• Journal of environmental and Sanitary engineering
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• v.14 no.4
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• pp.50-68
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• 1999

The present study was performed to examine on the fauna and standing crops of benthic macroinvertebrates in Pongwha and Youngwol region of upper parts of south han river and Naktons river. The Actual site suvry was caried out on the two times during the April·June to October·November, 1988. Main five areas are Mt. Awrawe(1,067m), Mt. Sontal (1,236m), Mt. Munsu(1,206m), Peak Okyopong(357m) and Mt. Pungnak(760m) area. Total taxa of benthic macroinvertebrates of the study area were 118 species, 45families12 orders, 5 classes in 4 phyla. Occurrence species according to the major taxa of aquatic insects were 35 species (29.66%) in ephemeroptera, 25 species(21.19%) in trichoptera, 25 species(21.19%) hemiptera, 1 species(0.85%) in megaloptera. Non-aqautic insect were 5 species in mollusca, 3 ratio was 8.5 percent. Occurrence species(plecoptera) at clean waters were appeared 12 species at Mt. Awrawe and Sontal area. Occurrence species at each survey area was 67 species at Mt. Awrawe area, 60 species at Mt. Sontal area, 43 species at Mt. Munsu area, 37 species at Mt. P'ungnak area and 34 species at Peak Okyo area, respectively. Species diversity indices were 2.96~3.80 at Mt. Awrawe area, 2.79~3.62 at Mt. sontal area, but 2.64~3.12, 1.59~2.46 and 1.98~2.59 at, Mt. Munsu, P'ungnak and Peak Okyo area, respectively. In this region, occurrence species and individual density were smaller than that of Dong river with good habitat, but those were more abundant than that of Poseong river with similar environment conditions. Therefore, occurrence species and individual density of the present survey region were appeared as somewhat abundant.

• Fisheries and Aquatic Sciences
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• v.13 no.2
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• pp.112-117
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• 2010

Altered mRNA expression of two metallothionein isoforms (MT-IA and MT-IB) in response to acute heavy metal exposure was examined in mud loach, Misgurnus mizolepis, fry using a real-time RTPCR assay. Sublethal exposure (1 or 5 ${\mu}M$) to Cd, Cr, Fe, Mn, Ni, and Zn resulted in highly variable transcriptional responses of the two MT isoforms to the heavy metal ions, including upregulation, a steady state, and downregulation. Overall, the most potent inducer of both MT isoforms was Cd (up to 6-fold). Another exposure experiment using a series of doses of Cu revealed that the stimulation patterns of the two MT isoforms differed: MT-IA transcription was soon saturated at higher concentrations (about 2-fold at 1-4 ${\mu}M$ of Cu), whereas the activation of MT-IB was more dependent on the treatment dose (increased up to 5-fold at 3 ${\mu}M$). The isoform-specific allotment of constitutive and inducible functions was not as clear in fry as in adult tissues. Coordinated interaction between the MT-IA and MT-IB isoforms was hypothesized based on the finding that MT-IA represented a primary action under 'less stressful' or 'sublethal' conditions, whereas the activation of MT-IB became important under 'more stressful' or 'lethal' circumstances in this species.

• Journal of Korean Society of Forest Science
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• v.95 no.2
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• pp.220-231
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• 2006

The study was conducted to investigate the differences in carbon storage of Quercus mongolica stands with respect to latitude and altitude in Korea. Study sites were located in Mt. Joongwang, Pyeongchang-gun, Gangwon-do (altitudes 1,300 m, 1,000 m, and 800 m), Mt. Taehwa, Gwangju-si, Gyeonggi-do (altitude 350 m), Mt. Wolak, lecheon-si, Chungcheongbuk-do (altitude 300 m), Mt. Baekwoon, Gwangyang-si, Jeollanam-do (altitude 800 m), and Mt. Halla, Jeju-do (altitude 1,000 m). Total carbon storage and annual carbon storage of Q. mongolica stands were 85-210 tonC/ha and 7.2-10.6 tonC/ha, respectively. Lower latitude (NE) stands of Q. mongolica showed more carbon storage and annual carbon storage than higher latitude stands. Carbon storage and annual carbon storage of Q. mongolica stands were increased in low altitude. Carbon storage of Q. mongolica stands was higher in the northern aspect than in the southern aspect. However, there were no significant differences in annual carbon storage between the aspects.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.27 no.4
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• pp.314-320
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• 2001

Matrix metalloproteinases have long been viewed as ideal candidates for proteinases that enables tumor cells to permeated basement membrane defenses and invade surrounding tissue. There is growing evidence that the MMPs have an expanded role, as they are important for the creation and maintenance of a microenvironment that facilitates growth and angiogenesis of tumors at primary and metastatic sites. MT-MMPs are not secreted but instead remaining attached to cell surfaces. Although not all of the MT-MMPs are fully characterized, MT-MMPs have important role in localizing and activating secreted MMPs. The MMP genes are transcriptionally responsive to a wide variety of oncogene, growth factors, cytokine, and hormones. Currently, a number of MMP inhibitors are being developed and some have reached clinical trials as anti-metastatic or anti-cancer therapies. MT1-MMP is involved in the activation of proMMP-2. MT1-MMP is significant not only as a tumor marker but as a new target for chemotherapy against cancer. The purpose of this study was to evaluate the effects of protein kinase C inhibitor(genistein) on the proliferation of HT1080 and expression of MT1-MMP mRNA. Human fibrosarcoma cell line HT1080 was cultured and divided 2 groups. The experimental group was treated with $100{\mu}M$ genistein and incubated 12h, 24h for $[3^H]-thymidine$ uptake assay and northern hybridization individually. And the control group was treated with same amount of PBS for the above procedures. $[3^H]-thymidine$ incorporation was measured with ${\beta}$ ray detector. And RT-PCR and northern blotting for MT1-MMP mRNA was performed. The results were as follows 1. $[3^H]-thymidine$ uptake was reduced in experimental group with statistical significance. 2. MT1-MMP mRNA expression was significantly reduced in experimental group. These results showed that protein kinase C inhibitor (genistein) inhibited proliferation of HT1080 and almost completely blocked transcription of MT1-MMP mRNA. So, it is possible to use the protein kinase inhibitor (genistein) as anti-metastatic and anti-proliferative agent.

• Journal of Korean Society of Forest Science
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• v.110 no.4
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• pp.590-600
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• 2021

This study compared the stand structure of Abies koreana forests between Mt. Jirisan and Mt. Hallasan by analyzing DBH distribution, stem vitality, crown structure, importance value, species diversity, and DCA. The data were acquired through the national long-term ecological research project conducted by the Ministry of Environment. In the comparison of DBH class distribution, the stem density of A. koreana with DBH < 20 cm was higher in Mt. Hallasan than that in Mt. Jirisan; however, the stem density of A. koreana with DBH > 20 cm was higher in Mt. Jirisan than that in Mt. Hallasan. For A. koreana stem vitality, the ratio of alive standing was higher in Mt. Jirisan (72.0%) than in Mt. Hallasan (60.7%), whereas the ratio of alive leaning was higher in Mt. Hallasan (10.2%) than in Mt. Jirisan (1.1%). A. koreana stand in Mt. Jirisan was a mature stand with four evenly developed and distinct layers, whereas the tree layer in Mt. Hallasan was less than 10 m and the stand had three layers without a subtree layer. Mt. Hallasan's importance value of A. koreana was 39.4% higher than Mt. Jirisan's importance value of 26.6%. The species diversity of the A. koreana community of Mt. Jirisan was 2.52 times higher than that of Mt. Hallasan, which was 1.58. DCA results revealed that the average distance between Mt. Jirisan and Mt. Hallasan was relatively distinguished, and the degree of scattering of species composition of Mt. Jirisan was relatively dense compared with that of Mt. Hallasan.

• Development and Reproduction
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• v.12 no.3
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• pp.261-266
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• 2008

In the present study, we investigated in vivo effects of Manchurian trout recombinant gonadotrophin hormone (mt-rGTH) on the induction of maturation in female eel, Anguilla japonica. The brood stock, female eel (450$\pm$50 g) were weekly injected intramuscularly with different doses of 0.1, 1, 10 ${\mu}g\m{\ell}$/fish (mt-rFSH or mt-rLH) for 10 week. The effects of r-mtGTH were analyzed by gonadosomatic index (GSI), ovarian follicle diameter and sex steroid levels. All groups did not exhibit significant differences in the GSI values. Whereas plasma testosterone (T) and estradiol-17$\beta$ (E2) levels did not change significantly in control group, plasma levels of T and E2 by injection of the r-mtFSH or r-mtLH were increased at 2 or 4 week after injection. In addition, injection of the mt-rFSH (1, 10 ${\mu}g/m{\ell}$/fish) or mt-rLH (0.1, 1, 10 ${\mu}g/m{\ell}$/fish) significantly increased follicle diameters comparing to the control group. These results demonstrate that the recombinant hormone may affect early ovary development and maturation in female eel. Taken together, these results suggest that the recombinant Manchurian trout FSH and LH are effective for reproductive activities in female eel.

• Tuberculosis and Respiratory Diseases
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• v.50 no.5
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• pp.579-590
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• 2001

Background : The aging process may be induced, at least in part, by reactive oxygen species(ROS). It has been thought that the lung could be a good source of ROS because it has a high oxygen tension. In the present study, we invetigated the inducibility of the first and last lines against oxidative stress, superoxide dismutases(CujZn-SOD and Mn-SOD) as a scavenger of ${O_2}^-\;{\cdot}$ and metallothionein(MT) as a scavenger of $OH{\cdot}$, respectively, in mouse lungs with age. Methods : Oxidative stress was induced by paraquat, an intracellular superoxide generator, at 1, 4, 8, and 12 months of age and then SODs and MT mRNAs were determined by RT-PCR method. Results : The steady-state level of Mn-SOD mRNA increased from 1 to 8 months but decreased thereafter. However, Mn-SOD mRNA was not induced by paraquat after 1 month. On the other hand, there was no change in the steady-state level of Cu/Zn-SOD mRNA, which decreased abruptly at 12 months of age. Additionally, Cu/Zn-SOD mRNA was not induced by paraquat at any age. There was no change in the steady-state level of MT mRNA with age whereas its inducibility by paraquat was intact at all ages. Conclusion : These results indicate that lack of induction of SODs with age may be one of the causative factors in the aging process while induction of MT may play an important role in the defense against oxidative stress. It is therefore implicated that the tissue antioxidant/prooxidant balance could be one of determinants of mean life span.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.1
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• pp.84.1-84.1
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• 2015

The Baptistina family is one of the typical young asteroid families with an age estimated to be about 140-320 Myrs old (Masiero et al. 2012); considered to have not enough time to experience a significant collisional and dynamical evolution since it was formed. Therefore, it may offer a unique insight into spin rate distribution of relatively fresh fragments and physical mechanism of a family break-up event. Observations of the Baptistina family asteroids were conducted during 111 nights from 2013 Oct. to 2015 Feb., using 0.5 m- to 2 m- class telescopes at 6 observatories in the northern hemisphere. We used CCD cameras on the Sobaeksan Optical Astronomy Observatory (SOAO) 0.6 m telescope on Mt. Sobaek, Korea, the Lemmonsan Optical Astronomy Observatory (LOAO) 1.0 m telescope on Mt. Lemmon, USA, the Tubitak Ulusal Gozlemevi (TUG) 1.0 m telescope in Bakirlitepe, Turkey, the Bohyunsan Optical Astronomy Observatory (BOAO) 1.8 m telescope on Mt. Bohyun, Korea, the McDonald Observatory 2.1 m Otto Struve Telescope on Mt. Locke, USA, and the National Astronomical Research Institute of Thailand (NARIT) Observatory 2.4 m telescope on Mt. Doi Inthanon, Thailand. Here, we will present our preliminary results for lightcurve analyses of Baptistina family members.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.16 no.5
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• pp.49-57
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• 2013

This study was carried out to analysis change of distribution and habitat location environment of Korean Fir which is typically vulnerable species by the climate change in Mt. Jiri and Halla. Korean Fir was decreased 18% during 27 year since year 1981, Mt. Halla was 34% during 15 years since year 1988. In the same periods, Temperature change was increased from 8.56 to $9.36^{\circ}C$, from 11.2 to $12.1^{\circ}C$. Distribution changes by the elevation showed higher change ratio 1,400~1,600m in Mt. Jiri and 1,200~1,900m in Mt. Halla. Changes of Korean Fir each slope aspects was high $180{\sim}360^{\circ}$ in Mt. Jiri, $45^{\circ}$ in Mt. Halla. In slope was $30^{\circ}$ in Mt. Jiri and $20^{\circ}$ in Mt. Halla. Changes by reliefs was 12 in Mt. Jiri, 0 or 15 in Mt. Halla, and Sites of Korean Fir was convex slopes both of two areas. Changes by soils was in the good drainage textures.

• The Korean Journal of Zoology
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• v.38 no.1
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• pp.87-95
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• 1995

한국산 담수어류의 잉어목, 황어아과(Leuciscinae) 어류 2속 5종의 계통적 유연관계를 구명하기 위하여 mtDNA분석을 실시하였다 6 base를 인지하는 10개의 제한효소를 처리하여 얻어진 강tDNA의 크기는 16.5-17.5 Kb였으며 Bcl I, Bgl I, Bgl II, Hin dIII, Pvu II, Xba I 등은 종간 차이가 뚜렷하였다. 각종의 집단간 mtDNA분화정도는 매우 낮았으나(p=1% 미만) M. oxyephalus의 무주집단과 제주집단은 예리적으로 큰 차이를 보였다(p=5.3%) Moroco속의 종간 분화정도를 비교한 결과 M. oxycephafus와 M. lagowsk서 사이가 평균 f=7.2%로 근면관계가 제일 가까웠고 M. keumkang과 M. semotilus는 타종들과 근연관계가 제일 멀었다. Moroco속과 Phoxinus속간의 평균 유전적 분화정도는 f=13.7%로 현저한 차이를 보였다. Brown 등(1979)의 공식을 이용하여 이들 황어아과 2속 5종의 분화시기를 추정한 결과 이들은 후기 선신세(Pliocene)와 흥적세(Pleistocene) 사이에 분화된 것으로 추정되었으며 이 결과는 동위효소 연구에서 얻어진 결파(Yang and Min, 1989)와 잘 일치한다.