• Title/Summary/Keyword: Magnetic Resonance Spectroscopy

Search Result 628, Processing Time 0.03 seconds

High-pressure NMR analysis on Escherichia coli IscU

  • Jongbum Na;Jinbeom Si;Jin Hae Kim
    • Journal of the Korean Magnetic Resonance Society
    • /
    • v.28 no.1
    • /
    • pp.1-5
    • /
    • 2024

  • IscU, the iron-sulfur (Fe-S) cluster scaffold protein, is an essential protein for biogenesis of Fe-S clusters. Previous studies showed that IscU manifests a metamorphic structural feature; at least two structural states, namely the structured state (S-state) and the disordered state (D-state), interconverting in a physiological condition, was observed. Moreover, subsequent studies demonstrated that the metamorphic flexibility of IscU is important for its Fe-S cluster assembly activity as well as for an efficient interaction with various partner proteins. Although solution nuclear magnetic resonance (NMR) spectroscopy has been a useful tool to investigate this protein, the detailed molecular mechanism that sustains the structural heterogeneity of IscU is still unclear. To tackle this issue, we applied a high-pressure NMR (HP-NMR) technique to the IscU variant, IscU(I8K), which shows an increased population of the S-state. We found that the equilibrium between the S- and D-state was significantly perturbed by pressure application, and the specific regions of IscU exhibited more sensitivity to pressure than the other regions. Our results provide novel insights to appreciate the dynamic behaviors of IscU and the related versatile functionality.

  • https://doi.org/10.6564/JKMRS.2024.28.1.001 인용 PDF

Deuterium NMR Studies of $({\mu}_3-C^2H)[Co(CO)_3]_3$

  • Woo, Ae-Ja;Maria I. Altbach;Leslie G. Butler
    • Journal of the Korean Magnetic Resonance Society
    • /
    • v.1 no.2
    • /
    • pp.95-102
    • /
    • 1997

  • Deuterium quadrupole coupling constant (e2qzzQ/h) of ($\mu$3-C2H)[Co(CO)3]3 was determined by using solid-state deuterium MAS NMR spectroscopy. The small quadrupole coupling constant of bridging methyne unit relative to sp-acethylene in propyne is discussed in terms of the C-H bond length and the negative charge on the carbon.

  • PDF

Solution and Solid-state Vanadium-51 NMR Studies of Vanadium (V) Complexes

  • Lee, Man-Ho
    • Journal of the Korean Magnetic Resonance Society
    • /
    • v.1 no.1
    • /
    • pp.1-6
    • /
    • 1997

  • Several dioxovanadate (V) complexes are synthesized and studied by solution and solid-state 51V NMR spectroscopy. In the results, large 51V chemical shift anisotropy ({{{{ DELTA delta }}a = -800 ∼720 ppm) and quadrupole coupling (e2q /h = 7.50 ∼ 9.16 MHz) were observed in the solid-state complexes. The isotropic chemical shifts of the solid samples are very close to the values obtained from solution measurements.

  • PDF

Characterization of the Catalytic Heteropoly Compounds using Solid-state NMR

  • Kim, Y;Lee, W
    • Journal of the Korean Magnetic Resonance Society
    • /
    • v.1 no.1
    • /
    • pp.45-58
    • /
    • 1997

  • Heteropoly compounds, H3PMo12O40, CsxH3-xPMo12O40, and vanadium containing heteropoly compound were characterized by Solid-state broad line 1H MAS NMR, 31P MAS NMR, and High Speed MAS 51V NMR spectroscopy of quadrupolar nuclei. The effects of calcination, dehydration, and the number of protons on the structure of heteropoly compounds were studied. The results of this study demonstrate that these Solid-state NMR techniques are very useful tools to study heteropoly compounds.

  • PDF