• 농업과학연구
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• 제39권2호
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• pp.211-217
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• 2012

There is an emerging interest in using DNA markers for breed identification in animals. This article reviews the breed identification markers in chicken, mainly developed in Chungnam National University, with particular emphasis on the mitochondrial DNA markers and the nuclear DNA markers including the SNPs in MHC region and the plumage color related MC1R markers. This information would be very useful for an appropriate conservation breeding program as well as for the establishment of molecular markers for chicken breed identifications.

• 한국임상수의학회:학술대회논문집
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• 한국임상수의학회 2009년도 추계학술대회
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• pp.254-254
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• 2009

• 대한화학회지
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• 제53권6호
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• pp.742-748
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• 2009

정육 사업에서 고기의 원산지와 종을 표기 하는 것은 육질의 판단에 영향을 미친다. 유전자 마커는 종을 판별하기 위한 증거로 사용되므로, 우리는 한우와 젖소 그리고 혼입육을 판단 할 수 있는 유전자 마커의 개발 계획을 수립하였다. 소의 모든 종은 모색 유전자에 의하여 그 종이 결정 되며 모색 유전자의 조절에 의하여 유멜라닌 또는 페오멜라닌이 합성되고, 이로 인하여 모색에 차이가 생기는 점을 이용하여, 종을 판별하는 유전자 마커로 사용된다. 암소와 수소를 판별하기 위하여 Y 염색체 상에 존재하는 성 결정 부위 유전자에 대응하는 프라이머를 제작하였다. 본 연구에서는 모색 유전자와 성 결정 유전자를 다중 중합효소연쇄반응(multiplex-PCR)을 이용하여 증폭하였고, 증폭 산물을 제한 효소 MspA1I로 소화 시켰다. 이 반응물을 변성 고성능 액체 크로마토그래피(denaturing high performance liquid chromatography, DHPLC)를 이용하여 분석하였다. 분석 결과 6가지의 크로마토그램을 확인 할 수 있었고, DHPLC 분석 방법은 한우, 젖소 그리고 혼입육을 쉽게 구별해 낼 수 있었다.

• 한국수정란이식학회지
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• 제30권3호
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• pp.183-188
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• 2015

본 연구는 제주마 집단의 모색유전자 분포 특성 및 세대별 모색 유전자 변화 양상을 분석하기 위해 제주특별자치도에서 관리하는 제주마, 1,462두에 대한 모색 관련 유전자인 MC1R, ASIP, ECA3-inversion, STX17을 분석하였다. 흑모색과 관련된 MC1R 유전자의 $E^+/E^+$와 $E^+/E^e$ 유전자형 빈도는 각각 0.122와 0.447로 조사되었고, 적색(chestnut) 유전자형인 $E^e/E^e$ 유전자형 빈도는 0.429로 확인되었다. ASIP 유전자의 $A^A/A^A$, $A^A/A^a$ 및 $A^a/A^a$ 유전자형 빈도는 각각 0.46, 0.448, 0.091로 확인되어 $A^a/A^a$ 유전자형 빈도가 낮았다. 토비아노(Tobiano) 형태를 발현하는 To/To와 +/To 유전자형은 각각 0.001과 0.119로 토비아노 형태의 모색은 12%를 점유하고 있었다. 회색 모색과 관련된 STX17의 G/G와 G/g 유전자형의 빈도는 각각 0.002와 0.680로 나타나 제주마 집단에 회색마는 68.2%를 점유하였다. 세대별 모색 변화는 MC1R, ASIP 유전자에서는 큰 변화를 확인할 수 없었다. ECA3-inversion에서는 토비아노를 발현하는 To allele은 세대변화에 따라 유의적으로 감소하였고(p<0.05), 회색 모색과 관련된 STX17 G allele은 세대변화에 따라 유의적으로 증가하였다(p<0.05). 본 연구결과, 제주마 집단의 모색 다양성을 유지하기 위해서는 종마 선정 단계에 모색 유전자의 검토가 필요한 것으로 사료되었다.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.85.3-86
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• 2003

Oltipraz, a cancer chemopreventive agent, induces CYP1A1 to a certain extent by transactivation of the gene via the Ah receptor (AhR)-xenobiotic response element (XRE) pathway. Previously, we showed that oltipraz promoted CCAAT/enhancer binding protein (C/EBP ) activation, which leads to the induction of glutathione S-transferase. Given that oltipraz activates C/EBP for gene transactivation and that the putative C/CBP binding site is located in CY)1A1 promoter region, this study investigated the effect of oltipraz on CYP1A1 induction by 3-methylcholanthrene (3-MC). (omitted)

• Restorative Dentistry and Endodontics
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• 제39권3호
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• pp.187-194
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• 2014

Objectives: The effects of bone morphogenetic protein-2 (BMP-2) and enamel matrix derivative (EMD) respectively with mineral trioxide aggregate (MTA) on hard tissue regeneration have been investigated in previous studies. This study aimed to compare the osteogenic effects of MTA/BMP-2 and MTA/EMD treatment in MC3T3-E1 cells. Materials and Methods: MC3T3-E1 cells were treated with MTA (ProRoot, Dentsply), BMP-2 (R&D Systems), EMD (Emdogain, Straumann) separately and MTA/BMP-2 or MTA/EMD combination. Mineralization was evaluated by staining the calcium deposits with alkaline phosphatase (ALP, Sigma-Aldrich) and Alizarin red (Sigma-Aldrich). The effects on the osteoblast differentiation were evaluated by the expressions of osteogenic markers, including ALP, bone sialoprotein (BSP), osteocalcin (OCN), osteopontin (OPN) and osteonectin (OSN), as determined by reverse-transcription polymerase chain reaction analysis (RT-PCR, AccuPower PCR, Bioneer). Results: Mineralization increased in the BMP-2 and MTA/BMP-2 groups and increased to a lesser extent in the MTA/EMD group but appeared to decrease in the MTA-only group based on Alizarin red staining. ALP expression largely decreased in the EMD and MTA/EMD groups based on ALP staining. In the MTA/BMP-2 group, mRNA expression of OPN on day 3 and BSP and OCN on day 7 significantly increased. In the MTA/EMD group, OSN and OCN gene expression significantly increased on day 7, whereas ALP expression decreased on days 3 and 7 (p < 0.05). Conclusions: These results suggest the MTA/BMP-2 combination promoted more rapid differentiation in MC3T3-E1 cells than did MTA/EMD during the early mineralization period.

• Asian-Australasian Journal of Animal Sciences
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• 제29권9호
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• pp.1229-1238
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• 2016

This study was conducted to determine the relationships of five intragenic single nucleotide polymorphism (SNP) markers (protein kinase adenosine monophosphate-activated ${\gamma}3$ subunit [PRKAG3], fatty acid synthase [FASN], calpastatin [CAST], high mobility group AT-hook 1 [HMGA1], and melanocortin-4 receptor [MC4R]) and meat quality traits of Duroc breeding stocks in Korea. A total of 200 purebred Duroc gilts from 8 sires and 40 dams at 4 pig breeding farms from 2010 to 2011 reaching market weight (110 kg) were slaughtered and their carcasses were chilled overnight. Longissimus dorsi muscles were removed from the carcass after 24 h of slaughter and used to determine pork properties including carcass weight, backfat thickness, moisture, intramuscular fat, $pH_{24h}$, shear force, redness, texture, and fatty acid composition. The PRKAG3, FASN, CAST, and MC4R gene SNPs were significantly associated with the meat quality traits (p<0.003). The meats of PRKAG3 (A 0.024/G 0.976) AA genotype had higher pH, redness and texture than those from PRKAG3 GG genotype. Meats of FASN (C 0.301/A 0.699) AA genotype had higher backfat thickness, texture, stearic acid, oleic acid and polyunsaturated fatty acid than FASN CC genotype. While the carcasses of CAST (A 0.373/G 0.627) AA genotype had thicker backfat, and lower shear force, palmitoleic acid and oleic acid content, they had higher stearic acid content than those from the CAST GG genotype. The MC4R (G 0.208/A 0.792) AA genotype were involved in increasing backfat thickness, carcass weight, moisture and saturated fatty acid content, and decreasing unsaturated fatty acid content in Duroc meat. These results indicated that the five SNP markers tested can be a help to select Duroc breed to improve carcass and meat quality properties in crossbred pigs.

• Ocean Science Journal
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• 제41권4호
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• pp.315-318
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• 2006

Three Synechococcus strains were isolated from seawater near the Ieodo Ocean Research Station (IORS), and their 16S rDNA genes and the internal transcribed spacer (ITS) between the 16S and 23S rRNA genes were sequenced to investigate their phylogenetic relationships. Phylogenetic trees based on the 16S rDNA and ITS sequences showed that they clustered in the main MC-A Synechococcus group (subcluster 5.1), but formed branches differentiating them from the described clades. As the IORS is located in an area affected by diverse water masses, high Synechococcus diversity is expected in the area. Therefore, the IORS might be a good site to study the diversity, physiology, and distribution of the Synechococcus group.

• Journal of Microbiology
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• 제38권2호
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• pp.80-87
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• 2000

The nucleotide sequence of the luxC gene coding for lux-specific fatty acyl-CoA reductase and the upstream DNA (325bp)of the structural gene from bioluminescent bacterium, Photobacterium phosphoreum, has been deternubed. An open reading frame extending for more than 20 codons in 325 bp DNA upstream of luxC was not present in both directions. The lux gene can be translated into a polypeptide of 54 kDa and the amino acid sequences of lux specific reductases of P. phosphoreum shares 80, 65, 58, and 62% identity with those of the Photobacterium leiognathi, Vibrio fischeri, Vibrio harveyi, and Xehnorhabdus luminescenens reductases, respectively. Analyses of codon usage, showing that a high frequency (2.3%) of the isoleucine codon, AUA, in the luxC gene compared to that found in Escherichia coli genes (0.2%) and its absence in the luxA and B genes, suggested that the AUA codon may play a modulator role in the expression of lux gene in E. coli. The structural genes (luxC, D, A, B, E) of the P. phosphoreum coding for luciferase (${\alpha}$,${\beta}$) and fatty acid reductase (r, s, t) polypeptides can be expressed exclusively in E. coli under the T7 phage RNA polymerase/promoter system and identificationof the [35S]methionine labelled polypeptide products. The degree of expression of lux genes in analyses of codon usage. High expression of the luxC gene could only be accomplished in a mutant E. coli 43R. Even in crude extracts, the acylated acyl-CoA reductase intermediate as well as acyl-CoA reductrase activities could be readily detected.

• 한국축산식품학회지
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• 제25권2호
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• pp.203-209
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• 2005

유전자수준의 염기서열에 근거를 둔 유전자 감식기법을 활용하여 개체간의 유전적 차이로 한우육과 젖소육 혹은 수입육의 구별방법을 개발하기 위한 연구가 많이 수행되었다. 그 중 real-time PCR 판별방법은 기존 PCR의 단점인 허위양성을 배제시켜 그 결과의 신뢰성이 높으며, PCR 산물들의 제한효소에 의한 절단 및 겔에서의 확인 등의 과정을 생략시킬 수 있어 짧은 시간 내에 다량의 시료를 분석할 수 있고 민감도가 높아 쇠고기가 포함된 가공식품의 분석도 가능하다는 장점이 있다. 본 연구는 시중에서 한우로 유통되는 쇠고기(생육 또는 양념육)의 DNA를 추출하여 real-time PCR을 통해서 모색유전자의 유전자 형(C-type, C/T-type or T-type)으로 한우육과 젖소육 및 수입육을 구분하였다. 한우육만을 판매한다는 시중 음식점 41개소에서 쇠고기(생육 또는 양념육)를 수거하여 분석한 결과, 분석된 41개의 시료 중 29개가 한우 유전자형으로, 12개의 시료가 젖소 또는 수입육 유전자형으로 판별되었다. 따라서 분석된 시료들의 한우육(T-type) 비율은 $70.1\%$, 그리고 젖소육 또는 수입육 비율은 $29.3\%$(C/T-type; $12.2\%$, C-type; $17.1\%$)이었다.