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Determination of Optimum Rice Harvest Time by Change of the Glutelin Contents During the Maturity Period (벼 등숙기간 중 글루테린 함량 변화에 따른 쌀의 수확적기 판정)

  • Shin, Pyung-Gyun;Chang, An-Cheol;Hong, Seong-Chang;Lee, Ki-Sang;Lee, Keum-Hee;Lee, Yong-Bok
    • Korean Journal of Environmental Agriculture
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    • v.27 no.4
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    • pp.460-463
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    • 2008
  • The change of glutelin contents in rice grain during the maturity period was investigated to determine optimum rice harvesting time. The glutelin content was increased with increasing time after heading. In this study, eight of glutelin subunits were found. Among the glutelin subunits, 7208-subunit (MW, 35 kD) contents was significantly increased at 65 days after heading compared with 55 and 60 days after heading. 7405-subunit (MW, 50 kD) contents was steadily increased with time after heading. The results showed that at 55th day after heading would be optimum time for harvest to get the low glutelin content of rice grain.

MPEG-A PART 9 DIGITAL MULTIMEDIA BROADCASTING APPLICATION FORMAT

  • Sabirin, Muhammad Syah Houari;Lee, Jung-Soo;Kim, Hui-Yong;Kim, Mun-Churl;Kim, Yong-Han
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2009.01a
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    • pp.346-350
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    • 2009
  • Digital Multimedia Broadcasting (DMB) is the mobile TV service based on a digital radio transmission system that provides high quality audio/video and other auxiliary data services. As users want to store the DMB content in their device to be consumed later or to be shared among users, a standardized format needs to be specified to guarantee the interoperability for the DMB contents for various devices. DMB AF (Application Format) specification defines a file format for DMB contents and services. It specifies how to combine the variety of DMB contents with associated information for a presentation in a well-defined format that facilitates storage, interchange, management, editing, and presentation of the DMB contents in protected, governed, and interoperable ways. In this paper we present our implementation of DMB AF as part of the development of DMB AF reference software. Our implementation of DMB AF is developed as the reference software for the standard specification that consists of a three applications: packager, media player, metadata browser and collection of supporting libraries used by the applications.

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Changes of Rice Storage Proteins Affected by Dry and Storage Temperature (건조 및 저장조건에 따른 쌀 저장단백질의 변화양상)

  • Shin, Pyung-Gyun;Chang, An-Cheol;Hong, Seong-Chang;Lee, Ki-Sang;Lee, Keum-Hee;Lee, Yong-Bok
    • Korean Journal of Environmental Agriculture
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    • v.27 no.4
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    • pp.456-459
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    • 2008
  • Quality of rice grain changes during dry storage with internal physiological changes and external injury by organism. Storage rice changes by condition with respiration via variable temperature, hydrolysis enzyme reaction, lipid peroxidation occurs with change of palatability. During dry storage, physiological change with protein variation pattern was examined by image analysis on proteomic technology. Analysis revealed that protein activity had no change store at room temperature and store at $40^{\circ}C$, but decreased store at $60^{\circ}C$. Analysis of variable hydrophobic protein pattern revealed that protein activity of beta-tubulin, protein disulfide isomerase, vacuolar ATPase b subunit, globulin was not significantly decreased all dry and store condition. However, heat shock protein 70, and glutathione transferase was significantly decreased when rice dried at $60^{\circ}C$ compared with room temperature and $40^{\circ}C$ dry condition.

Induction of twinning in Korean native cattle by transfer of nuclear transplanted embryos I. Embryo cloning using ovum pick-up(OPU), electric cell fusion and in vitro culture system (핵이식 기법을 이용한 한우 쌍태생산에 관한 연구 I. Ovum pick-up(OPU), 전기적 세포융합 및 체외배양 기법을 이용한 복제수정란 생산)

  • Hwang, Woo-suk;Shin, Tae-young;Roh, Sang-ho;Lee, Byeong-chun
    • Korean Journal of Veterinary Research
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    • v.38 no.1
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    • pp.209-216
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    • 1998
  • The purpose of present study is to improve the efficiency of fusion and the developmental rates of nuclear transplanted embryos to produce genetically identical twins from Korean native cattle. The diameter of aspirated follicles had no significant effect on the recovery rates of oocytes collected by ovum pick-up technique. The fusion rates of nuclear transplanted embryos were significantly higher in 50 and $100{\mu}s$ DC duration groups(73.3 and 72.0% ; respectively) than that in $30{\mu}s$ group(55.6% ; p<0.05). The cleavage rates of nuclear transplanted embryos appeared to be significantly higher in donor nuclei derived from in vivo (65.0%) than in those from in vitro (50.5% ; p<0.01), but the developmental rates to morulae and blastocysts were not significantly different between them(13.7 vs 10.9%, respectively).

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The integration of genomics approaches for lettuce (Lactuca sativa L.) improvements on the disease resistances and other agronomic qualities.

  • Kim, Tae-Sung;Kim, Jeong-Haw;Kim, Jung-Bun;Jang, Suk-Woo
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.114-114
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    • 2017
  • The aim of this research is to improve Korean lettuce varieties in terms of Fusarium wilt, bolting under hot weather and nutritional function applying genomics approaches. To find related gene/molecular markers, we selected 96 lettuce varieties which are popular in domestic fresh vegetable markets. To construct frame works of the genomic approaches, we exploited GBS(Genotyping by Sequencing) and found total 61,407 SNPs from lettuce whole genomes (MAF>0.02). We observed that Three SNPs array per 100kb of lettuce genome. Average LD decay is expected to expand up to 3.9M(million)bp. Thus, we concluded that about 104 SNPs exist within a LD, which is sufficient to use GWAS(Genome-wide Association Study) to explore the useful gene/molecular markers. In addition, we optimized mass screening method to evaluate disease resistance levels against Fusarium wilt and are testing the bolting sensitivity during summer growing season for those lettuce allele mining set.

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Characteristics of Ampicillin-Resistant Vibrio spp. Isolated from a West Coastal Area of Korean Peninsula (서해안에서 분리한 암피실린 내성 비브리오속 세균의 특성)

  • Lee, Han-Woong;Lim, Suk-Kyung;Kim, Mal-Nam
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.1
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    • pp.20-25
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    • 2009
  • Thirty-eight Vibrio spp. were isolated from the sea waters harvested from the 22 stations located on the west coast of the Korean peninsula in September 2006. The isolates consisted of V. parahaemolyticus (n=21), V. alginolyticus (n= 16) and V. cholerae non-01 (n=1), among which 35 isolates displayed resistance against two of the tested antibiotics. Among the 38 isolates, 18 isolates exhibited multi-drug resistance against more than four 4 antibiotics. In particular, minimum inhibitory concentration $(MIC)_{50}$ and $MIC_{90}$ of ampicillin-resistant isolates were as high as $2,048{\mu}g{\cdot}mL^{-1}$ and $4,096{\mu}g{\cdot}mL^{-1}$ respectively. $\beta$-lactamase production was examined to analyze the ampicillin-resistance. Some Vibrio spp. isolates produced $\beta$-lactamase, however antibiotics resistance pattern and $\beta$-lactamase production were not clearly related to each other. A genetic relationship between resistance and gene expression was confirmed in the ampicillin-resistant isolates.

Analysis of Genetic Diversity and Relationships of Korean Native Black Goat using Microsatellite Markers (초위성체 마커를 이용한 한국 재래 흑염소의 유전적 다양성 및 유연관계 분석)

  • Park, Byeong Kyu;Kim, Yi Seul;Seong, Jiyeon;Kong, Hong Sik
    • Journal of Animal Reproduction and Biotechnology
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    • v.34 no.3
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    • pp.183-189
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    • 2019
  • The aim of this study was to assess the levels of genetic diversity and relationships of Korean native black goat (n = 58), compared with the exotic breed, Boer (n = 97). For the analysis of genetic characterization 11 microsatellite markers (MAF065, INRA063, CSRD247, OarFCB20, SRCRSP5, INRA006, ILSTS008, ILSTS011, INRA005, ILSTS087, SRCRSP8) were genotyped. The number of alleles was observed 3 (INRA005) to 10 (SRCRSP8) each markers. The mean expected and observed heterozygosity (Hexp and Hobs) and polymorphism information content (PIC) for the Korean native black goat breed varied from 0.551 to 0.860, 0.517 to 0.948 and 0.464 to 0.835, respectively. Principal Components Analysis (PCoA) and FCA results showed that Korean native black goat breed was confirmed to be clearly separated from bore breed. These results were scientific evidence that Korean native black goat represents a unique and valuable animal genetic resource.

Comparison of Genetic Diversity among Amaranth Accessions from South and Southeast Asia using SSR Markers (SSR 마커를 이용한 남아시아와 동남아시아 아마란스 자원의 유전적 다양성 비교)

  • Wang, Xiao Qiang;Park, Yong Jin
    • Korean Journal of Medicinal Crop Science
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    • v.21 no.3
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    • pp.220-228
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    • 2013
  • This study was conducted to assess the genetic diversity and population structure of 70 amaranth accessions collected from South and Southeast Asia using 14 simple sequence repeat (SSR) markers. In total, 67 alleles were detected, with an average of 4.79 per locus. Rare alleles comprised a large portion (46.3%) of the detected alleles, and 29 unique alleles associated with rice accessions were also discovered. The mean major allele frequency (MAF), genetic diversity (GD) and polymorphic information content (PIC) of the 14 SSR loci were 0.77, 0.36, and 0.34, respectively. A model-based structural analysis revealed the presence of three subpopulations. The genetic relationships revealed by the neighbor-joining tree method were fairly consistent with the structure-based membership assignments for most of the accessions. All 70 accessions showed a clear relationship to each cluster without any admixtures. We observed a relatively low extent of genetic exchange within or among amaranth species from South and Southeast Asia. The genetic diversity results could be used to identify amaranth germplasms and so facilitate their use for crop improvement.

A Study on the Quality Safety of Apples by Region Using MA Packaging Materials (MA포장재 이용한 지역별 사과의 품질 안전성에 관한 연구)

  • Hong, Sang Tai
    • Journal of the Korea Safety Management & Science
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    • v.22 no.2
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    • pp.1-6
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    • 2020
  • Apples are one of the most produced fruits in Korea, with 2,423,000 tons of fruits produced in Korea in 2018, of which 365,000 tons (MAF, 2018) account for about 15% of the total production. For quality safety after harvesting apples, the application of MA(modified atmosphere) packaging technology (Mostofi et. al., 2008) is being actively studied. In addition, the effects of functional packaging materials that have added functions such as fireproof, antibacterial, high-blocking, degradability, and far-infrared radiation have been studied (Chung et al., 2009). In addition, there are reports of the effects of quality changes (Park et al., 2007) and MA storage methods on the quality of apples by packing apples with functional MA films((Park et al., 2004). But there are only few reports on quality safety by analyzing the correlation between the change of reducing sugar and total sugar and preference during storage by packing Geochang, Yeongju, and Yesan apples in functional MA film. Therefore, this study aims to propose a method to secure the quality safety of apples by investigating the effect of sugar change on the preference of apples during storage by packing apples in three regions in functional MA films.

COMPARISON OF MAINTENANCE OF CANAL CURVATURE IN CURVED CANALS SHAPED WITH FOUR DIFFERENT FILE TYPES (만곡근관에서 수종의 File을 이용한 근관형성시 만곡도 변화에 대한 비교 연구)

  • Shin, Joo-Hee;Kwon, O-Yang;Yoon, Soo-Han
    • Restorative Dentistry and Endodontics
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    • v.22 no.1
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    • pp.388-395
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    • 1997
  • Maintaining the original canal path during instrumentation is a challenge in narrow curved canals. This study compared the maintenance of the original canal path of curved root canals during instrumentation with two kinds of stainless steel K-files(Brassler USA & Mani Japan), K-flexofiles(Maillefer Swiss) and Ni-Ti files(Brassler USA, Savannah, GA) using circumferential filing technique to # 40 MAF on 60 extracted human molars. Buccal and mesial canals with minimal initial curvature of 20 degrees were used. The maximal initial curvature was 41.5 degrees. Sixty curved canals divided into four groups according to file type(Group 1 : Ni-Ti file, Group 2 : K-flexofile, Group 3: K-file(Brassler), Group 4 : K-file(Mani)). Radiographs of canals were obtained before and after canal shaping. And postoperative radiographs were compared with preoperative radiographs using superimposition method. Data analysis was performed using Covariance analysis and paired-comparison test. The results observed were as follow ; 1. The angle of curvature was better maintained with Ni-Ti file than with stainless steel files. (p<0.01) 2. There was no significant difference in maintaining canal curvature between K-flexofile, Brassler$^{(R)}$ K-file and Mani$^{(R)}$ K-file, although there was some differences in mean values of postoperative canal curvature. 3. Paired-comparison t test revealed significant differences within each of the three stainless steel file types when comparing the mean differences before and after instrumentation, but no significant differences were observed within Ni-Ti file group.

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