• Title/Summary/Keyword: MYO

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Enzyme Kinetic Study for Degradation of Phytic acid by Phytase (Phytase에 의한 phytic acid의 분해 반응속도 연구)

  • Kim, Jae-Seok;Lee, Jae-Heung
    • KSBB Journal
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    • v.26 no.4
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    • pp.300-304
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    • 2011
  • Phytases are hydrolytic enzymes that catalyze the sequential hydrolysis of phytic acid (myo-inositol-1,2,3,4,5,6-hexakisphosphate) to myo-inositols with lower numbers of phosphate groups. Two types of phytases have been identified which initiate hydrolysis of the phytic acid at either the 3- or 6- position of the inositol ring. In the present investigation, a mathematical model was proposed and computed to estimate maximum enzyme reaction rate constants which fit the experimental data obtained by other authors. Although the data points were scattered to some extent, good agreement was found between the model and the experiment data. It appears that the maximum rate constants of removal of the first, second, and third phosphate groups were not equal. Also there was neither a steady trend upward or downward in the rate constants with the stepwise hydrolysis reactions.

Screening of Phytase Overproducing Strains in Aspergillus spp. by UV Mutagenesis

  • Lee, Eung-Suek;Paik, In-Kee;Hahm, Young-Tae
    • Mycobiology
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    • v.28 no.3
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    • pp.119-122
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    • 2000
  • Phytases (myo-inositol hexakisphosphate phosphohydrolase; EC 3.1.3.8) are enzymes which catalyze the hydrolisys of phytate into myo-inositol and inorganic phosphates. Phytases are found in plants and a variety of microorganisms. Aspergillus species were treated with 254 nm of UV irradiation for the screening of phytase overproducing mutant strains. At 15 minute irradiation, the survivals of population were less than 5%, and UV irradiation time was decided at 20 minute for the isolation of mutant strains. Four UV mutant strains in A. oryzae (YUV-47, -169, -341, -511) and six in A. ficuum (FUV-17, -36, -69, -193, -317, -419) were isolated on PSM media containing ammonium phosphate. The specific enzyme activities of A. ficuum mutants are 110 to 140% higher than that of wild type.

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Synthesis of Cyclitol Derivatives (IV) Electrolytic Reduction of DL-epi-inosose-2 (Cyclitol 유도체 합성에 관한 연구 (제4보) DL-epi-inosose-2의 전해환원)

  • Sohn, Joo-Hwan;Nam, Chong-Woo;Park, Heung-Cho
    • Journal of the Korean Chemical Society
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    • v.16 no.2
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    • pp.93-99
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    • 1972
  • To obtain the various kinds of inositol stereomers, we have selected the process of electrolytic reduction of DL-epi-inosose-2 using Ni, Ta, Cu, Mo, Pb, Sn, W, Cd and Hg etc., as cathode. We think that this process gives greater variety than chemical processes. DL-epi-inosose-2 was synthesized by chemical oxidation of myo-inositol, dissolved in aqueous solution of potassium hydroxide, and used as electrolytic solution. To prevent anodic oxidation of cathode products the H-type diaphragm cell was used. As the results of paper chromatography of cathodic products, we obtained the $R_f$ values of myo-inositol and epi-inositol were in good agreement with literature values.

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Purification of Phytase from Aspergillus ficuum and Production of Anti-phytase Antibody (Aspergillus ficuum의 Phytase의 정제와 Anti-phytase 항체생산)

  • Kim, Keun
    • The Korean Journal of Mycology
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    • v.27 no.4 s.91
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    • pp.299-303
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    • 1999
  • Phytase(myo-inositol-hexakis phosphate 3-phosphohydrolase, E C 3.1.3.8) sequentially hydrolyzes phytate to myo-inositol and inorganic phosphate. Phytase of Aspergillus ficuum was purified to homogeneity using ultrafiltration, cation exchange column and anion exchange column. It's molecular weight is estimated as around 90,000 by SDS-PAGE. Antibody against the phytase was produced by immunizing mice with the purified phytase. The titer of the antibody was determined to be 1/25,000.

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A Study on UAV Control System using Myos (Myo를 이용한 UAV 제어 시스템 설계 연구)

  • Kim, Hyeok;Kim, Donguk;Sung, Yunsick
    • Annual Conference of KIPS
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    • 2016.04a
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    • pp.663-666
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    • 2016
  • 최근에 증가한 무인항공기(Unmanned Aerial Vehicle, UAV) 활용에 따라 다양한 제어 방법이 도입되고 있다. 일반적으로는 UAV 조종기를 통해서 직접 제어하는 방법이 많이 활용되고 있다. 하지만, 세밀한 UAV 제어를 위해서는 직관적인 사용자 인터페이스가 필요하다. 이 논문에서는 Myo로 다종 UAV를 제어하는 방법을 제안한다. 이를 위해서 기 개발된 그라운드스테이션과 UAV를 연결하고 제어하는 방법을 소개한다. UAV를 체감형 인터페이스로 제어함으로써 직관적인 제어가 가능하다.

Motion Recognition Sensor-based UAV Control Signal Generation Method (모션 인식 센서 기반의 UAV 제어 신호 생성 방법)

  • Kim, Donguk;Kim, Hyeok;Sung, Yunsick
    • Annual Conference of KIPS
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    • 2016.04a
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    • pp.682-685
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    • 2016
  • 최근 무인항공기(Unmanned Aerial Vehicle, UAV)가 많은 보급됨에 따라서 다양한 방법으로 이를 제어하기 위한 노력들이 이루어지고 있다. 그 중 NUI/NUX를 이용하여 UAV를 접목하려는 시도가 있다. 전통적인 NUI/NUX는 가상현실에서 시각적인 체감에 많이 의존하고 있지만 동작 인식 센서를 기반으로 가상 에이전트를 제어하는 기법들도 소개되고 있다. 이 논문에서는 모션 인식 센서인 Myo를 사용하여 UAV를 제어하는 시스템을 제안한다. 제안한 시스템은 지상 통제소(Ground Control Station, GCS)의 서브시스템이며 전통적인 드론 조종 방법과 같이 양팔로 제어하기 때문에 두 개의 Myo를 사용한다.

Surface Chemical Properties of Surface Active Aminocyclitol Derivatives (계면활성(界面活性) 아미노싸이클리톨 유도체(誘導體)의 계면화학적(界面化學的) 성질(性質))

  • Sohn, Joo-Hwan;Lee, Chang-Sup
    • Journal of the Korean Applied Science and Technology
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    • v.4 no.1
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    • pp.45-52
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    • 1987
  • Surface chemical properties including surface tension, Ross-Miles foaming power, foam stabilities, emulsifying properties, emulsion stabilities, effectiveness of dispersion, dispersion stabilites of the quaternary ammonium salt type deoxyaminoimositol derivatives such as sixteen kinds of dimethylafkyl-deoxyscyllo-inosityl- dimethylalkyl-2-deoxy-2-myo-inosityl-, dimethylakyl-2-deoxy-2-epi-inosityl- and dimethylalkyl-4-deoxy-4-myo-inosityl ammonium chlorides are tested. And critical micelle concentration of these compounds are estimated from the curves of surface tension and concentration. The experimental results show that the members of this class of compounds have necessary surface active properties to make them effective surfactants.