• 한국어류학회지
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• 제19권1호
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• pp.44-50
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• 2007

도루묵의 식성을 조사하기 위해 강원도 삼척 연안해역에서 기선저인망을 이용하여 2003년 4월부터 2004년 3월까지 매월 채집된 2,115개체의 도루묵 위내용물을 분석하였다. 도루묵의 가장 중요한 먹이생물은 단각류(Amphipoda)였으며, 다음으로 곤쟁이류(Mysidacea), 두족류(Cephalopoda), 어류(Pisces)가 중요한 먹이생물이었다. 그 밖에 난바다곤쟁이류(Euphausiacea), 난(Eggs), 이매패류(Bivalvia), 선형동물(Nematoda), 해초류(Seagrass) 등도 도루묵의 위내용물 중에서 발견되었으나, 그 양이 매우 적었다. 도루묵은 성장함에 따라 먹이생물 조성의 변화를 보였는데, 가장 작은 크기군인 체장 9~10 cm 크기군에서는 곤쟁이류와 단각류가 중요한 먹이생물이었다. 도루묵의 크기가 더욱 증가함에 따라 체장 16~23 cm 크기군에서는 단각류의 점유율은 더욱 증가하였으며, 두족류와 어류의 점유율이 증가하였다.

• 한국발생생물학회지:발생과생식
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• 제16권1호
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• pp.19-29
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• 2012

The ultrastructural characteristics of germ cell differentiations during spermatogenesis and mature sperm morphology in male $Atrina$ ($Servatrina$) $pectinata$ were evaluated via transmission electron microscopic observation. The accessory cells, which contained a large quantity of glycogen particles and lipid droplets in the cytoplasm, are assumed to be involved in nutrient supply for germ cell development. Morphologically, the sperm nucleus and acrosome of this species are ovoid and conical in shape, respectively. The acrosomal vesicle, which is formed by two kinds of electron-dense or lucent materials, appears from the base to the tip: a thick and slender elliptical line, which is composed of electron-dense opaque material, appears along the outer part (region) of the acrosomal vesicle from the base to the tip, whereas the inner part (region) of the acrosomal vesicle is composed of electron-lucent material in the acrosomal vesicle. Two special characteristics, which are found in the acrosomal vesicle of A. ($S$) $pectinata$ in Pinnidae (subclass Pteriomorphia), can be employed for phylogenetic and taxonomic analyses as a taxonomic key or a significant tool. The spermatozoa were approximately $45-50{\mu}m$ in length, including a sperm nucleus (about $1.43{\mu}m$ in length), an acrosome (about $0.51{\mu}m$ in length), and a tail flagellum (about $46-47{\mu}m$). The axoneme of the sperm tail evidences a 9+2 structure.

• 환경생물
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• 제28권4호
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• pp.179-187
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• 2010

본 연구는 가거도, 소화도 및 독도해역에 대한 2009년 8월의 조하대 암반조사를 통해서 해양생태계의 중요 구성생물 중의 하나인 대형무척추동물의 생물다양성, 생물량 및 군집구조를 파악하고, 이를 기초로 도서생물지리설(The Theory of Island Biogeography)의 관점에서 3개섬에 서식하는 대형무척추동물의 유사성 정도를 검증하였다. 연구해역에서 출현한 대형무척추동물은 총 104종, 7,176개체(399 inds. $m^{-2}$)와 27,120.68 g (1,506.70 g $m^{-2}$)이었다. 연체동물류는 출현 종수, 개체수 및 생체량에 있어서 각각 전체의 44.23%, 41.19% 및 90.32%를 점유하여 가장 우점하는 생물군이었다. 연구해역에서 출현한 대형무척추동물의 개체수와 생체량을 근거로 상위 10위까지의 우점종을 선정하였다. 개체수에 기초한 상위 10위 우점종들이 차지하는 밀도는 4,820개체로 전체의 67.17%를 점유하였다. 특히 절지동물 갑각류의 미동정 옆새우류(Amphipoda spp.)와 연체동물 이매패류의 개적구(Modiolus agripetus)는 각각 1,580개체, 22.02%와 1,228개체, 17.11%를 점유하여 단일 종으로 가장 우점하는 생물이었다. 생체량에 있어서도 상위 우점종들이 나타내는 생체량은 25,942.80 g으로 전체의 95.66%를 차지하였다. 이 가운데 이매패류의 홍합 (Mytilus coruscus)과 태생굴(Striostrea circumpicta)은 각각 15,167.64 g과 4,781.16 g을 나타내 55.93%와 17.63%의 높은 점유율을 보였다. 연구해역에서 출현한 대형무척추동물의 개체수를 기초로 군집분석을 실시한 결과, 군집은 크게 2개의 그룹으로 나뉘었다. 그룹 1에는 가거도 정선 2의 하부 수심 정점을 제외한 가거도와 독도의 모든 정점이 포함되었고, 그룹 2는 이를 제외한 정점들로 구성되었다. 연구해역에서 갑각류의 미동정의 옆새우류, 바다대벌레류 및 성구동물의 상어껍질별벌레(Phascolosoma scolops)가 공통적으로 우점하였다. 이를 제외하면, 그룹 1에서는 다모류의 고리털갯지렁이(Eunice antennata), Syllidae unid., 갑각류의 만각류에 속하는 빨강따개비(Megabalanus rosa) 및 홍합의 밀도가 높았다. 반면 그룹 2에서는 갑각류의 집게류에 속하는 갯가게붙이(Petrolisthes japonicus), 연체동물 복족류의 햇살밤고둥 (Lirularia pygmaea) 및 완족동물의 세로줄조개사돈(Coptothyris grayi)이 대표적인 생물이었다. 한편 본 연구에서 출현 종수는 소화도에서 가장 많았고, 다음으로 가거도와 독도의 순이었다. 각 해역의 면적과 육지와의 떨어짐 정도를 기초로 도서생물지리설에 근거하면, 연구해역에서의 생물다양성은 도서생물지리설에 정확하게 일치하는 것은 아니지만 비교적 잘 부합되는 것으로 추정된다.

• 한국패류학회지
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• 제30권4호
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• pp.333-342
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• 2014

Ultrastructural studies of oogenesis in oocytes, oocyte degeneration associated with the follicle cells in female Coecella chinensis were investigated for clams collected from Namhae, Geongsangnam-do, Korea. In this study, vitellogenesis during oogenesis in the oocytes occured by way of endogenous autosynthesis and exogenous heterosynthesis. Of two processes of vitellogenesis during oogenesis, the process of endogenous autosynthesis involved the combined activity of the Golgi complex, mitochondria and rough endoplasmic reticulum. whereas the process of exogenous heterosynthesis involved endocytotic incorporation of extraovarian precursors at the basal region of the oolema of the early vitellogenic oocytes prior to the formation of the vitelline coat. It is assumed that the follicle cells were involved in the development of previtellogenic and early vitellogenic oocytes and appear to play an integral role in vitellogenesis in the early and late vitellogenic oocytes by endocytosis of yolk precursors, and also they were involved in oocyte degeneration by assimilating products originating from the degenerated oocytes, thus allowed the transfer of york precursors needed for vitellogenesis (through phagocytosis by phagolysosomes after spawning). Follicle cells presumably have a lysosomal system for breakdown products of oocyte degeneration. and for reabsorption of various phagosomes (phagolysosomes) in the cytoplasm for nutrient storage during the period of oocyte degeneration.

• Animal cells and systems
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• 제13권3호
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• pp.339-344
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• 2009

This study aims to find out the effect of heavy metals, as is the case of EDCs (endocrine disrupting chemicals), on reproductive endocrine disruption of aquatic animals. In the present experiment zinc, which is a heavy metal well known for its androgenous activity, was used. The experimental period was 24 weeks, starting in November during the inactive stage of the clam's reproductive cycle. The experimental groups were composed of one control condition and three zinc exposure conditions (0.64, 1.07, and 1.79 mg/L). The sex ratio (F:M) was 1:1.06 in the control group and 1:1.70 in all the exposed group, illustrating the tendency for higher proportion of males with increases in zinc concentration. Gonad maturity was higher in 1.07, and 1.79 mg/L groups compared to the control group, with higher maturity observed in males than females. Intersex individuals made up 24.7% of the exposed group, while females exhibited a higher ratio than the males with increasing zinc concentration. The results of this study indicate that zinc functions as an androgenic effector on the reproduction of Gomphina veneriformis.

• Ocean Science Journal
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• 제41권4호
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• pp.211-219
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• 2006

A comparison was made of population of the economically important cockle Anadara (=Scapharca) broughtoni (Bivalvia, Arcidae) inhabiting different areas of the Razdolnaya River estuary at the head of Amurskii Bay (Peter the Great Gulf, East Sea). Also, changes in cockle population density and structure, as well as in cockle growth rates during the last 20 years were studied. In all years of investigation, the morphometrical parameters and growth rates of cockles were smaller at the sites located close to the River mouth than farther down-estuary. The differences can be attributed to higher concentration of suspended particulate matter, decreased salinity and water temperature, as well as a longer exposure to these unfavorable environmental factors at sites located close to the River mouth, compared to farther sites. For two decades, cockle population density had decreased by almost 30 times at some sites in the River estuary. The main reason for this population decline is commercial over-fishing of the cockle. Besides, for the last 20 years, linear parameters of the cockles in the population decreased approximately by 30% and weight parameters, almost two times. Cockle growth rates also decreased for this period. Evidently, these facts are due to the damaging effect of dredging.

• 한국발생생물학회지:발생과생식
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• 제15권3호
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• pp.239-247
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• 2011

Spermatogenesis and taxonomic values of mature sperm morphology of in male Septifer (Mytilisepta) virgatus were investigated by transmission electron microscope observations. The morphologies of the sperm nucleus and the acrosome of this species are the cylinder shape and cone shape, respectively. Spermatozoa are approximately 45-50 ${\mu}m$ in length including a sperm nucleus (about 1.26 ${\mu}m$ long), an acrosome (about 0.99 ${\mu}m$ long), and tail flagellum (about 45-47 ${\mu}m$). Several electron-dense proacrosomal vesicles become later the definitive acrosomal vesicle by the fusion of several Golgi-derived vesicles. The acrosome of this species has two regions of differing electron density: there is a thin, outer electron-dense opaque region (part) at the anterior end, behind which is a thicker, more electron-lucent region (part). In genus Septifer in Mytilidae, an axial rod does not find and also a mid-central line hole does not appear in the sperm nucleus. However, in genus Mytilus in Mytilidae, in subclass Pteriomorphia, an axial rod and a mid-central line hole appeared in the sperm nucleus. These morphological differences of the acrosome and sperm nucleus between the genuses Septifer and Mytilus can be used for phylogenetic and taxonomic analyses as a taxonomic key or a significant tool. The number of mitochondria in the midpiece of the sperm of this species are five, as seen in subclass Pteriomorphia.

• Animal cells and systems
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• 제11권2호
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• pp.227-234
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• 2007

Spermatogenesis, the reproductive cycle, and the size at first sexual maturity in male Mactra chinensis were investigated by cytological and histological observations. The spermatozoon exhibits a primitive type morphology and is similar to those of other bivalves in that it contains a short midpiece with four mitochondria surrounding the centrioles. The morphologies of the sperm nucleus type and the acrosome shape of this species are cylindrical and modified cap-like, respectively. The spermatozoon is approximately $40-45\;{\mu}m$ in length including the sperm nucleus (about $1.46\;{\mu}m$), acrosome (about $1.20\;{\mu}m$) and tail flagellum. The axoneme of the sperm tail flagellum consists of nine pairs of microtubules at the periphery and a pair at the center. The axoneme of the sperm tail shows a 9+2 structure. The spawning period of this species lasts from June to September, and the main spawning occurs in July and August, when the seawater temperature is greater than $20^{\circ}C$. The percentage of individual male clams at first sexual maturity was 56.5% for those whose shell lengths were 35.1-40.0 mm, and 100% for over 45.1 mm. Accordingly, harvesting clams <35.1 mm in shell length could potentially cause a drastic reduction in recruitment, and a measure indicating a prohibitory fishing size should be taken for adequate fisheries management.

• 한국패류학회지
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• 제24권2호
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• pp.97-104
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• 2008

The gonad index, reproductive cycle and first sexual maturity of Sinonovacula constricta collected from Simpo, Kimje-gun, Korea were investigated by histological analysis. The gonad index(GI) in both sexes of S. constricta increased from April and reached a maximum in July when the water temperature rapidly increased. And then, the GI values gradually decreased by spawning from August through October. Monthly variations in the GI showed a close relationship with ovarian development. The reproductive cycle in females and males can be classified into five successive stages: early active stage(March to June), late active stage(May to July), ripe stage(July to September), partially spawned stage(August to October), spent/inactive stage(October to March). The percentage of first sexual maturations in female and male clams of 50.1-60.0 mm in shell length was over 50%, and for clams over 70.1 mm in shell length, it was 100%. Because harvesting clams < 50.1 mm in shell length could potentially cause a drastic reduction in recruitment, a measure including a prohibitory fishing size should be taken for adequate improved fisheries resource management.

• Animal cells and systems
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• 제14권4호
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• pp.343-349
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• 2010

Ultrastructural studies of vitellogenesis in oocytes and follicle cells during oogenesis in female Protothaca (Notochione) jedoensis were investigated by histological and transmission electron microscope observations. In early vitellogenic oocytes, combined activities of the Golgi complex, mitochondria and rough endoplasmic reticulum in the cytoplasm are associated with autosynthetic vitellogenesis. Furthermore, at this time, many coated vesicles at the basal region of the oolemma of the oocyte lead to the formation of vesicles through endocytosis in the cytoplasm. Through the formation of the coated pits on oolemma during vitellogenesis, the uptake of extrafollicular precursors (nutritive materials) occurs in coated vesicles by endocytosis. Therefore, it is assumed that these exogenous materials are involved in heterosynthetic vitellogenesis. During late oogenesis, exogenous yolk precursors (yolk granules), lipid droplets and proteinaceous yolk granules are present in the cytoplasm of late vitellogenic oocytes. In mature oocytes, small yolk granules appear intermingled and form large mature yolk granules. Thus, two processes of vitellogenesis occur in oocytes by way of endogenous autosynthesis and exogenous heterosynthesis. The follicle cells attached to the oocytes appear to play an integral role in vitellogenesis in this study.