• Title/Summary/Keyword: MUD

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Osmotic-inducible Gene Expression using MudJ(Km.lac) Operon Fusion in Salmonella typhimurium (Salmonella typhimurium에서 MudJ(Km.lac) 오페론 융합을 이용한 삼투유도유전자의 발현)

  • 주성관;우영대;허연주;안정선;박용근
    • Korean Journal of Microbiology
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    • v.29 no.4
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    • pp.215-220
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    • 1991
  • MudJ(Km.lac) operon fusions were used in the identification of osmotic-inducible genetic(osi) loci in Salmonella typhimurium. Expression of osi::lacZ(osi5001, 5027) genes were dramatically induced 39-189 fold when the osmolarity was increased. Seven osm::lacZ genes were constituvely expressed under both low and high osmotic strength. The osi5001::lacZ fusion strains showed the enhanced osmotolerance and the reduced expression of the osi5001::lacZ in the presence of 1mM proline or betaine as osmoprotectants. Four osmotic inducible genetic loci were mapped into 36 (YK531), 44 (YK504), 57 (YK501) and 84 (YK528) map unit by testing the cotransduction frequency.

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Applicability Assessment of Acid Treated Red Mud as Adsorbent Material for Removal of Six-valent Chromium from Seawater (해수에서 6가 크롬 제거를 위한 흡착제로서의 산처리 적니 적용성 검토)

  • Kang, Ku;Um, Byung-Hwan;Kim, Young-Kee;Park, Seong-Jik
    • Journal of The Korean Society of Agricultural Engineers
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    • v.55 no.5
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    • pp.17-23
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    • 2013
  • Six-valent chromium ($Cr^{6+}$) is a highly toxic pollutant, supplied in a variety of industrial activities such as leather tanning, cooling tower blowdown, and plating. Herein, we investigated the removal of $Cr^{6+}$ from aqueous phase using low-cost adsorbents. Steel slag, montmorillonite, illite, kaolinite, red mud, and acid treated red mud with 0.5, 1.0, and 2.0 M HCl were used as adsorbent for the removal of $Cr^{6+}$ and the results showed that acid treated red mud with 2.0 M HCl (ATRM-2.0 M) had higher adsorption capacity of $Cr^{6+}$ than other adsorbents used. Accordingly, $Cr^{6+}$ removal by ATRM-2.0 M were studied in a batch system with respect to changes in initial concentration of $Cr^{6+}$, initial solution pH, adsorbent dose, adsorbent mixture, and seawater. Equilibrium sorption data were described well by Freundlich isotherm model. The influence of initial solution pH on $Cr^{6+}$ adsorption was insignificant. The use of the ATRM-2.0 M alone was more effective than mixing it with other adsorbents including red mud, zeolite, oyster shell, lime stone, and montmorillonite for the removal of $Cr^{6+}$. The $Cr^{6+}$ removal of the ATRM-2.0 M was slightly less in seawater than deionized water, resulting from the presence of anions in seawater competing for the favorable adsorption site on the surface of ATRM-2.0 M. It was concluded that the ATRM-2.0 M can be used as a potential adsorbent for the removal of $Cr^{6+}$ from the aqueous solutions.

Adsorption Characteristics of Multi-Metal Ions by Red Mud, Zeolite, Limestone, and Oyster Shell

  • Shin, Woo-Seok;Kang, Ku;Kim, Young-Kee
    • Environmental Engineering Research
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    • v.19 no.1
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    • pp.15-22
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    • 2014
  • In this study, the performances of various adsorbents-red mud, zeolite, limestone, and oyster shell-were investigated for the adsorption of multi-metal ions ($Cr^{3+}$, $Ni^{2+}$, $Cu^{2+}$, $Zn^{2+}$, $As^{3+}$, $Cd^{2+}$, and $Pb^{2+}$) from aqueous solutions. The result of scanning electron microscopy analyses indicated that the some metal ions were adsorbed onto the surface of the media. Moreover, Fourier transform infrared spectroscopy analysis showed that the Si(Al)-O bond (red mud and zeolite) and C-O bond (limestone and oyster shell) might be involved in heavy metal adsorption. The changes in the pH of the aqueous solutions upon applying adsorbents were investigated and the adsorption kinetics of the metal ions on different adsorbents were simulated by pseudo-first-order and pseudo-second-order models. The sorption process was relatively fast and equilibrium was reached after about 60 min of contact (except for $As^{3+}$). From the maximum capacity of the adsorption kinetic model, the removal of $Pb^{2+}$ and $Cu^{2+}$ were higher than for the other metal ions. Meanwhile, the reaction rate constants ($k_{1,2}$) indicated the slowest sorption in $As^{3+}$. The adsorption mechanisms of heavy metal ions were not only surface adsorption and ion exchange, but also surface precipitation. Based on the metal ions' adsorption efficiencies, red mud was found to be the most efficient of all the tested adsorbents. In addition, impurities in seawater did not lead to a significant decrease in the adsorption performance. It is concluded that red mud is a more economic high-performance alternative than the other tested adsorption materials for applying a removal of multi-metal in seawater.

Synthesis of Pellet-Type Red Mud Adsorbents for Removal of Heavy Metal Ions (중금속이온제거를 위한 입자형 적니흡착제의 제조)

  • 김정식;한상원;황인국;배재흠;최우진
    • Resources Recycling
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    • v.9 no.1
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    • pp.44-51
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    • 2000
  • Red mud is generated as a by-product in the production of $Al(OH)_{3}/Al_2O_3$ from bauxite ore. In this study the pellet-type adsorbents have been made from the red mud, and their adsorption capacities of heavy metal ions have been tested. The pellet-type adsorbents were synthesized to utilize the excellent adsorption capacity of the powder-type adsorbent for industrial application. The pellet-type adsorbents were prepared by mixing several kinds of additives with the red mud. It is found that the pellet-type adsorbent, made by sintering a mixture of red mud (96.0 wt%), polypropylene (2.5%), fly ash (0.5 w%), and sodium metasilicate(1.0 wt%) at $1200^{\circ}C$ for 30 minutes, has the highest adsorption capacity. in this work, the two kinds of pellet-type adsorbents (bead-type, crushed-type) were prepared. The crushed-type adsorbent was found to show a better adsorption/desorption performance than the bead-type adsorbent. The crushed-type adsorbent showed a good adsorption capacity of $Pb^{2+}$ like the powder-type adsorbent.

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SE-CAC: A Novel Call Admission Control Scheme for Multi-service IDMA Systems

  • Ge, Xin;Liu, Gongliang;Mao, Xingpeng;Zhang, Naitong
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.5 no.5
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    • pp.1049-1068
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    • 2011
  • In this paper a simple and effective call admission control (CAC) scheme is proposed for the emerging interleave-division multiple-access (IDMA) systems, supporting a variety of traffic types and offering different quality of service (QoS) requirements and priority levels. The proposed scheme is signal-to-interference-plus-noise ratio (SINR) evolution based CAC (SE-CAC). The key idea behind the scheme is to take advantage of the SINR evolution technique in the process of making admission decisions, which is developed from the effective chip-by-chip (CBC) multi-user detection (MUD) process in IDMA systems. By virtue of this semi-analytical technique, the MUD efficiency can be estimated accurately. Additionally, the computational complexity can be considerably reduced. These features make the scheme highly suitable for IDMA systems, which can combat intra-cell interference efficiently with simple CBC MUD. Analysis and simulation results show that compared to the traditional CAC scheme considering MUD efficiency as a constant, the proposed SE-CAC scheme can guarantee high power efficiency and throughput for multimedia traffic even in heavy load conditions, illustrating the high efficiency of CBC MUD. Furthermore, based on the SINR evolution, the SE-CAC can make accurate estimation of available resource considering the effect of MUD, leading to low outage probability as well as low blocking and dropping probability.

Genomic Organization and Isoform-Dependent Expression Patterns of Wap65 genes in Various Tissues during Immune Challenges in the Mud Loach Misgurnus mizolepis

  • Kim, Yi Kyung;Cho, Young Sun;Lee, Sang Yoon;Nam, Yoon Kwon
    • Fisheries and Aquatic Sciences
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    • v.17 no.4
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    • pp.471-478
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    • 2014
  • Genomic organization, including the structural characteristics of 5'-flanking regions of two 65-kDa protein (WAP65) isoform genes associated with warm temperature acclimation, were characterized and their transcriptional responses to immune challenges were examined in the intestine, kidney and spleen of the mud loach (Misgurnus mizolepis; Cypriniformes). Both mud loach Wap65 isoform genes displayed a 10-exon structure that is common to most teleostean Wap65 genes. The two mud loach Wap65 isoforms were predicted to possess various stress- and immune-related transcription factor binding sites in their regulatory regions; however, the predicted motif profiles differed between the two isoforms, and the inflammation-related transcription factor binding motifs, such as NF-${\kappa}B$ and CREBP sites, were more highlighted in the Wap65-2 isoform than the Wap65-1 isoform. The results of qRT-PCR indicated that experimental immune challenges using Edwardsiella tarda, lipopolysaccharide or polyI:C induced the Wap65-2 isoform more than Wap65-1 isoform, although modulation patterns in response to these challenges were tissue- and stimulant-dependent. This study confirms that functional diversification between the two mud loach Wap65 isoforms (i.e., closer involvement of Wap65-2 in the acute phase of inflammation and innate immunity) occurs at the mRNA level in multiple tissues, and suggests that such differential modulation patterns between the two isoforms are related to the different transcription factor binding profiles in their regulatory regions.

Behavioral Monitoring System for Mud Shrimp Upogebia major and the Photoresponse to Illumination with Different Wavelength LEDs (쏙(Upogebia major)의 광반응 분석시스템 구축과 발광다이오드(Light-Emitting Diode) 파장별 행동분석)

  • Jang, Jun-Chul;Chung, Jong-Kyun;Hur, Youn-Seong;Song, Jae-Hee;Kim, Jong-Myoung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.4
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    • pp.413-420
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    • 2017
  • The increase in the number of mud shrimps Upogebia major is a concern because of their negative effects on shellfish aquaculture, including Manila clam Venerupis philippinatum along the west coast of Korea. This study developed a behavioral analysis system for aquatic animals using a set of monochromatic light-emitting diode (LED) modules covering the visible light range at similar intervals. Movements of mud shrimp were monitored using a tracking system under illumination with infra-red light and an LED of 660 nm wavelength without provoking stimulation. The minimum light intensity needed to induce a photoresponse by the mud shrimp was $10{\mu}mole/m^2/s$ under the conditions tested. Of the six kinds of LED illuminations tested, the most sensitive response was obtained with illumination with the 505 nm LED, followed in order by LEDs with peak wavelengths of $525nm{\fallingdotseq}465nm$ > $405nm{\fallingdotseq}590nm$ > 660 nm. These findings should help to identify LED sources that efficiently induce movement of the mud shrimp and also for monitoring movement without stimulating.

Estimation of Entrainment Rate of Fluid Mud using Annular Flume (환형수조를 이용한 머드유동층의 연행부상률 산정)

  • Kim, Dong-Ho;Kim, Won-Kyu;Hwang, Kyu-Nam
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.28 no.5
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    • pp.257-264
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    • 2016
  • In this study, experiments for measurements of entrainment rate of fluid mud were carried out using annular flume domestically for the first time. Six entrainment tests using kaolinite sediments were conducted with different initial concentrations of fluid mud. It is shown that sediment settling counteracts the otherwise buoyancy dependent entrainment of fluid mud, and that the settling effect leads to a measurably decreased entrainment rate at higher Richardson numbers in comparison with entrainment of salt water, due to additional dissipation of turbulent kinetic energy in the interfacial layer. Through the comparison with previous other studies, the overall performance of the annular flume, the experimental procedure and the test results in simulating the entrainment of fluid mud are shown to be good enough to verify.

Recycling of Red Mud as Plastic Fillers (플라스틱 Filler로서의 적니의 재활용)

  • Kim, Jeong Ho;Soh, Young Soo;Kim, Joon-Hyung
    • Clean Technology
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    • v.5 no.2
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    • pp.45-52
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    • 1999
  • Recycling of red mud from the aluminium manufacturing process was investigated to be utilized as plastic fillers. High density polyethylene(HDPE), low density polyethylene(LDPE) and polypropylene(PP) were found to be the suitable plastic material for which red mud can be used as fillers. With the addition of red mud the plastic showed red brown color. As the ratio of amount of red mud to plastic increased, the tensile strength increased while the Izod impact strength decreased. About five percent of ethylene vinyl alcohol(EVA) was needed as an additive to prevent the lowering of impact strength. Maleic anhydride modified polypropylene was effective for reduction of impact strength lowering of PP. Mixed waste plastics containing LDPE, HDPE, PP, polystyrene and ABS could also accommodate red mud as fillers. In this case, significant loss in mechanical properties were observed due to immiscibility between the components. Ethylene propylene rubber(EPR) and styrene butadiene styrene block copolymer (SBS) could be used to improve the impact properties of the commingled waste plastics.

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Characteristics on Compressive Strength of Cement Paste with Content of LRM Neutralized by Nitric Acid and Sulfuric Acid (질산 및 황산에 의해 중화된 액상화 레드머드의 첨가량에 따른 시멘트 페이스트의 압축강도 특성)

  • Kang, Suk-Pyo;Lee, Hee-Ra;Kang, Hye-Ju;Lee, Byeong-Gi
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.7 no.4
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    • pp.333-340
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    • 2019
  • Red mud is an industrial by-product produced during the manufacturing aluminum hydroxide (Al(OH)3) and aluminum oxide(Al2O3) from Bauxite ores. In Korea, aproximately 2 tons of red mud in a sludge form with 50% moisture content is produced when 1ton of Al2O3 is produced through the Bayer process. Neutralization of red mud will help to reduce the environmental impact caused due to its storage and also lessen significantly the ongoing management of the deposits after closure. It will also open opportunities for re-use of the residue which to date have been prevented because of the high pH. Moreover, attention to liquefied red mud(LRM) that does not require heating and grinding process for recycling is needed. In this paper, characteristics of compressive strength for cement paste with content of LRM neutralized by nitric acid and sulfuric acid. The results showed that compressive strength of cement paste with neutralized LRM is higher than that of cement paste with LRM.