• Journal of Microbiology and Biotechnology
• /
• v.10 no.2
• /
• pp.181-186
• /
• 2000

MAPI (microbial alkaline protease inhibitor) was isolated from cultrue broth of Streptomyces chromofuscus SMF28. The Ki values of MAPI for the representative serine proteases such as chymotrypsin and proteinase K were 0.28 and $0.63{\;}\mu\textrm{M}$, respectively, and for the cysteine proteases cathepsin B and papain were 0.66 and $0.28{\;}\mu\textrm{M}$, respectively. These data indicate that MAPI is not a potent selective inhibitor of serine or cysteine proteases. Progress curves for the inhibition of three proteases by MAPI exhibithe characteristic patterns; MAPI exhibited slow-binding inhibition of cathepsin B. It was rapidly associated with chymotrypsin before the addition of substrate and then reactivation of MAPI-inhibited enzyme was investigated in the presence of substrate. On the other hand, MAPI-proteinase K interaction was typical for those classical inhibitors. When MAPI was incubated with trypsin, there was an extensive reduction in the ingibitory activities of MAPI corresponding to 66.5% inactivation of MAPI, indicating that trypsin-like protease may play a role in the decrease of the inhibitory activity during cultivation.

• Journal of Sensor Science and Technology
• /
• v.33 no.2
• /
• pp.93-97
• /
• 2024

With the development of promising future mobility and urban air mobility (UAM) technologies, the demand for LIDAR sensors has increased. The SWIR photodetector is a sensor that detects lasers for the 3D mapping of lidar sensor and is the most important technology of LIDAR sensor. An SWIR photodetector based on QDs in an eye-safe wavelength band of over 1400 nm has been reported. QDs-based SWIR photodetectors can be synthesized and processed through a solution process and have the advantages of low cost and simple processing. However, the organic ligands of QDs have insulating properties that limit their ability to improve the sensitivity and stability of photodetectors. Therefore, the technology to replace organic ligands with inorganic ligands must be developed. In this study, the organic ligand of the synthesized PbS QDs was replaced with a MAPI inorganic ligand, and an SWIR photodetector was fabricated. The analysis of the characteristics of the manufactured photodetector confirmed that the photodetector based on MAPI-capped PbS QDs exhibited up to 26.5% higher responsivity than that based on organic ligand PbS QDs.

• Journal of the Korean Society of Industry Convergence
• /
• v.6 no.3
• /
• pp.223-230
• /
• 2003

Generally, the life-span of a multi-housing complex is over 50 years, but in reality they are usually demolished after 20 years in spite of its remaining life expectancy. Thus, this research focuses on the estimation of the optimum economic life-span of a multi-housing complex. To estimate the minimum total cost point of start to finish of a multi-housing complex, we'll apply MAPI(Machinery and Allied Product Institute) and LCC(Life Cycle Cost) theory.

• Journal of Korean Institute of Industrial Engineers
• /
• v.3 no.2
• /
• pp.57-62
• /
• 1977

We consider the problem of determining the optimal replacement policy of the items which deteriorate appreciably with time and become obsolete. Most of the conventional engineering economy models including the widely-accepted MAPI model assume linearity of technological improvement over time, in obtaining the economic lives of items and the net present values of the replacement alternatives under consideration. The main achievement of this paper is in that it successfully relaxes this strict linearity assumption to accommodate various other types of technological improvements in determining those values. Based on these results, we also include in this paper the mathematical models by which to determine the optimal replacement policies of items both under and without capital budgeting constraints.

• The Korean Journal of Pain
• /
• v.30 no.3
• /
• pp.197-206
• /
• 2017

Background: Pain with neuropathic characteristics is generally more severe and associated with a lower quality of life compared to nociceptive pain (NcP). Short form of the Douleur Neuropathique en 4 Questions (S-DN4) is one of the most used and reliable screening questionnaires and is reported to have good diagnostic properties. This study was aimed to cross-culturally validate the Hindi version of the S-DN4 in patients with various chronic pain conditions. Methods: The S-DN4 is already translated into the Hindi language by Mapi Research Trust. This study assessed the psychometric properties of the Hindi version of the S-DN4 including internal consistency and test-retest reliability after 3 days' post-baseline assessment. Diagnostic performance was also assessed. Results: One hundred sixty patients with chronic pain, 80 each in the neuropathic pain (NeP) present and NeP absent groups, were recruited. Patients with NeP present reported significantly higher S-DN4 scores in comparison to patients in the NeP absent group (mean (SD), 4.7 (1.7) vs. 1.8 (1.6), P < 0.01). The S-DN4 was found to have an AUC of 0.88 with adequate internal consistency (Cronbach's ${\alpha}=0.80$) and a test-retest reliability (ICC = 0.92) with an optimal cut-off value of 3 (Youden's index = 0.66, sensitivity and specificity of 88.7% and 77.5%). The diagnostic concordance rate between clinician diagnosis and the S-DN4 questionnaire was 83.1% (kappa = 0.66). Conclusions: Overall, the Hindi version of the S-DN4 has good internal consistency and test-retest reliability along with good diagnostic accuracy.