• Korean Journal of Animal Reproduction
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• v.22 no.3
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• pp.265-276
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• 1998

In an attempt to evaluate the function of MAP kinase of porcine oocytes and to develop a method of assessment for kinase activity, we used MBP as a substrate to detect the MAP kinase activity of porcine oocytes matured in in vitro. The MAP kinase which had lower activity during the first 20 hours of culture started to show an increased amount of activity at 25 hours at which a collapse in nuclear membrane was induced. Significant (P<0.05) a, pp.ared at 30 hours of being cultured. The gel phosphorylation method, MBP which has been known to be a substrate for kinase such as cdc2 kinase, was phosphorylated at two positions corresponding to ERK 1 (44kDa) and ERK2 (42 kDa) which are known as mammalian MAP kinase. The existence of MARKK and MAP kinase were identified with western blotting at 0 hour culture of immature GV oocytes. The amount of those proteins did not increase during 40 hours of culture, which suggest that the increase of MAP kinase activity was caused by phosphorylaton rather than due to change in protein amount. MAPKK and MAP kinase were shown to be dephosporylated with deactivated at M 1 stage by inhibition of protein synthesis with cycloheximide added at the strat following the cultrue. We have reulsts that indicate the existedence of MAP kinase cascade which was activated simultaneously with start of porcine oocyte maturation (GVBD).

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2012.07a
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• pp.134-137
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• 2012

최근 ATSC방식의 디지털 방송에서 HD급의 3D 방송 서비스에 대한 관심이 증가 되고 있는 추세이다. HD급의 3D 방송 서비스(이하 3D HDTV)를 제공하기 위해 [2]에서는 ATSC 전송 시스템을 확장하고 BCH 부호와 IRA 타입의 LDPC를 사용하는 수정된 ATSC 전송 시스템을 제안하였다. 본 논문에서는 BCH와 IRA 타입의 LDPC 부호 대신 Reed Solomon 부호와 Block LDPC 부호를 사용하고 16QAM 변조를 사용하여 전송 용량을 증대할 수 있다는 사실을 확인하였다. AWGN 채널에서 모의 실험한 결과 BCH부호와 LDPC부호 및 4PAM을 적용한 수정된 ATSC 전송시스템의 TOV는 약 7dB로 19.33Mbps로 전송되지만[2] 본 논문에서 제안한 RS(207, 187)과 부호율 3/4인 Block LDPC 및 16QAM의 TOV는 약 5.4dB로 29Mbps로 전송되어 기존시스템에 비해 약1.5배의 전송 용량 증대가 가능한 것으로 확인되었다. 이로서 6MHz의 한정된 대역에서 HD급의 3D 방송서비스 실현이 한층 더 가까워지게 될 것으로 예상된다.

• Journal of Digital Contents Society
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• v.5 no.4
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• pp.306-310
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• 2004

In this paper, we proposed a capacity enhancement method using TCM-128QAM modulation and bandwidth extension of 25MHz in HDR-WPAN (high data rate wireless personal area network) system. In case using TCM-128 QAM modulation scheme, we can obtain maximum 66Mbps transmission rate in bandwidth of 15MHz. If extend bandwidth by 25MHz and roll-off coefficient ${\alpha}=0.35$, we can obtain maximum 110Mbps transmission rate. These results are confirmed through PSD(power spectrum density) analysis and BER (bit error rate) performance.

• Molecules and Cells
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• v.25 no.3
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• pp.446-451
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• 2008

We assessed heterologous protein expression in 64 strains obtained from the Escherichia coli Reference (ECOR) collection, a collection representing diverse natural E. coli populations. A plasmid generating a glutathione S-transferase and plant carbonic anhydrase fusion protein (GST-CA) under the control of the tac promoter was introduced into the ECOR strains, and the quantity of the fusion protein was determined by SDS-PAGE. The foreign protein was generated at various levels, from very high (40 strains, high producers) to very low (six strains, low producers). Immunoblotting showed that the high producers expressed approximately 250-500 times more GST-CA protein than the low producers. The results of semi-quantitative RT-PCR showed that the low producers generated mRNA levels comparable to those of the high producers, thereby suggesting that, at least in this case, inefficient translation is a major cause of the low production. We introduced a different plasmid, which expressed a maltose binding protein and plant guanylate kinase fusion protein (MBP-GK) into the six low producers. Interestingly, five of these expressed MBP-GK at very high levels. Thus, we conclude that the production of a particular protein from an expression vector can vary considerably, depending on the host strain. Strains in the ECOR collection could function as useful alternative hosts when a desired level of protein expression is not obtained from commonly used strains, such as E. coli K12 or B derivatives.

• Electronics and Telecommunications Trends
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• v.23 no.1 s.109
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• pp.99-108
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• 2008

3G LTE 이동통신 시스템은 패킷 데이터 전송에 기반을 둔 다양한 서비스 지원을 목표로 하는 기술로서 최대 20MHz 대역폭 기준 하향링크 최대 전송속도 100Mbps, 상향 링크 50Mbps의 전송속도를 지원한다. 그리고 데이터 전송 효율 향상, 효율적인 주파수 자원 이용, 이동성, 낮은 latency, 패킷 데이터 전송에 최적화된 기술과 서비스 품질보장 등을 제공한다. 3G LTE 시스템은 기존 시스템에 비해 주파수 및 고속의 멀티미디어 서비스를 효율적으로 사용하는 IP 네트워크로 진화되는 이동통신 시스템이다. 3G LTE 이동통신 단말은 대역폭 20MHz 기준으로 이동속도 120km/h에서 하향링크 30 Mbps, 상향링크 15Mbps의 데이터 전송속도를 지원한다. 또한, 고품질 및 고속의 멀티미디어 서비스를 제공하는 단말로서 3.5세대인 HSDPA에 반해 모바일 영상 서비스가 본격적으로 제공되는 3G LTE 이동통신 시스템의 단말이다. 본 고에서는 3G LTE 이동통신 시스템 단말 플랫폼 기술 동향과 전망에 대하여 논의한다. II장에서는 3G LTE 표준 규격을 기반으로 구현하는 3G LTE 이동통신 시스템 및 단말 플랫폼을 서술하며, III장에서는 국ㆍ내외 단말 플랫폼 기술 동향에 관한 내용을 기술한다. IV장에서는 향후 발전 전망에 대해 살펴보고, 마지막으로 결론을 맺고자 한다.

• Korean Journal of Microbiology
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• v.55 no.3
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• pp.283-285
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• 2019

The draft genome sequencing for Pelagicola sp. DSW4-44 (= KCTC 62762 = KCCM 43261), isolated from deep seawater of East Sea in Korea, was performed using Illumina HiSeq platform. As a result, the draft genome was comprised of a total length of approximately 4.85 Mbp with G + C content of 54.3%, and included a total of 4,566 protein-coding genes, 3 rRNA genes, 48 tRNA genes, 3 non-coding RNA genes, and 67 pseudo genes. In the draft genome, the strain DSW4-44 contained genes involved in the nitrogen metabolism of dissimilatory nitrate reduction to ammonium (DNRA) and denitrification, which were not found other strains in the genus Pelagicola.

• Korean Journal of Microbiology
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• v.54 no.4
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• pp.480-482
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• 2018

The draft genome sequencing for Zhongshania marina $DSW25-10^T$, isolated from deep seawater of East Sea in Korea, was performed using Illumina HiSeq platform. As a result, the draft genome was comprised of a total length of approximately 4.08 Mbp with G + C content of 49.0%, and included a total of 3,702 protein-coding genes, 3 rRNA genes, 39 tRNA genes, 4 non-coding RNA genes, and 36 pseudogenes. In addition, the metabolic pathways of aliphatic and aromatic compounds were identified. In light of these metabolic pathways, Zhongshania marina $DSW25-10^T$ is expected to be a useful bioremediation resource.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.4
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• pp.448-457
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• 2015

Developing rice lines with various amylose contents is necessary to diverse usages of rice in terms of raw materials for processed food production, and thereby to promote rice consumption in Korea. A rice mutant line, 'Namil(SA)-dull1' was established through sodium azide mutagenesis on 'Namil', a non-glutinous Korean Japonica rice cultivar. Namil(SA)-dull1' had dull endosperm characteristics and the evaluated amylose content was 12.2%. A total of 94 F2 progenies from a cross between 'Namil(SA)-dull1' and 'Milyang23', a non-glutinous Tongil-type rice cultivar, was used for genetic studies on the endosperm amylose content. Association analyses, between marker genotypes of 53 SSR anchor markers and evaluated amylose contents of each 94 F2:3 seeds, initially localized rice chromosome 6 as the harboring place for the modified allele(s) directing low amylose content of 'Namil(SA)-dull1'. By increasing SSR marker density on the putative chromosomal region followed by association analyses, the target region was narrowed down 0.94 Mbp segment, expanding from 28.95 Mbp to 29.89 Mbp, on rice chromosome 6 pseudomolecule. Among the SSR loci, RM7555 explained 84.2% of total variation of amylose contents in the $F_2$ population. Further physical mapping on the target region directing low amylose content of 'Namil(SA)-dull1' would increase the breeding efficiency in developing promising rice cultivars with various endosperm characteristics.

• Journal of Life Science
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• v.19 no.2
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• pp.284-288
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• 2009

The innate immune system is important for the first line of host defence against infectious agents, which have penetrated the mechanical barriers. Mannose-binding lectin (MBL or mannan-binding protein, MBP) is a serum protein that is synthesized in the liver as a part of the acute phase response. MBL binds to carbohydrate structures presented by a wide range of pathogenic bacteria, viruses, fungi, and parasites. MBL is synthesized as a monomer that has a carboxy-terminal carbohydrate recognition domain, a neck region and a collagen region. Low MBL level was reported to be the most frequent immuno-deficiency syndrome. Although extensive studies have yielded detailed information on the structure of MBL, functions of the MBL complex are not fully understood yet. We, here, present cloning process of MBL cDNA from the rat liver and production of truncated recombinant MBL protein using a bacterial expression system in order to produce anti-MBL polyclonal antibody. Anti-MBL polyclonal antibody was raised in a New Zealand rabbit and its affinity was tested against recombinant protein using western blot technique. MBL cDNA, recombinant protein and anti-MBL antibody could be used as great arsenals to dissect cellular biochemistry of MBL.

• Journal of Microbiology and Biotechnology
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• v.31 no.11
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• pp.1583-1590
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• 2021

Studies have demonstrated that PE_PGRS45 is constitutively expressed under various environmental conditions (such as nutrient depletion, hypoxia, and low pH) of the in vitro growth conditions examined, indicating that PE_PGRS45 protein is critical to the basic functions of Mycobacterium tuberculosis. However, there are few reports about the biochemical function and pathogenic mechanism of PE_PGRS45 protein. The fact that this M. tuberculosis gene is not easily expressed in E. coli may be mainly due to the high content of G+C and the use of unique codons. Fusion tags are indispensable tools used to improve the soluble expression of recombinant proteins and accelerate the characterization of protein structure and function. In the present study, His6, Trx, and His6-MBP were used as fusion tags, but only MBP-PE_PGRS45 was expressed solubly. The purification using His6-MBP tag-specific binding to the Ni column was easy to separate after the tag cleavage. We used the purified PE_PGRS45 to immunize New Zealand rabbits and obtained anti-PE_PGRS45 serum. We found that the titer of polyclonal antibodies against PE_PGR45 was higher than 1:256000. The result shows that purified PE_PGRS45 can induce New Zealand rabbits to produce high-titer antibodies. In conclusion, the recombinant protein PE_PGRS45 was successfully expressed in E. coli and specific antiserum was prepared, which will be followed by further evaluation of these specific antigens to develop highly sensitive and specific diagnostic tests for tuberculosis.