• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2001.09a
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• pp.103-106
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• 2001

A remediation technology consisting of biodegradation and a modified Fenton reaction was developed to degrade mixtures of polycyclic aromatic hydrocarbons (PAHs) at a former manufactured gas plant (MGP) site. The original Fenton reaction (i.e., $H_2O$$_2$ + Fe$^{2+}$) was modified to be biocompatible by using ferric ions and chelating agents such as catechol and gallic acid. The modified reaction was effective in degrading PAHs at near neutral pH and thus was compatible with biodegradation. By the combined treatment of the modified Fenton reaction and biodegradation, more than 98% of 2- or 3-ring hydrocarbons and between 70 and 85% of 4- or 5-ring compounds were degraded in the MGP soil, while maintaining its pH about 6.6.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.29 no.6 s.237
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• pp.787-794
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• 2005

Nowadays chronic degenerative diseases such as arthritis are increasing rapidly, even though acute infectious diseases are decreasing due to the advance of modern medicine. Although many of remedies are developed for arthritis healing, there is no precise medical prescription, and pathogenesis is not examined exactly. In this study, we confirmed gene expression of BMP 1A, BMP 2B, osteonectin and MGP which are genes related with bone formation in osteoblast by using ultrasonic stimulation. Through this study, we also evaluated the fact that ultrasound could be applied to arthritis healing by making activated osteoblast induce remodeling of cartilage. As a result of this research, BMP 2B and MGP have higher rates of expression in specific ultrasound mode. In conclusion, it is expected that ultrasound could be used efficiently for healing arthritis if we use ultrasounds according to symptoms of arthritis on reasonable terms.

• Biotechnology and Bioprocess Engineering:BBE
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• v.6 no.2
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• pp.117-127
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• 2001

The high-level expression of a human single chain urokinase-type plasminogen activator (scu-PA) was achieved by employing a methotrexate (MTX)-dependent gene amplification system in Chinese hamster ovary (CHO) cells. By cotransfecting and coamplifying a scu-PA expression plasmid and dihydrofolate reductase (DHFR) minigene, several scu-PA expressing CHO cell lines were selected and gene-amplified. These recombinant cell lines, NGpUKs, secreted a completely processed scu-PA of 54 kD and up to 60mg/L was accumulated in the culture medium when they were adapted to an optimal MTX concentration. Over 95% of the scu-PA expressed was secreted in the culture medium and identified having the proper function of a plasminogen activator when activated by plasmin. Based on a genomic Southern analysis, a representative subclone, MGpUK-5, exhibited MTX-dependent scu-PA gene amplification, plus the initial single-copy gene of scu-PA eventually turned into about 150 copies of the amplified gene of scu-PA after gradual adaptation to 2.0$\mu$M of MTX. Meanwhile, the transcripts kof the scu-PA gene increased, although -early saturation of transcription was identified at 0.1$\mu$M of MTX. The scu-PA production by the MGpUK-5 subclone also increased relative to the gene amplification and increased transcripts, however, the relationship was not linearly proportional. Accordingly, since the MGpUK cell lines expressed elevated levels of enzymatically active scu-PA, these cell lines could be applied to the largescale production of scu-PA.

• Journal of Periodontal and Implant Science
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• v.32 no.3
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• pp.445-456
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• 2002

The purpose of this study were to determine that dexamethasone(Dex) induces differentiation of periodontal ligament(PDL) cells to osteoblastic cells and to investigate expression of matrix Gla protein(MGP), which is one of bone matrix protein. The isolated human PDL cells and gingival fibroblasts were prepared and cultured. The fourth or sixth sub-passage cells were used in this experiments. control group, ascorbic acid and ${\beta}$-glycerophosphate treated group, ascorbic acid, ${\beta}$-glycerophosphate and l00nM Dex treated group, ascorbic acid, ${\beta}$-glycerophosphate, and 5 ${\mu}M$ Dex treated group were made for study. The results were as follows: Cellular morphological change of PDL cells according to time was investigated. At first, the cells exhibited confluent monolayer of spindle or polygonal appearance. The multilayer of cells were seen after 7 days of treatment. After 14 days, the cells lost polarity and were densely packed. The mineralized nodule formation was seen at 21 days in the only Dex treated PDL cell groups. In the gingival fibroblast groups and no Dex treated PDL cell groups, the mineralized nodule was not seen. The mineralized nodule formation of 5 ${\mu}M$ Dex treated group was higher than 100 nM Dex treated group. Alkaline phosphatase(ALP) activity was higher in the Dex treated PDL cell groups of 14 and 21 days than 0 and 7 days. MGP was expressed in the control and all experimental groups and the expression was constant at 0,7,14,21 day. The above results confirm that Dex is affected to differentiation of the PDL cells to osteoblastic or cementoblastic cells and has dose-dependent effect for mineralization. And, MGP is expressed in the PDL cells and is not affected to mineralization of PDL cells.

• Journal of Marine Life Science
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• v.4 no.1
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• pp.38-43
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• 2019

The deformity occurring at the early developmental stage of red spotted grouper (Epinephelus akaara) causes detrimental effects on the process of juvenile production. In this study, we have compared the expressions of several key genes (insulin like growth factor 1: IGF-1, bone morphogenic protein 4: BMP4, peroxisome proliferator-activated receptors γ: PPARγ, matrix Gla protein: MGP) for morphogenesis between normal and 2 types (cephalic and jaw) of deformed juvenile fish. Expression of these genes were investigated in the brain, liver and muscle of each group of fish (n=20) by real-time PCR. Expression of IGF-1 and BMP4 mRNA in the brain and liver showed significant difference between normal and deformed fish (p<0.05). However, no difference was observed in the expression of PPARγ and MGP mRNA between normal and deformed fish in any tissues. It seems certain that IGF-1 and BMP4 are associated with the state of deformity in juvenile red spotted grouper.

• Proceedings of the Zoological Society Korea Conference
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• 2000.10a
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• pp.130.2-131
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• 2000

No Abstract, See Full Text

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.37 no.3
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• pp.181-189
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• 2001

This paper describes the positioning accuracy of GPS moduls (KGP9800C, KiRyung) which need to record the operating position of fishing boat as realtime processing in order to develope auto recording system of fishing boat's operating information, and then it's positioning accuracy for DGPS receiver (MGP-100D, Shin-A) and DGPS beacon (GP-36, Furuno) were analyzed. Futhermore, it was examined the positioning error of the GPS module with processing algorithm to minimize the effect of the signal fluctuations. The results indicate that the positioning error is able to decrease 5m as 2drms, and such improvement of positioning performance for GPS module (KGP9800C) can be provide the basic design data in the development of the auto recording system of fishing boat's operating information.

• Journal of Pharmacopuncture
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• v.17 no.3
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• pp.62-69
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• 2014

Objectives: The purpose of this study is to report a case series of advanced cancer patients whose cancer pain was relieved by using autonomic nerve pharmacopuncture (ANP) treatment. ANP is a subcutaneous injection therapy of mountain ginseng pharmacopuncture (MGP) along the acupoints on the spine (Hua-Tuo-Jia-Ji-Xue; 0.5 cun lateral to the lower border of the spinous processes of vertebrae) to enhance the immune system and to balance autonomic nerve function. Methods: Patients with three different types of cancer (gastric cancer, lung cancer, colon cancer with distant metastases) with cancer pain were treated with ANP. 1 mL of MGP was injected into the bilateral Hua-Tuo-Jia-Ji-Xue on the T1-L5 sites (total 12-20 mL injection) of each patient's dorsum by using the principle of symptom differentiation. During ANP treatment, the visual analogue scale (VAS) for pain was used to assess their levels of cancer pain; also, the dosage and the frequency of analgesic use were measured. Results: The cancer pain levels of all three patients improved with treatment using ANP. The VAS scores of the three patients decreased as the treatment progressed. The dosage and the frequency of analgesics also gradually decreased during the treatment period. Significantly, no related adverse events were found. Conclusion: ANP has shown benefit in controlling cancer pain for the three different types of cancer investigated in this study and in reducing the dosage and the frequency of analgesics. ANP is expected to be beneficial for reducing cancer pain and, thus, to be a promising new treatment for cancer pain.

• The Korean Journal of Ecology
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• v.23 no.2
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• pp.107-112
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• 2000

Comparison of oribatid mite community structures among Namsan, Kwangreung, and Mt, Jumbong, which receive different levels of environmental stress from severe to almost none, was made in coniferous and deciduous forests, respectively. The number of species of oribatid mites was significantly lower in Namsan and Mt. Jumbong than in Kwangreung in the coniferous forest (p<0.05). In the deciduous forests, the number of species of oribatid mites was significantly lower in Namsan than in Kwangreung and Mt. Jumbong. Dominant species in 3 regions were remarkably different. Similarity of the oribatid community between Namsan and Kwangreung was much higher (ca. 2 times) than similarities between Namsan and Mt. Jumbong. and Kwangreung and Mt Jumbong. Diversity index (H$^{\prime}$) value of oribatid communities in deciduous forests in Namsan, Kwangreung and Mt. Jumbong was 2.74, 2.78, and 2.87, respectively. Diversity (H$^{\prime}$) value of oribatid communities in coniferous forests in Namsan, Kwangreung and Mt. Jumbong was 2.83, 2.62, and 2.38, respectively. Namsan and Kwangreung were characterized as O-type in both coniferous and deciduous forests On the contrary, Mt. Jumbong was characterized as MG-type in MGP-I analysis.

• The Korean Journal of Soil Zoology
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• v.3 no.2
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• pp.72-77
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• 1998

This study was carried out to investigate the composition of soil microarthropods in pasture at the Unbong Area, to fulfil this purpose, samples were taken from National livestock research institute Namwon branch from 24. June, 1998 to 18. Setember. The results of this study were as follow. Collembola was hight in cattle and goat pasture, and Acarina was hight in wild and gathering pasture. Oribated mites were also hight in wild and gathering pasture more than cattle and goat pasture. Oribatid mites as a vectors of cestodes were identified 7 species of Carabodes peniculatus, Carabodes sp., Scheloribates laevigatus, Scheloribates latipes, Scheloribates rigidisetosus, Galumna cuneata and Galumna sp. The diversity indices showed hight in wild and gathering pasture. According to the MGP analysis I was type GP, and analysis II was type P.