• Proceedings of the Korean Society of Organic Agriculture Conference
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• 2002.12a
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• pp.74-82
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• 2002

난용성 인산염을 가용화 시키는 Penicillium sp. PS-113와 Lactobacillus sp. 를 이용하여온실에서 오이에 대한 생육 실험을 실시하였다. 실험 결과는 다음과 같다. Penicillium sp. PS-113 $5.0{\times}106cfu/ml$ + Lactobacillus sp. $6{\times}106cfu/ml $동시 처리구에서 평균 오이 길이는 대조구에 비하여 3cm 더 긴것으로 조사되었고, 오이 낱개의 무게는 310.6g으로 무처리구에 비하여 89.2g 이 증가하였으며, 오이 전체 생산량은 10681.7g로 무처리구와 비교해 볼 때 4517.3g이 많아 생산량이 73% 증가하였다. Penicillium sp. PS-113 $5.0{\times}105cfu/ml$ 단독처리와 Penicillium sp. PS-113 $5.0{\times}105cfu/ml$ + Lactobacillus sp. $6{\times}106cfu/ml$의 동시 처리구도 오이의 평균 무게와 길이에 있어 무처리구보다 약간씩 더 높았다. Penicillium sp. PS-113 $5.0{\times}105cfu/ml$ + Lactobacillus sp. $6{\times}106cfu/ml$의 동시처리구는 무처리에 비하여 오이의 수가 2개 적었으나 오이 전체 생산량은 무처리구에 비하여 Penicillium sp. PS-113 $5.0{\times}105cfu/ml$ 단독 처리구에서 53.7%, Penicillium sp. PS-113 $5.0{\times}104cfu/ml$ + Lactobacillus sp. $6{\times}106cfu/ml$ 동시 처리구에서 48.5% 증가되었다. 따라서 인산가용화균의 처리가 오이의 생산성을 증대시키는 것으로 나타났다.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.470-473
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• 2000

Since the biosynthetic production of indigo and indirubin normally reflects a difficult process including the toxicity of indole to microorganisms, only several bacterial strains have been exploited to produce indigo and indirubin from indole or its derivatives. P. savastanoi BCNU 106, which was a gram negative bacterium, was isolated and tolerant to 10% (v/v) toluene. The indole tolerance level of P. savastanoi BCNU 106 was as high as 160 mg/ml when toluene or p-xylene was added to the medium to 20% by volume. P. savastanoi BCNU 106 grown in a two-phase culture system containing indole(100 mg/ml) and P-xylene (0.2 ml/ml) produced P-xylene-soluble pigments, blue indigo and purple indirubin. Of the conditious tried, the production of indigo and indirubin was found only when P. savastanoi BCNU 106 was grown in the two-phase system overlaid with the organic solvents with appropriate polarity. This study may illustrate that the isolated extremophile P. savastanoi could be used in the microbial conversion process of the industrial potentials.

• Korean Journal of Animal Reproduction
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• v.10 no.2
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• pp.204-210
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• 1986

As a part of in vitro fertilization(IVF) for farm animals, IVF experiment was conducted using New Zealand white rabbits with their sperm capacitated in vivo. The effect of uterine conditions on sperm capacitation and effect of sperm concentration and fertilization media on IVF rate and implantation of in vitro fertilized ova were studied. The results obtained are summarized as follows; 1. Acrosomal reaction, noted after staining, of sperm recovered from ligated and intact uterus of capacitators was 83.0% and 65.7%, respectively. 2. IVF rate of ova inseminated with sperm from ligated uterus tended to be higher in DM or with higher concentration of sperm than in the modified F12 medium or with lower sperm concentration. Cleavage rate of fertilized ova for 48hr in DM was 31.5% for 106/ml and 30.0% for 104/ml of sperm and that in modified F12 medium was 26.0% for 106/ml and 22.3% for 104/ml of sperm. 3. Using the sperm from intact uterus, cleavage rate of fertilized ova showed same tendency as those shown with ligated uterus. The rate was 82.0% for 106/ml and 66.5% for 104/ml of sperm in DM and was 69.0% for 106/ml and 56.5% for 104/ml of sperm in the medium. 4. When normal ova up to 48hr after IVF were cultured for 4 days in either DM or modified F12 medium, ova developed to blastocyst stage showed higher rate in the groups of higher sperm concentration in the both media. The rate was 80.9% and 60.0% for 106/ml and 104/ml of sperm in DM and 91.7% and 71.4% for 106/ml and 104/ml of sperm in the modified F12 medium, respectively. 5. Rate of implantation after transfer of 4- or 8-cell embryos was 36.8%.

• Mass Spectrometry Letters
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• v.7 no.3
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• pp.69-73
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• 2016

High-resolution quadrupole-Orbitrap mass spectrometry (HRMS), with high-resolution (> 10,000 at full-width at half-maximum) and accurate mass (< 5 ppm deviation) capabilities, plays an important role in the structural elucidation of drug metabolites in the pharmaceutical industry. ML106, a derivative of imidazobenzimidazole, decreased melanin content and tyrosinase activity in a dose-dependent manner. Here, we investigated the phase 1 metabolic pathway of ML106 using HRMS in human liver microsomes (HLMs) and recombinant cDNA-expressed cytochrome P450 (CYP). After the incubation of ML106 with pooled HLMs and recombinant cDNA-expressed CYP in the presence of NADPH, five phase 1 metabolites, including three mono-hydroxylated metabolites (M1-3) and two di-hydroxylated metabolites (M4 and M5), were investigated. The metabolite structures were postulated by the elucidation of protonated mass spectra using HRMS. The CYP isoforms related to the hydroxylation of ML106 were studied after incubation with recombinant cDNA-expressed CYP. Here, we identified the phase 1 metabolic pathway of ML106 induced by CYP in HLMs.

• Journal of Embryo Transfer
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• v.9 no.3
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• pp.249-254
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• 1994

This study was undertaken to investigate effects of granulosa cells on mejotic maturation of porcine oocytes in vitro. The results obtained in this study were summarized as follows : The germinal vesicle breakdown(GVBD) rates were 91.5, 93.3 and 96.6%, respectively, when the cumulus oocy:e cornplexes(COC) in the TCM-199 medium with sodium bicarbonate, Na pyruvate, penicillin G, streptomycin sulfate and 10% FCS were cultured in the condition of FSH(0.02 Au/ml), LH(10 $\mu$g/ml) and FSH + LH added. And when the COC were co-cultured with granulosa cell (5$\times$ 106 cells /ml) in the condition of FSH, LH and FSH + LH added, GVBD rates were 94.3, 92.9 and 98.9%, respectively. However, when the COC were cultured in the condition of hormone free and co-cultured with granulosa cells in the condition of hormone free, the GVBD rates were 40.4 and 86.3%, respectively. The GVBD rates were 41.0, 62.7, 84.6, 88.1 and 93.6%, respectively, when the COC were co-cultured with granulosa cells that the concentrations are 0 cells /ml, 1 $\times$ 106 cells /ml, 5:: 106 cells /ml, 1$\times$ 107 cells /ml and 5$\times$ 107 cells /ml.

• Journal of Embryo Transfer
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• v.6 no.1
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• pp.25-32
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• 1991

The present study was performed to investigate the effects of co-culture with granulosa cells on in vitro fertilization and cleavage of early bovine embryo development. Bovine oocytes were matured for 20-24 hrs in vitro with granulosa cells or without and then fertilized in vitro using frozen-thawed spermatozoa treated with BO-caffeine, BO-BSA(2OmM heparin added). At l8hrs after insemination, oocytes were fixed and examined or further cultured in TCM 199 for 48hrs. The fertilization rates between the control(70.4%) and the groups of co-cultured with granulosa cell(2.5$\times$106 cells/ml; 71.6%, 5.0$\times$ 106/ml; 71.9%, l.0$\times$ 107/ml; 71.1%) did not differ significantly. The cleavage rates in the groups co-cultured with granulosa cell(2.5$\times$ 106 cells/mi; 43.6%, 5.0$\times$ 106/ml; 46.8%. l.0$\times$ 107/ml; 45.0%)were significantly higher than that of without granulosa cell, respectively(P<0.05). However there were no significant differences between the groups co-cultured with granulosa cells. The result indicated that co-culture with granulosa cell was effective means to cleavage of bovine follicular oocytes but did not affect the in vitro fertilization.

• The Korean Journal of Zoology
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• v.33 no.3
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• pp.310-321
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• 1990

To establish the in vitro culture system and quantitation for chondrogenesis, and to investigate the relationship between cell aggregation and chondrogenesis, chick limb bud mesenchymal cells of Hamburger-Hamilton stage 23/24 were micromass cultured in various cell densities. The chondrogenesis was assayed based on checking the alcian blue-stained nodule numbers, the amount of alcian blue extraded, the change in cell numbers, the rate of [35 S] sulfate incorporation and expression of type II collagen. Mesenchymal cells plated with an initial density of high (1 x 107 cells/ml)- and intermediates (5. $\times$ 106 cells/ml)-density were differentiated into cartilage. On the other hand, the cells of low density (2 x 106 cells/mi, 5 $\times$ 105 cells/ml) of stage 23/24 cells and the stage 18/19 cells in three kinds of cell density did not differentiate into cartilage even though the cells formed an aggregated core at the center of cultured mass. From these results and others obtained in this study, it can be stated that the stage 23/24 mesenchymal cells are likely to pass over the aggregation step and have the potentiality to differentiate into chondrocytes. Thus chondrogenesis in vitro can be observed when mesenchymal cells are plated over the threshold density of 5 $\times$ 106 cells/ml. Hyaluronidase (HAase) activity was relatively constant throughout the culture, suggesting that the role of HAase may not be important for the cells of stage 23/24.

• The Korean Journal of Food And Nutrition
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• v.9 no.1
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• pp.21-28
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• 1996

This study was attempted to investigate the production of L-tyrosine by Brevibacterium flavum ATCC 14067. To select the strain which produce more L-tyrosine, mutants were induced by N-methyl-N'-nitro-nitrosoguanidine (NTG) treatment and phenylalanine auxotrophic mutants were induced by NTG and penicillin treatments. PFP resistant mutant was isolated from a phenylalanine auxotroph by retreatment with NTG and screened for increase of L-tyrosine production. PFP-326 mutant resistant to PPP (100ug/ml) was derived from phenylalanine auxotroph by mutagenesis with NTG and PFP-106 mutant resistant to PFP (1201g/ml) was derived from PFP-326 by mutagenesis with NTG. The composition of media for L-tyrosine production in strain PFP-106 was studied. PFP-106 mutant strain produced 50mg 11 of L-tyrosine while the parent strain produced 0.56mg 11 of L-tyrosine. The optimum composition of medium for L-tyrosine by strain PFP-106 was 10cA sucrose as carbon source, 3% ammonium sulfate as nitrogen source. The optimum cultural condition for producing L-tyrosine by strain PFP-106 was L-phenylalanine at a concentration of 1000g/mg.

• KSBB Journal
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• v.5 no.4
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• pp.365-371
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• 1990

To improve the growth of mouse hybridoma 2c3.1 secreting anti-Hepatitis B surface antigen monoclonal antibody (anti-HBsAg MAb), we had constructed an enriched medium and observed the effects of fetal bovine serum and serum-free supplements including human serum albumin, 'insulin and transferrin', and monoethanolamine. For further enhancement of growth, the concentrations of two major energy sources, glucose and glutamine, were strengthened with various ratios in the enriched medium. Maximum cell growth and monoclonal antibody production obtained in various ratios of glucose/glutamine with an inoculation concentration of 2$\times$105 cells/ml were 0.73$\times$106-4.62$\times$106 cells/ml and 65.1-422.6 $\mu\textrm{g}$/ml, respectively. Glutamine was round to be a major energy source and a limiting nutrient in comparison to glucose for 2c3.1 cell cultivation in enriched media with low serum.

• Journal of dental hygiene science
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• v.2 no.1
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• pp.15-19
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• 2002

In this study, we investigated the cytotoxicity of ethyl acetate subfraction of Sophora flavescens Ait.(EASS) on cancer cells using MTT quantitative analysis. The EASS was cytotoxicity from the concentration of 6.25 g/ml to KB, B16, HeLa, and MCF-7 cancer cells and the cytotoxicity was significant, (p < 005) increased as the concentrations of EASS were increased, (12.5, 25, 50, 100 g/ml). The IC for KB, B16, HeLa, and MCF-7 were 56.58, 65.43, 83.95, and 106.65 g/ml, respectively. Conclusively, the EASS inhabited the growth of cancer cells and the order of potency of cytotoxicity was KB > B16 > HeLa > MCF-7.