• 생명과학회지
• /
• 제30권5호
• /
• pp.468-475
• /
• 2020

본 연구에서는 홍국색소의 항산화 활성 및 조골세포의 분화에 미치는 영향을 검토하였다. 홍국색소의 항산화 활성은 DPPH radical 소거능, ABTS radical 소거능 및 SOD 유사 활성을 측정하여 평가하였다. 홍국색소의 DPPH radical 및 ABTS radical 소거 활성은 농도 의존적으로 증가하였으며, 특히 1,000 ㎍/ml 농도에서 각각 94% 및 99%의 최대 활성을 나타내었다. 또한 SOD 유사 활성을 검토한 결과에서, 홍국색소 1,000 ㎍/ml 농도에서 62%의 최대 활성을 나타내었다. 우선 홍국색소 1~1,000 ㎍/ml의 농도에서 세포독성을 검토하기 위해 MTT assay를 실시한 결과, 1~100 ㎍/ml 농도의 범위에서 세포 독성은 나타나지 않았다. ALP 활성은 1~100 ㎍/ml 농도에서 농도의 존적으로 증가하였으며, 100 ㎍/ml의 농도에서 최대 124%의 활성을 나타내었다. 또한, 석회화 형성능 시험에서도 대조군 대비 모든 농도에서 유의적으로 증가하는 경향이 나타났으며, 100 ㎍/ml의 농도에서 대조군 대비 1.5배의 석회화 형성 결과가 나타났다. 이러한 결과를 통해 홍국색소는 항산화 활성이 우수하며, 조골세포 분화에 긍정적인 영향을 줌으로써 골다공증 예방 소재로서의 개발 가능성을 가진 부가가치가 높은 천연물 소재로 활용 가능할 것으로 생각된다.

• PNF and Movement
• /
• 제20권2호
• /
• pp.189-201
• /
• 2022

Purpose: The purpose of this study was to investigate the effect of McConnell taping and Kinesio taping on pain and lower extremity joint angles when patients with patellofemoral pain syndrome (PFPS) ascend stairs. Methods: Fifty young adults who were experiencing anterior knee pain due to PFPS were selected as participants. Then, 25 patients were randomly assigned to the McConnell taping group and 25 to the Kinesio taping group. Pain and lower extremity joint angle were measured while ascending stairs before and after the intervention. A paired t-test was performed to evaluate the amount of change in the parameter values after the intervention within the groups, and an independent t-test was used to compare the results of the groups. Results: In the within-group comparisons, a statistically significant difference was found in both groups between the anterior knee pain scale scores recorded before and after the intervention (p < 0.05). A statistically significant difference was also found between the groups (p < 0.05). Comparison of the lower extremity joint angles at initial contact, loading response, terminal stance, and pre-swing within the groups showed that there were statistically significant differences in the hip, knee flexion, abduction, lateral rotation, and dorsiflexion angles in both the McConnell and Kinesio taping groups (p < 0.05). There was also a statistically significant difference in all angles between the groups during the following events (p < 0.05): (1) at initial contact, (2) at loading response (except hip flexion angle), (3) at terminal stance (except hip flexion and lateral rotation angles), and (4) at pre-swing (except hip, knee abduction, and inversion angles). Conclusion: McConnell taping and Kinesio taping both effectively improved the occurrence of knee pain and the lower extremity joint angles during stair ascent in patients with PFPS. However, McConnell taping had a significant impact on pain reduction and lower extremity joint angles compared to Kinesio taping.

• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 2003년도 Annual Meeting of KSAP : International Symposium on Pharmaceutical and Biomedical Sciences on Obesity
• /
• pp.109-109
• /
• 2003

Taurine is present in a variety of tissue and exhibits many important physiological functions in many tissues. Although it is known that many tissues mediate taurine transport, its functions of taurine transport in bone have not been identified yet. In the present study, we investigated the expression of taurine transporter (TauT) and taurine uptake using mouse stromal ST2 cells and osteoblast-like MC3T3-El cells, which is bone related cells. Detection of TauT mRNA expression in these cells were performed by reverse transcription polymerase chain reaction (RT-PCR). The activity of TauT was assessed by measuring the uptake of ［$^3$H］taurine in the presence or absence of inhibitors. TauT mRNA was detected in these cells. ［$^3$H］Taurine uptake was dependent upon the presence of extracellular sodium, chloride and calcium ions, and inhibited by cold-taurine and ${\beta}$-alanine. These results suggest that taurine has biological functions in bone and some effect on the bone cells.

• Journal of Periodontal and Implant Science
• /
• 제42권3호
• /
• pp.95-104
• /
• 2012

Purpose: The purpose of this study was to determine whether increasing the Ti6Al4V surface oxide negative charge through heat ($600^{\circ}C$) or radiofrequency plasma glow discharge (RFGD) pretreatment, with or without a subsequent coating with fibronectin, stimulated osteoblast gene marker expression in the MC3T3 osteoprogenitor cell line. Methods: Quantitative real-time polymerase chain reaction was used to measure changes over time in the mRNA levels for osteoblast gene markers, including alkaline phosphatase, bone sialoprotein, collagen type I (${\alpha}1$), osteocalcin, osteopontin and parathyroid hormone-related peptide (PTH-rP), and the osteoblast precursor genes Runx2 and osterix. Results: Osteoprogenitors began to differentiate earlier on disks that were pretreated with heat or RFGD. The pretreatments increased gene marker expression in the absence of a fibronectin coating. However, pretreatments increased osteoblast gene expression for fibronectin-coated disks more than uncoated disks, suggesting a surface oxide-mediated specific enhancement of fibronectin's bioactivity. Heat pretreatment had greater effects on the mRNA expression of genes for PTH-rP, alkaline phosphatase and osteocalcin while RFGD pretreatment had greater effects on osteopontin and bone sialoprotein gene expression. Conclusions: The results suggest that heat and RFGD pretreatments of the Ti6Al4V surface oxide stimulated osteoblast differentiation through an enhancement of (a) coated fibronectin's bioactivity and (b) the bioactivities of other serum or matrix proteins. The quantitative differences in the effects of the two pretreatments on osteoblast gene marker expression may have arisen from the unique physico-chemical characteristics of each resultant oxide surface. Therefore, engineering the Ti6Al4V surface oxide to become more negatively charged can be used to accelerate osteoblast differentiation through fibronectin-dependent and independent mechanisms.

• The Korean Journal of Physiology and Pharmacology
• /
• 제9권1호
• /
• pp.55-62
• /
• 2005

Very little research has been carried out on safflower seed for the prevention and treatment of the bone deficiency diseases, including osteoporosis, which are supported by scientific evidences. In the present study, $3{\mu}l$ of 0.1% dried crude extract or $2{\mu}l$ of 0.1% dried aqueous fraction were shown to significantly accelerate the rate of differentiation of osteoblast. Also, the crude extract and aqueous fraction increased the $[Ca^{2+}]_i$ of the cultured osteoblast cells: $3{\mu}l$ of 0.1% dried crude extract and $2{\mu}l$ of 0.1% dried aqueous fraction significantly increased the $[Ca^{2+}]_i$ of the cultured osteoblast cells ($8{\times}10^{-4}$) to the extent that it deserves a considerable attention. Furthermore, the crude extract and aqueous fraction increased the $[Ca^{2+}]_i$ of the cultured osteoblast cells, and $300{\mu}M$ $Cd^{2+}$, specific calcium channel blocker, completely blocked the increase. Therefore, the increased $[Ca^{2+}]_i$ of the cultured osteoblast cells by safflower seed component continued to activate calcium channel.

• The Journal of Advanced Prosthodontics
• /
• 제6권3호
• /
• pp.157-164
• /
• 2014

PURPOSE. This study focused on in vitro cell differentiation and surface characteristics in a magnesium coated titanium surface implanted on using a plasma ion source. MATERIALS AND METHODS. 40 commercially made pure titanium discs were prepared to produce Ti oxide machined surface (M) and Mg-incorporated Ti oxide machined surface (MM). Surface properties were analyzed using a scanning electron microscopy (SEM). On each surface, alkaline phosphatase (ALP) activity, alizarin red S staining for mineralization of MC3T3-E1 cells, and quantitative analysis of osteoblastic gene expression, were evaluated. Actin ring formation assay and gene expression analysis of TRAP and GAPDH performing RT-PCR were performed to characterize osteoclast differentiation on mouse bone marrow-derived macrophages (BMMs). RESULTS. MM showed similar surface morphology and surface roughness with M, but was slightly smoother after ion implantation at the micron scale. M was more hydrophobic than MM. No significant difference between surfaces on ALP activity at 7 and 14 days were observed. Real-time PCR analyses showed similar levels of mRNA expression of the osteoblast phenotype genes; osteopontin (OPN), osteocalcin (OCN), bone sialoprotein (BSP), and collagen 1 (Col 1) in cell grown on MM at 7, 14 and 21 days. Alizarin red S staining at 21 days showed no significant difference. BMMs differentiation increased in M and MM. Actin ring formation assay and gene expression analysis of TRAP showed osteoclast differentiation to be more active on MM. CONCLUSION. Both M and MM have a good effect on osteoblastic cell differentiation, but MM may speed the bone remodeling process by activating on osteoclast differentiation.

• Journal of Communications and Networks
• /
• 제7권2호
• /
• pp.157-170
• /
• 2005

The combination of multiple antennas and multi-carrier code division multiple-access (MC-CDMA) is a strong candidate for the downlink of the next generation mobile communications. The study of such systems in scenarios that model real-life trans-missions is an additional step towards an optimized achievement. We consider a realistic MIMO channel with two or four transmit antennas and up to two receive antennas, and channel state information (CSI) mismatches. Depending on the mobile terminal (MT) class, its number of antennas or complexity allowed, different data-rates are proposed with turbo-coding and asymptotic spectral efficiencies from 1 to 4.5 bit/s/Hz, using three algorithms developed within the European IST-MATRICE project. These algorithms can be classified according to the degree of CSI at base-station (BS): i) Transmit space-frequency prefiltering based on constrained zero-forcing algorithm with complete CSI at BS; ii) transmit beamforming based on spatial correlation matrix estimation from partial CSI at BS; iii) orthogonal space-time block coding based on Alamouti scheme without CSI at BS. All presented schemes require a reasonable complexity at MT, and are compatible with a single-antenna receiver. A choice between these algorithms is proposed in order to significantly improve the performance of MC-CDMA and to cover the different environments considered for the next generation cellular systems. For beyond-3G, we propose prefiltering for indoor and pedestrian microcell environments, beamforming for suburban macrocells including high-speed train, and space-time coding for urban conditions with moderate to high speeds.

• 한국영양학회:학술대회논문집
• /
• 한국영양학회 2004년도 춘계학술대회
• /
• pp.112-112
• /
• 2004

• 대한치주과학회:학술대회논문집
• /
• 대한치주과학회 2001년도 제41회 종합학술대회 연제초록
• /
• pp.44-45
• /
• 2001

• 대한치주과학회:학술대회논문집
• /
• 대한치주과학회 2001년도 제41회 종합학술대회 연제초록
• /
• pp.46-46
• /
• 2001