• 한국약용작물학회지
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• 제22권6호
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• pp.504-509
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• 2014

Methicillin-resistant Staphylococcus aureus (MRSA) is a substantial contributor to morbidity and mortality. In search of a natural products capable of inhibiting this multidrug resistant bacteria, we have investigated the antimicrobial activity of brazilein (BRZ) isolated from Caesalpinia sappan L. (Leguminosae) against 8 different strains of Staphylococcus aureus (S. aureus). New antimicrobial activity was found using the minimum inhibitory concentrations (MICs), broth dilution as well as checkerboard method. Against the 8 strains, the minimum inhibitory concentrations of BRZ were in the range of $62.5-500{\mu}g/mL$. From those results we performed the checkerboard test to determine the synergism of BRZ in combination with Hygromycin-b (HgB) against 4 strains. The combined activity of BRZ and HgB against 4 strains resulted in a fractional inhibitory concentrations index (FICI) ranging from 0.18-0.5. The effect of BRZ with HgB was found to be synergistic. We found that BRZ reduced the MICs of HgB. BRZ and HgB could lead to the development of new combination antibiotics against MRSA infection.

• 한국통신학회논문지
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• 제36권8A호
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• pp.712-722
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• 2011

Wireless body area network (WBAN)은 인체 주변 영역에서의 통신서비스를 제공한다. WBAN 서비스는 인체 내부에 이식된 의료 응용을 위한 MICS 주파수 대역과 의료 응용과 consumer electronics (CE) 응용 분야 모두를 제공할 수 있는 ISM 주파수 대역에서의 서비스로 이루어지기 때문에 WBAN을 위한 MAC 프로토콜은 의료 응용과 CE 응용간의 상이한 특징과 유연성 (flexibility)을 고려하여 설계되어야 한다. 본 논문에서는 WBAN MAC 프로토콜의 요구사항을 확인하고, WBAN의 요구사항을 만족하는 WBAN MAC 프로토콜을 제안한다. WBAN의 다양한 응용을 위한 전송 유연성을 제공하기 위해서 동적 CFP(Contention Free Period) 할당(Dynamic CFP Allocation)을 제안한다. 또한, 경쟁 기반의 긴급 의료 데이터를 발생하는 의료 응용과 때때로 대량의 데이터를 발생하는 CE 응용을 지원하기 위해서 OCDP(opportunistic contention decision period) 구간과 4-mode Opportunity period를 제안하고, 제안한 방안을 이용하여 Inactive period와 Opportunity period를 일시적으로 전환하여 사용할 수 있는 기법을 제안한다. 다양한 시뮬레이션 결과 IEEE 802.15.4 MAC 프로토콜과 제안하는 WBAN MAC 프로토콜을 비교하였을 때, WBAN 환경에서의 전송 처리량, CFP 이용율, 전송지연 측면에서 증가된 성능 결과를 얻을 수 있었다.

• Preventive Nutrition and Food Science
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• 제21권3호
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• pp.197-201
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• 2016

Helicobacter pylori infection is associated with an increased risk of developing upper gastrointestinal tract diseases. However, treatment failure is a major cause of concern mainly due to possible recurrence of infection, the side effects, and resistance to antibiotics. The aim of this study was to investigate the activities of Centella asiatica leaf extract (CAE) against H. pylori both in vitro and in vivo. The minimum inhibitory concentrations (MICs) against 55 clinically isolated strains of H. pylori were tested using an agar dilution method. The MICs of CAE ranged from 0.125 mg/mL to 8 mg/mL, effectiveness in inhibiting H. pylori growth was 2 mg/mL. The anti-H. pylori effects of CAE in vivo were also examined in H. pylori-infected C57BL/6 mice. CAE was orally administrated once daily for 3 weeks at doses of 50 mg/kg and 250 mg/kg. CAE at the 50 mg/kg dose significantly reduced H. pylori colonization in mice gastric mucosa. Our study provides novel insights into the therapeutic effects of CAE against H. pylori infection, and it suggests that CAE may be useful as an alternative therapy.

• 약학회지
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• 제41권1호
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• pp.132-138
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• 1997

The antimicrobial activity of Manuka oil, a steam distillate from Leptospermum scoparium, was investigated, and it's MIC against ten kinds of microorganisms was determin ed. MICs against bacteria and fungi were measured by means of both two-fold dliution method and agar plate two-fold dilution method, respectively. MICs of Manuka oil against Staphylococcus aureus KCTC 1916 and Micrococcus luteus KCTC 1915, gram-positive microrganisms, were identical as 3.05 ${\mu}$g/ml, while it's antibacterial activity against gram-negative microrganisms such as Pseudomonas aeruginosa KCTC 2513, Escherichia coli KCFC 1682, Klebsiella pneunioniae KCTC 2001 or Proteus vulgaris KCTC 2433 was negligible(MIC: ${\geq}$ 1000 ${\mu}$g/ml), suggesting a high susceptibility of gram-positive bacteria to Manuka oil. In addition, MIC against Aspergillus niger KCTC 6077 was 24 ${\mu}$g/ml. and that against the other fungi, Tricophyton mentagrophytes KCTC 1374 and Candida albicans KCTC 1940 was ${\geq}$ 1000 ${\mu}$g/ml. When Manuka oil ointment was used in combination with other drugs. i.e.. gentamycin sulfate, chlotrimazol and hydrocortisone acetate, and diphenhydramine HCl and hydrocortisone acetate. it's antibacterial activity against Staphylococcus aureus KCTC 1916 was higher than Manuka oil ointment or other drugs alone. In conclusion, Manuka oil possesses a selective antibacterial activity against Staphylococcus aureus KCTC 1916, and can be used as a potent antibacterial agent against it.

• 대한수의학회지
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• 제41권2호
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• pp.173-176
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• 2001

개 피부병 유래 Microsporum canis 57주와 표준균주 1주 등 58주를 broth microdilution test로 계통이 다른 7가지 항진균제에 대한 감수성을 조사하고 고찰하였다. M canis의 항진균주에 대한 MIC(기하평균) 측정에서 terbinafine은 $MIC{\leq}0.004{\sim}0.031(0.007){\mu}g/ml$, itraconazole은 $MIC{\leq}0.004{\sim}0.125(0.029){\mu}g/ml$, ketoconazole은 MIC $0.015{\sim}0.5(0.097){\mu}g/ml$, tolnaftage은 MIC $0.031{\sim}1(0.155){\mu}g/ml$순으로 감수성이 높았고, griseofulvine은 MIC $0.063{\sim}2(0.285){\mu}g/ml$, amphotericin B는 MIC $0.125{\sim}2(0.540){\mu}g/ml$, flucytosine(5-Fe)은 MIC $4{\sim}64(26.430){\mu}g/ml$으로 감수성이 낮았다.

• Molecules and Cells
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• 제37권6호
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• pp.480-486
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• 2014

Growth restriction by antibiotics is a common feature that pathogenic bacteria must overcome for survival. The struggle of bacteria to escape from growth restriction eventually results in development of antibiotic-resistance through the expression of a set of genes. Here we found that some physiologically important transcriptional regulators of Pseudomonas aeruginosa including QscR, a quorum sensing (QS) receptor, SoxR, a superoxide sensor-regulator, and AntR, a regulator of anthranilate-related secondary metabolism, are activated by various growth-restricted conditions. We generated the growth-restricted conditions by various methods, such as overexpression of PA2537 and treatment with antibiotics or disinfectants. The overexpression of PA2537, encoding an acyltransferase homologue, tightly restricted the growth of P. aeruginosa and significantly activated QscR during the growth restriction. Similarly, treatments with gentamycin, tetracycline, and ethanol also activated QscR near their minimal inhibitory concentrations (MICs). Some non-QS regulators, such as AntR and SoxR, were also activated near the MICs in the same conditions. However, LasR and PqsR, other QS receptors of P. aeruginosa, were not activated, suggesting that only a specific set of transcriptional regulators is activated by growth restriction. Since paraquat, a superoxide generator, significantly activated QscR and AntR, we suggest that the oxidative stress generated by growth restriction may be partly involved in this phenomenon.

• 한국미생물·생명공학회지
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• 제38권3호
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• pp.289-294
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• 2010

단삼(Salvia miltiorrhiza Bunge)은 우리나라에서 전통적으로 부종, 관절염, 간염 등과 같은 염증질환의 치료에 사용해왔다. 본 연구에서는 단삼 메탄올 추출물을 이용하여 구강미생물에 대한 항균활성을 확인하였다. 그 결과 메탄올추출물은 실험 되어진 모든 구강미생물에서 강한 항균활성을 나타내었다(MICs, 8 to $64\;{\mu}g/mL$; MBCs, 16 to $64\;{\mu}g/mL$). 추가적으로 ampicillin이나 gentamicin과의 병용투여서 최소억제농도(MIC)와 최소살균농도(MBC)가 감소하는 병용효과를 나타내었다. 더불어 메탄올추출물 단독 사용시 보다 항생제와 병용투여시 빠른 사멸효과를 보였다. 결론적으로 단삼 메탄올추출물이 치아우식을 유발하는 많은 세균들에 대한 항균효과가 뛰어남을 확인 할 수 있었으며, 안정성이 높은 천연 치아우식예방제로서의 개발가능성을 확인하였다.

• 전기학회논문지
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• 제61권7호
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• pp.1069-1076
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• 2012

We use largely two methods, how to control the noise of the KTX, they are the passive noise control method and the active noise control method. The passive noise control has been used in a variety of ways since the KTX opening day, but lately it has shown the technical limitations by being dropped sharply. So, it is getting important to conduct the research about ANC that is able to reduce the ambient noise when the environmental-factor changes and be installed easily. To reduce a three-dimensional closed-space sound field like a car of a high-speed rail is hard to do using single channel ANC control system. Therefore we have to model the paths of the noise exactly for reducing the noise. And the control speakers and the error mics should be designed for optimal position. In this paper, we designed the transfer functions for modeling the noise paths under the influence of the distance between control speakers & error mics and primary noise speaker in TEST-BED where there is modeled as actual interior of KTX. We have made the modeling and the simulations of interior environment of KTX car by using three frequency bands of 120Hz, 280Hz, 360Hz. After the modeling, we compared the performance of active noise control and also we analyzed what to affect with difference in distance. After comparing of the performance using Pure Tone 120Hz, 280Hz, 360Hz at each modeling and then we simulated ANC for KTX's interior noise which we measured really and analyzed.

• 한방안이비인후피부과학회지
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• 제16권1호
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• pp.94-99
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• 2003

Objectives : Gram-positive bacteria have became increasing resistant to antibacterial agents, and hence multi-drug-resistant bacterial pathogens are now a major problem in clinical medicine. There is, therefore, a need for new antibacterial agents. In the course of our screening program for potent antibacterial agent from medicinal plants, the extract of Salvia miltiorrhiza (S. miltiorrhiza) showed antibacterial activity against methcillin resistant Staphylococcus aureus (MRSA) and antibiotic-resistant S. aureus. Methods : S. miltiorrhiza was extracted with 80$\%$ EtOH. The extract was suspended in H2O and fractionated successively with hexane chloroform, ethyl acetate, and n-buthanol. The chloroform fraction, which showed the highest antibacterial activity(MICs, 78㎍/ml) against MRSA, was chromatographed on a silica gel column and recycling prep-LC to give the pure antibacterial component. Results and Conclusions : The second fraction among the chloroform soluble portion of an aqueous EtOH extract of S. miltiorrhiza root showed outstanding antibacterial activity against MRSA and antibiotic-resistant S. aureus compared to the other fraction. An active compound was isolated from the second fraction using silica gel column chromatoraphy and recycling prep-LC. Based on these data together with the IH-, 13C-NMR, mass and mp, the active compounds were identified tanshinone Ⅰ, dehydrotanshinone Ⅰ and cryptotanshinone. Among tanshinones, cryptotanshinone and dihydrotanshinone Ⅰ MICs against MRSA and antibiotics-resistant S. aureus were 12.5, 12.5 and 6.3㎍/ml, respectively.

• Fisheries and Aquatic Sciences
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• 제24권10호
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• pp.319-329
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• 2021

In the search for antibiotic alternatives from safe and effective medicinal plants against fish pathogenic bacteria, we found that a combined extract (CE) of 1:1 (w/w) ratio of Angelica gigas Nakai roots and aerial parts of Artemisia iwayomogi Kitamura showed antibacterial activity against the fish pathogenic bacteria. By antibacterial activity-guided fractionations and isolations, five compounds were isolated and identified as decursinol angelate (1), decursin (2), xanthotoxin (3), demethylsuberosin (4), and 2,4-dihydroxy-6-methoxyacetophenone (5) through spectroscopic analyses, such as nuclear magnetic resonance (NMR) and mass spectrometry (MS). Among the compounds, 1 and 2 showed the highest antibacterial activities against Streptococcus iniae and Vibrio anguillarum, showing minimum inhibitory concentrations (MICs) of 62.5-250 ㎍/mL. Compounds 3, 4, and 5 were also found to be active, with MICs of 31.25-1,000 ㎍/mL for those strains. Furthermore, active compounds, 1 and 2 in CE were simultaneously quantified using high-performance liquid chromatography-tandem MS (HPLC-MS/MS). The average contents of 1 and 2 in CE was 3.68% and 6.14%, respectively. The established method showed reliable linearity (r² > 0.99), good precision, accuracy, and specificity with intra- and inter-day variations of < 2 % and recoveries of 90.13%-108.57%. These results may be helpful for establishing the chemical profile of CE for its commercialization as an antibiotic alternative in aquaculture.