• 제목/요약/키워드: Manual count

검색결과 26건 처리시간 0.029초

자동혈구분석기 XE-2100, ADVIA-120와 Manual Differential Count의 상관성 및 Morphology Flag 평가 (Correlation of XE-2100, ADVIA-120 and Manual Differential Count and Evaluation of Morphology Flag)

  • 이범희;변남섭;지명석;송순영;유선우;박효순
    • 대한임상검사과학회지
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    • 제36권2호
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    • pp.144-152
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    • 2004
  • With technological advances in automatic hematology analyzers, primary and screening differential counts of white blood cells (WBC) are done with automatic hematology analyzers. They are using different measurement and analysis principles, so differences in WBC differentials and WBC morphology flag exist. This study was carried out to analyze WBC differential counts and WBC morphology flags comparing them with the manual method. Patient EDTA samples in Vacutainer requested for WBC differentials were analyzed with XE-2100. And those samples with suspect flags messages index over 100 were selected and were analyzed with ADVIA-120. Peripheral blood smear film was subsequently made. Three investigators counted 200 cells each (600 cells) in 111 Wright-Giemsa stained blood films. Between two automatic hematology analyzers, neutrophil, lymphocyte, eosinophil, and monocyte showed good correlations, but basophil had moderate correlation. Among automatic hematology analyzers and manual count, neutrophil, lymphocyte, and eosinophil had good correlations, but monocyte had moderate correlation. XE-2100 had higher monocyte, which was due to atypical lymphocyte and myeloblast. LUC in ADVIA-120 was not due to monocyte in XE-2100. Morphology flagging rates were 146.9% in XE-2100 and was 93.2% in ADVIA-120. Positive predictive values of morphology flag were 58.2% in XE-2100 and 54.4% in ADVIA-120. Flags such as atypical lymphocyte, immature granulocyte, and left shift had higher predictive values and those such as N-RBC, platelets clump, and blast had lower ones. Between automatic hematology analyzers, WBC differentials showed good correlations. Predictive values for morphology flags can be variable with changing criteria. Reviewing criteria for WBC differentials and morphology flags should be established in each laboratory with regards to size of laboratory and patients it serves.

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Coulter® LH 750TM에 의한 백혈구 감별계산 및 Suspect Flags의 평가 (Evaluation of Coulter® LH 750TM on Leukocyte Differential Count and Suspect Flags)

  • 홍승복;최종태;이성규;김현수
    • 대한임상검사과학회지
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    • 제37권3호
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    • pp.143-148
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    • 2005
  • Automatic hematology analyzers provide the leukocyte differential count and the useful flags of the various hematological parameters. We compared LH 750 (Beckman Coulter Co., Miami, FL, USA) with manual method on differential leukocyte counts and evaluated the usefulness of the suspect flags, provided by this instrument. The comparison of leukocyte differential counts between two methods showed good correlation coefficient (r), which were 0.95 (neutrophil), 0.92 (lymphocyte), 0.82 (monocyte) and 0.95 (eosinophil). The frequency of the total flags displayed on LH 750 were 15.5%, which included immature granulocyte/left shift 63.5%, nucleated RBC 14.6%, platelet clumps 13.1%, variant lymphocyte 50% and blast 16.6%. This instrument showed higher positive predictive value in the flags such as platelet clumps 68.8%, immature granulocyte/left shift 61.5%, nucleated RBC 27.3%, variant lymphocyte 50% and blast 16.7%. In this study, the leukocyte differential counts of LH 750 showed good correlation with manual method and the suspect flags also showed a good performance for applying the criteria of re-examination in the clinical laboratory.

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A Comparison of Complete Blood Cell Count in Canine Blood Samples Obtained from the Jugular Vein, Cephalic Vein and Lateral Saphenous Vein

  • An, Hyung-Mo;Song, Joong-Hyun;An, Su-Jin;Yu, Do-Hyeon;Kim, Young Joo;Han, Donghyun;Jung, Dong-In
    • 한국임상수의학회지
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    • 제36권6호
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    • pp.314-318
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    • 2019
  • The purpose of this study was to compare the results of complete blood cell count (CBC) of blood samples collected from the jugular vein, cephalic vein and lateral saphenous vein and to find out if there were clinically significant differences. Total of 40 dogs were tested. CBC tests were conducted with blood samples obtained from the jugular vein, cephalic vein and lateral saphenous vein and manual differential count was performed to accurately distinguish the white blood cell (WBC) types. The results were analyzed using Repeated Measures ANOVA and posthoc test was conducted using the least significant difference method. As a result, there was a statistically significant difference (P < 0.05) in the total WBC and monocyte count. The post-hoc test of total WBC counts revealed a significant difference between the jugular vein and cephalic vein, and the jugular vein and lateral saphenous vein. For monocyte counts, a significant difference was observed between the jugular vein and lateral saphenous vein.

Recent Development of Rapid and Automation Technology for Food Microbiological Examination

  • Hiroshi Kurata
    • 한국식품위생안전성학회:학술대회논문집
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    • 한국식품위생안전성학회 1996년도 제11회 학술대회 및 정기총회 - 식품의 위생 안전성에 관한 최근 연구 동향
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    • pp.33-33
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    • 1996
  • Interests in the field of rapid methods and automation in microbiology have been growing steadily on an international scale in recent years. International meetings concerned this problem have been held in elsewhere in the world countries since the past twenty years. But, unfortunately in the field of microbial examination in food hygiene, this problem have not yet been developed so much as in the field of clinical microbiology. Today, I would like to introduce you here present aspects of rapid and automation technologies, those which are manly carrying in milk and meats industries. My illustration will be given recent improved technologies using automatic apparatus and instruments along with process of microbial count procedure. Recent direct microbiological counting system (ChemeScan \ulcorner) as real time ultrasensitive analysis created by Cheminex Ltd., France is now most evolutional instrument to provide direct microbial counts, down to one cell, within 30 minutes. The results from these evaluations how a good correlation between the ChemScan system and the standard plate count method. This system will be successful application for not only in the field of pharmacology but also food microbiology. In addition, current identification of microbes by sophisticated instruments suitable for food microbiology, one of which Biology is manual system (BIOLOG\ulcorner), provides reference-level capability at a modes price. For the manual system, the color reactions in the microplate are read by eye and manually keyed into personal computer. Species identification appears on the computer screen within seconds, along with biotype patterns, a list of closely related species, and other useful statistics. In present this is useful application for microbial ecology and epidemiological survey. RiboPrinter system newly produced by DuPont is now focusing among microbiologists in the world, and is one of the biggest microbial characterization system using a DNA-based approach. The technology analyzer is bacterial culture for its genetic fingerprint or riboprint pattern. Finally Bio-cellTracer system for automatic measurement of fungal growth and Fukitori-Maseter, a Surface Hygiene Monitoring Kit by using swabe procedure in food processing environment are briefly illustrated in this presentation.

Test turnaround Time for Complete Blood Cell Count using Delta and Panic Value Checks and the Q-flag Limit

  • Koo, Bon-Kyung;Ryu, Kwang-Hyun;Lim, Dae-Jin;Cho, Young-Kuk;Kim, Hee-Jin
    • 대한임상검사과학회지
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    • 제44권2호
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    • pp.66-74
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    • 2012
  • Test turnaround time (TAT) is the lead time from reception to reporting. In the complete blood cell count (CBC), 4 units of the XE-2100 (Sysmex Corp., Japan) processed around 80% of quantity, 1 unit of the LH-780 (Beckman-Coulter Incorp., USA) processed around 10% and 1 unit of ADVIA-2120 (Siemens AG, Munich, Germany) processed around 10%. We analyzed the change in the TAT for the CBC for over 7 years, from January of 2005 to December of 2011. The delta check made alterations of delta to WBC, hemoglobin, hematocrit, platelet and metamyelocyte, however, did not made them to band neutrophil, eosinophil, basophil and monocyte. The panic value check made alterations of panic value to hemoglobin, hematocrit, platelet and monocyte. In the criteria of currently slide review, LH-780 and ADVI-2120 analyzers prepared suspect flags of "Blast, Imm NE2, Immature granulocyte, Imm NE1, Left shift, Variant lymphocyte, Atypical lymphocyte, Platelet clumps and NRBC". The New slide review in the XE-2100 analyzer altered the preparations of a smear slide more than a "Platelet clumps flag(${\geq}200unit$), a single flag excluding the "Platelet clumps flag (${\geq}250unit$) and a multiple flag (${\geq}200unit$)". Also, below the 240 unit, medical technologists prepared manual slides selectively according to their evaluations. The automatic reporting rate was 33.4% without alterations, whereas it was 41.0% without alterations, and was thus improved by 7.6%. The slide review rate was 15.2% before using the Q-flag limit, whereas it was 12.1% for a reduce 3.1%. TAT was 45 minutes without the creation alterations of the delta and panic value checks, whereas it was 35 minutes after making alterations of the delta and panic value checks and thus was shortened by 10 minutes. We came to the conclusion that the establishment and operation of delta and panic value checks and slide review criteria suitable for laboratory environment can reduce unnecessary smear slides, re-checking, re-sampling, re-testing, telephone inquiries and concentrated workloads during specific times of the day.

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분광광도계를 이용한 720 nm에서 망상적혈구 측정 경험 (Experience of Reticulocytes Measurement at 720 nm Using Spectrophotometer)

  • 성현호;석동인;정유현;김대중;이석재
    • 대한임상검사과학회지
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    • 제49권4호
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    • pp.382-389
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    • 2017
  • 임상 실험실에서의 망상 적혈구 계산은 현재 수동 및 자동으로 구분된다. 현미경에 의한 망상 적혈구 수 검사는 정확성의 결여, 낮은 재현성 등이 망상 적혈구 결과의 정확성에 문제가 있다. 또한, 자동 혈액 분석기의 유동 세포 계측법은 개발 도상국 및 소규모 실험실에서 비용 때문에 사용하기가 어렵다. 따라서 본 연구는 이러한 단점을 보완 할 수 있는 새로운 방법을 찾기 위해 노력했다. 이 연구의 목적은 분광 광도계로 염색 된 망상 적혈구 수를 비교하고 또한 분광 광도계 및 유동 세포 계측기의 통계를 분석하는 것이다. 동일한 8 개의 EDTA 샘플을 36 회 반복하여 분광 광도계와 유동 세포 계측기 사이의 일치도를 비교 하였다. 이 연구는 700~780 nm에서 600 배 희석 한 표본을 10 개의 차이로 측정하였다. 흡광도에 의한 710~730 파장은 표준 및 검사와 양의 상관 관계가 있으며(r=0.967, p<0.01), 변수에 대한 상관 관계를 나타내었다. 결과에 대한 회귀 분석결과 최적 희석 계수는 600 배였다. 따라서 본 연구는 자동 분광 광도계를 이용한 망상 적혈구 흡광도 측정에 대한 경제적인 기여 그리고 망상 적혈구 관련 빈혈에 대한 모니터링 시스템 등에 기여 할 수 있는 기술적 활용을 시도한 것이다. 따라서 더욱 광범위한 연구가 필요할 것으로 사료된다.

Isolation and Identification of Staphylococcus sp. from Korean Fermented Fish Products

  • Um, Mi-Na;Lee, Cherl-Ho
    • Journal of Microbiology and Biotechnology
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    • 제6권5호
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    • pp.340-346
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    • 1996
  • In order to find out if staphylococci occur in significant numbers in Korean fermented fish products, a total of 40 different fermented fish products were collected from different markets in Korea and analyzed for their physico-chemical and microbiological states. The pH, salt concentration and water activity of the products were measured and the total viable cell count and the number of Staphylococcus grown on mannitol salt agar were determined. The identification of the strains of Staphylococcus were made by API Staph Strip and MIS identification kits, and the physiological properties of the identified strains were further characterized by different conventional methods. The pH, salt content and water activity of fermented fish samples varied widely from 4.8 to 7.1, 7.4-28.7$%$ and 0.77-0.84, repectively, depending on the type of product. The total viable cell count varied from $10^4-10^9$ cfu/ml, and most of the samples had $10^5-10^6$ cfu/ml No correlation was found between the viable cell count and the pH, NaCl concentration and water activity of the samples. Among the 35 colonies identified as Staphylococcus strains by the identification kits, S. xylosus was the most frequently occurring strain marking 17, and S. warneri was 8, S. epidermidis 4 and S. cohnii 2. S. hominis, S. saprophyticus, S. haemolyticus and S. aureus were also identified once each. In some samples (K-3, P-6, K-8, G-5 and G-10), 2-3 different species of Staphylococcus were found. Considering the region of sampling, among the 10 samples from Kunsan 5 were identified as S. warneri, while in the other regions S. xylosus was predominant. Although the physiological characteristics of the identified strains were generally consistent with those in Bergey's Manual, some discrepances were also observed. All the strains were highly salt tolerant, growing in the media containing over 18$%$ NaCl. All the strains except S. aureus (G-11) showed negative in hemolysis activity, plasma coagulation and DNase tests. All the strains including S. aureus (G-11) showed negative in enterotoxin test.

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생물학적 영상 분석을 위한 자동 모바일 셀 계수 시스템 (An Automatic Mobile Cell Counting System for the Analysis of Biological Image)

  • 서재준;전준철;이진성
    • 인터넷정보학회논문지
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    • 제16권1호
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    • pp.39-46
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    • 2015
  • 본 논문에서는 모바일 환경에서 미세세포 영상으로부터 셀을 자동 검출하고 계수하는 자동화 방법을 제시하였다. 셀 카운팅은 생물학 또는 병리학적 영상분석에 있어서 매우 중요한 과정이다. 과거에는 셀 카운팅은 수동적인 방법으로 진행되어 매우 지루하고 많은 시간을 필요로 하는 작업이었다. 이에 더하여 수동 계수 방법은 정확한 카운팅 결과를 도출하는데 어려움이 있었다. 따라서, 정확하고 일관된 셀 검출과 카운팅 결과를 생물학적인 영상으로부터 획득하기 위해서는 자동화방법이 필요하다. 제안된 다단계 셀 계수방법은 배양된 세포영상으로부터 셀을 자동으로 분할하고 분할된 셀의 위상학적 분석을 통하여 셀을 라벨링 한다. 셀 카운팅의 정확도를 높이기 위하여 워터쉐드 알고리듬에 의하여 서로 덩어리로 뭉쳐진 셀을 서로 분리하고 모폴로지 연산을 통하여 영상으로부터 획득한 개별 셀의 형태를 개선한다. 제안된 시스템은 모바일 환경에서 사용될 수 있도록 개발되었다. 따라서 셀 영상은 모바일 폰의 카메라로 획득하며 미세세포의 통계학적인 분석 데이터는 유비쿼터스 환경의 모바일 장치에 의해 전송 된다. 실험을 통하여 수동으로 계수한 셀의 숫자와 제안된 방법에 의해 자동 카운팅 된 셀의 수를 비교한 결과 제안된 방법이 매우 효과적이고 정확한 결과를 제시한다는 사실을 입증하였다.

자바 프로그래밍에서 병렬처리를 위한 중첩 루프 구조의 다중스레드 변환 (Transform Nested Loops into MultiThread in Java Programming Language for Parallel Processing)

  • 황득영;최영근
    • 한국정보처리학회논문지
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    • 제5권8호
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    • pp.1997-2012
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    • 1998
  • 병렬 시스템에서 순차 자바 프로그램을 재 사용할 수 있기 위해서는 자바 프로그램 내에 존재하는 병렬성을 찾아내는 것이 중요하다. 자바 프로그램을 병렬 시스템에서 실행할 경우 루프는 전체 수행 시간 중 많은 부분을 차지하므로 병렬성 검출의 기본이 되지만 데이터 종속으로 인하여 완전한 병렬 수행을 쉽게 이룰 수 없다. 따라서, 본 논문은 기존의 중첩 루프 구조를 갖는 자바 프로그래밍에서 데이터 종속성 분석에 의한 종속 그래프를 구성하여 묵시적 병렬성을 검출하는 방법을 제안한다. 또한 재구성 컴파일러에 의하여 자바 원시 프로그램을 자바 프로그래밍 언어 자체에서 지원하는 다중스레드 기법으로 변환하여 병렬 시스템에서 실행하는 방법을 제안한다. 스레드 문장으로 변환된 프로그램에 대해 루프의 반복계수와 스레드 수를 매개변수로 하여 성능 분석을 하였다. 재구성 컴파일러에 의한 장점은 사용자의 병렬성 검출에 대한 오버해드를 줄이고, 순차 자바 프로그램에 대한 효과적인 병렬성 검출을 가능하게 하여 병렬 시스템에서 실행 시간을 단축할 수 있다.

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SPECT/CT에서 인공고관절에 대한 감쇠보정지도(Attenuation Map)의 매개변수 변화에 따른 금속 인공물(Metal Artifact)의 영향 평가 (Evaluation of the Effect of Metal Artifacts Varying the Parameters of the Attenuation Map for the artificial Hip Joint in SPECT/CT)

  • 김상규;김정열;박민수;조승현;임한상;김재삼
    • 핵의학기술
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    • 제18권2호
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    • pp.3-7
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    • 2014
  • CT를 기반으로 감쇠보정이 시행되는 SPECT/CT 검사에서는 금속 삽입물에 의한 선속경화현상으로 인접한 부위의 과대평가를 유발하며, 영상의 질을 저하시킨다. 이에 대해, 본 연구에서는 인공고관절이 삽입된 팬텀을 이용하여 감쇠보정지도의 매개변수 변화에 따른 금속 인공물이 SPECT/CT 영상에 미치는 영향을 알아보고자 한다. SPECT/CT 장비는 Siemens사의 Symbia T16을 사용하였다. SPECT/PET 팬텀에 인공고관절을 삽입하고, CT영상에서 Bright Streak 영역에 직경 17 mm의 구를 배후방사능 대비 8배가 되도록 Tc-99m을 채웠다. 이후 감쇠보정지도에서 Wide Beam Coefficient[수동모드(0.1~0.9), 자동모드]변화에 따른 열소와 배후방사능의 계수를 측정하였고, 고관절의 유무에 따라 Metal과 Non-Metal의 병소 대 배후방사능 비(Region to Background Ratio, RBR)를 산출 및 비교 분석하였다. 수동모드의 값이 증가할수록 Metal과 Non-Metal의 열소 계수는 증가하는 경향을 나타냈으며, 수동모드 0.4와 0.5에서 두 군과의 열소 계수비가 가장 일치하게 나타났다(수동모드 0.4=1.001, 0.5=0.999). 또한 $RBR^{Metal}$$RBR^{Non-Metal}$의 비는 자동모드에서 1.135이였고, 수동모드 0.4와 0.5에서 통계적으로 유의한 차이를 보이지 않았다(0.4=0.999, 0.5=1.028, p=0.78). 감쇠보정지도에서 Wide Beam Coefficient의 자동모드는 13.52% 과 보정 되는 것을 알 수 있었으며, 수동모드 중 0.4와 0.5에서 인공물에 의한 과 보정을 최소화 할 수 있었다. 이를 활용하여 환자에게 적용하기 위해서는 추가적인 연구가 이루어져야 할 것이며, 고관절전치환술 환자의 인공고관절 주변에서 금속 인공물에 의한 과 보정을 감소시켜 진단능을 향상시킬 수 있을 것으로 사료된다.

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