• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.544-550
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• 2005

To develop a highly efficient mammalian expression vector, a series of vectors were constructed based on the murine cytomegalovirus (MCMV) immediate-early (IE) promoter and human ${\beta}$-globin second intron. The resulting MCMV promoter was several-fold stronger than the HCMV promoter in various mammalian cell lines, such as the NIH3T3, Neuro-2a, 293T, and HT1080 cell lines, and was only slightly weaker than the HCMV promoter in HeLa and CHO cells. The inclusion of the human ${\beta}$-globin second intron behind the MCMV promoter or HCMV promoter markedly enhanced the promoter activity in various mammalian cell lines, and the resultant MCMV/Glo-I expression system was stronger than the HCMV promoter from 4.7- to 11.2-fold in every cell line tested. Also, the MCMV/Glo-I promoter induced a higher level of the VSV-G protein in a transiently transfected 293T cell line, which is useful for the production of recombinant retrovirus and lentivirus vectors.

• Korean Journal of Microbiology
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• v.27 no.2
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• pp.85-91
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• 1989

The effect of SV40 and murine cytomegalovirus (MCMV) enhancers on the general and tissue-specific gene expression was investigated. Recombinant plasmids containing these transcriptional engancers downstream of a structural gene for chloramphenicol acetyl transferase (CAT) were constructed. The transient expression of CAT gene from these plasmids was measured in monkey (CV1PD) and HeLa cells. Both SV40 and MCMV engancers activated the expression of CAT gene by more than 20 and 150 folds, respectively, compared with engancerless plasmids. When the SV40 promoter, upstream of CAT gene, was replaced with 2.2 kbp of promoter regulatory region of bovine growth hormone (bGH) gene, there was no expression of CAT even in the presence of enhancers, reflecting the tissue-specific expression of bGH genes. However, when the bGH regulatory region was shortened to 230 bp, the expression level increased dramatically in the presence of SV40 enhancers. In contrast, the expression from the shortened promoter was only marginally activated by the stronger MCMV enhancer.

• Defense and Technology
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• no.11 s.177
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• pp.32-37
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• 1993

기뢰전 함정(MCMV)의 전투체계는 지휘통제체계를 중심으로 센서, 항해체계, 기뢰제거 시스템을 분산식 구성개념으로 통합하여 모든 하위체계 자료와 지휘통제용 다기능콘솔 사이의 자료를 고속의 데이터버슬 통해 교환하는 것이 일반적인 추세입니다 특히, 원격조종 수중운반체에 센서와 기뢰제거 장치를 탑재하여 전방에 전개시켜 기뢰전 함정 자체의 안전성을 높여줌은 물론, 수중 또는 해저에 부설된 기뢰의 탐지와 식별 무력화 성능을 높여주고 있습니다