• Animal Bioscience
• /
• 제37권1호
• /
• pp.131-141
• /
• 2024

Objective: This experiment aimed to explore the protective action of dietary supplementation with isoquinoline alkaloids (IA) from Macleaya cordata on lipopolysaccharide (LPS)-induced liver injury in broilers. Methods: Total 216 healthy broilers were selected in a 21-d trial and assigned randomly to the following 3 treatments: control (CON) group, LPS group, and LPS+IA group. The CON and LPS groups were provided with a basal diet, whereas the LPS+IA group received the basal diet supplemented with 0.6 mg/kg Macleaya cordata IA. Broilers in LPS and LPS+IA groups were intraperitoneally injected with LPS (1 mg/kg body weight) at 17, 19, and 21 days of age, while those in CON group were injected with equivalent amount of saline solution. Results: Results showed LPS injection caused systemic and liver inflammation in broilers, inhibited immune function, and ultimately lead to liver injury. By contrast, supplementation of IA ameliorated LPS-induced adverse change in serum parameters, boosted immunity in LPS+IA group. Furthermore, IA suppressed the elevation of hepatic inflammatory cytokines and caspases levels induced by LPS, as well as the expressions of genes related to the toll-like receptor 4 (TLR4)/myeloid differentiation primary response 88 (MyD88)/nuclear factor-kappa B (NF-κB) pathway. Conclusion: Dietary inclusion of 0.6 mg/kg Macleaya cordata IA could enhance immune function of body and inhibit liver damage via inactivating TLR4/MyD88/NF-κB signaling pathway in broilers.

• Asian-Australasian Journal of Animal Sciences
• /
• 제33권6호
• /
• pp.913-920
• /
• 2020

Objective: The objective of this experiment was to compare conventional antioxidants and plant extracts for oxidative stress control in lambs fed a high-concentrate diet. Methods: Forty-eight male Dorper×Santa Ines lambs with an initial weight of 20±1.49 kg and 60 days of age, were used to evaluate the effects of feeding a combination of Macleaya cordata and Magnolia officinalis plant extracts (0 vs 320 mg/kg dry matter [DM]) in combination with selenium+vitamin E (0 vs 100 IU/kg DM of vitamin E and 0.1 mg/kg DM of selenium) in a completely randomized block design in a 2×2 factorial arrangement. The animals were housed in individual pens and received a high-concentrate diet consisting of 80% whole corn and 20% protein pellet for 60 days. The animals were weighed at the beginning of the experiment and every 14 days for performance monitoring. Three blood samplings were performed during the experimental period for the evaluation of oxidative and protein parameters. Results: The treatments with vitamin E and selenium as additives had a positive influence on final weight, daily weight gain, carcass weight, and selenium content in longissimus muscle (p = 0.01). Plant extracts tended to improve final weight (p = 0.064) and daily weight gain (p = 0.059), showing similar effect as selenium and vitamin E. There was no effect of treatment on blood proteins, indicating that the animals were healthy throughout the experiment. Conclusion: The use of plant extracts had a similar effect as the addition of selenium and vitamin E, with dietary inclusion of additives resulting in better performance of lambs but both supplements did not have strong influence on oxidative stress.

• Asian-Australasian Journal of Animal Sciences
• /
• 제31권9호
• /
• pp.1474-1480
• /
• 2018

Objective: Isoquinoline Alkaloids, derived from one plant (Macleaya cordata) can be an alternative when it is desired to increase performance in feedlot cattle. However, results on these nutritional additives in high energy diets in ruminants are still incipient in literature. In this context, the objective of this study was to evaluate performance and carcass traits of feedlot bulls supplemented with sanguinarine, the main alkaloid presents in Macleaya cordata in high energy density diets. Methods: Thirty-two crossbred Angus-Nelore bulls with mean initial body weight of $365{\pm}10kg$ and mean initial age of $11{\pm}3$ months were used. The experiment lasted 119 days, with 14 days of adaptation and 105 experimental days. Experimental diet consisted of 85% whole corn grains and 15% protein-vitamin-mineral nucleus and supplied ad libitum. Treatments consisted of a control diet (CON) and a diet with sanguinarine supplementation (SAN) at a dosage of 4 g of product sufficient to provide 6 mg of sanguinarine/d. Experimental design was completely randomized. Results: Dry matter intake, average daily gain and feed conversion were similar (p>0.05) between treatments. However, SAN group animals had higher carcass yield (p = 0.045) and were more efficient in the transformation of dry matter consumed in carcass gain (p = 0.046) than CON. In addition, haptoglobin, increased throughout feedlot duration meaning high challenge for the animals due to the diet, but this behavior was similar (p>0.05) between treatments. Conclusion: Sanguinarine produced positive results in relation to carcass yield and could be used as an additive for bulls fed diets receiving high energy density diet.

• Natural Product Sciences
• /
• 제9권2호
• /
• pp.73-79
• /
• 2003

To discover the sources with antioxidative activity in traditional medicines, 100 extracts of Korean medicinal plants were screened for their scavenging effect on peroxynitrite $(ONOO^{-})$ and total reactive oxygen species (ROS). The potency of total ROS scavenging activity was shown in the extracts of 25 plants, and 4 of their species, Macleaya cordata R. Br., Salvia plebeia R. Br., Cassia tora L. and Angelica gigas Nakai, had a greater effect with $IC_{50}$ values of $1.7{\pm}0.36$, $4.3{\pm}1.08$, $4.9{\pm}0.17$ and $5.8{\pm}1.01\;{\mu}g/ml$, respectively, than that of trolox, positive control $(7.61{\pm}0.12\;{\mu}g/ml)$. Another 35 extracts exhibited inhibitory effect of below 50 percent at $100\;{\mu}g/ml$ of sample concentrations on total ROS, while the rest observed total ROS generators rather than scavengers. The peroxynitrite scavenging activities were observed in the greater part of the plants tested. Five of them, Schisandra chinensis Baill, Campsis grandiflora (Thunb.) K. Schum., Cedrela sinensis A. Juss., Pleuropterus multiflorus Turcz. and Veronica linariaefolia Pall represented scavenging activities on peroxynitrite twice as strong with $IC_{50}$ Values of $0.48{\pm}0.10$, $0.59{\pm}0.15$, $0.60{\pm}0.10$, $0.64{\pm}0.10$ and $0.91{\pm}0.23\;{\mu}g/ml$, respectively, as that of penicillamine $(1.72{\pm}0.05\;{\mu}g/ml)$, positive control. Consequently, 25 species of the entire plants tested, exhibited scavenging activities on total ROS and $ONOO^{-}$, Salvia plebeia R. Br., Macleaya cordata R. Br., Cassia tora L. and Angelica gigas Nakai exerted potent scavenging activities on both radicals.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2019년도 춘계학술대회
• /
• pp.25-25
• /
• 2019

1953년 7월 27일 정전협정 이후 한반도를 가로지르는 DMZ (Demilitarized zone: 비무장지대)가 설정되었다. DMZ에 출입은 군사정전위원회의 허가가 있어야 가능하기에 군사시설 및 군작전지역을 포함하여 DMZ 및 민간인통제선 이북은 민간인 접근의 제한으로, 단절된 공간으로 변모하였다. DMZ는 우리나라의 대표적인 동고서저의 지형 및 다양한 생태환경을 두루 갖추고 있어 중요한 생태적 요충지로 평가받고 있다. 산림지역은 민간인통제지역까지 합치면 40여개의 산지가 분포하고 있으며, 하천은 크게 15개의 크고 작은 하천이 위치하고 있다. 남방한계선 수계를 따라 일부지역들은 하천 배후습지, 묵논습지 등 습원형태로도 많이 발견되고 있다. 이런 습원지역들은 대부분 미확인 지뢰지대로 접근이 제한적인 만큼 지난 세월의 흔적들을 고스란히 담고 있다. 1965년 한국자연보존연구회는 30여명의 국내학자를 동원하여 서울에서 가까운 DMZ 인접지역에 대한 예비적인 학술조사를 실시하였으며, 이를 계기로 1966년 10월부터 1968년 9월까지 2년간에 걸쳐 한국자연보존위원회 34명의 국내학자와 미국 스미소니언연구소(Smithsonian Institution) H. K. Buechner 박사를 대표로 5명의 국외학자가 공동으로 종합적인 학술조사를 수행하였다. 이는 DMZ 연구의 시작을 알렸으며, 이후 남북관계나 시대여건에 맞추어 간헐적으로 다양한 조사 및 연구들이 이어져오고 있다. 2015년부터 2018년까지 DMZ 철책주변 현장조사를 통해 확인된 식물현황은 964분류군이다. 군시설로 인해 식물서식환경이 제한적인 상황이지만 척박한 환경에 적응하고 있는 식물은 다양하다. DMZ와 맞닿아 있는 7개 지역에 대한 식물현황은 철원 638분류군, 연천 631분류군, 양구 525분류군 순으로 나타났다. 대부분 중동부 지역에 높은 식물종수가 나타났으며, 서부와 동부의 식물들이 고르게 나타나는 결과로 보인다. 큰잎쓴풀, 봉래꼬리풀 등 희귀식물도 있는 반면, 잠재적으로 생태계에 영향을 줄 수 있는 침입외래식물도 다수 포함되어 있어서, 지속적인 모니터링 및 관리가 필요하다. 그 중에서도 양구지역에 새롭게 발견된 Macleaya cordata (Willd.) R.Br.(양귀비과)는 독성이 포함된 식물이며, 인제에서 2014년에 발견된 미역취아재비는 DMZ일원에 제한적으로 분포하는 종이였으나 현재 연천에서 발견되는 등 확산에 대한 관리방안이 필요하다.