• Journal of Aquaculture
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• v.14 no.1
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• pp.57-64
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• 2001

In Paralichthys olivaceus and Nibea japonica, response to live air (5hr) or ship (25hr) transportation was assessed, by determining the levels of plasma cortisol, glucose, lactic acid and osmolality, as well as hematological parameters namely hematocrit (Ht), red blood cell (RBC) count, hemoglobin (Hb), corpuscular volume (MCV), corpuscular hemoglobin (MCH) and corpuscular hemoglobin concentration (MCHC). In the experimental series I, the olive flounder was subjected to stress or no stress for 1hr, prior to its air transportation for 5hr. The stress suffered by the flounder prior to air transportation resulted in significant reduction in Ht, but increases in MCH and MCHC. Air transportation led to increases in MCV and MCH in both the stressed and non stressed groups. In the non stressed group it led to significant increase in Ht but decrease in MCHC. In the stressed group, the air transportation led to significant increases in osmolality and plasma cortisol from 5 to 38.5ng/$m\ell$. Non stressed groups did not show significant differences in this before and after transportation. In the experiment II, the red blood cell (RBC) count ranged from 2.5$\times$10$^{6}$ /${mu}ell$ to 2.7$\times$10$^{6}$ /${mu}ell$ in the flounder and 1.9$\times$10$^{6}$ /${mu}ell$ to 2.1$\times$10$^{6}$ /${mu}ell$ in the croaker during the pre- and post-transportation, respectively. In the croaker the shipping led to significant increase in plasma cortisol from 26 to 35ng/$m\ell$ but decrease in glucose from 91.0 to 26.4mg/㎗. For glucose the reverse (39.0 to 51.0mg/㎗) was true for the flounder.

• Korean Journal of Veterinary Research
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• v.30 no.2
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• pp.157-162
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• 1990

The hematologic values were examined from 74 healthy mixed breed dogs in the area of Seoul. The results obtained are summarized as follows; 1. Mean${\pm}$SD values and ranges of red blood cell(RBC) count were $6.16{\pm}0.92{\times}10^6/{\mu}l$ and $4.40{\sim}8.62{\times}10^6/{\mu}l$, of hemoglobin(Hb) content $14.90{\pm}2.42g/100ml$ and 8.7~19.2g/100ml, of packed cell volume(PCV) $45.47{\pm}6.16ml/100ml$ and 30~57ml/100ml, of mean corpuscular volume (MCV) $74.80{\pm}6.83fl$ and 54.08~90.90fl, of mean corpuscular hemoglobin (MCH) $24.41{\pm}3.91pg$ and 14.19~32.97pg, of mean corpuscular hemoglobin concentration (MCHC) $32.07{\pm}3.24g/100ml$ and 22.23~39.76g/100ml, respectively. 2. The RBC count value in the age group of 3~4 years was higher (p<0.05) than the total RBC count value. The Hb content value in the age group of less than 6 months was lower (p<0.01) but in the age group of 1~2 years and the age group of 3~4 years were higher (p<0.05, p<0.01) than the total Hb content value. The PCV value in the age group of less than 6 months was lower (p<0.05) than the total PCV value. The MCHC values in the age group of 1~2 years and the age group of 3~4 years were higher (p<0.01, p<0.05) than the total MCHC value. 3. Mean${\pm}$SD values and ranges of white blood cell (WBC) count were $11.26{\pm}3.05{\times}10^3/{\mu}l$ and $6.30{\sim}18.4{\times}10^3/{\mu}l$, of band neutrophil $2.97{\pm}1.44%$ and 1~10%, of segmented neutrophil $62.81{\pm}4.92%$ and 42~70, of lymphocyte $30.55{\pm}5.69%$ and 17~50%, of monocyte $2.49{\pm}0.84%$ and 1~5%, of eosinophil $1.81{\pm}1.175$ and 1~8%, respectively. 4. The WBC count value in the age group of 7~12 months was lower (p<0.05) but in the age group of 3~4 years was higher (p<0.05) than the total WBC count value. The band neutrophil values in the age group of 1~2 years and the age group of 3~4 years were higher (p<0.05, p<0.01) than the total band neutrophil value.

• Korean Journal of Veterinary Service
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• v.14 no.1
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• pp.13-17
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• 1991

This studies were carried out to investigate the changes of blood pictures within 24hrs after birth in Korean native calves. The hematologic values were examined from 300 heads healthy Korean native calves in the area of western farm of Chungnam province. The results obtained are summarized as follows : 1. $Mean{\pm}SD$ values of serum total protein contents within 24 hrs after birth in Korean native calves were $8.42{\pm}0.92g$ / dl and 48.14{\pm}0.93g$ / dl, respectively. 2. $Meant{\pm}SD$ values of RBC count, WBC count, PCV and Hb contents within 24 hrs after birth in Koren native natives were 49.97{\pm}2.56{\times}10^3$ / mm^3$ and $9.88{\pm}2.93{\times}10^3/ mm^3, $ 439.56{\pm}15.42%$ and 441.66{\pm}7.09 %, $$ 12.79{\pm}1.59g$ / dl and 13.$63{\pm}1.87g$ / dl, respectively. 3. 4Meant{\pm}SD$ values of MCV, MCH and MCHC within 24 hrs after birth in Koren native calves were $43.41{\pm}3.09{\mu}mm^3$ and $43.88{\pm}3.31{\mu}mm^3, $ $14.05{\pm}1.22pg $and 414.36{\pm}1.6pg, $ $32.46{\pm}2.47g$ / dl and 432.96{\pm}3.17g$ / dl, respectively.

• Korean Journal of Veterinary Research
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• v.46 no.4
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• pp.387-393
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• 2006

Progressing to the iron deficiency anemia was experimentally induced in 4 clinically healthy dogs by repeated phlebotomy to characterize hematologic features, serum iron values, and RBC indices. Abnormal RBC morphologies were also evaluated semiquantitatively on Wright's-stained blood films. Hematologic abnormalities in early stage of anemia included decreased both hematocrit and hemoglobin, and reticulocytosis, with no changes in mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) were represented. In intermediate stage, decreased serum iron concentration with microcytosis and hypochromia were prominent. In late stage, red cell distribution width and Mentzer's index were out of reference ranges in the majority of dogs. In this study microcytic anemia was appeared at the hemoglobin range of 5.1-7.2 g/dl. On most sampling days, platelet counts and white blood cells were within the reference ranges, with some minor variations. Iron deficiency was not necessarily associated with microcytic anemia. Judging from the sequential changes of both MCV and MCHC, 3 patterns of anemia were sequentially observed: initially normocytic normochromic, intermediate normocytic hypochromic or normocytic normochormic, and finally microcytic hypochromic. The most frequent morphologic abnormalities were target cells. Occasional elliptocyte, acanthocyte, stomatocyte, kinzocyte, dacrocyte and schistocyte were also noted on the blood films.

• Toxicological Research
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• v.23 no.4
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• pp.391-403
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• 2007

The study was conducted to assess the repeated dose and reproduction and developmental toxicities of acetanilide, an intermediate for drug production, as a part of OECD Screening Information Data Set (SIDS) program. The test agent was administered by gavage at dose levels of 0, 22, 67, 200 and 600 mg/kg to Sprague-Dawley rats (12/group/sex) during pre-mating and mating period for males(up to 30 days) and females and pregnancy and early lactation period for females (up to 39-50 days). At 22 mg/kg, decreases in HGB, HCT (males) and MCHC (females), hyperplasia of spleen red pulp, hyperplasia of femur bone marrow (both sexes) were observed. At 67 mg/kg, salivation (males), reduced food consumption (both sexes), decreases in RBC, HGB, HCT and MCHC (males), increases in MCV (males) and spleen weight (males), hyperplasia of spleen red pulp and femur bone marrow (both sexes) were observed. At 200 mg/kg, decreases in locomotor activity and salivation (both sexes), reduced food consumption (both sexes), decreases in RBC, HGB, HCT and increases in MCV, MCH, BUN, T-BIL (males), enlargement of spleen (both sexes), increased weight of spleen (males), hyperplasia of spleen red pulp and femur bone marrow and extramedullary hematopoiesis of liver (both sexes), atrophy of thymus and corpus luteum hyperplasia of ovary (females) were observed. At 600 mg/kg, decreases in locomotor activity, cyanosis (both sexes), reddish tear, and salivation (males), mortality (4 out of 12 females), decreased body weight (females), reduced food consumption (both sexes), decreases in RBC, HGB, HCT and MCHC and increases in WBC, MCV, MCH, reticulocyte, neutrophil, lymphocyte, monocyte, AST, ALT, BUN, T-BIL, ALB, Ca and A/G ratio (males), enlargement of spleen, increased weights of spleen (both sexes), liver (males), kidney and ovary, decreased weights of thymus (females), hyperplasia of spleen red pulp, hyperplasia of femur bone marrow and extramedullary hematopoiesis of liver (both sexes), and atrophy of thymus and corpus luteum hyperplasia of ovary (females) were observed. Regarding the reproduction and development toxicities, there were no treatment-related changes in precoital time, mating index, fertility index and pregnancy index at all doses tested. At 22 and 67 mg/kg, there were no adverse effects on all the parameters observed. At 200 mg/ kg, decreased body weight of pups (day 4 p.p.) were observed. At 600 mg/kg, decreased body weight of pups (day 0 and 4 p.p.) and viability index (day 4 p.p.), increased incidence of newborns dead or with abnormal clinical signs were observed. The results suggest that the NOAELs for general toxicity are < 22 mg/kg, LOAELs are 22 mg/kg and the NOAELs for reproductive toxicity are 67 mg/kg.

• Korean Journal of Community Nutrition
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• v.2 no.5
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• pp.687-694
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• 1997

This study investigated the effects of vitamin C supplementation on the nutritional iron status of 31 adolescent girls, aged 12-15 years, with low hemoglobin levels. They were randomly divided into four groups, and for two groups daily150mg or 900mg of L-ascorbic acid(AsA) was given in three equal doses at three meals during 9 weeks. To another group daily 60mg iron as ferrous sulfate was given in the same way as AsA. The control group was given sugar placebo. Body iron status was monitored through the determination of Hb, Hct, MCHC, and serum ferritin concentrations. Dietary AsA and iron intakes were measured from food consumption surveys performed by 3-day 24-hour recalls. The amount of absorbed iron was estimated from the model of Monsen et al. The average amounts of food iron for four groups were 12.3- 15.0mg and 11.1 - 18.9mg at initial and at final period of the supplementation trial, respectively. The tentatively estimated amount of absorbed iron was significantly increased in the 900mg AsA and iron supplementing groups, but not in the 150mg AsA and placebo groups. Both Hb and MCHC were improved to above normal levels in all groups except the placebo group. Hct was elevated only in the AsA 900mg group whose Hct was relatively lower than the other groups. Serum ferritin concentrations of the four groups, which were as low as 8.50 - 14.39ng/mL on average at the intial periods, augmented significantly to 20.18ng/mL and 26.63ng/mL in the 900mg AsA and iron groups, respectively. Serum ferritin was not elevated in either the AsA 300mg group or the placebo group. The above data indicated that the daily supplementaion of 150mg AsA to the meals containing 12-15mg iron per day promoted Hb levels of adolescent girls with low Hb, and the 900mg AsA supplementing improved not only Hb level but also body iron store. A supplementation of 60mg iron per day appeared to be slightly more effective in improving the iron status in comparison to the 900mg AsA supplement. (Korean J Community Nutrition 2(5) : 687-694, 1997)

• Toxicological Research
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• v.7 no.1
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• pp.51-59
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• 1991

The purpose of the present study was to investigate the effects of non-watering, fasting and non-watering fasting for 16-17 hrs on haematological and biochemical parameters in SD rats. 1. Liver weight was decreased in the order of in the fasting group, non-watering fasting group and non-watering group in both sexes. 2. In fasting group and non-watering fasting group, haematologic parameters of male (HGB and MCH) and femal (MCHC) were trends to decrease compared with normal feeding & watering group. 3. In the differential leucocyte counts, there were no significant differences compared with noraml feeding % watering group.

• Journal of Veterinary Clinics
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• v.17 no.2
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• pp.346-348
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• 2000

In previous studies, high fever, inappentence, increased respiratory rate, muscle tremors, anemia, jaundice, loss of weight hemoglobinenuria and hemoglobinuria were found in dogs infected intraerythrocytic protzoan parasites of the gennus Babesia. These changes were mainly caused by the intravascular hemolysis. In this study, a Pit Bull Terrier, which was infected by babesia, was investigated clinical signs and patterms of blood. The findings from this study indicated that dog with severs clinical signs shown erythropenia, lymphocytophilia, neutropenia, thrombocytopenia and eosinpenia. Furthermore, it presenter low level of hemglobin and hematocrit. However MCV, MCH and MCHC did not decreased as compare to normal dogs.

• Development and Reproduction
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• v.21 no.3
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• pp.249-257
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• 2017

In a 12-week experiment, the rock bream, Oplegnathus fasciatus, and olive flounder, Paralichthys olivaceus, were investigated to determine the effects of starvation on their physiological parameters. The protein and DNA contents of the starved fish were significantly higher than the initial values and those of the fed fish. The RNA contents and RNA/DNA ratios of the fed fish were significantly higher than those of the other groups (P<0.05). The hematocrit, hemoglobin, red blood cells (RBC), and mean corpuscular volume (MCV) of the fed rock bream were significantly higher than at baseline (P<0.05), whereas the mean corpuscular hemoglobin concentration (MCHC) of the fed fish was lower than at baseline (P<0.05). The hematocrit, hemoglobin, RBC, and MCHC of the starved group were significantly lower than the baseline values, whereas the MCV of the starved group was significantly higher than the baseline value (P<0.05). No significant difference in alanine aminotransferase was observed between the fed fish and baseline, whereas the starved fish value was significantly higher than the baseline value (P>0.05). There were no significant differences in cortisol levels. However, the glucose level in the fed group was significantly higher than the baseline level and that in the starved group was significantly lower than the baseline level (P<0.05).

• Korean Journal of Veterinary Service
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• v.28 no.1
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• pp.39-47
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• 2005

A survey on the prevalence of heartworms, scabies and demodicosis of 1,011 pet dogs in 5 animal hospitals Jeonju area was peformed from June to October 2004. The result obtained from this experiment were summarized as follows; 1. The 1,011 pet dogs coming to help the animal hospital more than disease of pleonephritis 62($6.1\%$), enteritis 75($7.4\%$), dermatitis 62($6.1\%$), scabies 55($5.4\%$), heartworms 43($4.3\%$), tympanitis 41($4.1\%$), demodicosis 22($2.2\%$), gastritis 21($2.2\%$), fracture 9($0.9\%$), respectively. 2. The 307 examined pet dogs were heartworms antigen positive to 43($14.0\%$), Microfilaria 31($10.1\%$) and the kinds of mixed breeds 34 (antigen and Microfilaria 12), Jindo 19 (antigen 7, Microfilaria 6), respectively. 3. Heartworms antigen positive of examined 34 pet dogs mean ages 4.3 and general blood cell count mean of WBC $20.5{\times}10^3/mm^3$, RBC $61.3{\times}10^6/mm^3$ HGB $6.27g/d{\ell}$ HCT $20.9\%$, PLT $13.0{\times}10^3/mm^3$, MCV $33.2{\mu}m^3$, MCH 39.4pg, MCHC $14.8g/d{\ell}$, RDW $280.1\%$, MPV $9.2{\mu}m^3$, respectively. but showed a few high normal value WBC, MCV, MCH and a low MCHC. 4. This experiment was 1,011 pet dogs to dermatitis 139($13.7\%$) of scabies 55($39.6\%$), demodicosis 22($15.8\%$), respectively.