• Title/Summary/Keyword: MTR

Search Result 84, Processing Time 0.019 seconds

Quantitative analysis of oral disease-causing bacteria in saliva among bacterial culture, SYBRgreen qPCR and MRT-PCR method (타액내 구강질환 원인 균의 세균배양법, SYBR green qPCR법, MRT-PCR법 간의 정량분석)

  • Park, Yong-Duk;Oh, Hye-Young;Park, Bok-Ri;Cho, Ara;Kim, Dong-Kie;Jang, Jong-Hwa
    • Journal of Korean society of Dental Hygiene
    • /
    • v.17 no.2
    • /
    • pp.319-330
    • /
    • 2017
  • Objectives: The purpose of this study was to compare SYBR Green qPCR, TaqMan, and bacterial selective medium cultures for accurate quantitative analysis of oral microorganisms. Methods: The SYBR Green method is widely used to analyze the total amount of oral microorganisms in oral saliva. However, in this study, MTR-PCR method based on TaqMan method was performed using newly developed primers and probes. In addition, it was designed to confirm the detection agreement of bacteria among bacteria detection method. Results: As a result of MRT-PCR and SYBR Green qPCR analysis, more than 40 times (0.9-362.9 times) bacterium was detected by MRT-PCR. In addition, more bacteria were detected in saliva in the order of MRT-PCR, SYBR Green qPCR, and bacterium culture, and the results of MRB-PCR and SYBR Green qPCR showed the highest agreement. The agreement between the three methods for detecting P. intermedia was similar between 71.4 and 88.6%, but the agreement between MRT-PCR and SYBR Green qPCR was 80% for S. mutans. Among them, the number of total bacteria, P. intermedia and S. mutans bacteria in saliva was higher than that of SYBR Green qPCR method, and bacterium culture method by MRT-PCR method. P. intermedia and S. mutans in saliva were detected by MRT-PCR and MRT-PCR in 88.6% of cases, followed by the SYBR Green qPCR method (80.0%). Conclusions: The SYBR Green qPCR method is the same molecular biology method, but it can not analyze the germs at the same time. Bacterial culturing takes a lot of time if there is no selective culture medium. Therefore, the MRT-PCR method using newly developed primers and probes is considered to be the best method.

APPLICATION OF WIFI-BASED INDOOR LOCATION MONITORING SYSTEM FOR LABOR TRACKING IN CONSTRUCTION SITE - A CASE STUDY in Guangzhou MTR

  • Sunkyu Woo;Seongsu Jeong;Esmond Mok;Linyuan Xia;Muwook Pyeon;Joon Heo
    • International conference on construction engineering and project management
    • /
    • 2009.05a
    • /
    • pp.869-875
    • /
    • 2009
  • Safety is a big issue in the construction sites. For safe and secure management, tracking locations of construction resources such as labors, materials, machineries, vehicles and so on is important. The materials, machineries and vehicles could be controlled by computer, whereas the movement of labors does not have fixed pattern. So, the location and movement of labors need to be monitored continuously for safety. In general, Global Positioning System(GPS) is an opt solution to obtain the location information in outside environments. But it cannot be used for indoor locations as it requires a clear Line-Of-Sight(LOS) to satellites Therefore, indoor location monitoring system could be a convenient alternative for environments such as tunnel and indoor building construction sites. This paper presents a case study to investigate feasibility of Wi-Fi based indoor location monitoring system in construction site. The system is developed by using fingerprint map of gathering Received Signal Strength Indication(RSSI) from each Access Point(AP). The signal information is gathered by Radio Frequency Identification (RFID) tags, which are attached on a helmet of labors to track their locations, and is sent to server computer. Experiments were conducted in a shield tunnel construction site at Guangzhou, China. This study consists of three phases as follows: First, we have a tracking test in entrance area of tunnel construction site. This experiment was performed to find the effective geometry of APs installation. The geometry of APs installation was changed for finding effective locations, and the experiment was performed using one and more tags. Second, APs were separated into two groups, and they were connected with LAN cable in tunnel construction site. The purpose of this experiment was to check the validity of group separating strategy. One group was installed around the entrance and the other one was installed inside the tunnel. Finally, we installed the system inner area of tunnel, boring machine area, and checked the performance with varying conditions (the presence of obstacles such as train, worker, and so on). Accuracy of this study was calculated from the data, which was collected at some known points. Experimental results showed that WiFi-based indoor location system has a level of accuracy of a few meters in tunnel construction site. From the results, it is inferred that the location tracking system can track the approximate location of labors in the construction site. It is able to alert the labors when they are closer to dangerous zones like poisonous region or cave-in..

  • PDF

Cyanobacteria and Secondary Metabolites (시아노박테리아의 이차대사물질에 대한 연구)

  • Kim, Gi-Eun;Kwon, Jong-Hee
    • KSBB Journal
    • /
    • v.22 no.5
    • /
    • pp.356-361
    • /
    • 2007
  • Cyanobacteria are a very old group of prokaryotic organisms that produce very diverse secondary metabolites, especially non-ribosomal peptide and polyketide structures. Although some cyanobacteria produce lethal toxins such as microcystins and anatoxins, some may be useful either for development into commercial drugs or as biochemical tools. Detection of unknown secondary metabolites was carried in the present study by a screening of 98 cyanobacterial strains from Cyanobiotech GmbH in order to establish a screening process, isolate pure substances and determine their bioactivities. A degenerated polymerase chain reaction technique as molecular approaches has been used for general screening of NRPS gene and PKS gene in cyanobacteria. A putative PKS gene was detected by DKF/DKR primer in 38 strains (38.8%) and PCR amplicons resulted from a presence of NRPS gene were showed by MTF2/MTR2 primer in 30 strains (30.6%), respectively. A screening of interesting strains was performed by comparing PCR screening results with HPLC analyses of extracts. HPLC analysis for a detection of natural products was performed in extracts from biomass. 5 strains were screened for further scale-up processing. 7 pure substances were isolated from the scale-up cultures and tested for bioactivities under consideration to purity, amount and molecular weight of substances. One substance isolated from CBT 635 showed cytotoxic activity. This substance may be regarded as Microcystin LR.

A Study on Knowledge Entity Extraction Method for Individual Stocks Based on Neural Tensor Network (뉴럴 텐서 네트워크 기반 주식 개별종목 지식개체명 추출 방법에 관한 연구)

  • Yang, Yunseok;Lee, Hyun Jun;Oh, Kyong Joo
    • Journal of Intelligence and Information Systems
    • /
    • v.25 no.2
    • /
    • pp.25-38
    • /
    • 2019
  • Selecting high-quality information that meets the interests and needs of users among the overflowing contents is becoming more important as the generation continues. In the flood of information, efforts to reflect the intention of the user in the search result better are being tried, rather than recognizing the information request as a simple string. Also, large IT companies such as Google and Microsoft focus on developing knowledge-based technologies including search engines which provide users with satisfaction and convenience. Especially, the finance is one of the fields expected to have the usefulness and potential of text data analysis because it's constantly generating new information, and the earlier the information is, the more valuable it is. Automatic knowledge extraction can be effective in areas where information flow is vast, such as financial sector, and new information continues to emerge. However, there are several practical difficulties faced by automatic knowledge extraction. First, there are difficulties in making corpus from different fields with same algorithm, and it is difficult to extract good quality triple. Second, it becomes more difficult to produce labeled text data by people if the extent and scope of knowledge increases and patterns are constantly updated. Third, performance evaluation is difficult due to the characteristics of unsupervised learning. Finally, problem definition for automatic knowledge extraction is not easy because of ambiguous conceptual characteristics of knowledge. So, in order to overcome limits described above and improve the semantic performance of stock-related information searching, this study attempts to extract the knowledge entity by using neural tensor network and evaluate the performance of them. Different from other references, the purpose of this study is to extract knowledge entity which is related to individual stock items. Various but relatively simple data processing methods are applied in the presented model to solve the problems of previous researches and to enhance the effectiveness of the model. From these processes, this study has the following three significances. First, A practical and simple automatic knowledge extraction method that can be applied. Second, the possibility of performance evaluation is presented through simple problem definition. Finally, the expressiveness of the knowledge increased by generating input data on a sentence basis without complex morphological analysis. The results of the empirical analysis and objective performance evaluation method are also presented. The empirical study to confirm the usefulness of the presented model, experts' reports about individual 30 stocks which are top 30 items based on frequency of publication from May 30, 2017 to May 21, 2018 are used. the total number of reports are 5,600, and 3,074 reports, which accounts about 55% of the total, is designated as a training set, and other 45% of reports are designated as a testing set. Before constructing the model, all reports of a training set are classified by stocks, and their entities are extracted using named entity recognition tool which is the KKMA. for each stocks, top 100 entities based on appearance frequency are selected, and become vectorized using one-hot encoding. After that, by using neural tensor network, the same number of score functions as stocks are trained. Thus, if a new entity from a testing set appears, we can try to calculate the score by putting it into every single score function, and the stock of the function with the highest score is predicted as the related item with the entity. To evaluate presented models, we confirm prediction power and determining whether the score functions are well constructed by calculating hit ratio for all reports of testing set. As a result of the empirical study, the presented model shows 69.3% hit accuracy for testing set which consists of 2,526 reports. this hit ratio is meaningfully high despite of some constraints for conducting research. Looking at the prediction performance of the model for each stocks, only 3 stocks, which are LG ELECTRONICS, KiaMtr, and Mando, show extremely low performance than average. this result maybe due to the interference effect with other similar items and generation of new knowledge. In this paper, we propose a methodology to find out key entities or their combinations which are necessary to search related information in accordance with the user's investment intention. Graph data is generated by using only the named entity recognition tool and applied to the neural tensor network without learning corpus or word vectors for the field. From the empirical test, we confirm the effectiveness of the presented model as described above. However, there also exist some limits and things to complement. Representatively, the phenomenon that the model performance is especially bad for only some stocks shows the need for further researches. Finally, through the empirical study, we confirmed that the learning method presented in this study can be used for the purpose of matching the new text information semantically with the related stocks.