• Mycobiology
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• 제29권1호
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• pp.43-47
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• 2001

Genetic diversity of 21 Korean Phytophthora capsici isolates was analyzed by using several molecular markers such as random amplified polymorphic DNA(RAPD), M-13, microsatellite and random amplified microsatellite sequences(RAMS). The overall average similarity coefficient among the isolates was 86% based on the combined data obtained by the molecular markers. No molecular markers were found to be associated with hosts or geographic regions. In addition to RAPD, analysis based on repeated sequences such as $(GTG)_5$, M-13 and RAMS could be used to assess population structure of P. capsici.

• Plant Resources
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• 제5권3호
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• pp.169-175
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• 2002

The soybean cyst nematode (Heterodera glycines Inchinoe; SCN) is a devastating pest of soybean and is responsible for significant losses in yield. The use of resistant cultivars is the effective method to reduce or eliminate SCN damage. The objective of this research is to identify AFLP markers linked to the SCN resistant genes. Bulked genomic DNA was made from resistant and susceptible genotypes to SCN and a total of 19 primer combinations were used. About 31 fragments were detected per primer combination. The banding patterns were readily distinguished in resistant and susceptible bulked genotypes. Polymorphic fragments were detected between resistant and susceptible bulked genotypes in the primer combination of CGT/GGC, CAG/GTG and CTC/GAG. In primer combinations of CGT/GGC and CAG/GTG, bulked resistant genotype produced a polymorphic bands. However, in primer of CTC/GAG, bulked susceptible genotype produced a polymorphic fragments. Three AFLP markers identified as a polymorphic fragments between bulked genomic DNA were mapped in 85 F2 population. Among them, only two markers, CGT/GGC and CTC/GAG, was linked and was mapped. Broad application of AFLP marker would be possible for improving resistant cultivars to SCN.

• 한국작물학회지
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• 제57권1호
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• pp.71-77
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• 2012

The objective of this study is to develop the gene based sequence tagged site (STS) markers in wheat. The euchromatin enriched genomic library was constructed and the STS primer sets were designed using gene based DNA sequence. The euchromatin enriched genomic (EEG) DNA library in wheat was constructed using the $Mcr$A and $Mcr$BC system in $DH5{\alpha}$ cell. The 2,166 EEG colonies have been constructed by methylated DNA exclusion. Among the colonies, 606 colonies with the size between 400 and 1200 bp of PCR products were selected for sequencing. In order to develop the gene based STS primers, blast analysis comparing between wheat genetic information and rice genome sequence was employed. The 227 STS primers mainly matched on $Triticum$ $aestivum$ (hexaploid), $Triticum$ $turgidum$ (tetraploid), $Aegilops$ (diploid), and other plants. The polymorphisms were detected in PCR products after digestion with restriction enzymes. The eight STS markers that showed 32 polymorphisms in twelve wheat genotypes were developed using 227 STS primers. The STS primers analysis will be useful for generation of informative molecular markers in wheat. Development of gene based STS marker is to identify the genetic function through cloning of target gene and find the new allele of target trait.

• 생명과학회지
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• 제30권3호
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• pp.239-249
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• 2020

감 꽃은 과실을 맺는 암꽃과 꽃가루를 내는 수꽃, 암술과 수술을 모두 가진 양성화가 있다. 관행 교배육종법으로 부본용 수꽃 신품종을 육성한다면 최종 선발까지 15년 정도의 장기간과 넓은 시험포장이 요구된다. 유묘기에 수꽃 착생 개체를 조기에 선발하여 육종효율을 증진하고자 꽃의 성표현을 알고 있는 주요 품종 88개체를 대상으로 Akagi 등(2014)이 보고한 수꽃 선발 마커들의 적용 효과를 검증하였다. OGI locus marker와 DISx-AF4S marker 적용 결과 각각 83품종, 72품종에서 꽃의 성표현과 분석 결과가 일치하였다. 육성품종인 '미감조생'을 모본으로 한 교배조합 등으로부터 나온 실생 2,509개체에 OGI locus marker 수꽃 선발 마커를 적용하여 890개체를 선발하였고, 분석 완료한 교배실생 중 아직 착과가 되지 않았거나 고사한 개체를 제외한 1,186개체를 대상으로 꽃의 성표현과 마커 분석 결과를 비교하였을 때 401개체가 불일치한 것을 확인하였다(33.8%). 다양한 교배조합으로부터 나온 실생 889개체에 DISx-AF4S marker를 적용하여 분석한 결과 636개체를 선발하였다. 분석 완료한 교배실생 중 아직 착과가 되지 않았거나 고사한 개체를 제외한 379개체를 대상으로 꽃의 성 표현과 마커 분석 결과를 비교하였고 247개체가 불일치한 것을 확인하였다(65.2%). 이상의 결과들을 종합하여 볼 때, 검토한 DISx-AF4S, OGI locus marker는 육종현장에서 활용하기에 유용하지 않을 것으로 판단된다.

• 한국작물학회지
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• 제44권4호
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• pp.345-349
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• 1999

This study was carried out to map Bphl and bph2 gene in Mudgo and Sangju13 (Oryza sativa L.) respectively conferring resistance to brown plan-thopper (BPH) and to establish the marker-assisted selection (MAS) system. Bulked seedling (grown for 20 days) test was conducted with the 73 F4 lines derived from a cross between Nagdongbyeo and Mudgo for Bphl and with 53 BC3F5 lines derived from the Milyang95/Sangju13 cross for bph2. Bph1 was mapped between RG413 and RG901 on chromo-some 12 at a distance of 7.5 cM from RG413 and 8.4 cM from RG90l. A recessive gene bph2 was located near RZ76 on chromosome 12 at a distance of 14.4 cM. Bphl and bph2 were linked to each other with a distance of about 30 cM. An RFLP marker, RG413 linked to Bphl, was converted to an STS marker to facilitate the marker-assisted selection. BPH resistant genotypes could be selected with 92% accuracy in a population derived from a line of NM47-B-B.

• 대한본초학회지
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• 제31권5호
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• pp.15-23
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• 2016

Objectives : Araliae Continentalis Radix and Angelicae Pubescentis Radix have been used as the same medicinal name Korean and Chinese traditional medicines, respectively. The authentic Araliae Continentalis Radix is described only the root of Aralia continentalis in the Korean Pharmarcopoeia. However, the dried root of Angelica biserrata, Levisticum officinale, or Heracleum moellendorffii also has been distributed adulterants of Araliae Continentalis Radix. To develop a reliable method for identifying Araliae Continentalis Radix from adulterants, we carried out the analyses of universal DNA barcode sequences.Methods : Four plants species were collected from different habitate and nucleotide sequences of matK and rbcL were analyzed. The species-specific sequences and phylogenetic relationship were estimated using entire sequences of two DNA barcodes, respectively.Results : In comparative analysis of matK sequences, we were identified 104 positions of marker nucleotide for Ar. continentalis, 3 for An. biserrata, 4 for L. officinale and 8 for H. moellendorffii enough to distinguish individual species, respectively. Furthermore, we obtained marker nucleotides in rbcL at 42 positions for Ar. continentalis, 5 for An. biserrata and 2 for H. moellendorffii, but not for L. officinale. The phylogenetic tree of matK and rbcL were showed that all samples were clustered into four groups constituting homogeneous clades within the species.Conclusions : We confirmed that species-specific marker nucleotides of matK sequence provides distinct genetic information enough to identify four species. Therefore, we suggest that matK gene is useful DNA barcode for discriminating authentic Araliae Continentalis Radix from inauthentic adulterants.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2004년도 The 2004 KSPP Annual Meeting & International Symposium
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• pp.52.1-53.1
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• 2004

See Full Text

• 한국작물학회:학술대회논문집
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• 한국작물학회 1996년도 춘계 학술대회지
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• pp.26-27
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• 1996

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2009년도 심포지엄 및 춘계학술발표회
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• pp.105-106
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• 2009

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 춘계학술대회
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• pp.97-97
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• 2022