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Stock identification of minor carp, Cirrhinus reba, Hamilton 1822 through landmark-based morphometric and meristic variations

  • Ethin, Rokhsana;Hossain, Md Shakhawate;Roy, Animesh;Rutegwa, Marcellin
    • Fisheries and Aquatic Sciences
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    • v.22 no.6
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    • pp.12.1-12.8
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    • 2019
  • Background: Wild fish populations stock is continuously diminishing in the Indo-Ganges river basin, and the population status of most fishes is unidentified. The identification of the population status and the conservation of commercially important and endemic wild fish populations in this region are crucial for the management. The aim of this paper was to identify the population status of Cirrhinus reba, a promising aquaculture but vulnerable species in the Indo-Ganges river basin in Bangladesh. Methods: C. reba samples were collected from four isolated populations of the Brahmaputra (n = 30), the Padma (33), the Karatoya (31), and the Jamuna Rivers (30) in Bangladesh, and the population status was evaluated using morphometric and landmark comparisons. Data were analyzed with the Kruskal-Wallis test, univariate analysis, discriminant function analysis, and the formation of a dendrogram. Results: Three meristic characters (Pectoral fin rays, caudal fin rays, scale in lateral lines), four morphometric characters (head length, pre-orbital length, post-orbital length, maximum body depth), and truss measurement (4-7) were significantly different among the stocks. The step-wise discriminant function analysis retained 15 variables from morphometric and landmark measurements that significantly differentiated the populations based on the constructed DFI and DFII. Discriminate function analysis also showed that 91.2% of the original groups were classified into their correct samples. The cluster analysis of Euclidean distances placed the Jamuna population in one cluster and the Brahmaputra, the Padma, and the Karatoya populations in the second one. Conclusion : Morphological differences among the stock were probably due to different ancestral origin. This is the first report about population status of C. reba in their natural habitat of the Indian subcontinent. Further genetic studies and the evaluation of environmental impact on C. reba populations in Bangladesh are suggested to support our findings.

Ultrafiltration of palm oil mill effluent: Effects of operational pressure and stirring speed on performance and membranes fouling

  • Yunos, Khairul Faezah Md;Mazlan, Nurul Ain;Naim, Mohd Nazli Mohd;Baharuddin, Azhari Samsu;Hassan, Abdul Rahman
    • Environmental Engineering Research
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    • v.24 no.2
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    • pp.263-270
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    • 2019
  • Palm oil mill effluent (POME) is the largest pollutant discharged into the rivers of Malaysia. Thus UF membrane study was conducted to investigate the effect of pressure and stirring speed on performance of POME treatment and fouling of membrane. Two types of membrane polyethersulfone (PES) and regenerated cellulose (RC) with molecular weight cut-off (MWCO) 5 and 10 kDa were used in this study. Results showed that, as pressure increased, fouling increased however permeate quality improved, the best pressure was 1.0 bar, where the fouling was not too high and produce good permeate quality. As stirring speed increased, fouling reduced and permeate quality improved, however, when stirring speed increased from 600 rpm to 800 rpm, there was no significant improvement on the permeate quality. Therefore, the best condition was at 1.0 bar and 600 rpm. PES membrane with MWCO 5 kDa showed the best permeate quality, even fouling slightly higher than RC membrane. The permeate quality obtained were analyzed in term of dissolved solid, turbidity, suspended solid, biological oxygen demand ($BOD_5$) and chemical oxygen demand (COD) were 538 mg/L, 1.02 NTU, < 25 mg/L, 27.7 mg/L and 62.8 mg/L, respectively with dominant type of fouling is cake resistance. Thus, it can be concluded water reuse standard was successfully achieved in terms of $BOD_5$ and suspended solid.

Two person Interaction Recognition Based on Effective Hybrid Learning

  • Ahmed, Minhaz Uddin;Kim, Yeong Hyeon;Kim, Jin Woo;Bashar, Md Rezaul;Rhee, Phill Kyu
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.13 no.2
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    • pp.751-770
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    • 2019
  • Action recognition is an essential task in computer vision due to the variety of prospective applications, such as security surveillance, machine learning, and human-computer interaction. The availability of more video data than ever before and the lofty performance of deep convolutional neural networks also make it essential for action recognition in video. Unfortunately, limited crafted video features and the scarcity of benchmark datasets make it challenging to address the multi-person action recognition task in video data. In this work, we propose a deep convolutional neural network-based Effective Hybrid Learning (EHL) framework for two-person interaction classification in video data. Our approach exploits a pre-trained network model (the VGG16 from the University of Oxford Visual Geometry Group) and extends the Faster R-CNN (region-based convolutional neural network a state-of-the-art detector for image classification). We broaden a semi-supervised learning method combined with an active learning method to improve overall performance. Numerous types of two-person interactions exist in the real world, which makes this a challenging task. In our experiment, we consider a limited number of actions, such as hugging, fighting, linking arms, talking, and kidnapping in two environment such simple and complex. We show that our trained model with an active semi-supervised learning architecture gradually improves the performance. In a simple environment using an Intelligent Technology Laboratory (ITLab) dataset from Inha University, performance increased to 95.6% accuracy, and in a complex environment, performance reached 81% accuracy. Our method reduces data-labeling time, compared to supervised learning methods, for the ITLab dataset. We also conduct extensive experiment on Human Action Recognition benchmarks such as UT-Interaction dataset, HMDB51 dataset and obtain better performance than state-of-the-art approaches.

Prognostic role of EGR1 in breast cancer: a systematic review

  • Saha, Subbroto Kumar;Islam, S.M. Riazul;Saha, Tripti;Nishat, Afsana;Biswas, Polash Kumar;Gil, Minchan;Nkenyereye, Lewis;El-Sappagh, Shaker;Islam, Md. Saiful;Cho, Ssang-Goo
    • BMB Reports
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    • v.54 no.10
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    • pp.497-504
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    • 2021
  • EGR1 (early growth response 1) is dysregulated in many cancers and exhibits both tumor suppressor and promoter activities, making it an appealing target for cancer therapy. Here, we used a systematic multi-omics analysis to review the expression of EGR1 and its role in regulating clinical outcomes in breast cancer (BC). EGR1 expression, its promoter methylation, and protein expression pattern were assessed using various publicly available tools. COSMIC-based somatic mutations and cBioPortal-based copy number alterations were analyzed, and the prognostic roles of EGR1 in BC were determined using Prognoscan and Kaplan-Meier Plotter. We also used bc-GenEx-Miner to investigate the EGR1 co-expression profile. EGR1 was more often downregulated in BC tissues than in normal breast tissue, and its knockdown was positively correlated with poor survival. Low EGR1 expression levels were also associated with increased risk of ER+, PR+, and HER2- BCs. High positive correlations were observed among EGR1, DUSP1, FOS, FOSB, CYR61, and JUN mRNA expression in BC tissue. This systematic review suggested that EGR1 expression may serve as a prognostic marker for BC patients and that clinicopathological parameters influence its prognostic utility. In addition to EGR1, DUSP1, FOS, FOSB, CYR61, and JUN can jointly be considered prognostic indicators for BC.

Development of a Simultaneous Analytical Method for Diquat, Paraquat and Chlormequat in Animal Products Using UPLC-MS/MS

  • Cho, Il Kyu;Rahman, Md. Musfiqur;Seol, Jae Ung;Noh, Hyun Ho;Jo, Hyeong-Wook;Moon, Joon-Kwan
    • Korean Journal of Environmental Agriculture
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    • v.39 no.4
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    • pp.368-374
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    • 2020
  • BACKGROUND: The residual analysis of polar pesticides has remained a challenge. It is even more difficult to simultaneously analyze multiple polar pesticides. Diquat, paraquat, and chlormequat are typical examples of highly polar pesticides. The existing methods for the analysis of diquat, paraquat and chlormequat are complex and time consuming. Therefore, a simple, quick and effective method was developed in the represent study for simultaneous analysis of diquat, paraquat and chlormequat in animal products, meat and fat using UPLC-MS/MS. METHODS AND RESULTS: Sample extraction was carried out using acidified acetonitrile and water and re- extracted with acidified acetonitrile and combine the extracts followed by centrifugation. The extract was then cleaned up with a HLB cartridge after reconstitution with acidic acetonitrile and water. The method was validated in quintuplicate at three different concentrations. The limits of detection (LOD) and quantification (LOQ) were 0.0015 and 0.005 mg/L, respectively. Matrix suppression effect was observed for all of the analytes. A seven point matrix matched calibration curve was constructed for each of the compound resulted excellent linearity with determination coefficients (R2) ≥ 0.991. Accuracy and precision of the method was calculated from the recovery and repeatability and ranged from 62.4 to 119.7% with relative standard deviation less than 18.8%. CONCLUSION: The recovery and repeatability of the developed method were in the acceptable range according to the Codex Alimentarius guideline. The developed method can be applied for the routine monitoring of diquat, paraquat, and chlormequat in animal products, meat and fat.

The Membrane-Bound Protein, MoAfo1, Is Involved in Sensing Diverse Signals from Different Surfaces in the Rice Blast Fungus

  • Sadat, Md Abu;Han, Joon-Hee;Kim, Seongbeom;Lee, Yong-Hwan;Kim, Kyoung Su;Choi, Jaehyuk
    • The Plant Pathology Journal
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    • v.37 no.2
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    • pp.87-98
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    • 2021
  • To establish an infection, fungal pathogens must recognize diverse signals from host surfaces. The rice blast fungus, Magnaporthe oryzae, is one of the best models studying host-pathogen interactions. This fungus recognizes physical or chemical signals from the host surfaces and initiates the development of an infection structure called appressorium. Here, we found that protein MoAfo1(appressorium formation, MGG_10422) was involved in sensing signal molecules such as cutin monomers and long chain primary alcohols required for appressorium formation. The knockout mutant (ΔMoafo1) formed a few abnormal appressoria on the onion and rice sheath surfaces. However, it produced normal appressoria on the surface of rice leaves. MoAfo1 localized to the membranes of the cytoplasm and vacuole-like organelles in conidia and appressoria. Additionally, the ΔMoafo1 mutant showed defects in appressorium morphology, appressorium penetration, invasive growth, and pathogenicity. These multiple defects might be partially due to failure to respond properly to oxidative stress. These findings broaden our understanding of the fungal mechanisms at play in the recognition of the host surface during rice blast infection.

Web-based University Classroom Attendance System Based on Deep Learning Face Recognition

  • Ismail, Nor Azman;Chai, Cheah Wen;Samma, Hussein;Salam, Md Sah;Hasan, Layla;Wahab, Nur Haliza Abdul;Mohamed, Farhan;Leng, Wong Yee;Rohani, Mohd Foad
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.16 no.2
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    • pp.503-523
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    • 2022
  • Nowadays, many attendance applications utilise biometric techniques such as the face, fingerprint, and iris recognition. Biometrics has become ubiquitous in many sectors. Due to the advancement of deep learning algorithms, the accuracy rate of biometric techniques has been improved tremendously. This paper proposes a web-based attendance system that adopts facial recognition using open-source deep learning pre-trained models. Face recognition procedural steps using web technology and database were explained. The methodology used the required pre-trained weight files embedded in the procedure of face recognition. The face recognition method includes two important processes: registration of face datasets and face matching. The extracted feature vectors were implemented and stored in an online database to create a more dynamic face recognition process. Finally, user testing was conducted, whereby users were asked to perform a series of biometric verification. The testing consists of facial scans from the front, right (30 - 45 degrees) and left (30 - 45 degrees). Reported face recognition results showed an accuracy of 92% with a precision of 100% and recall of 90%.

Plastic Surgeons as Medical Directors: A Natural Transition into Medical Leadership

  • Jalalabadi, Faryan;Ferry, Andrew M.;Chang, Andrew;Reece, Edward M.;Izaddoost, Shayan A.;Hassid, Victor J.;Tahiri, Youssef;Buchanan, Edward P.;Winocour, Sebastian J.
    • Archives of Plastic Surgery
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    • v.49 no.2
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    • pp.221-226
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    • 2022
  • With the growing complexity of the U.S. health care system, highly motivated medical directors with strong leadership skills are vital to the success of health care facilities. Presently, there are no articles assessing a plastic surgeon's qualifications for the role of medical director. In addition, there is a paucity of literature comparing the responsibilities of medical directors across various types of health care institutions. Herein, we outline why plastic surgeons have the unique skillset to succeed in this role and highlight the differences between medical director positions across the vast landscape of health care. While the intricacies of this position vary greatly across different landscapes of the health care industry, successful medical directors lead by following a set of universal principles predisposing them for success. Plastic surgeons innately exhibit a subset of particular traits deeming them suitable candidates for the medical director position. While transitioning from the role of a surgeon to that of a medical director does require some show of adaptation, plastic surgeons are ultimately highly likely to find intrinsic benefit from serving as a medical director.

Prognostic Impact of Postoperative Complications in High-Risk Operable Non-small Cell Lung Cancer

  • Lee, Seungwook;Roknuggaman, Md;Son, Jung A;Hyun, Seungji;Jung, Joonho;Haam, Seokjin;Yu, Woo Sik
    • Journal of Chest Surgery
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    • v.55 no.1
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    • pp.20-29
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    • 2022
  • Background: Patients with high-risk (HR) operable non-small cell lung cancer (NSCLC) may have unique prognostic factors. This study aimed to evaluate surgical outcomes in HR patients and to investigate prognostic factors in HR patients versus standard-risk (SR) patients. Methods: In total, 471 consecutive patients who underwent curative lung resection for NSCLC between January 2012 and December 2017 were identified and reviewed retrospectively. Patients were classified into HR (n=77) and SR (n=394) groups according to the American College of Surgeons Oncology Group criteria (Z4099 trial). Postoperative complications were defined as those of grade 2 or higher by the Clavien-Dindo classification. Results: The HR group comprised more men and older patients, had poorer lung function, and had more comorbidities than the SR group. The patients in the HR group also experienced more postoperative complications (p≤0.001). More HR patients died without disease recurrence. The postoperative complication rate was the only significant prognostic factor in multivariable Cox regression analysis for HR patients but not SR patients. HR patients without postoperative complications had a survival rate similar to that of SR patients. Conclusion: The overall postoperative survival of HR patients with NSCLC was more strongly affected by postoperative complications than by any other prognostic factor. Care should be taken to minimize postoperative complications, especially in HR patients.

Endoscopic Retrograde Cholangiopancreatography in Bangladeshi Children: Experiences and Challenges in a Developing Country

  • Rashid, Rafia;Arfin, Md. Samsul;Karim, A.S.M. Bazlul;Alam, Muhammad Baharul;Mahmud, Salahuddin
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.25 no.4
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    • pp.332-339
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    • 2022
  • Purpose: Although endoscopic retrograde cholangiopancreatography (ERCP) has been used for more than five decades, its applicability in Bangladeshi children has recently become more common. Therefore, this manuscript aims to describe our experience in performing ERCPs in Bangladeshi children with hepatopancreaticobiliary diseases, focusing on presenting diseases, as well as the diagnostic and therapeutic efficacy. Methods: Between 2018 and 2021, 20 children underwent 30 ERCP procedures at the Bangladesh Specialized Hospital, Dhaka. A single trained adult gastroenterologist performed all procedures using a therapeutic video duodenoscope. The indications for ERCP, diagnostic findings, therapeutic procedures, and complications were documented. Results: The median age of the study patients was 10 years (range, 1.7-15 years). Successful cannulation of the papilla was achieved in 28 procedures and failed in 2 cases. Repeated ERCP was required in seven patients. Nine patients had biliary indications and 11 had pancreatic indications. Choledocholithiasis was the most common indication for ERCP in patients with biliary disease, while chronic pancreatitis was common among patients with pancreatic indications. Pancreatic divisum was observed in only one patient. Pancreatic and biliary sphincterotomy was performed in 14 and 9 cases, respectively. A single pigtail or straight therapeutic stent was inserted in seven cases and removed in five cases. Stone extraction was performed in six procedures, and balloon dilatation was performed in five procedures. The post-procedural period for these patients was uneventful. Conclusion: We found that ERCP is a practical and successful therapeutic intervention for treating hepatopancreaticobiliary disorders in children when performed by experienced endoscopists.