• Title/Summary/Keyword: Marker Detection

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Detection of Individuals Restoring Fertility by DNA Fragment Converted into STS (sequence tagged site) in Red pepper

  • Lee, Jang-Soo;Lee, Keun-Hyang;Kim, Yeo-Song;Kang, Kwon-Kyoo;Nou, Ill-Sup;Hirata, Yutaka
    • Plant Resources
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    • v.7 no.2
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    • pp.136-140
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    • 2004
  • Co-segregation of male fertility with DNA markers selected by RAPD analysis as being potentially linked to the restorer gene (Rf) for Cytoplasmic male sterility (CMS) was analyzed using segregating F2 population. One RAPD marker directly linked to the Rf locus was identified. Amplification of OPT-02/570 using the STS primers generated a monomorphic band of each fertile plants randomly selected F2 progenies. From these results, this specific marker would be strongly linked to be restoring gene. The use of STS marker is effective in overcoming the reliability of the RAPD phenotype and improving their utility for MAS, co-dominant STS markers are especially very useful.

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Implementation of Automatic Target Tracking System for Multirotor UAVs Using Velocity Command Based PID controller (속도 명령 기반 PID 제어기를 이용한 멀티로터 무인항공기의 표적 자동 추종 시스템 구현)

  • Jeong, Hyeon-Do;Ko, Seon-Jae;Choi, Byoung-Jo
    • IEMEK Journal of Embedded Systems and Applications
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    • v.13 no.6
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    • pp.321-328
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    • 2018
  • This paper presents an automatic target tracking flight system using a PID controller based on velocity command of a multirotor UAV. The automatic flight system includes marker based onboard target detection and an automatic velocity command generation replacing manual controller. A quad-rotor UAV is equipped with a camera and an image processing computer to detect the marker in real time and to estimate the relative distance from the target. The marker tracking system consists of PID controller and generates velocity command based on the relative distance. The generated velocity command is used as the input of the UAV's original flight controller. The operation of the proposed system was verified through actual flight tests using a marker on top of a moving vehicle and tracks it to successfully demonstrate its capability using a quad-rotor UAV.

Development of drone flight control system using marker image processing technique (마커 영상처리기술을 이용한 드론 비행 제어 시스템 개발)

  • Yun, Tae-Jin;Jang, Jae-Ho;Ok, Ung-Seok;Kim, Jong-In;Choi, Da-Young
    • Proceedings of the Korean Society of Computer Information Conference
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    • 2020.01a
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    • pp.131-132
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    • 2020
  • 본 논문에서는 OpenCV의 Marker Detection 기술을 이용하여 특정지점의 마커를 영상처리기술로 인식하여 드론의 자동 이착륙 및 주변 위기상황, 미션수행 등을 마커를 통해서 드론에게 전달하여 비행 제어할 수 있는 체계를 개발한다. 드론은 OpenCV Aruco모듈을 이용하여 Marker ID별로 특정 명령어를 데이터 베이스와 비교하여 비행제어 명령을 수행한다. 지상에서는 마커의 변경을 통해서 실시간으로 미션변경을 할 수 있다. 이를 통해 드론은 제어용 송수신 채널을 통해서 통신을 하고는 있으나, 주파수 채널수가 제한이 되어 있으므로 구체적인 비행 제어 명령을 마커를 통해 이착륙시 추가적이며, 자동적인 진행이 가능하다.

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Choosing Optimal STR Markers for Quality Assurance of Distributed Biomaterials in Biobanking

  • Chung, Tae-Hoon;Lee, Hee-Jung;Lee, Mi-Hee;Jeon, Jae-Pil;Kim, Ki-Sang;Han, Bok-Ghee
    • Genomics & Informatics
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    • v.7 no.1
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    • pp.32-37
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    • 2009
  • The quality assurance (QA) is of utmost importance in biobanks when archived biomaterials are distributed to biomedical researchers. For sample authentication and cross-contamination detection, the two fundamental elements of QA, STR genotyping is usually utilized. However, the incorporated number of STR markers is highly redundant for biobanking purposes, resulting in time and cost inefficiency. An index to measure the cross-contamination detection capability of an STR marker, the mixture probability (MP), was developed. MP as well as other forensic parameters for STR markers was validated using STR genotyping data on 2328 normal Koreans with the commercial AmpFlSTR kit. For Koreans, 7 STR marker (D2S1338, FGA, D18S51, D8S1179, D13S317, D21S11, vWA) set was sufficient to provide discrimination power of ${\sim}10^{-10}$ and cross-contamination detection probability of ${sim}1$. Interestingly, similar marker sets were obtained from African Americans, Caucasian Americans, and Hispanic Americans under the same level of discrimination power. Only a small subset of commonly used STR markers is sufficient for QA purposes in biobanks. A procedure for selecting optimal STR markers is outlined using STR genotyping results from normal Korean population.

Relationship between erb-B2 mRNA Expression in Blood and Tissue of Invasive Ductal Carcinoma Breast Cancer Patients and Clinicopathological Characteristics of the Tumors

  • Moazzezy, Neda;Ebrahimi, Fatemeh;Sisakht, Mahsa Mollapour;Yahyazadeh, Hossein;Bouzari, Saeid;Oloomi, Mana
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.1
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    • pp.249-254
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    • 2016
  • Molecular detection methods such as RT-PCR for detecting breast cancer-associated gene expression in the peripheral blood have the potential to modify breast cancer (BC) staging and therapy. In this regard, we evaluated the potential of erb-B2 molecular marker in BC detection and analyzed the expression of erb-B2 mRNA in the peripheral blood and fresh tissue samples of 50 pretreated female BC patients and 50 healthy females by reverse transcription-PCR (RT-PCR) method. We also assessed the correlation of erb-B2 mRNA marker positivity in peripheral blood and tumor tissue samples with clinical and pathological factors in BC patients in order to evaluate its prognostic value. It was shown that there is a significant difference between healthy females and BC patients with expression of the erb-B2 molecular marker (p<0.01). A significant difference between the expression of erb-B2 in the peripheral blood and tissue samples of BC patients (p<0.01) and the frequency of circulating erb-B2 mRNA expression in peripheral blood and in tissue was detected by RT-PCR. No correlation was found between erb-B2 mRNA expression in blood or tumor tissue samples and lymph node, tumor grade, tumor stage, tumor size, patient's age, ki67, estrogen receptor (ER), progesterone receptor (PGR), P53, and HER-2 status. However, in a small subset of 31 BC patients we found that expression of erb-B2 in peripheral blood or in both peripheral blood and tumor tissue was directly correlated with lympho-vascular invasion and perineural invasion as poor prognostic features. The highest rates of erb-B2 expression in peripheral blood or tumor tissue were in the ER and PR negative and HER-2 positive group. This study suggests that the application of the RT-PCR and immunohistochemical methods for erb-B2 molecular marker detection would provide a higher detection rate, especially in early stage BC.

Improved Tooth Detection Method for using Morphological Characteristic (형태학적 특징을 이용한 향상된 치아 검출 방법)

  • Na, Sung Dae;Lee, Gihyoun;Lee, Jyung Hyun;Kim, Myoung Nam
    • Journal of Korea Multimedia Society
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    • v.17 no.10
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    • pp.1171-1181
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    • 2014
  • In this paper, we propose improved methods which are image conversion and extraction method of watershed seed using morphological characteristic of teeth on complement image. Conventional tooth segmentation methods are occurred low detection ratio at molar region and over, overlap segmentation owing to specular reflection and morphological feature of molars. Therefore, in order to solve the problems of the conventional methods, we propose the image conversion method and improved extraction method of watershed seed. First, the image conversion method is performed using RGB, HSI space of tooth image for to extract boundary and seed of watershed efficiently. Second, watershed seed is reconstructed using morphological characteristic of teeth. Last, individual tooth segmentation is performed using proposed seed of watershed by watershed algorithm. Therefore, as a result of comparison with marker controlled watershed algorithm and the proposed method, we confirmed higher detection ratio and accuracy than marker controlled watershed algorithm.

The Role Of Tumor Marker CA 15-3 in Detection of Breast Cancer Relapse After Curative Mastectomy (유방암 환자에서 근치적 유방 절제술 후 재발 발견에 대한 CA 15-3의 역할)

  • Hyun, In-Young;Kim, In-Ho;Lee, Moon-Hee;Kim, Chul-Soo
    • The Korean Journal of Nuclear Medicine
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    • v.38 no.4
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    • pp.311-317
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    • 2004
  • Purpose: The purpose of this study was to determine the utility of tumor marker CA 15-3 in the following: the diagnosis of breast cancer relapse after curative mastectomy, and the differentiation or the value of tumor marker by site of metastases. Materials and Methods: Two hundred two patients (median age 48 years) with breast cancer included in the follow-up after curative mastectomy. The tumor marker CA 15-3 was determined by IRMA (CIS BIO INTERNATIONAL, France). Test values > 30 U/ml were considered elevated (positive). Results: Among 202 patients, recurrent diseases were found in 16 patients. CA 15-3 was elevated in 5 of 16 patients with recurrences. There was no false-positive patient who had elevated CA 15-3. Sensitivity and specificity of CA 15-3 for detection of breast cancer recurrence were 31%, and 100%. CA 15-3 was elevated in all of the 4 patients with liver metastases. CA 15-3 was elevated in none of the patients who relapsed with metastasis to bone-only or contralateral breast-only. Conclusion: The tumor marker CA 15-3 in the detection of breast cancer relapse after curative mastectomy is specific, but not sensitive. However, it is useful to rule out liver metastases of breast cancer, which indicates bad prognosis.

Improved LiDAR-Camera Calibration Using Marker Detection Based on 3D Plane Extraction

  • Yoo, Joong-Sun;Kim, Do-Hyeong;Kim, Gon-Woo
    • Journal of Electrical Engineering and Technology
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    • v.13 no.6
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    • pp.2530-2544
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    • 2018
  • In this paper, we propose an enhanced LiDAR-camera calibration method that extracts the marker plane from 3D point cloud information. In previous work, we estimated the straight line of each board to obtain the vertex. However, the errors in the point information in relation to the z axis were not considered. These errors are caused by the effects of user selection on the board border. Because of the nature of LiDAR, the point information is separated in the horizontal direction, causing the approximated model of the straight line to be erroneous. In the proposed work, we obtain each vertex by estimating a rectangle from a plane rather than obtaining a point from each straight line in order to obtain a vertex more precisely than the previous study. The advantage of using planes is that it is easier to select the area, and the most point information on the board is available. We demonstrated through experiments that the proposed method could be used to obtain more accurate results compared to the performance of the previous method.

Detection of Circulating Melanoma Cells by a Two-marker Polymerase Chain Reaction Assay in Relation to Therapy

  • Bitisik, Ozlem;Camlica, Hakan;Duranyildiz, Derya;Tas, Faruk;Kurul, Sidika;Dalay, Nejat
    • BMB Reports
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    • v.36 no.2
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    • pp.173-178
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    • 2003
  • Malignant melanoma is one of the most rapidly increasing cancer types, and patients with metastatic disease have a very poor prognosis. Detection of metastatic melanoma cells in circulation may aid the clinician in assessing tumor progression, metastatic potential, and response to therapy. Tyrosinase is a key enzyme in melanine biosynthesis. The gene is actively expressed in melanocytes and melanoma cells. Melan A is a differentiation antigen that is expressed in melanocytes. The presence of these molecules in blood is considered a marker for circulating melanoma cells. In this study, we analyzed the usefulness of this marker combination I evaluating the response to therapy in the blood of 30 patients with malignant melanoma. Circulating cells were detected by a reverse-transcriptase-polymerase-chain reaction. The tyrosinase expression was observed in 9 (30%) patients and Melan A in 19 (63.3%) patients before therapy. Following treatment, the tyrosinase mRNA was detected in only one patient, while Melan A transcripts were still present in 14 patients. We suggest that this molecular assay can identify circulating melanoma cells that express melanoma-associated antigens and may provide an early indication of therapy effectiveness.

Evaluation of MT1XT20 Single Quasi-Monomorphic Mononucleotide Marker for Characterizing Microsatellite Instability in Persian Lynch Syndrome Patients

  • Farahani, Najmeh;Nikpour, Parvaneh;Emami, Mohammad Hassan;Hashemzadeh, Morteza;Zeinalian, Mehrdad;Shariatpanahi, Seyed Shervin;Salehi, Rasoul
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.9
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    • pp.4259-4265
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    • 2016
  • Background: Colorectal malignancies with high microsatellite instability (MSI-H), either hereditary (Lynch syndrome) or sporadic, demonstrate better prognosis and altered response to 5FU chemotherapy. It is now recommended to perform MSI testing for all new cases of colorectal cancer regardless of being categorized as hereditary or sporadic. For MSI detection, immunohistochemistry or PCR-based protocols using a cohort of various sets of STR markers are recommended. Here we aimed to evaluate a simplified protocol using just a single STR marker, MT1XT20 mononucleotide repeat, for detection of MSI in Lynch syndrome patients. A Promega five-marker MSI testing panel and immunohistochemistry (IHC) were used as the gold standard in conjunction with MT1XT20. Materials and Methods: Colorectal patients with a positive history of familial cancers were selected by evaluating medical records. Based on Amsterdam II criteria for Lynch syndrome 20 families were short listed. DNA was extracted from formalin fixed paraffin embedded tumour and adjacent normal tissues resected from the index case in each family. Extracted DNA was subjected to MT1XT20 mononucleotide marker analysis and assessment with a commercially available five marker MSI testing kit (Promega, USA). IHC also was performed on tissue sections and the results were compared with PCR based data. Results: Eight (40%), seven (35%) and five (25%) cases were MSI positive using with the Promega kit, IHC and MT1XT20, respectively. Among the markers included in Promega kit, BAT26 marker showed instability in all 8 samples. NR24 and NR21 markers showed instability in 7 (87.5%), and BAT25 and MONO 27 in 6 (75%) and 5 (62.5%). Conclusions: Although MT1XT20 was earlier reported as a valid standalone marker for MSI testing in CRC patients, we could not verify this in our Iranian patients. Instead BAT26 among the markers included in Promega MSI testing kit showed instability in all 8 MSI-H CRC samples. Therefore, it seems BAT26 could act well as a single marker for MSI testing in Iranian CRC patients.