• Title/Summary/Keyword: M50

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Enzymological Evaluation of Oral Inflammation inhibitory activity by Aloe vera peel extract (Aloe vera peel 추출물에 의한 구강염증 저해 효과의 효소학적 평가)

  • Pack, Chung-Sun;Ryu, Il-Hwan;Lee, Kap-Sang
    • Korean Journal of Food Science and Technology
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    • v.33 no.6
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    • pp.753-759
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    • 2001
  • To evaluate antiinflammation of Aloe vera peel, antiimflammation substances were extracted from Aloe vera peel and identified, and we investigated the effect of the its substance the inhibitory effect on the activity of hyaluoronidase, elastase, collagenase and prostaglandin endoperoxide synthase. The water extract from Aloe vera peel were successfully purified with solvent fractionation, silica gel column chromatography, preparative thin layer chromatography and UV spectrometer. Two purified active substances were identified as aloe-emodin and barbaloin by Mass Spectrometer, $^1H-NMR$ and FT-IR. Aloe-emodin and barbaloin. $IC_{50}$ values of aloe-emodin and barbaloin against hyaluronidase activity were 40 and $70\;{\mu}g/mL$, respectively. Leuckocyte elastase, which is related to the destruction of various tissue, $IC_{50}$ values of them were 50 and $60\;{\mu}g/mL$, respectively. $IC_{50}$ values of aloe-emodin and barbaloin against collagenase activity were 40 and $60\;{\mu}g/mL$, respectively. and $IC_{50}$ values of aloe-emodin and barbaloin aganist the prostaglandin endoperoxide synthase, which play an important role in inflammatory reactions, were 40 and $70\;{\mu}g/mL$, respectively. Inhibitory effects of aloe-emodin, barbaloin and aspirin against carrageenan paw edema were 74.9, 52.9 and 51.9% as inhibiton percentage, respectively, at dose of 100 mg/kg and that of indomethancin was 49.7 at dose of 10 mg/kg. Cell cytotoxicity of barbaloin against human gingival cells was lower than that of aloe-emodin. Aloe-emodin and barbaloin did not show cytotoxicity against human gingival cells at concentration of 1.0 and $5.0\;{\mu}g/mL$, However, aloe-emodin and barbaloin showed less cytotoxicity than chlorhexidine, which usually have been used as the agent of anticaries and antiinflammation. These results suggested that aloe-emodin and barbaloin from Aloe vera peel have the effect of anticaries and antiinflammation.

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Antioxidative Activities and Antiaging Effects of Geum aleppicum Jacq. Extracts (큰뱀무 추출물의 항산화 활성 및 항노화에 관한 연구)

  • Kim, Min-Ji;Yang, Hyun-Gab;Park, Soo-Nam
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.37 no.2
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    • pp.191-198
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    • 2011
  • In this study, antioxidative effects and inhibitory effects of Geum aleppicum Jacq. extracts on tyrosinase and elastase were investigated. The ethyl acetate fraction of G. aleppicum Jacq. extract ($4.70\;{\mu}g$/mL) showed the most prominent free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC50). Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of some G. aleppicum Jacq. extracts on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system were investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction showed the most prominent ROS scavenging activity ($0.22 \;{\mu}g$/mL). The protective effects of extract/fraction of G. aleppicum Jacq. against the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The G. aleppicum Jacq. extracts suppressed photohemolysis in a concentration dependent manner ($1{\sim}25{\mu}g$/mL), particularly the ethyl acetate fraction exhibited the most prominent celluar protective effect (${\tau}_{50}$, 416.20 min at $10 \;{\mu}g$/mL). The inhibitory effect of G. aleppicum Jacq. extracts on tyrosinase and elastase were investigated to assess their whitening and anti-winkle efficacy. The half maximal inhibitory concentration ($IC_{50}$) of the ethyl acetate fraction on tyrosinase was $95.23\;{\mu}g$/mL. The $IC_{50}$ of 50 % ethanol extract and the ethyl acetate fraction on elastase were $6.27 \;{\mu}g$/mL and $4.31 \;{\mu}g$/mL, respectively. These results indicate that extract/fraction of G. aleppicum Jacq. can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against ROS. Especially the ethyl acetate fraction of G. aleppicum Jacq. extracts could be applicable to new functional cosmetics for antioxidant, antiaging.

Dose Reduction According to the Exposure Condition in Intervention Procedure : Focus on the Change of Dose Area and Image Quality (인터벤션 시 방사선조사 조건에 따른 선량감소 : 면적선량과 영상화질 변화를 중심으로)

  • Hwang, Jun-Ho;Jung, Ku-Min;Kim, Hyun-Soo;Kang, Byung-Sam;Lee, Kyung-Bae
    • Journal of radiological science and technology
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    • v.40 no.3
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    • pp.393-400
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    • 2017
  • The purpose of this study is to suggest a method to reduce the dose by Analyzing the dose area product (DAP) and image quality according to the change of tube current using NEMA Phantom. The spatial resolution and low contrast resolution were used as evaluation criteria in addition to signal to noise ratio (SNR) and contrast to noise ratio (CNR), which are important image quality parameters of intervention. Tube voltage was fixed at 80 kVp and the amount of tube current was changed to 20, 30, 40, and 50 mAs, and the dose area product and image quality were compared and analyzed. As a result, the dose area product increased from $1066mGycm^2$ to $6160mGycm^2$ to 6 times as the condition increased, while the spatial resolution and low contrast resolution were higher than 20 mAs and 30 mAs, Spatial resolution and low contrast resolution were observed below the evaluation criteria. In addition, the SNR and CNR increased up to 30 mAs, slightly increased at 40 mAs, but not significantly different from the previous one, and decreased at 50 mAs. As a result, the exposure dose significantly increased due to overexposure of the test conditions and the image quality deteriorated in all areas of spatial resolution, low contrast resolution, SNR and CNR.

Physiological Response of Chinese Cabbage to Salt Stress (염 스트레스에 대한 배추의 생리학적 반응)

  • Kim, Ju-Sung;Shim, Ie-Sung;Kim, Myong-Jo
    • Horticultural Science & Technology
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    • v.28 no.3
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    • pp.343-352
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    • 2010
  • In order to understand the plant responses to salt stress (0, 50, and 100 mM NaCl), Chinese cabbage seedlings grown up to two leaf stages by hydroponic culture were used. Fresh and dry weight, chlorophyll (Chl), antioxidant materials, polyamine content, antioxidant enzyme activities, and inorganic ion level were evaluated. Fresh and dry weights of Chinese cabbage increased with the increase in salinity while the optimal growth occurred at 50 mM NaCl. The Chl a, total Chl, carotenoid content, and Chl a/b ratio increased by the 6 days after treatment with 100 mM NaCI; however, the Chl b content decreased. Glutathione increased in the root of Chinese cabbage for 6 days. Dehydroascorbate increased remarkably by day 6 caused by the salt stress in both leaf and the root. While ascorbate peroxidase increased, the activity of catalase and glutathione reductase decreased gradually in the first leaf for 6 days. The $Na^+$ content increased by 12.5-fold in the 3 days after treatment with 100 mM NaCI in the shoot, whereas the $Ca^{2+}$, $K^+$, and $Mg^{2+}$ content measured in the same treatment decreased by 43 to 57%. Spermidine content decreased as salinity increased, but spermine content increased. The growth promotion, glutathione and ascorbic acid content in Chinese cabbage were increased by low salt stress, and shortening of the cultivation period for growth increase of Chinese cabbage is expected.

Change of Ion Concentrations in Soil Solution According to Different Cutting Intensities and Sampling Times in Pinus rigida Plantations (리기다소나무조림지(造林地)의 벌채수준(伐採水準) 및 시료(試料) 채취시기(採取時期)에 따른 토양수내(土壤水內) 이온농도(濃度) 변화(變化))

  • Oh, Kwang-In;Cho, Hi-Doo;An, Ki-Wan;Kim, Choonsig
    • Journal of Korean Society of Forest Science
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    • v.88 no.4
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    • pp.438-445
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    • 1999
  • The concentrations of some ions ($NO{_3}^-$, $Ca^{2+}$, $Mg^{2+}$, $NH{_4}^+$) in soil solution collected by zero tension lysimeter in mature Pinus rigida plantations in Goksung, Jeonranam-do were measured at two soil depths (10cm and 30cm) following various levels of cutting intensity treatment (uncut, $6m{\times}50m$, $10m{\times}50m$, $20m{\times}50m$ strip crearcutting) three times (July 6. July 30, and August 4) between June 20 and August 4 1998. The ion concentrations in the soil solution were significantly different among sampling times, while the concentrations were not different among cutting levels or sampling depths. The ion concentrations in the soil solution decreased in the order of $NO{_3}^-$ > $Ca^{2+}$ > $Mg^{2+}$ > $NH{_4}^+$ and the mean concentration was 3.60mg/L for $NO{_3}^-$, 1.7mg/L for $Ca^{2+}$, 0.5mg/L for $Mg^{2+}$, and 0.04mg/L for $NH{_4}^+$ respectively. These ion concentrations except for $NH{_4}^+$ ion were negatively correlated with the volume of collected soil solutions (r=-0.31~-0.41). The results suggest that the change of nutrient concentrations in the soil solution collected from the P. rigida plantations was related to the temporal input patterns of precipitation rather than the cutting intensity.

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Effects of Organic Binder and Film Thickness on Optoelectrical Properties of AC Powder EL Devices Prepared by Screen Printing Method for LCD Backlight Applications (LCD 백라이트를 위해 스크린 프린팅법으로 제조된 AC Powder EL 소자의 유기결합제와 막두께가 광전기적 성질에 미치는 영향)

  • Lee, Kang-Ryeol;Park, Sung
    • Journal of the Korean Ceramic Society
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    • v.38 no.12
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    • pp.1085-1092
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    • 2001
  • The high efficient AC powder EL devices classified by low cost and low power consumption type fabricated using screen printing method with film thickness and organic binder. Brightness and current density were measured at frequency range of 400Hz∼1kHz and voltage range of 50∼300V$\_$rms/ to estimate optoelectrical properties of AC powder EL devices, respectively. Frequency generator was used as system producing frequency and voltage of a sine wave. Also brightness and current density were measured by luminometer and multimeter. In the case of low cost type AC powder EL device, brightness and current density were about 43 cd/m$^2$and 20$\mu$A/cm$^2$when the thickness of phosphor and dielectric layer was 45∼50$\mu$m under no addition of plasticizer respectively. In the case of low power consumption type AC powder EL device, brightness and current density were about 74 cd/m$^2$and 30∼40$\mu$A/cm$^2$when the thickness of phosphor and dielectric layer was 45∼50$\mu$m and 15∼20$\mu$m under addition of 15wt% plasticizer respectively. Also, AC powder EL device fabricated in this study showed absolutely excellent characteristics as the lifetime was longer than products of other company.

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Infill-system Development for Long-Life Housing with Durability & Flexibility: Focused on the Constructing and Evaluating Application of Mock-up House (내구성 및 가변성을 갖는 장수명 공동주택의 가변요소 시스템 개발 : 실험주택 건립 및 적용성 평가를 중심으로)

  • Cheong, So-Yi;Park, Joon-Young;Chun, Young-Soo
    • Land and Housing Review
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    • v.2 no.4
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    • pp.397-406
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    • 2011
  • This research aims to develop various infill-systems for practically applying to long-life housing with 100-year durability and flexibility as a strategy for realizing sustainable residential environment. Especially, the developed infill-systems & technologies were concentrated to the mock-up house applied with the standard model of long-life housing, and their in-situ applicability by a phase, experts' consultation, and questionnaire survey were also evaluated. This research was executed to divide by 3 phases. In the First phase infill-systems for a housing unit of $84m^2$ exclusive use area as well as a support of whole structure and infill-systems including dry wall, and windows were constructed, and their in-situ applicability were evaluated. In the Second phase vertical integration of upper and lower housing units of $50m^2$ and $40m^2$ exclusive use area was investigated the first in domestic for 3 housing units of $50m^2$ exclusive use area. In addition, various infill-systems including the horizontal expansion of $50m^2$ and $10m^2$ exclusive use areas were also constructed and evaluated on their in-situ applicability. In the third phase diverse performance tests and field investigation for in-situ verifying of dry Ondol, developed this research, were executed to improve their practicability. Inner dry walls were taken apart, moved and re-constructed for verifying their practicability to investigate 3R-Reduce, Reuse and Recycle-realization too.

오염지하수정화를 위한 반응벽체의 설계 및 시공

  • 이동호;지원현;이재원;박준범
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.04a
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    • pp.434-437
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    • 2004
  • 본 반층벽체 적용지역의 경우, 침출수의 영향으로 암모니아성 질소의 농도가 최대 29.12mg/L까지 검출되고 있으며 반응물질은 암모니아성 질소제거에 적합한 제올라이트를 선정하였다. 투수성 및 다짐강도를 고려하여 왕사와 50:50의 비율로 혼합, 적용하였으며 지하수 평균유속은 0.0864m/d이고 평균지하수위는 원지반 기준 - 4.0m의 분포를 나타내었다. 지질조사 결과, 적용구간의 지질구조는 매립층, 풍화토, 풍차암, 연암의 순서로 분포하고 있으며, 반응벽체의 설계깊이는 현장 시추조사 결과를 바탕으로 지하수의 Under-pass를 방지하기 위하여 연암층 50cm까지 관입시키도록 설계하였다. 최종적으로 선정된 반응벽체의 규모는 35m(길이) $\times$ 1.2m(두께) $\times$ 8.5 ~ 9.42m(구간별 깊이)로 설계에 반영되었다. 현재, 지하수감시정을 이용한 모니터링 결과, 상부지하수 감시정에서의 오염원은 우기의 영향으로 간헐적으로 발생하고 있으나 하부 지하수 감시정에서의 암모니아성 질소농도는 불검출 되거나 0.5 mg/L 이하로 유지되고 있다.

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Antioxidative Properties of Different Solvent Extracts from Persimmon (Diospyros kaki cv. Fuyu) Flower-Buds

  • You, Dong-Hyun;Lee, Seung-Cheol
    • Preventive Nutrition and Food Science
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    • v.16 no.4
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    • pp.328-332
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    • 2011
  • After preparation of acetone, ethanol, methanol, and water extracts (10 g/300 mL) of dried persimmon (Diospyros kaki cv. Fuyu) flower-buds, total phenolic contents (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (RSA), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) RSA, reducing power (RP), and tyrosinase inhibitory activity of the extracts were evaluated. The methanol extracts produced the highest TPC (113.39 mg gallic acid equivalents/g), DPPH RSA ($IC_{50}=40.25\;{\mu}g/mL$), ABTS RSA ($IC_{50}=58.17\;{\mu}g/mL$) and RP ($IC_{50}=69.43\;{\mu}g/mL$) activities while the water extracts generated the lowest values. The ethanol extract showed the highest tyrosinase inhibitor activity (88.90%) at a concentration of 1 mg/mL. These results indicated that persimmon flower-buds may be a useful source of natural antioxidants.

Design of a Focusing Optical System (집속광학계의 설계)

  • 이동희
    • Proceedings of the Optical Society of Korea Conference
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    • 2000.02a
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    • pp.220-221
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    • 2000
  • 본 연구에서 목표로 하는 집속광학계는 레이저 다이오드의 emitting area가 50$mu extrm{m}$$\times$1.0$\mu\textrm{m}$ 이고 pumping 매질인 crystal의 TE $M_{00}$ mode 발생을 위한 최소 입사빔의 단면도가 단축과 장축의 직경이 각각 206$\mu\textrm{m}$, 204$\mu\textrm{m}$인 타원이어야 하기 때문에 수직 방향으로는 200배 수평 방향으로는 4배의 확대를 할 수 있는 광학계 이어야 한다. 일반적으로 레이저 다이오드용 집속광학계는 레이저다이오드의 수직 수평 emitting area가 다르기 때문에 실린더형, 원통형 또는 toroidal형의 렌즈를 조합하여 구성한다. 그러나 우리의 경우는 emitting area의 수직 수평비 즉 집속광학계의 수직 수평의 굴절능( power )의 비가 50:1 이어야 하기 때문에 그림1과 같은 일반적인 실린더형, 원통형 또는 toroidal형의 렌즈의 조합으로 집속광학계를 구성하기에는, 렌즈 power비를 50:1로 하기 위해 필요로 하는 공간이( 첫번 렌즈와 마지막 렌즈까지의 거리 ) 커지는 점, 필요로 하는 렌즈의 매수가 많아지는 점, 집속 beam의 형상 즉 단면의 이심률이 커져서 pumping에 비효율적이라는 점 등에서, 부적절하다. (중략)략)

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