• Animal Bioscience
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• 제37권5호
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• pp.875-882
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• 2024

Objective: An experiment was conducted to study the effect of supplementing DL methionine (DL Met) at graded concentrations on performance, carcass variables, immune responses and antioxidant variables in broiler chicken fed folic acid (FA) fortified (4 mg/kg) low-methionine diet. Methods: A basal diet (BD) without supplemental DL Met, but with higher level (4 mg/kg) of FA and a control diet (CD) with the recommended concentration of methionine (Met) were prepared. The BD was supplemented with DL Met at graded concentrations (0%, 10%, 20%, 30%, 40%, and 50% supplemental DL Met of CD). Each diet was fed ad libitum to 10 replicates of 5 broiler male chicks in each from 1 to 42 d of age. Results: Body weight gain (BWG) reduced, and feed conversion ratio (FCR) increased in broilers fed low-Met BD. At 30% and 20% inclusion of DL met, the BWG and FCR, respectively were similar to those fed the CD. Similarly, supplementation of 10% DL Met to the BD significantly increased ready to cook meat yield and breast meat weight, which were similar to those of the CD fed broilers. Lipid peroxidation reduced, the activity of antioxidant enzymes (GSHPx and GSHRx) in serum increased and lymphocyte proliferation increased with increased supplemental DL Met level in the BD. The concentrations of total protein and albumin in serum increased with DL Met supplementation to the BD. Conclusion: Based on the data, it can be concluded that supplemental Met can be reduced to less than 50% in broiler chicken diets (4.40, 3.94, and 3.39 g/kg, respectively in pre-starter, starter and finisher phases) containing 4 mg/kg FA.

• Plant Biotechnology Reports
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• 제3권2호
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• pp.139-145
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• 2009

DNA methylation is known to play an important role in the regulation of gene expression in eukaryotes. In this study, we isolated NtMET1 from Nicotiana tabacum cv. Havana (SR1) and obtain transgenic plants that reduced MET1 expression level with the double-strand RNA (dsRNA) MET1 gene. Transgenic tobacco plants showed dwarf and abnormal flower development when compared with the wild type. Using methylation-sensitive amplified polymorphism (MSAP) analysis, the patterns of cytosine methylation in transformed plants and the wild type were compared. MseI/HpaII selection primers showed an interesting polymorphism, and 153 DNA bands of interest were detected. Among these, 30 selective fragments were sequenced and analyzed with a BLAST search by successful MSAP modifications. The homology search showed that the transposons and tandem repeated sequences were related to the phenotypes. These results suggested that the decreased degree of methylation by dsRNA strategy caused abnormal growth and development in N. tabacum.

• 한국작물학회지
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• 제26권3호
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• pp.251-256
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• 1981

수입에 의 한 수요충족이 절대불가능한 양질다수성 싸이레지용 옥수수의 개발은 우리나라에 매우 필요한 사항이다. 양질 다수성인 싸이레지용 옥수수의 개발을 위한 육종적 재료를 확보코저 재래종 옥수수의 수집종 가운데서 선발된 다약다수성인 계통을 가지고 재배환경이 다양한 조건하에서 싸이레지용으로서의 가부시험을 한 결과는 다음과 같이 요약된다. 1. 싸이레지용 주요특성의 하나인 단위면적당 총건물중 생산양에 있어서 파종기나 재배밀도에 관계없이 수원 19호 교잡종보다 다약다종성인 MET 계통이 평균 10% 더 높았다. 2. 식물체의 부입별 특성을 비교하면 MET 계통은 건엽중이 수원 19호보다 월등히 높아 MET 계통의 건물중 증가에 기여하였다. 3. MET 계통의 건엽중이 수원 19호보다 높았던 것은 MET계통의 분벽에 의한 엽수의 증가 때문이었으며, 4. 기타 곡실수량에 관련된 이삭의 무게라든가 입중, 이확속중 등은 수원 19호가 높았다. 다만 포엽중만은 MET 계통이 다수성으로 인해 더 높았다. 5. TDN의 증감과 관계가 있는 100a당 배의 생산은 소입성인 MET 계통이 수원 19호보다 10$\sim$15% 더 높았다. 6. MET 계통이 다약다수성이고 수원 19호가 무약일수성인데도 불구하고 분산분석결과 품종$\times$재배환경(파종기 또는 재식밀도)라는 유의적인 상호작용이 없이 다 같이 밀식함으로써 총건물중이 증가하였다. 7. 싸이레지로 만들었을 때 MET 계통과 수원 19호의 일반조성분 TDN 등을 비교한 결과 일반 조성분에 있어서는 두 품종간에 큰 차이가 없었으나 TDN에 있어서는 MET 계통이 더 높았고 DCP에 있어서는 수원 19호가 더 높았다. 8. 아미노산의 분석결과 두 품종간에 큰 차이는 없었다.

• The Korean Journal of Physiology and Pharmacology
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• 제26권2호
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• pp.95-111
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• 2022

Chronic obstructive pulmonary disease (COPD) is an important healthcare problem worldwide. Often, glucocorticoid (GC) resistance develops during COPD treatment. As a classic hypoglycemic drug, metformin (MET) can be used as a treatment strategy for COPD due to its anti-inflammatory and antioxidant effects, but its specific mechanism of action is not known. We aimed to clarify the role of MET on COPD and cigarette smoke extract (CSE)-induced GC resistance. Through establishment of a COPD model in rats, we found that MET could improve lung function, reduce pathological injury, as well as reduce the level of inflammation and oxidative stress in COPD, and upregulate expression of nuclear factor E2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), multidrug resistance protein 1 (MRP1), and histone deacetylase 2 (HDAC2). By establishing a model of GC resistance in human bronchial epithelial cells stimulated by CSE, we found that MET reduced secretion of interleukin-8, and could upregulate expression of Nrf2, HO-1, MRP1, and HDAC2. MET could also increase the inhibition of MRP1 efflux by MK571 significantly, and increase expression of HDAC2 mRNA and protein. In conclusion, MET may upregulate MRP1 expression by activating the Nrf2/HO-1 signaling pathway, and then regulate expression of HDAC2 protein to reduce GC resistance.

• 열처리공학회지
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• 제17권1호
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• pp.50-54
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• 2004

• Nutrition Research and Practice
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• 제15권1호
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• pp.80-94
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• 2021

BACKGROUND/OBJECTIVES: Recent studies have reported chocolate consumption has beneficial effects on blood pressure, obesity, and dyslipidemia, which are components of metabolic syndrome (MetS). However, evidence regarding these relationships in Asia is limited. The aim of this study was to investigate relations between chocolate consumption and MetS in Korea. SUBJECTS/METHODS: Study subjects were 6,982 adults aged 19-64 years free of major chronic diseases that participated in the 2014-2016 Korea National Health and Nutrition Examination Survey. Information on chocolate consumption was obtained using food frequency questionnaires. For primary analysis, subjects were divided into two groups: non-consumer (n = 3,741, 53.6%); consumer (n = 3,241, 46.4%). In order to investigate dose-response effects on MetS, chocolate consumers were divided into quartiles based on amounts consumed: Q1 (n = 789, 24.3%); Q2 (n = 837, 25.8%); Q3 (n = 863, 26.6%); Q4 (n = 752, 23.2%) in second analysis. RESULTS: Chocolate consumers; 1) were more physically active(P < 0.0001); 2) less likely to smoke(P < 0.0001); 3) paid more attention to food nutrition labels (P < 0.0001), and 4) had healthier dietary patterns, that is, they consumed vegetables, fruits, and dairy products more frequently(P < 0.0001) than non-consumers. Furthermore, chocolate consumers had a 30% lower risk of MetS (odds ratio, 0.70; 95% confidence interval, 0.58-0.86; P = 0.0005). However, dose-response effects between chocolate intake and MetS was not found by secondary analysis after excluding non-consumers. CONCLUSIONS: In conclusion, chocolate consumers had healthier lifestyles and dietary patterns and a lower prevalence of MetS than non-consumers, as determined by our primary analysis. Although dose-response effects were not found to be significant among chocolate consumers during secondary analysis, the patterns were partially similar. This cross-sectional finding shows chocolate consumption does not increase the risk of MetS. The prospective studies and intervention trials in Korea are needed to confirm this cross-sectional finding.

• 정보관리학회지
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• 제38권4호
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• pp.153-171
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• 2021

이 연구는 데이터세트를 관리, 보존, 서비스하기 위해 DDI 메타데이터를 기반으로 METS를 활용하는 방안을 제시하였다. DDI는 통계 데이터 처리를 위한 표준으로 현재 DDI Codebook(DDI-C)과 DDI Lifecycle(DDI-L) 두 가지 버전이 존재한다. 본 연구에서는 DDI-C의 주요 요소를 주로 하였다. 이를 위해 우선 METS와 DDI-C의 구조와 요소를 분석하였다. 그리고 METS와 DDI-C의 주요 요소들에 대한 매핑작업을 수행하였다. 여기서 기준은 최종적으로 이를 표현할 형식인 METS로 삼았다. METS와 DDI-C가 완벽하게 1:1의 매핑을 보이지 않기 때문에 기준인 METS의 각 요소들에 가장 적합하게 부합하는 DDI-C 요소를 선택하였다. 그 결과 DDI-C 메타데이터요소를 활용한 새로운 데이터세트 관리전송 표준 METS를 설계하여 제시하였다.

• 한국미생물·생명공학회지
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• 제42권1호
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• pp.11-17
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• 2014

Selenium(Se)은 사람에게 필수성분이지만 독성이 강한 물질이다. 따라서 생체에서 Se(셀레늄)의 농도조절은 정확하고 효율적으로 이루어져야 한다. 사람은 Se을 음식의 형태로 섭취해야 하고 주로 seleno-L-methionine (L-SeMet)의 형태로 섭취한다. 섭취된 L-SeMet은 methionine 대사와 동일한 효소를 이용하여 Se-adenosyl-L-SeMet으로 대사된다고 알려져 있었다. 그러나 최근 쥐의 간 추출물의 실험에서, L-SeMet이 cystathionine ${\gamma}$-lyase (mouse CGL)의 작용으로 methylselenol ($CH_3SeH$)로 직접적으로 대사된다는 보고가 있었다. CGL은 원래 cystathionine을 L-cysteine과 ${\alpha}$-ketobutyrate, 그리고 $NH_3$로 분해하는 효소로 알려져 있었다. 따라서 본 연구에서는 쥐의 간 추출물 대신에 인간의 CGL을 분리 정제하여 L-SeMet에서 methylselenol의 형성을 확인하고자 하였다. Methylselenol의 표준시료는 dimethyldiselenide를 sodium tetrahydroborate로 환원시켜 준비하였다. 그리고 L-SeMet을 기질로 사용한 효소 반응액 중에서 가스상의 생성물은 GC/MS 스펙트럼으로 분석하였다. 분석 결과 methylselenol의 유도체인 dinitrophenyl selenoether와 일치하였다. 또한 인간 CGL이 L-SeMet에서 methylselenol을 형성하는 반응의 kinetic parameter를 mouse CGL과 비교 분석하였다. 결과적으로 human CGL은 섭취된 L-SeMet의 대사를 책임지고 있는 중요한 효소이다.

• 한국균학회지
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• 제21권4호
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• pp.279-284
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• 1993

담자균류의 영지버섯으로부터 homocysteine methyltransferase를 code하는 metE 유전자를 methionine 요구성 균주인 대장균에 complementation시켜 cloning하였다. 그 결과 삽입된 DNA의 크기는 약 1.54 kb 이었고, 5개의 제한효소 부위가 존재하였다. 이 clone체의 제한지도를 작성하였고, southern blot 분석으로 metE 유전자는 영지버섯의 genome으로부터 유래하였으며, 단일 복제수로 존재함을 확인하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권5호
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• pp.714-718
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• 2003

This study was conducted to determine the effect of DL-methionine (Met) and DL-methionine hydroxy analogue (MHA) supplementation on the growth performance, contents of serum amino acids and activities of digestive protease in broiler chicks from 0 to 3 weeks old . There were three treatments in this study: (1) control (basal diet), (2) 0.24% Met supplementation and (3) 0.368% MHA supplementation. The results showed that Met and MHA supplementation did not significantly (p>0.05) improve feed efficiency and body weight gain for broilers from 0-1, 0-2 and 0-3 weeks of age. The serum levels of homocystine, methionine and taurine were significantly (p<0.05) higher with supplementation of Met or MHA than with control. The pepsin activity of proventriculus was increased with (p<0.05) Met supplementation at 21 days of age and with MHA supplementation at 7 and 14 days of age. The trypsin activity was also increased (p<0.05) with MHA supplementation at 7 days of age. The chymotrypsin activity in pancreas and the dipeptidase activity in small intestinal mucosa and content were not affected (p>0.05) by Met or MHA supplementation.