• Title/Summary/Keyword: Male sterile line

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Testing Bacterial Spot Resistant Lines of Capsicum Pepper for Nuclear Genotype Interacting with Male Sterile Cytoplasm (고추 더뎅이병 저항성 계통의 세포질웅성불임 관련 핵내유전자형 검정)

  • 김병수;황희숙
    • Korean Journal Plant Pathology
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    • v.14 no.3
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    • pp.212-216
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    • 1998
  • Capsicum pepper selections fro PI163192, PI241670, PI244670, PI271322, PI308787, PI322719, and PI369994 were confirmed to be non-hypersensitively resistant to race 3 of Xanthomonas campestris pv. vesicatoria. A resistant cultivar,‘SR’, was shown to be hypersensitive. Four Koran local cultivars, a cytoplasmic male sterile line (A-line) and its maintainer (B-line) were highly susceptible. The resistant selections and cultivars were crossed with a male sterile A-line (Smsms) and fertility of their F1 hybrids was examined by observing he pollen production, testing pollen germination, and quantifying the amount of pollen produced per anther to identify the genotype interacting with the male sterile cytoplasm. The seven resistant PI selections turned out to be restorers (N(S)MsMs) and‘SR’to be a maintainer (Nmsms).

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Early Maturing Male Sterile Line of Onion (Allium cepa L.) 'Wonye 30002' (양파 조생계 웅성불임 중간모본 '원예 30002')

  • Kim, Cheol-Woo;Lee, Eul-Tai;Choi, In-Hu;Jang, Young-Seok;Suh, Sae-Jung
    • Korean Journal of Breeding Science
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    • v.42 no.3
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    • pp.298-301
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    • 2010
  • A new intermediate parent 'Wonye 30002' (Allium cepa L.) was developed by Bioenergy Crop Research Center, NICS in 2009. As a male sterile line, 'Wonye 30002' can be used to hybrid seed production by crossing with pollen parent. The first cross was conducted between male sterile plants of 402AC203 and M1 in 2002. The male sterile line 'Wonye 30002' has circular bulb and bulb weight is 283 g. As early maturing type, lodging date is May 6. Plant height and pseudostem diameter are 43 cm and 15.5 mm, respectively. In seed harvesting characteristics, number of flower stalks and the length are 43 cm and 110 cm, respectively. The flowering date of 'Wonye 30002' is around May 24 and is completely male sterile. 'Wonye 30002' is a promising male sterile line for hybrid bulb onion seed production.

Mid-late Maturing Male Sterile Line of Onion (Allium cepa L.) 'Wonye 30001' (양파 중생계 웅성불임 중간모본 '원예 30001')

  • Kim, Cheol-Woo;Lee, Eul-Tai;Choi, In-Hu;Jang, Young-Seok;Suh, Sae-Jung
    • Korean Journal of Breeding Science
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    • v.42 no.3
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    • pp.294-297
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    • 2010
  • A new intermediate parent 'Wonye 30001' (Allium cepa L.) was developed by Bioenergy Crop Research Center, NICS in 2009. As a male sterile line, 'Wonye 30001' could be used for hybrid seed production by crossing with pollen parent. The first cross was conducted in 2000. Male sterile plants were selected from 'Ginque' breeding lines and fertile plants were selected from YG-1-1. The male sterile line 'Wonye 30001' has circular bulb and bulb weight of 260 g. As mid-late maturing type, lodging date is around May 20. Plant height and stem diameter are 57 cm and 15.9 mm, respectively. In seed harvesting characteristics, number of flower stalks and the length are 6 and 135cm, respectively. Flowering date of 'Wonye' is May 23 and is completely male sterile. 'Wonye 30001' is a promising male sterile line for hybrid bulb onion seed production.

Selection in $F_3$ and $F_4$ Generations of a Cross for Breeding Genic Male Sterile Pepper Lines Resistant to Phytophthora Blight and Bacterial Spot (역병과 세균성점무늬병에 복합저항성인 핵유전형 웅성불임성 고추 계통 육성을 위한 교배의 $F_3-F_4$세대 선발)

  • Park, Dong-Keun;Kim, Byung-Soo
    • Current Research on Agriculture and Life Sciences
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    • v.28
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    • pp.17-23
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    • 2010
  • Selection procedures for breeding genic male sterile lines for resistance to both Phytophthora blight caused by Phytophthora capsici and bacterial spot caused by Xanthomonas euvesicatoria were executed to $F_3$ and $F_4$ generations derived from a cross between a Phytophthora resistant genic male sterile (GMS) breeding line and a bacterial spot and Phytophthora resistant breeding line. Resistance to P. capsici was originally introduced from KC294(CM334) and KC263(AC2258), the well-known sources of resistance to P. capsici. Resistance to bacterial spot was introduced from KC47(PI244670). GMS lines with high resistance to P. capsici were obtained and the selected lines are expected to be quantitatively resistant also to bacterial spot.

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Comparison in Anthesis Characterics of Some Male Sterile Rice Cultivars (수도 웅성불임계통의 개화특성 비교)

  • Suh, Hak-Soo;Cho, Young-Chan;Park, Tae-Seok
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.30 no.4
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    • pp.427-430
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    • 1985
  • Extrusion degree of stigma, numbers of opened florets at different time during anthesis, angle of opening florets and outcrossed seed set percent were investigated in four male sterile rices having background of Korean cultivars; Suwon 296A, Suwon 304A, Suwon 310A and Suwon 296GMS, and the Chinese cytoplasmic genetic male sterile rice Zhenshan 97A as control in order to get basic informations for hybrid rice breeding. The results obtained were summarized as follows; Extrusion degree of stigma in the four Korean male sterile lines tested was lower than in the Chinese one Zhenshan 97A. The better extrusion of stigma showed the higher outcross rate in male sterile rices. There were almost no relationships between peak of anthesis and outcrossed seed set in the male sterile rices tested. Large anthesis angle with long anthesis duration appeared to be essential for high outcrossed seed set of the male sterile rices. The genetic male sterile line showed the larger anthesis angle than the cytoplasmic and genetic male sterile one in the lines having the same background to the Korean rice cultivar Suwon 296.

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Breeding Hybrid Rice with Good Quality and High Yield I. Breeding of Cytoplasmic-Genetic Male Sterile and Restorer Lines with Backgrounds of Korean Rice Varieties (양질 다수성 일대잡종 벼 육성 연구 I. 우리나라 품종 배경의 세포질-유전자적 웅성불임 및 임성회복 계통 육성)

  • Hak-Soo SUH
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.5
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    • pp.413-417
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    • 1993
  • This study was conducted to breed various cytoplasmic genetic male sterile (CGMS) and restorer lines with backgrounds of Korean japonica rice varieties. The CGMS line BT-CMS was crossed with the restorer line AR-3. The fertile $F_1 was emasculated and crossed with the Korean japonica rice varieties of the early maturing Sobaegbyeo, Odaebyeo, Gwanagbyeo and Daeseongbyeo, and of the medium maturing Hwajinbyeo, Paldal, Suwon 224 and Iri 386, and of the late maturing Nagdongbyeo, Palkweng, Hwacheongbyeo and Milyang 97. Each of the three way cross $F_{1S} was segregated into fertile and sterile individuals. The sterile individuals in each cross were discarded and the fertile individuals were emasculated and backcrossed with the Korean japonica rice varieties. The same process was applied from BC$_1$F$_1$ to BC$_4$F$_1$ generation. In the ${BC_4}{F_1}of each cross, the male sterile individual was crossed with the recurrent Korean japonica variety which was maintainer of male sterility. The male sterile lines of ${BC_6}{F_1}were named as Sobaegbyeo R, Odaebyeo R, Gwanagbyeo R, etc. The fertile individuals homozygous in pollen fertility were selected from the ${BC_4}{F_2}generation and named as Sobaegbyeo R, Odaebyeo R, Gwanagbyeo R, etc. Agronomic characteristics of the CGMS, restorer lines with backgrounds of Korean japonica, and the recurrent Korean japonica rice varieties grown in the field condition were compared. Culm length of the CGMS lines tended to be shorter than that of the recurrent parent, however no significant differences in heading date, panicle length and yield component were found among the CGMS, restorer and recurrent lines.

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Restorer Genotype for Male Sterile Cytoplasm of Genetic Resources Moderately Resistant to Phytophthora capsici in Capsicum Pepper

  • Kim, Byung-Soo;Ahn, Joon-Hyung;Lee, Jae-Moo;Park, Dong-Guen;Kim, Hye-Yeon
    • Horticultural Science & Technology
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    • v.30 no.1
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    • pp.73-78
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    • 2012
  • KC00256, KC00406, KC00462, KC00463, KC00820, and KC00821, the genetic resources that have previously been reported as moderately resistant to Phytophthora capsici, as well as the line KC01322, a new source of moderate resistance introduced from Laos, were tested against two strains (Pc003 and Pc005) of P. capsici. We also determined the nuclear restorer genotypes of these lines, in regards to their interaction with cytoplasmic male sterility, through crossing the resources with cytoplasmic male sterile Punggok-A (Srfrf) and determining the fertility of the $F_1$ hybrids. The studied lines exhibited a low level of resistance to both the strains of P. capsici compared to highly resistant CM334, but their response was fairly consistent for both P. capsici strains. KC00406, KC00462, KC00463, and KC01322 produced stable, male fertile $F_1$ plants indicating that they are restorers with genotype N(S)RfRf. KC00821 produced male sterile $F_1$ plants and was identified as a maintainer with genotype Nrfrf. The $F_1$ plants of the KC00820 cross, however, set a few male fertile flowers in the greenhouse at seedling stage, then became male sterile after being transplanted to the plastic greenhouse soil in May and remained so to the end of the growing season. Therefore, KC00820 is an unstable maintainer with genotype Nrfrf. The moderate resistance exhibited by these genetic resources may be integrated into breeding programs aimed at promoting higher levels resistance via recurrent selection or hybridization.

Breeding Cytoplasmic Male Sterile Lines Resistant to Phytophthora capsici and Ralstonia solanacearum in Capsicum Pepper (역병-풋마름병 복합저항성 세포질웅성불임계 고추 육성)

  • Lee, Jae-Moo;Kim, Byung-Soo
    • Current Research on Agriculture and Life Sciences
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    • v.28
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    • pp.39-46
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    • 2010
  • Selection procedures for breeding lines resistant to both bacterial wilt caused by Ralstonia solanacearum and Phytophthora blight caused by P. capsici were executed to generations from $F_2$ and $BC_1F_1$ to $F_4$ and $BC_1F_3$ of crosses between a Phytophthora resistant breed, 'Chilbok No. 1' and bacterial wilt resistant accessions introduced from Vietnam during 2009 and 2010. The breeding populations or lines were tested for resistance to P. capsici and resistant plants were selected. The resistant selections were inoculated with R. solanacearum to discard susceptible plants. Resistance to P. capsici was conspicuously improved by selection from $F_2$ and $BC_1F_1$ and the final selections showed a similar level of resistance to P. capsici as a commercial Phytophthora resistant cultivar, Muhanjilju. A few $BC_1F_2$ selections were crossed to a cytoplasmic male sterile line, Chilbok-A, to identify their nuclear genotype interacting with male sterile cytoplasm. Majority of them was fixed to maintainer (Nrfrf) and only two resulted segregating into male sterile and male fertile plants indicating that the pollen parents were heterozygous in the fertility-restoring gene.

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Studies on the Heterosis Breeding in Rape by Using Cytoplasmic Genetic Male Sterile lines 3. Development of Complete Cytoplasmic Genetic Male Sterile Line "MOKPO-MS" Having Improved Quality of Oil and Oil Cake (세포질.유전자적 웅성불임 계통을 이용한 유채 Heterosis 육종에 관한 연구 제3보 유채 세포질 유전자적 웅성불임 계통의 불임안전성과 유.박의 성분 개량)

  • Lee, J.I.;Kwon, B.S.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.25 no.3
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    • pp.50-58
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    • 1980
  • A cytoplasmic-genetic male sterile line(MS) in rapes of which fertility is not restored under any temperature regimes and have the better quaility of and oil cake was developed. A MS line named Mokpo-MS, which is form the cross between Tower and Isuzu and its stamens is degenerated completely by interact~g with cytoplasmic and nuclear genes was selected. This MS line was found as a complete cytoplasmic-genetic male sterile having the quality of oil and oil cake were greatly improved by introducing Zero-erucic and Zero-glucosinolate gene from Tower.

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Breeding for Potato Virus Y Resistant Male-sterile $F_1$ Hybrid KB 109 in Nicotiana tabacum L. (연초의 감자바이러스 Y 저항성 웅성불임 일대잡종 KB 109 육성)

  • 조천준;김대송;정석훈;최상주;조명조
    • Journal of the Korean Society of Tobacco Science
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    • v.16 no.2
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    • pp.134-138
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    • 1994
  • Potato Virus Y(PVY), vein necrosis strain, in Korea causes severe symptoms on burley tobacco(Nicotiana tabacum L.). As the results, programs to incorporate PVY resistance into commercial cultivars were initiatEd. But the development of the homozygous fertile line resistant to PVY is time consumming. This study was conducted whether the Fl hybrid could be used to reduce the yield losses caused by PVY. Four F1 hybrids were made between male - sterile(ms) NC 107 and KB 107 as maternal parent, and TC 612 and TC 613 as Pollen donor, respectively, and were evaluated for their PVY resistance and negatively associated traits. (ms NC 107 X TC 612) F1, named as KB 109, Ivas applied to yield trial and compared with commercial cultivars for the level of disease resistance, agronomic characteristics, chemical contents and physical properties. All Fl hybrid could be used commercially as the PVY resistant cultivar. Especially KB 109 have the resistance against PVY, tobacco mosaic virus and black shank(Phytophthora parasitica var. nicotianae). It had wider leaves, flowered one day later, and yield of acceptable quality was higher than that of Burley 21, standard cultivar in Korea.

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