its results were consistent with those by LDT, which had higher precision than the MT method. The deformation of the landfill was mainly vertically downward and increased linearly with the rising centrifugal acceleration. When the water level rose, the horizontal deformation of the landfill developed gradually due to the seepage, and a global slide surface formed when the critical water level was reached. The seismic deformation of the landfill was mainly vertical at a low water level, but significant horizontal deformation occurred under a high water level. The results of the tests and analyses verified the applicability of PIV in the two-dimensional deformation measurement in the centrifuge model tests of the MSW landfill, and provide an important basis for revealing the instability mechanism of landfills under extreme hydraulic and seismic conditions.

• Journal of Ocean Engineering and Technology
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• v.11 no.2
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• pp.70-76
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• 1997

수치격자의 크기제한에 의한 자유표면 유동해석 문제를 효율적으로 다루기 위하여 자유표면의 모든 격자를 x 방향으로 4,8,12개로 등분할 하고, y 방향으로는 4개로 잘라서 계산하였다. 이중격자 또는 삼중격자로 Navier-Stokes 방정식의 각항에 크기가 다른 격자를 사용해 효율을 향상시키는 계산방법의 연장으로, 본 논문에서는 자유표면 방정식에 보다 세분화된 격자를 적용해, Marker Particle 이동 및 자유수면 형성에 효율향상을 줄수 있는 수치방법을 도입하였다. 계산결과에 의하면 초기사용 격자가 coarse한 경우가 본방법의 효과가 커짐을 알 수 있고 대상물로는 층류유동에서 Wigley모형과 낭류유동의 S103 모형이다.

• Language and Information
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• v.19 no.2
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• pp.37-53
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• 2015

It has been widely accepted that Korean -i/ka, known as the nominative marker, can give rise to exhaustivity effect. However, it has been rarely discussed and still remains unclear how and why the particle can convey exhaustivity. The purpose of this paper is to shed light on this issue, based on Kim's (2014) analysis of the function of -i/ka. The main claim is that the exhaustivity expressed by -i/ka is a pragmatic effect derived from the interaction of the function of -i/ka, namely, unique specification, and the existence of alternatives to the denotation of an -i/ka-marked phrase in discourse context. Thus, the nature of the exhaustive implicature induced by -i/ka is not conventional but conversational.

• Language and Information
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• v.6 no.2
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• pp.15-32
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• 2002

These analyses can be roughly classified into two groups: the first argue that the delimiter -to is polysemous so that it delivers either additive or concessive meaning; the second contend that it is monosemous and only conveys emphatic or concessive meaning. The current analysis mainly focuses on its two roles with regard to focus and its meaning. On the basis of the findings, a different analysis is proposed that it serves as an indicator of the presence of a type of focus which is hosted mainly by the expression it attaches to. Furthermore, it is solely responsible for the additive force, and the seemingly relevant emphatic or concessive import is derivable from an emphatic or concessive illocutionary operator which is associated with the focus indicated by -to.

• Journal of Plant Biotechnology
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• v.41 no.4
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• pp.216-222
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• 2014

This study was carried out to develop an efficient transformation protocol via particle bombardment with PLBs (protocorm-like bodies) in Phalaenopsis. To achieve this aim, osmoticum treatment and an increasing shooting chances in particle bombardment process were applied for this study. In addition, pCAMBIA3301: ORE7 vector which contains a herbicide-resistance bar gene as a selectable marker and ORE7 gene as a gene of interests were employed. With regard to the increasing chances of shooting in particle bombardment, double shooting was the best results with 1.5 ~ 2.5 times higher than those of a single or triple shooting treatment in the productioon of PPT (D-L-phosphinothricin)-resistant PLBs. However, regeneration rate of shoots in double shooting was not high as a single shooting. Further, double shooting showed 35 ~ 40% higher than that of a single shooting in the frequency of browning. Regarding effects of different osmotic treatments, combination of 0.2 M sorbitol with 0.2 M mannitol showed the best results in transformation efficiency, regeneration of transformants and reduction of browning. Putative transgenic Phalaenopsis plants were analyzed by PCR analysis and confirmed the presence of bar and ORE 7 gene. Also, real-time PCR was conducted by using 21 transgenic plants and showed only 4 plants had one copy of transgene; whereas, the other 17 plants had more than 2 copies of transgene. Transgenic phalaenopsis plants produced in this study were transferred to pots and flowered normally without morphological variations in flower and leaf.

• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.82-88
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• 2017

Transgenic lily plants have been obtained after particle bombardment, using PDS-1000/He system and scale explants of lilies, followed by PPT (D-L-phosphinothricin) selection. In this study, scales of the lily plants cv. 'red flame' were bombarded with a plasmid containing the bar gene as a selectable marker, and the AtSIZ gene as a gene of interest, showing salt tolerance and drought tolerance respectively, and both being driven by the CaMV 35S promoter. For optimization of a protocol, factors which optimized and showed a high transformation efficiency under following conditions, were considered: a bombardment pressure of 1100 psi, a target distance of 6 cm and $1.0{\mu}m$ of gold particle, and 24-h pre-culture and post-culture on MS medium containing 0.2 M sorbitol and 0.2 M mannitol as osmoticum agents. After bombardment, all the bombarded scales of lily were transferred to MS medium without selective agents, for a week. Subsequently, these bombarded scales were transferred to a selection MS medium containing 10 mg/l PPT, and incubated for a month for further selection, after which they were cultured for another 4-8 weeks with a 4-week subculture regime on the same selection medium. After transferring into hormone-free MS medium, the PPT-resistant scales with shoots were successfully rooted and regenerated into plantlets. PCR analysis revealed that the surviving putatively transformed plantlets indicated the presence of both the bar gene and the AtSIZ gene. In conclusion, when 100 scales of lily cv. Red flame are bombarded, this study produced approximately 17-18 transgenic plantlets with an optimized bombardment protocol. The protocol described here can contribute to the breeding program of lilies.