• Korean Journal of Veterinary Research
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• v.39 no.4
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• pp.797-805
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• 1999

T sergenti cDNA library were constructed to get a more broad information about the structural, functional or antigenic properties of the proteins, and analyzes for their partial cDNA sequences and expression sequences tags(ESTg). The mRNA were purified from T sergenti isolates to identify the information of antigen gene, then first and second strand cDNA was synthesized. EcoR I adaptor ligation and Xho I enzyme restriction were used to the synthesized cDNA, and ligated into a Uni-ZAP XR vector. T sergenti cDNA library was constructed with packaging and amplification in vitro. Antibody screening was performed with constructed T sergenti cDNA library using antisera against T sergenti. Among those clones, eight phagemids were rescued from the recombinant in vivo excision with f1 helper phage. Using the analysis of endonuclease restriction and PCR, the recombinant cDNA were proved having a 0.5-3.0kb of inserts. The eight of partial cDNA clones' sequences were obtained and examined for their homology using BLASTN and BLASTX. The eight of sequenced clones were classified into three groups according to the basis of database searches. A total 3,045bp of partial cDNA sequence were determined from six clones. The putatively identified clones contain a cytochrome c gene, a heat shock protein gene, a cyclophilin gene, and a ribosomal protein gene. The unidentified clones have a homology to ATP-binding protein(mtrA) gene of S argillaceus, DNA-binding protein(DBP) gene of Pseudorabies virus 85kDa merozoite protein gene of B bovis, mRNA spm1 protein of T annulata and glycine-rich RNA-binding protein mRNA of O sativa etc.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.32 no.3
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• pp.249-256
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• 1996

The saury stick - held dip net fishery of Korea in the Northwest Pacific Ocean began in 1985 with 3 vessels. Since then the fishery has grown so rapidly year by year that the number of vessels increased to 34 and the catch to 40 thousands Mff in 1993. In the present study, the monthly situation and the movement of the fishing ground for the pacific saury in the Northwest Pacific were investigated and the monthly optimum fIshing tempertures were estimated, from the relationship between the catch and the sea surface temperature. In the beginning of the fishing season, June to August, the fIshing ground is formed in the very wide range, mainly in the northward area of the front meandering, as the parcific saury migrate northward. In the main season, September to November, the ground is formed in the southward area of the front, off the east coast of Japan. Andd then, the fishing season ends in December with the ground coming near to the east coast of Japan. The monthly optimum fishing tempertures showed 11~$13^{\circ}C.$ in June to July, 13-$17^{\circ}C.$ in August to November, and 15~ $17^{\circ}C.$ in December in general. The profIles of water temperature were much different in accordance by net casting position in the fishing ground, regardless of season. So, the MLD and CPUE was analyzed to find out the relation between the water temperature and the catch. Then, it is found that the CPUE shows over 0.7Mtr/haul as the MLD 40~70m.40~70m.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.21 no.2
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• pp.541-551
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• 1996

This study develops the real time ULTC(Under Load Tap Changer) control strategy with LDC setting values moved dynamically using artificial neural networks. The suggested strategy can improve the ULTC voltage compensation capability by building 2 types of neural networks, ANNs and ANNg. ANNs recognizes the uncompensated MTr sending voltage change caused by the receiving voltage variation. And ANNg dynamically determines the most appropriate ULTC setting valtage chanbe caused by the receiving voltage variation. And ANNg dynamically determines the most appropriate ULTC setting values by recognizing the voltage level obtained from ANNs, and the section load pattern for each time period. In order to evaluate the suggested approach, the ULTC voltage compensation strategy are simulated on a 8 feeder distribution system. Artificial neural networks developed in this study are implemented in FORTRAN language on PC 386.

• Food Science of Animal Resources
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• v.36 no.2
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• pp.223-229
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• 2016

The carcass size performances and the meat quality of Gannan and Sibu yak were determined using M. supraspinatus (SU), M. longissimus thoracis (LT) and M.quadriceps femoris (QF). It is found that Sibu yak had significantly higher carcass weight (CW) than Gannan yak with difference of nearly 40 kg, as well as significantly higher eye muscle area (EMA), carcass thorax depth (CTD), round perimeter (RP), etc. The carcass performances of steer yak were significantly higher than heifer yak except meat thickness at round (MTR) (p<0.05). The results show that both yak breed and gender had significant effects on carcass performances. It could be seen that the variation of carcass size performances from breeds is as large as from gender (50.22% and 46.25% of total variation, respectively) through principal component analysis (PCA). Sibu yak had significantly higher L*, b*, WBSF, cooking loss and Fat content, while Gannan yak had significantly higher a*, press loss, protein content and moisture (p<0.05). Yak gender and muscle had insignificant effects on meat colour and water holding capacity (p>0.05). The variation of meat quality of yak from breed is up to 59.46% of total variation according to PCA. It is shown that the difference between breeds, for Gannan yak and Sibu yak, plays an important role in carcass size performance and meat quality.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38A no.9
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• pp.744-749
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• 2013

We propose soft user data input on LDPC codes with parity encoded by the (1, 7) run length limited (RLL) code for perpendicular magnetic recording channel. The user data are encoded by maximum transition run (MTR) (3;11) code. In order to minimize the loss of code rate, the (1, 7) RLL code only encode the parity of LDPC. Also, to increase performance, we propose only user data part applied soft output Viterbi algorithm (SOVA). The performance using the SOVA showed good performance lower than 26 dB. In contrast, it showed worse performance high than 26 dB. This is because of incorrect soft information by high jitter noise and two different input types for LDPC decoder.

• Nuclear Engineering and Technology
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• v.50 no.8
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• pp.1266-1276
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• 2018

In this paper, three-dimensional neutronic calculations were performed in order to calculate the dependency of CRW on the temperature of fuel and moderator and the moderator void. Calculations were performed using the known MTR_PC computer codes in the core configuration 61 of TRR. The dependency of CRW on the fuel temperature in the range of $20-340^{\circ}C$ and the moderator temperature of each control rods were studied. Based on the positions of the control rods, the calculations were performed in three different cases, named case A, B and C. By the results, the worth of each control rods increases by increasing of the coolant temperature in all methods, however, the total CRW is somewhat independent of the fuel temperature. In addition, the results showed that the variation of CRW versus density depends on the positions of the control rods and the most change in CRW in the coolant temperature, $20-100^{\circ}C$ (279 pcm), belongs to SR4. Finally the effect of void on CRW was studied for different void fraction in coolant. The most worth change is about $2 for 40% void fraction related to SR1 and SR3 in case B. For 40% void fraction, the total CRW increases about $7.5, $6 and $7 in cases, A, B and C, respectively.

• Korean Journal of Environmental Agriculture
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• v.42 no.3
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• pp.253-258
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• 2023

In the environment, chromium often exists in a highly mobile and toxic form of Cr(VI). Therefore, the reduction of Cr(VI) to less toxic Cr(III) is considered an effective remediation strategy for Cr(VI)-contamination. In this study, the biological reduction of hexavalent chromium was examined at the concentrations of 0.01 mM, 0.1 mM, and 1 mM Cr(VI) by the dissimilatory metal-reducing bacterium, Shewanella sp. HN-41 in the presence of ferric-citrate. With the relatively condensed cell densities, the aqueous phase Cr(VI) was reduced at the proportions of 42%, 23%, and 31%, respectively for the 0.01 mM, 0.1 mM, and 1 mM Cr(VI) incubations, while Fe(III)-citrate was reduced at 95%, 88%, and 73%, respectively. Although the strain HN-41 was not considered to reduce Cr(VI) as the sole electron acceptor for anaerobic metabolism in the preliminary experiment, it has been presumed that outer-membrane c-type cytochromes such as MtrC and OmcA reduced Cr(VI) in the presence of ferric-citrate as the electron acceptor. Since this study indicated the potential of relatively high cell density for Cr(VI) reduction, it might propose a bioremediation strategy for Cr(VI) removal from contaminated waters using engineered systems such as bioreactors employing high cell growths.

• Journal of Microbiology and Biotechnology
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• v.34 no.4
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• pp.969-977
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• 2024

Indigo is a valuable, natural blue dye that has been used for centuries in the textile industry. The large-scale commercial production of indigo relies on its extraction from plants and chemical synthesis. Studies are being conducted to develop methods for environment-friendly and sustainable production of indigo using genetically engineered microbes. Here, to enhance the yield of bioindigo from an E. coli whole-cell system containing tryptophanase (TnaA) and flavin-containing monooxygenase (FMO), we evaluated tryptophan transporters to improve the transport of aromatic compounds, such as indole and tryptophan, which are not easily soluble and passable through cell walls. Among the three transporters, Mtr, AroP, and TnaB, AroP enhanced indigo production the most. The combination of each transporter with AroP was also evaluated, and the combination of AroP and TnaB showed the best performance compared to the single transporters and two transporters. Bioindigo production was then optimized by examining the culture medium, temperature, isopropyl β-D-1-thiogalactopyranoside concentration, shaking speed (rpm), and pH. The novel strain containing aroP and tnaB plasmid with tnaA and FMO produced 8.77 mM (2.3 g/l) of bioindigo after 66 h of culture. The produced bioindigo was further recovered using a simple method and used as a watercolor dye, showing good mixing with other colors and color retention for a relatively long time. This study presents an effective strategy for enhancing indigo production using a combination of transporters.

• Investigative Magnetic Resonance Imaging
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• v.10 no.2
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• pp.98-107
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• 2006

Magnetization Transfer (MT) imaging generates contrast dependent on the phenomenon of magnetization exchange between free water proton and restricted proton in macromolecules. In biological materials in knee, MT or cross-relaxation is commonly modeled using two spin pools identified by their different T2 relaxation times. Two models for cross-relaxation emphasize the role of proton chemical exchange between protons of water and exchangeable protons on macromolecules, as well as through dipole-dipole interaction between the water and macromolecule protons. The most essential tool in medical image manipulation is the ability to adjust the contrast and intensity. Thus, it is desirable to adjust the contrast and intensity of an image interactively in the real time. The proton density (PD) and T2-weighted SE MR images allow the depiction of knee structures and can demonstrate defects and gross morphologic changes. The PD- and T2-weighted images also show the cartilage internal pathology due to the more intermediate signal of the knee joint in these sequences. Suppression of fat extends the dynamic range of tissue contrast, removes chemical shift artifacts, and decreases motion-related ghost artifacts. Like fat saturation, phase sensitive methods are also based on the difference in precession frequencies of water and fat. In this study, phase sensitive methods look at the phase difference that is accumulated in time as a result of Larmor frequency differences rather than using this difference directly. Although how MT work was given with clinical evidence that leads to quantitative model for MT in tissues, the mathematical formalism used to describe the MT effect applies to explaining to evaluate knee disorder, such as anterior cruciate ligament (ACL) tear and meniscal tear. Calculation of the effect of the effect of the MT saturation is given in the magnetization transfer ratio (MTR) which is a quantitative measure of the relative decrease in signal intensity due to the MT pulse.

• Journal of Pharmacopuncture
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• v.18 no.4
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• pp.26-31
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• 2015

Objectives: This study used semiology based on traditional Chinese medicine (TCM) to investigate vital energy (Qi) behavior in women with abdominal myofascial pain syndrome (AMPS). Methods: Fifty women diagnosed with chronic pelvic pain (CPP) secondary to AMPS were evaluated by using a questionnaire based on the theories of "yin-yang," "zang-fu", and "five elements". We assessed the following aspects of the illness: symptomatology; specific location of myofascial trigger points (MTrPs); onset, cause, duration and frequency of symptoms; and patient and family history. The patients tongues, lips, skin colors, and tones of speech were examined. Patients were questioned on various aspects related to breathing, sweating, sleep quality, emotions, and preferences related to color, food, flavors, and weather or seasons. Thirst, gastrointestinal dysfunction, excreta (feces and urine), menstrual cycle, the five senses, and characteristic pain symptoms related to headache, musculoskeletal pain, abdomen, and chest were also investigated. Results: Patients were between 22 and 56 years old, and most were married (78%), possessed a elementary school (66%), and had one or two children (76%). The mean body mass index and body fat were 26.86 kg/cm2 (range: 17.7 - 39.0) and 32.4% (range: 10.7 - 45.7), respectively. A large majority of women (96%) exhibited alterations in the kidney meridian, and 98% had an altered gallbladder meridian. We observed major changes in the kidney and the gallbladder Qi meridians in 76% and 62% of patients, respectively. Five of the twelve meridians analyzed exhibited Qi patterns similar to pelvic innervation Qi and meridians, indicating that the paths of some of these meridians were directly related to innervation of the pelvic floor and abdominal region. Conclusion: The women in this study showed changes in the behavior of the energy meridians, and the paths of some of the meridians were directly related to innervation of the pelvic floor and abdominal region.