• BMB Reports
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• 제45권2호
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• pp.126-131
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• 2012

We have previously identified a DNA silent region located downstream of the 3'-end of the ${\beta}^{major}$ globin gene (designated B1-559) that contains a B1 retrotransposon, consensus binding sites for erythroid specific transcription factors and shares the capacity to act as promoter in hematopoietic cells interacting with ${\beta}$-globin gene LCR sequences in vitro. In this study, we have cloned four new non-polyA RNA transcripts being detected upon blockade of murine erythroleukemia (MEL) cell differentiation to erythroid maturation by methylation inhibitors and demonstrated that two of them share high structural homology with sequences of B1 element found within the B1-559 region. Although it is not clear yet whether and how these RNAs interfere with induction of erythroid maturation, these data provide evidence for the first time showing that methylation inhibitors can activate silent repetitive DNA sequences in MEL cells and may have implications in cancer chemotherapy using demethylating drugs as antineoplastic agents.

• Parasites, Hosts and Diseases
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• 제37권4호
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• pp.289-292
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• 1999

BALB/c mice infected with a high virulent strain of Trypanosoma brucei gambiense IL3707 were treated intraperitoneally (ip) with either Melarsoprol （Mel-B) or PSG(+) buffer as controls. The mice were subsequently monitored regularly for parasites by direct microscopic examination of their tail blood or buffy coat and by polymerase chain reaction (PCR). Mel-B was found to be an effective drug for treatment against T.b. gambiense because at the end of the first treatment schedule, all treated mice were negative for parasites even by PCR, while all the control animals were positive. Three of the five Mel-B treated mice, while parasitologically negative, were PCR positive between 53 and 80 days post infection (DPI), indicating that they still harbored an infection. All treated mice were subsequently negative for parasites even by PCR at 88 DPI. A combination of conventional microscopic examination and PCR offers a good prediction of cure following treatment of trypanosomosis.

• 한국산업정보학회논문지
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• 제6권4호
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• pp.81-88
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• 2001

본 논문에서는 한국어 숫자음 인식을 위해 다층 퍼셉트론을 이용한 인식시스템을 구현하였으며 음성인식 분야에서 일반적으로 널리 사용되는 여러 종류의 특징을 인식시스템의 입력으로 적용하여 각각의 인식 성능 및 특성을 알아보았다. 이를 위해 Mel-scale-Filterbank 계수, MFCC, LPCC, 그리고 PLP 계수를 입력 특징으로 사용하였다. 본 논문에서는 제한된 환경이 아닌 여러 종류의 잡음이 존재하는 일반적인 환경에서도 견실한 성능을 보일 수 있는 인식시스템을 구현하기 위해 잡음이 거의 포함되지 않은 음성 데이터뿐만 아니라 잡음이 첨가된 음성 데이터에 대해 인식 실험을 각각 수행하였다. 실험에서는 20개의 한국어 숫자음에 대한 인식 실험을 수행하였으며 그 결과로부터 Mel-scale Filterbank 계수가 잡음의 첨가 유무에 관계없이 화자 종속 및 화자 독립적인 음성 데이터에 대해 가장 견실한 인식 성능을 보임을 확인할 수 있었다.

• 한방안이비인후피부과학회지
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• 제16권3호
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• pp.145-163
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• 2003

Objectives : This study was performed to investigate the depigmentation effects of Aloe, Camellia sinensis and Mel. Methods : Inhibition of tyrosinase activity, melanin production & melanoma cell viability in cultured B16 melanoma cells, UV screen and cytoprotective effects on PC12 cells injured by hydrogen peroxide were measured. Results : Aloe has some inhibitory effects on tyrosinase activity, on the other hand Camellia sinensis and Mel do not have. They did not show any inhibitory effects on melanin production in melanoma cells and cytoprotective effects on PC12 cells injured by hydrogen peroxide. Aloe and Camellia sinensis have some inhibitory effects on UV screen. Conclusions : This study shows that Aloe and Camellia sinensis which were generally used for external application have some depigmentation effects. Following this, We should use them for whitening agents and the depigmentation effects of the other natural subjects which were generally used for external application should be examined.

• 한국수의병리학회지
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• 제2권1호
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• pp.25-30
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• 1998

An in vivo model for human melanoma was established with the growth and metastasis of SK-MEL-2 cells. The tumor was introduced into C.B-17 SCID(severe combined immunodeficiency) mice intraperiotneally subcutaeously and intravenous inoculations. Tumors developed in 100% of mice inoculated subcutaneously and intraeritoneally both at site of inoculation and as metastatic tumor in the liver lungs and diaphragm. With intravenous inoculation 50% of mice showed metastasis in the spleen. Additionally metastatic foci that were not detected either by gross and/or standard histopathologic examination were demonstrated in the spleen and lungs by immunohistochemistry with HMB-45 monoclonal antibody. We conclude that the SCID mouse supports growth and metastasis of human malignant melanoma SK-MEL-2 cells.

• Environmental Analysis Health and Toxicology
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• 제21권4호
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• pp.365-373
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• 2006

Emission of methyl bromide (MeBr) from soil was implicated in stratospheric ozone depletion. To determine multi analysis of alternatives fumigants for MeBr, this paper describes the methods of analysis in water and soil. The MeBr, methyl iodide (Mel), propargyl bromide (PBr), cis 1,3-dichloropropene (cis 1,3-D), trans 1,3-dichloropropene (trans 1,3-D) and chloropicrin(CP) are separated on the base line on GC-ECD at three column of AT+DB+DB (90m) with temperature programing of $35^{\circ}C{\rightarrow}110^{\circ}C$ on GC-ECD. The relative retention time for MeBr, Mel, PBr, cis 1,3-D, trans 1,3-D and CP is 1.0, 1.4, 2.3, 3.2, 3.6 and 3.7, respertively. The detection limit for MeBr, Mel, PBr, cis 1,3-D, trans 1,3-D and CP is 469 pg, 5 pg, 21 pg, 79 pg, 101 PE and 5pg, respectively. Recovery of MeBr Mel, PBr, cis 1,3-D, trans 1,3-D and CP in water added 150 ppm fumigants were 81%, 96%, 95%, 97%, 98% and 99%, respectively. Recovery of MeBr, MeI, PBr, cis 1, 3-D, trans 1,3-D and CP in soil added 150ppm fumigants were 56%, 84%), 85%, 81%, 87% and 88%, respectively.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
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• pp.544-544
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• 2003

We investigated the inhibitory effect of whitening materials with growth factor or alone on melanomas derived from Human (B-16) and mouse (SK-MEL-31) using melanin content. Melanin content was determined by the absorbance value at 470nm per cells. we used the growth factors known as activators of Adenylate cyclase, Protein kinase C and tyrosine kinase pathway separately. In addition, we compared the action of UV-induced with non-biological growth factor with whitening materials in melanomas derived from Human and mouse. The results showed that the aspect of inhibitory effect of whitening materials on B16 and SK-MEL-31 was not different. And, the action of each growth factor involved in the differentiation and proliferation of melanoma on the inhibition of melanogenesis in B-16 and SK-MEL-31 using whitening agents showed no difference. Also, The action of UV -induced and non-biological growth factors didn't exhibit different pattern on the effect of whitening agent in B-16 and SK-MEL-31.

• 한국식품영양과학회지
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• 제24권1호
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• pp.160-168
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• 1995

Desmutagenicities against 2-amino-1-methyl-6-phenylimidazo[4, 5-b] pyridine(PhIP) and 2-amino-3, 8-dimethylimidazo[4, 5-f]quinoxaline(MelQx) of tea extracts (steamed green tea, roasted green tea, oolong tea and black tea) were investigated. All the fractions obtained from tea extracts showed strong desmutagenic activity against PhIP and MeIQx toward S. typhimurium TA 98 in the presence of the S-9 mix. The crude catechin fraction exhibited the strongest desmutagenic activity. Among these tea extracts, black tea especially exhibited the strongest desmutagenic activity and the activity was 70.9~91.0% against PhIP and 92.2~98.8% against MelQx at a concentration(0.5~1.0mg/plate) for drinking. The activity of authentic catechins of (-)-EGC, (-)-EGCg, (-)-ECg and (-)-EC were 79.5%, 60.2%, 46.1% and 43.5% against PhIP, and were 52.3%, 11.6%, 8.2% and 22.1% against MelQx by addition of 1.0mg/plate, respectively. The desmutagenic activity was supposedly due to the (-)-EGCg, (-)-EGC and (-)-EC in tea polyphenols, and the browning materials. The desmutagenicity was stronger when mutagens were preincubated with S-9 mix after reaciton with black tea extracts than when preincubated with them after reaction with S-9 mix. The desmutagenicity of tea extracts was rather expressed by reacting directly with mutagens than by deactivating the activated forms of mutagens.

• 한국컴퓨터정보학회:학술대회논문집
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• 한국컴퓨터정보학회 2023년도 제67차 동계학술대회논문집 31권1호
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• pp.43-46
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• 2023

호흡기 관련 전염병의 주된 증상인 기침은 공기 중에 감염된 병원균을 퍼트리며 비감염자가 해당 병원균에 노출된 경우 높은 확률로 해당 전염병에 감염될 위험이 있다. 또한 사람들이 많이 모이는 공공장소 및 실내 공간에서의 기침 탐지 및 조치는 전염병의 대규모 유행을 예방할 수 있는 효율적인 방법이다. 따라서 본 논문에서는 탐지해야 하는 기침 소리 및 일상생활 속 발생할 수 있는 기침과 유사한 배경 소리 들을 Mel-Spectrogram으로 변환한 후 시각화된 특징을 CNN 모델에 학습시켜 기침 탐지를 진행하며, 일반적으로 사용되는 사전 학습된 CNN 모델에 제안된 Attention 모듈의 적용이 기침 탐지 성능 향상에 도움이 됨을 입증하였다.

• Journal of Acupuncture Research
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• 제18권4호
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• pp.101-115
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• 2001

Objective : To characterize the antitumorigenic potential of Apamin, one of the major components of bee venom, its effects on cell proliferation and the mitogen-activated protein kinase (MAPK) signal transduction pathway were characterized using the human melanoma cell line SK-MEL-2. Methods & Results : Cell counting analysis for cell death demonstrated that consistent with a previous results, SK-MEL-2 cells treated with $0.5-2.0{\mu}g/ml$ of Apamin showed no recognizable cytotoxic effect whereas detectable induction of cell death was identified at concentrations over $5.0{\mu}g/ml$. [3H]thymidine incorporation assay for cell proliferation demonstrated that DNA replication of SK-MEL-2 cells is inhibited by Apamin in a dose- and time-dependent manner. To explore whether Apamin-induced growth suppression is associated with the MAPK signaling pathway, phosphorylation of Erk, a function mediator of MAPK growth-stimulating signal, was examined Western blot assay using a phospho-specific Erkl/2 antibody. A significant increase of Erkl/2 phosphorylation level was observed in Apamin-treated cells compared with untreated control cells. Qantitative RT-PCR analysis revealed that Apamin inhibit expression of MAPK downstream genes such as c-Jun, c-Fos, and cyclin D1 but not expression of MAPK pathway component genes including Ha-Ras, c-Raf-1, MEK1, and Erk. Conclusion : It is strongly suggested that the antitumorigenic activity of Apamin might result in part from its inhibitory effect on the MAPK signaling pathway in human melanoma cells SK-MEL-2.