• Journal of Aquaculture
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• v.22 no.1
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• pp.34-41
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• 2009

Gonadal development and reproductive cycle of the granular ark Tegillarca granosa were investigated by histological observations. Samples were collected monthly from January to Decemberry 2002 in the Yeoja Bay of Yeosu, Jeollanam-do, Korea. T. granosa was dioecious. The gonads consist of a number of oogenic follicle and acinus. Monthly changes in the gonad index reached a maximum in June and a minimum in August. Monthly changes in the condition index reached a maximum in July and a minimum in September. The reproductive cycle of this species can be divided into five successive stages: early active stage (March to May), late active stage (April to June), ripe stage (May to July), spent stage (July to August) and recovery and resting stage (September to March). The spawning of T. granosa occurred in July and August in Yeoja Bay. The sex ratio of female to male was not significantly different from 1:1.

• Journal of Aquaculture
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• v.22 no.1
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• pp.63-67
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• 2009

In order to establish optimum anesthesia concentration, we tested the efficacy of clove oil at five different concentrations in large sized (mean SL $17.1{\pm}2.21\;cm$) and small sized (mean SL $0.6{\pm}0.06\;cm$) dark-banded rockfish Sebastes inermis. Optimal anesthesia concentration for dark-banded rockfish was $150\;mgL^{-1}$ in both large and small sized fish. In general, fish exposed to higher anesthetic doses were rapidly induced but took longer to recover (P<0.05). Recovery time of small sized fish was longer than large sized fish in lower concentrations, while recovery time of large sized fish was longer than small sized fish in higher concentration (P<0.05). Using the established optimum aesthetic concentration, we evaluated the physiological response of dark-banded rockfish to clove oil by measuring plasma cortisol and glucose levels. Following administration of $150\;mgL^{-1}$ clove oil at $20^{\circ}C$ (optimum breeding temperature), plasma cortisol level was highest ($42.2{\pm}11.318{\mu}g/dL$) after 0 hour, while plasma glucose level was highest ($52.5{\pm}10.61\;mg/dL$) after 1 hour. Plasma cortisol and glucose concentrations required 6 and 2 hours, respectively, to return to pre-exposure levels.

• Journal of Marine Bioscience and Biotechnology
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• v.3 no.1
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• pp.1-6
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• 2008

Angola has a coastline of about 1,650 km long. Two diverging current namely, the Angola current with its warm water from the north and the cold Benguella Current in the south create a strong up-welling with a high productive ecosystem for marine resources. The area from Lobito to the mouth of the Cunene River, also known as the Southern fishing zone is by far the most productive of Angola's fishing zones. In 1977, the total potential of its marine fisheries sector was estimated at more than 700,000 tonnes per annum. In 2003, the Total Allowable Catch (TAC) established for demersal species was 57,600 tonnes and 160,000 tonnes for pelagic species. The most important resources are various marine demersal and pelagic fish including pilchard and the Cape and Cunene horse Mackerel (Tranchurus capensis and T. trecae). Sardinellas (Sardinella aurita and S. maderensis) are fished in parallel with horse mackerel. The rest of the catches are mainly demersal spp. and some deep water crustaceans. The demersal sppecies consist of Hake (Merluccius polli and M. capensis) and the large eye dentex spp. Tunas are caught at certain times of the year whilst some marine shrimp are also harvested from the Angolan waters. Angola also has several high value freshwater fish species, exploited by about 255 fishers. Tilapia sp. is among the most important and abundant fresh water fish found in Angola. Other species include the catfish (Clarias gariepinus) and fresh water prawns (Macrobrachuin rosenbergii). Some aquaculture ponds have been established in the country side, but due to lack of investment, proper training and the impact of civil war have seriously limited developments in the sub sector.

• Ocean and Polar Research
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• v.42 no.4
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• pp.303-311
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• 2020

In this study, the embryonic development and hatchability of eggs fertilized by the reciprocal hybrids of kelp grouper (Epinephelus bruneus) and red-spotted grouper (E. akaara) were evaluated with the goal of establishing a novel hybrid with enhanced growth and viability during the farming period in the temperate waters of Korea. The fertilization rates were lower for hybrids than for maternal purebreds and were significantly higher in the red-spotted grouper ♀ × kelp grouper ♂ hybrid (RGKG, 89.61 ± 1.58%) than in the kelp grouper ♀ × red-spotted grouper ♂ hybrid (KGRG, 74.82 ± 4.23%, p < 0.05). Unlike the fertilization rates, the hatching rates of fertilized eggs were similar between hybrids and maternal purebreds and did not differ significantly between KGRG and RGKG (72.74 ± 3.60% vs. 75.23 ± 2.20%, respectively, p > 0.05). The embryonic development of the hybrids was similar to that of maternal purebreds; however, irregular cleavage and asymmetric blastoderm were noticeable in the developing eggs of KGRG hybrids. The deformity rates of newly hatched larvae were higher in hybrids than in maternal purebreds and were significantly higher in KGRG than in RGKG (17.47 ± 1.28% vs. 7.11 ± 0.54%, respectively, p < 0.05). These results demonstrate the potential to produce viable larvae from these two hybrids. Although the production efficiency of KGRG was lower than that of RGKG, the fertilization, hatching, and deformity rates make both hybrids useful for further comparative studies regarding economic aspects.

• Development and Reproduction
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• v.13 no.4
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• pp.281-289
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• 2009

The slime flounder Microstomus achne were caught at Geomun Island, Yosu-si, Jeollanamdo from January to March in 2006. The fertilized eggs were observed for morphological development of egg, embryo and larva. Eggs were colorless transparent, separative pelagic, absent of oil globule, and the diameter was 1.64${\pm}$0.03 mm (n=50). The eggs were hatched at 168 hours 40 minutes after fertilization in the range of $9.8\sim13.0^{\circ}C$ (mean $11.4{\pm}1.6^{\circ}C$). Total length of newly hatched larva was 4.05${\pm}$0.18 mm (n=20). The larva had developed membranous fin showing waterdrop-shaped structure, and their mouth and anus were not open. The myotomes were 14~15+33~34=47~49. The egg yolks were 1.64${\pm}$0.12 mm in major axis, and 1.23${\pm}$0.19 mm in minor axis. At 12 days after hatching, the total length was 7.32${\pm}$0.42 mm(n=20). The egg yolk was completely absorbed and transferred to post larval stage. Star-shaped melanophores and branch-shaped xanthophores in the edge of membranous fin were more densed. Chrysanthemum-shaped melanophores in the notochord were densed and formed 4~5 melanophore bands. At 90~93 days after hatching, morphological features of the larva, 19.91${\pm}$1.63 mm TL(n=20), were transferred to juvenile stage showing similar features with those of the adult fish.

• Development and Reproduction
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• v.12 no.2
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• pp.195-202
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• 2008

The gonadosomatic index, germ cell differentiation, and the ovarian cycle in female jicon scallop, Chlamys farreri were studied by histologic and cytologic observations. In the early vitellogenic oocyte, the Golgi complex, mitochondria and rough endoplasmic reticulum were involved in the formation of lipid droplets. In the late vitellogenic oocyte, exogenous substances, namely, glycogen particles and lipid granular substances appeared in the germinal epithelium passed into the ooplasm through the microvilli of the envelope. Yolk granules and multivesicular bodies were involved in the formation of proteinecious yolk granules in the late vitellogenic oocyte. Vitellogenesis occurrs by endogenous autosynthesis and exogenous heterosynthesis. The auxiliary cells function as nutritive cells in the formation and development of the previtellogenic and early vitellogenic oocytes in their earlr stages. Monthly changes in the gonadosomatic index were closely associated with ovarian developmental phases. The reproductive cycle of this species can be classified into five stages: early active stage (January to March), late active stage (March to April), ripe stage (April to August), partially spawned stage (June to August), and spent/inactive stage (August to December). Spawning occurred from June to August, and the major spawning season was from July to August when the sea water was at high temperature.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.3
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• pp.262-267
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• 2009

Two 15-week feeding trials were conducted to investigate feeding rate and feeding frequency for growth of sub-adult flounder in suboptimal water temperature ($12^{\circ}C$). In the first experiment, duplicated groups of fish (initial mean weight of 279 g) were fed a extruded pellet once a day at the different feeding rates of 0.1, 0.15, 0.2, 0.3% body weight per day (BW/d), and satiation (0.32% BW/d). Weight gain and feed efficiency were increased significantly (P<0.05) with increase in feeding rates from 0.1 to 0.3% BW/d, but there were no significant differences in weight gain and feed efficiency of fish fed the diet between 0.3% BW/d and to satiation. Crude protein content of dorsal muscle of fish fed the 0.1% BW/d diet was significantly (P<0.05) lower than that of the other groups. In the second experiment, duplicated groups of fish (initial mean weight of 281 g) were fed to apparent satiation with a extruded pellet at three different feeding frequencies (one meal every two days, one meal a day and two meals a day). Weight gain, feed efficiency and condition factor of fish fed one meal every two days were significantly (P<0.05) lower than those of fish fed one meal and two meals a day, but there were no significant differences in weight gain, feed efficiency and condition factor of fish fed between one meal and two meals a day. Based on the results of this study, optimum feeding frequency is one meal a day with satiation feeding for growth and feed efficiency of sub-adult flounder (270-350 g) in suboptimal water temperature ($12^{\circ}C$).

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.4
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• pp.331-339
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• 2010

Shrimp farming is the most important mariculture industry on the west coast of South Korea. However, it has suffered from mass mortality due to viral disease outbreaks and coastal pollution due to water discharge. This study developed an intensive shrimp culture method for outdoor ponds, without water exchange, which minimizes the chance of viral transmission from the environment, reduces coastal pollution by water discharge and enhances shrimp production. A culture trial was conducted in two high-density polyethylene (HDPE)-lined ponds with a $550\;m^2$ surface area. The ponds were stocked with postlarvae of Litopenaeus vannamei, the major farmed shrimp species in Korea, on July 10, 2007, and cultured for 90 days with no water exchange. The stocking density of the postlarvae (B.W. 0.0015 g) was $272\;ind./m^2$, which is eight times higher than in traditional pond culture in Korea. At harvest, the total production of ponds 1 and 2 was 1,362kg ($2.48\;kg/m^2$) and 1,282 kg ($2.33\;kg/m^2$), respectively. This is 20~22 times higher than the mean farmed shrimp production ($0.112\;kg/m^2$) in Korea and about eight times higher than in traditional ponds with a good harvest. Although there was no water exchange throughout the culture period, the mean concentrations of unionized ammonia and nitrite-nitrogen were as low as 0.038 and 6.0 mg/L, respectively. The feed conversion rate (FCR) was 1.38, which is 20~45% lower than that of traditional pond cultures. The high efficiency of the diet in this study is thought to be due to a well-managed feeding strategy and well-developed bioflocs used as diet additions for the shrimp. The final body weight of the shrimp at harvest was low (12.2~12.5 g), compared with that of traditional pond culture. This may have resulted from the combination of a short culture period, high density of shrimp, and low temperature. This study suggests that a super-intensive shrimp pond culture method using biofloc technology with no water exchange can minimize viral transmission via water exchange, reduce coastal pollution, and enhance shrimp production.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.2
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• pp.217-225
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• 1998

This study was performed to compare the effects of a fish cage on the structure of meiobenthic communities at two locations during May, 1996. The number of meiofaunal taxa at the existing cage site and control site was 15 and 26, respectively. Nematoda was the most dominant group of meiobenthos. Nematoda and Sarcomastigophora comprised more than $90\%$ of total meiofauna at both sites. The total density of control site was 7,702 inds./$10\;cm^{2}$ which is 10 times more than that of fish cage site. The density was highest in the upper 1 cm and decreased with sediment depth. The nematodes/harpacticoid copepods ratio as an index of pollution monitoring for benthic ecosystem at fish cage site was two times higher than at control site. Kinorhyncha known to be sensitive to pollution stress was not found at fish cage site.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.17 no.1
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• pp.68-80
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• 1984

Garolim Bay is not only important fishing ground but also expected area for the tidal power plant. The construction and operation of tidal power plant will make change the ecosystem of this bay. Therefore, the actual fisheries stocks should be precisely understood for the effect estimation and overall utilization of the bay after the construction of the tidal power plant. During the study period from January through December in 1981, forty-six adult fishes species, 3 species of fish egg and 25 fishes larvae species have occurred in the bay. Considering the result on monthly distribution of eggs and larvae, the inner area of the bay seems to be important as nursing ground of larvae spawned at the outside bay in winter, e. g., Ammodytes personatus, and Enedrias sp. This inner bay is also major spawning ground for many species spawning in spring and summer, e. g., Gobiidae, Konosirus punctatus, Engraulis japonica, etc. Taking into consideration the annual mean production for three years($1978{\sim}1980$), there are two major fishing seasons. The one is in May-June for Enedrias larvae stock, and the another in October-November for big eyed herring stock. For the mariculture stocks, short necked clam, oyster and laver are important species. After construction of the tidal power plant, the migratory species, i. e., larvae of Enedrias and Ammodytes personatus, Mugil cephalus, Konosirus punctatus, etc. will be directly damaged by the interuption of migration route. On the otter hand, the change of physico-chemical factors of seawater will also affect the ecosystem of the bay. Consequently, for the overall utilization of the bay after construction, the actual ecosystem including the fisheries stocks, must bs precisely revealed, and the mechanical designs, e. g., sluice position and its demension, should be also considered with these biological characters of the bay.