• The Journal of Korean Society of Virology
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• v.27 no.1
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• pp.9-17
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• 1997

Hepatitis C virus (HCV) E2 protein is known to be one of putative envelope proteins. To develop a sensitive detection method for HCV infected tissues and cells, monoclonal antibodys (MAbs) to the E2 protein of HCV were prepared from mice immunized with recombinant baculovirus-expressing E2 protein (Bac-E2). Several hybridoma clones secreting various levels of MAb were isolated and isotypes of these MAb were determined. One clone (L.2.3.3) was used for ascites production and the E2-MAb was purified and characterized. The L.2.3.3 reacted well with both Bac-E2 and E. coli expressed glutathione-S-transferase-E2 (GST-E2) fusion proteins. Using HCV patient sera, E2 envelope protein was found to be localized in the cell membrane boundary both in CHO cells and insect cells which express HCV E2 protein. Similar result was obtained when same cells were treated with the MAb L.2.3.3. These results demonstrated that Bac-E2 protein is capable of eliciting high titer antibody production in mice.

• Journal of Microbiology and Biotechnology
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• v.22 no.2
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• pp.226-229
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• 2012

Bacterial hemoglobin from Vitreoscilla (VHb) is recognized as a good fusion protein for the soluble expression of foreign protein. In this study, we generated a monoclonal antibody (MAb) against VHb for its detection. For the rapid screening of MAb, a protein chip technology based on the Alexa-488 (A488) dye labeling method was introduced. In order to fabricate the chip, the VHb protein was chemically coupled to the chip surface and then the culture supernatants of 84 hybridoma cell lines were spotted onto the VHb chip. The bound MAbs were measured by A488-modified anti-mouse IgG. A single spot (MAb A10) exhibited significantly high signal intensity. The immunoblot analysis evidenced that the MAb A10 can detect VHb-fused proteins with high specificity.

• Journal of Yeungnam Medical Science
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• v.3 no.1
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• pp.103-110
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• 1986

Cytochrome P-450(CP-450) is one of the three components of the liver microsomal enzyme system which hydroxylates fatty acids, hydrocarbons and a variety of drugs and other foreign compounds. Female Balb/c mice were immunized with purified polychlorinated bipheny(PCB)-induced CP-450 LMII. The spleen cells derived from immunized mice were fused with $SP^2$ myeloma cells using polyethylene glycol(PEG 3500). The hybrid cells were selected by hypoxanthine-aminopterine and thymidine(HAT) medium and the culture fluid were screened by enzyme-linked immunosorbent assay to CP450 LMII. The hybrid cess(${\times}10^7$) were innoculated into intraperitoneal cavity of Balb/c mice for the purpose of production of ascitic fluids. Monoclonal antibody(Mab) was purified from ascitic fluid by DEAE cellulose ion exchange chromatography and $I^{125}$-labeled Mab was also confirmed by autoradiography and SDS-polyacrylamide gel electrophoresis (MW : 55,000 and 110,000).

• Journal of Korean Society of Forest Science
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• v.111 no.1
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• pp.136-149
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• 2022

In this study, the performance of a nonlinear mixed-effects (NLME) model used to estimate the stem taper of Pinus densiflora in Gangwon Province was compared with that of a nonlinear fixed-effects (NLFE) model using several performance measures. For the diameters of whole tree stems, the NLME model improved on the performance of the NLFE model by 26.4%, 42.9%, 43.1%, and 0.9% in terms of BIAS, MAB, RMSE, and FI, respectively. For the cross-section areas of whole tree stems, the NLME model improved on the performance of the NLFE model by 67.7%, 44.7%, 45.8%, and 1.0% in terms of BIAS, MAB, RMSE, and FI, respectively. Based on the analysis of 12 relative height classes of tree stems, stem taper estimation performance was also reasonably improved by the NLME model, which showed better MAB, RMSE, and FI at every relative height class compared with those of the NLFE model. In some classes, the NLFE model had better BIAS than the NLME model (stem diameter: 0.05, 0.2, 0.3, and 0.8; stem cross-section area: 0.05, 0.3, 0.5, 0.6, and 1.0). However, the NLME model enhanced the performance of stem diameter and cross-section area estimations at the lowest stem part (0.2 m from the ground). Improvements for stem diameter in terms of BIAS, MAB, RMSE, and FI were 84.2%, 69.8%, 68.7%, and 3.1%, respectively. For stem cross-section areas, the improvements in BIAS, MAB, RMSE, and FI were 98.5%, 70.1%, 68.7%, and 3.1%, respectively. The cross-section area at 0.2 m from the ground occupied 22.7% of total cross-section area. Improvements in estimation of cross-section area at the lowest stem part indicate that stem volume estimation performance could also be enhanced. Although NLME models are more difficult to fit than NLFE models, the use of NLME models as a standard method for the estimating the parameters of stem taper equations should be considered.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.561-562
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• 2003

Hybridoma cell is very important in point of producing monoclonal antibody(Mab). Producing large quantity of Mab is economically valuable. On this experiment, we used one of hybridoma cell line, 5F12 AD3, and treated various antibiotics such as genetitin(G418), ciprofloxacin and minocycline to improve cell viability and we expect that improving cell viability brings higher concentrations of Mab. The optimum concentration of each antibiotics for improving cell viability were 10ug/ml for G418, 1ug/ml or 10ug/ml for ciprofloxacin and 1ug/ml for minocycline.

• Proceedings of the KSME Conference
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• 2003.11a
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• pp.1016-1020
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• 2003

In this paper, we present a technology of producing anew chaotic micromixer, named Micromixer with Arranged Blocks(MAB), and the experimental result of the mixing performance. Chaotic mixing was successfully achieved by introducing periodic perturbation in the field of the channel flow by means of slanted blocks. The MAB was made by an RP(Rapid Prototyping) technology. We performed flow visualization experiments for the quantification of the mixing performance with the MAB. Lyapunov exponent was measured to be 0.3557 and 0.1305 for the block height 0.8 and 0.2 times the channel width.

• Proceedings of the KIEE Conference
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• 2006.07b
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• pp.849-850
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• 2006

최근 자동차 브레이크의 전기적 시스템에 대한 연구가 활발히 진행되고 있다. 이와 관련하여 본 논문에서는 자기 엑추에이터 브레이크 시스템(MABS)을 분석하였다. 주행 도중 차량의 이상 및 긴급 상황 발생시 MABS는 전기적 메커니즘을 통해 차량을 제동할 수 있다. MABS는 회전하고 있는 휠에 초당 수십회 작용하여 점차적이고 효율적으로 휠을 제동한다. 이는 제동시간의 단축과 안정성에 있어 향상된 성능을 보인다. 본 연구에서는 유한요소법을 적용한 시뮬레이션을 통해 MABS의 동작 특성을 분석하고, 실제 자동차의 상황을 가정하여 제동하는데 소요되는 시간 및 작동 회수 등에 대해 분석하였다.

• Horticultural Science & Technology
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• v.35 no.2
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• pp.232-242
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• 2017

The goal of marker-assisted backcrossing (MAB) is to significantly reduce the number of breeding generations required by using genome-based molecular markers to select for a particular trait; however, MAB systems have only been developed for a few vegetable crops to date. Among the types of molecular markers, SNPs (single-nucleotide polymorphisms) are primarily used in the analysis of genetic diversity due to their abundance throughout most genomes. To develop a MAB system in Chinese cabbage, a high-throughput (HT) marker system was used, based on a previously developed set of 468 SNP probes (BraMAB1, Brassica Marker Assisted Backcrossing SNP 1). We selected a broad-spectrum TuMV (Turnip mosaic virus) resistance (trs) Chinese cabbage line (SB22) as a donor plant, constructing a $BC_1F_1$ population by crossing it with the TuMV-susceptible 12mo-682-1 elite line. Foreground selection was performed using the previously developed trsSCAR marker. Background selection was performed using 119 SNP markers that showed clear polymorphism between donor and recipient plants. The background genome recovery rate (% recurrent parent genome recovery; RPG) was good, with three of 75 $BC_1F_1$ plants showing a high RPG rate of over 80%. The background genotyping result and the phenotypic similarity between the recurrent parent and $BC_1F_1$ showed a correlation. The plant with the highest RPG recovery rate was backcrossed to construct the $BC_2F_1$ population. Foreground selection and background selection were performed using 169 $BC_2F_1$ plants. This study shows that, using MAB, we can recover over 90% of the background genome in only two generations, highlighting the MAB system using HT markers as a highly efficient Brassica rapa backcross breeding system. This is the first report of the application of a SNP marker set to the background selection of Chinese cabbage using HT SNP genotyping technology.

• BMB Reports
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• v.49 no.10
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• pp.554-559
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• 2016

Mycobacterium abscessus, a member of the group of non-tuberculous mycobacteria, has been identified as an emerging pulmonary pathogen in humans. However, little is known about the protective immune response of antigen-presenting cells, such as dendritic cells (DCs), which guard against M. abscessus infection. The M. abscessus gene MAB1843 encodes ᴅ-alanyl-ᴅ-alanine dipeptidase, which catalyzes the hydrolysis of ᴅ-alanyl-ᴅ-alanine dipeptide. We investigated whether MAB1843 is able to interact with DCs to enhance the effectiveness of the host's immune response. MAB1843 was found to induce DC maturation via toll-like receptor 4 and its downstream signaling pathways, such as the mitogen-activated protein kinase and nuclear factor kappa B pathways. In addition, MAB1843-treated DCs stimulated the proliferation of T cells and promoted Th1 polarization. Our results indicate that MAB1843 could potentially regulate the immune response to M. abscessus, making it important in the development of an effective vaccine against this mycobacterium.

• The Korean Journal of Cytopathology
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• v.8 no.1
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• pp.27-34
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• 1997

Pneumocystis carinli is an established cause of pulmonary infections in immuno-compromised hosts. Several cytoiogical stains, such as Papanicolaou, Gomori methenamine sliver(GMS) and Diff-Quik have been used for detection of the organism, but occasionally can be laborious and, due to a degree of nonspecificity, may be misleading. We evaluated the diagnostic utility of immunocytochenmical stains that recognize P. carinii in bornchoalveolar lavage from experimentally Induced P. carinii pneumonia rats(n=15). In audition to routine stains for diagnosis by morphologic recognition of P. carinii on Papanicolaou, GMS and Diff-Quik stains, bronchoalveolar lavage samples were reacted with immunocytochemical stains using monoclonal antibodies(MAB) 092 and 902. In bronchoalveolar lavage P. carinii organisms were detected In 9 of 10 cases(90%) using each MAB 092 and 902, whereas GMS and Diff-Quik stains demonstrated P. carinii in 13(86%) and 11(73%) of 15 cases respectively. In lung tissue specimens(n=15) P. carinii organisms were well identified on GMS stain and immunohistochemical stains using MAB 092 and 902 in ail cases. We believe that the immunocytochemical staining using MAB 092 and/or 902 is a very useful and diagnostic tool In addition to GMS and Diff-Qulk stain to detect P. carinii organisms in bronchoalveolar lavage.