• 건축사
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• 통권528호
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• pp.66-69
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• 2013

• 건축사
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• 통권573호
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• pp.120-133
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• 2017

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.65-70
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• 2005

A growing body of evidence shows that nitric oxide $({\cdot}NO)$ and ${\cdot}NO-derived$ reactive nitrogen species (RNS) act as both plant physiological regulators and stressors. However, very little is known concerning metabolism of RNS in plant cells. In this paper, we explore a plant metabolic basis for RNS, with special emphasis on the possible relationship to nitrogen assimilation, and discuss the potential of the metabolic engineering for plant-biotechnological application.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.356-360
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• 2005

Xanthine dehydrogenase (XDH), a classic enzyme involved in purine catabolism, can catalyze the formation of redox-signaling reactive oxygen and nitrogen species such as superoxide and nitric oxide. We generated transgenic plants of Arabidopsis in which XDH was knocked out by introduction of hairpin RNA-expression vector. Expression analysis by reverse transcription-PCR and in-gel staining of XDH activity revealed that transgenic lines efficiently suppressedXDH expression at the transcriptional level, demonstrating that RNA interference was successfully induced. XDH-suppressed transgenic lines exhibitedincreased biomass production during the growth of seedlings.

• 한국산학기술학회논문지
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• 제18권10호
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• pp.785-793
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• 2017

미사강변도시 단독주택지에 적용되는 지구단위계획지침이 지역의 특성을 고려하지 않은 부분과 기존의 자치조례에 준하고 있어 이로 인하여 단독주택지의 환경을 악화시키고 있는 것으로 조사되었다. 조사결과 1)미사강변도시의 경우 지역 내의 공동주택(아파트)에 대한 디자인 가이드라인은 디테일하게 규정하고 있는 반면에 단독주택지의 주택건축에 대한 디자인 가이드라인이 계획되어 있지 않다. 2)공동주택과 다가구주택에 대한 주차대수 산정에 대한 형평성과 다가구주택이 단독주택으로 분류됨에 따른 주차장 법규의 미비로 인한 문제로 주거환경이 악화되고 있다. 3)지구단위계획지침에 따라 1층 근생의 외벽재료를 유리로 규정하고 있고 협소한 주차공간으로 유리외벽에 인접하여 주차하는 경우 위험성에 대한 대비가 필요하다. 4)초등학교, 유치원이 위치하고 있음에도 불구하고 주차부족 등 차량의 혼잡도가 높아 통학 학생들에게 많은 위험성이 있다. 5)주차장 넓이를 고려하지 않은 가구계획과, 지역의 특성을 고려하지 않은 주차장법 적용으로 여러 문제점이 발생하고 있다. 6)보행자 전용도로가 보행자의 동선을 고려하지 않고 계획되어 타용도로 전용되고 있다.

• 한국환경복원기술학회지
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• 제10권3호
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• pp.60-70
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• 2007

Green space infrastructures for three cities, Gwacheon, Uiwang, and Hanam, were analyzed in terms of the area of urban parks per capita, the percentage of green space area, the area of green space per capita, and the percentage of vegetation cover in residential area, etc., which are commonly used as criteria for urban green space planning. The differences in green space infrastructure among these three cities were compared to the satisfaction level of residents for their green space. The area of parks per capita corresponded to the satisfaction level when Seoul Great Park in Gwacheon and Misa Park in Hanam were not included. Although these two huge parks accounted more than 90% of the area of urban parks in Gwacheon and Hanam, they serve more people from outside the cities and not likely visited by residents due to lacking of daily accessibility. The percentage of vegetation cover in residential area were considered to affect the satisfaction of residents for green space, whereas the total area of green space or the percentage of green space area in the cities was not related to the satisfaction level. It suggests that the distributions and accessibilities of green space and park service are more important for satisfaction than total green space area indicating urban sustainability.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.57-57
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• 2017

Chrysanthemum is one of the most important floral crop in Korea produced about 7 billion dollars (1 billion for pot and 6 billion for cutting) in 2013. However, it is difficult to breed and to do genetic study because 1) it is highly self-incompatible, 2) it is outcrossing crop having heterozygotes, and 3) commercial cultvars are hexaploid (2n = 6x = 54). Although low-density genetic map and QTL study were reported, it is not enough to apply for the marker assisted selection and other genetic studies. Therefore, we are trying to make high-density genetic mapping using GBS with about 100 $F_1s$ of C. boreale that is oHohhfd diploid (2n = 2x = 18, about 2.8Gb) instead of commercial culitvars. Since Chrysanthemum is outcrossing, two-way pseudo-testcross model would be used to construct genetic map. Also, genotype-by-sequencing (GBS) would be utilized to generate sufficient number of markers and to maximize genomic representation in a cost effective manner. Those completed sequences would be analyzed with TASSEL-GBS pipeline. In order to reduce sequence error, only first 64 sequences, which have almost zero percent error, would be incorporated in the pipeline for the analysis. In addition, to reduce errors that is common in heterozygotes crops caused by low coverage, two rare cutters (NsiI and MseI) were used to increase sequence depth. Maskov algorithm would also used to deal with missing data. Further, sparsely placed markers on the physical map would be used as anchors to overcome problems caused by low coverage. For this purpose, were generated from transcriptome of Chrysanthemum using MISA program. Among those, 10 simple sequence repeat (SSR) markers, which are evenly distributed along each chromosome and polymorphic between two parents, would be selected.

• Asian Pacific Journal of Cancer Prevention
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• 제13권5호
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• pp.1779-1782
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• 2012

Background & Objectives: In patients with gastric cancer, the most frequently reported family history of cancer also involves the stomach. The aim of this study was to assess the presence of gastric precancerous lesions in first-degree relatives of patients with gastric cancer and to compare the obtained results with those of individuals with no such family history. Methods: Between 2007 and 2009, 503 consecutive persons more than 30 years old were enrolled in the study covering siblings, parents or children of patients with confirmed adenocarcinoma of stomach. The control group was made up of 592 patients who were synchronously undergoing upper gastrointestinal endoscopy for evaluation of dyspepsia without gastric cancer or any family history. All subjects were endoscopically examined. Results: The overall prevalence of Helicobacter pylori was 77.7% in the cancer relatives and in 75.7% in the control group. Chronic gastritis was found in 90.4% vs. 81.1% (P<0.001). Regarding histological findings, 37(7.4%) of the study group had atrophy vs. 12(1.7%) in the control group (P<0.001), while no difference was observed for intestinal metaplasia (20.3%vs. 21.6%, P=0.58). Dysplasia were shown in 4% of cancer relatives but only 0.4% of the control group (P<0.001). There was no gender specificity. Conclusions: Findings of our study point to great importance of screening in relatives of gastric cancer patients in Iran.

• Clinics in Shoulder and Elbow
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• 제24권2호
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• pp.80-87
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• 2021

Background: Complex regional pain syndrome (CRPS)-related hand lesions are one of the complications following arthroscopic rotator cuff repair (ARCR). This study aimed to investigate the clinical outcomes of patients with CRPS-related hand lesions following ARCR. Methods: Altogether, 103 patients with ARCR were included in this study (mean age, 63.6±8.2 years; 66 males and 37 females; follow-up period, preoperative to 12 months postoperative). Clinical assessment included the Japanese Orthopaedic Association (JOA) score, University of California, Los Angeles (UCLA) score, Constant score, 36-item short form health survey (SF-36) score, and Quick Disabilities of the Arm, Shoulder, and Hand (QuickDASH) score from preoperative to 12 months postoperatively. The patients were either assigned to the CRPS group or non-CRPS group depending on CRPS diagnosis until the final follow-up, and clinical outcomes were then compared between the groups. Results: Of 103 patients, 20 (19.4%) had CRPS-related hand lesions that developed entirely within 2 months postoperatively. Both groups showed significant improvement in JOA, UCLA, and Constant scores preoperatively to 12 months postoperatively (p<001). Comparisons between the two groups were not significantly different, except for SF-36 "general health perception" (p<0.05) at 12 months postoperatively. At final follow-up, three patients had residual CRPS-related hand lesions with limited range of motion and finger edema. Conclusions: CRPS-related hand lesions developed in 19.4% of patients following ARCR. Shoulder or upper-limb function improved in most cases at 12 months, with satisfactory SF-36 patient-based evaluation results. Patients with residual CRPS-related hand lesions at the last follow-up require long-term follow-up.

• Genomics & Informatics
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• 제20권3호
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• pp.35.1-35.9
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• 2022

Microsatellites or simple sequence repeats are motifs of 1 to 6 nucleotides in length present in both coding and non-coding regions of DNA. These are found widely distributed in the whole genome of prokaryotes, eukaryotes, bacteria, and viruses and are used as molecular markers in studying DNA variations, gene regulation, genetic diversity and evolutionary studies, etc. However, in vitro microsatellite identification proves to be time-consuming and expensive. Therefore, the present research has been focused on using an in-house built java pipeline to identify, analyse, design primers and find related statistics of perfect and compound microsatellites in the seven complete genome sequences of coronavirus, including the genome of coronavirus disease 2019, where the host is Homo sapiens. Based on search criteria among seven genomic sequences, it was revealed that the total number of perfect simple sequence repeats (SSRs) found to be in the range of 76 to 118 and compound SSRs from 01 to10, thus reflecting the low conversion of perfect simple sequence to compound repeats. Furthermore, the incidence of SSRs was insignificant but positively correlated with genome size (R² = 0.45, p > 0.05), with simple sequence repeats relative abundance (R² = 0.18, p > 0.05) and relative density (R² = 0.23, p > 0.05). Dinucleotide repeats were the most abundant in the coding region of the genome, followed by tri, mono, and tetra. This comparative study would help us understand the evolutionary relationship, genetic diversity, and hypervariability in minimal time and cost.