• ALGAE
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• 제32권2호
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• pp.139-153
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• 2017

The ability of a red tide species to take up nutrients is a critical factor affecting its red tide dynamics and species competition. Nutrient uptake by red tide species has been conventionally measured by incubating nutrient-depleted cells for a short period at 1 or 2 light intensities. This method may be applicable to certain conditions under which cells remain in oligotrophic water for a long time and high nutrients are suddenly introduced. Thus, a new method should be developed that can be applicable to the conditions under which cells are maintained in eutrophicated waters in healthy conditions and experience light and dark cycles and different light intensities during vertical migration. In this study, a new repletion method reflecting these conditions was developed. The nitrate uptake rates of the red tide dinoflagellate Prorocentrum micans originally maintained in nitrate repletion and depletion conditions as a function of nitrate concentration were measured. With increasing light intensity from 10 to $100{\mu}E\;m^{-2}s^{-1}$, the maximum nitrate uptake rate ($V_{max}$) of P. micans increased from 3.6 to $10.8 pM\;cell^{-1}d^{-1}$ and the half saturation constant ($K_{s-NO3}$) increased from 4.1 to $6.9{\mu}M$. At $20{\mu}E\;m^{-2}s^{-1}$, the $V_{max}$ and $K_{s-NO3}$ of P. micans originally maintained in a nitrate repletion condition were similar to those maintained in a nitrate depletion condition. Thus, differences in cells under nutrient repletion and depletion conditions may not affect $K_{s-NO3}$ and $V_{max}$. Moreover, different light intensities may cause differences in the nitrate uptake of migratory phototrophic dinoflagellates.

• 한국운동역학회지
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• 제23권2호
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• pp.99-108
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• 2013

The purpose of this study was to analyze the kinematic characteristics of javelin to compare the characteristics between world elite athletes and local male athletes. The subjects selected 9 athletes out of total 13 athletes recorded more than 65 m in the preliminary and main competition participated in the 93rd National Sports Festival held in 2012. Three-Dimensional motion analysis using a system of 4 video cameras(Sony HXR-MC2000) at a sampling frequency of 60 fields/s was performed for this study. The Kwon3D 3.1 was used to obtain the three dimensional coordinates about the top, grip, end of javelin. And the kinematic factors of javelin calculated using Matlab2009a program. The resultant speed of javelin that affects directly to the record of performance showed 26.08 m/s indicated lower speed of about 2-3 m/s than world elite athletes. The release point appeared to have been made at the high of $1.79{\pm}0.07$ m of 99.8% of the height of the athletes. In terms of release angle, it was indicated average $33.0{\pm}3.81^{\circ}$ lower release angle compared to the world elite athletes. The attitude angle(up & down tilt angle, X axis) related to javelin indicated average $38.5{\pm}4.96^{\circ}$, its related attack angle average $5.5{\pm}5.11^{\circ}$, and yaw angle(sideslip, side attack angle) average $15.7{\pm}8.48^{\circ}$.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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• pp.290-290
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• 1994

The binding characteristic of oxomemazine to muscarinic receptor in the cerebrum, heart, and ileum were compared to those of pirenzepine to investigate whether oxomemazine could classify the muscarinic receptor subtypes. 〔$^3$H〕Quinucl idinyl benzilate(QNB) identified a single class of muscarinic receptors with apparent K$\sub$D/ value of about 60 pM in three tissues. Analysis of the pirenzepine inhibition curve of 〔$^3$H〕QNB binding to cerebral microsome indicated the presence of two receptor subtypes with high (Ki=16 nM, M$_1$-receptor) and low (Ki=400 nM, M$_2$-receptor) affinity for pirenzepine. Oxomemazine also identified two receptor subtypes with high (Ki=84 nM, On-receptor) and low (Ki=1 4 ${\mu}$M, O$\sub$L/-receptor) affinity in rat cerebral microsome, The percentage population of the M$_1$-and M$_2$-receptors to the total receptors were 61 : 39, and those of the O$\^$H/- and O$\sub$L/-receptors 39 : 61, respectively, However, the Hill coefficients of these two drugs for the inhibition of 〔$^3$H〕QNB binding to the heart and ileum were close to unity which indicated that these drugs bound to a uniform population of receptors in these two tissues. The Ki values for the low affinity sites of pirenzepine and oxomemazine in the cerebrum were similar to those of these drugs in the heart ileum. Both pirenzepine and oxomemazine increased K$\sub$D/ value for 〔$^3$H〕QNB without affecting the binding sites concentration and Hill coefficient for the 〔$^3$H〕QNB binding. Oxomemazine had a 10-fold lower affinity at Ma-receptors than at M$_1$-receptors, and pirenzepine a 8-fold lower affinity at O$\sub$L/-receptors than OH-receptors. Analysis of the shal low competition curves of oxomemazine for the H$_1$ receptors and pirenzepine for the O$\sub$L/-receptors yielded that 69% of the M$_1$-receptors were of the O$\sub$H/-receptors and the remaining 31% of the O$\sub$L/-receptors, and that 29% of the O$\sub$L/-receptors were of the M$_1$-receptors and 71% of the M$_2$-receptors. However, M$_2$ for oxomemazine and O$\sub$H/ for pirenzepine were composed of a uniform population. These results suggest that oxomemazine could discriminatethe muscarnic receptor subtypes and may subclassify the M$_1$-receptors into two subtypes.

• BMB Reports
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• 제36권3호
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• pp.305-311
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• 2003

A modified actinomycin D was prepared with a hydroxyl group that replaced the amino group at the chromophore 2-position, a substitution known to strongly reduce affinity for double-stranded DNA. Interactions of the modified drug on single-stranded DNAs of the defined sequence were investigated. Competition assays showed that 2-hydroxyactinomycin D has low affinity for two oligonucleotides that have high affinities ($K_a\;=\;5-10{\times}10^6\;M^{-1}$ oligomer) for 7-aminoactinomycin D and actinomycin D. Primer extension inhibition assays performed on several single-stranded DNA templates totaling around 1000 nt in length detected a single high affinity site for 2-hydroxyactinomycin D, while many high affinity binding sites of unmodified actinomycin D were found on the same templates. The sequence selectivity of 2-hydroxyactinomycin D binding is unusually high and approximates the selectivity of restriction endonucleases. Binding appears to require a complex structure, including residues well removed from the polymerase pause site.

• 한국전자거래학회지
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• 제16권3호
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• pp.169-190
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• 2011

국내 유아식 및 유제품 제조기업의 선두주자인 M사는 출산율의 감소로 인한 시장 규모 축소, 외산 분유의 유입 증가, 심화되는 경쟁사와의 내수시장 경쟁으로 인하여 점점 성장률이 침체되는 경향을 보이고 있었다. 이러한 환경 가운데 M사는 개방형 협업을 통한 서비스 혁신 방법론을 활용하여 새로운 서비스 혁신의 기회를 마련하였다. 그 결과, 외부 이해관계자와의 협업을 통해 서로가 필요한 가치를 상호 교환하고, 외부 자원과 내부의 축적된 기술적 역량을 연결함으로써 차별화된 서비스를 만들 수 있었다. 본 논문에서는 기업 외부의 자원을 활용하여 신 서비스를 개발한 M사의 "개방형 협업을 통한 서비스 혁신 방법론(Open Collaboration Innovation Methodology, OCIM)"의 활용을 중심으로 서술되었다. 또한, 방법론을 적용할 때에 나타난 한계점과 실제 적용할 때 고려해야 할 사항들과 향후 연구의 방향이 수립되었다.

• Environmental Engineering Research
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• 제24권3호
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• pp.434-442
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• 2019

The present study investigated a novel calcium hydroxide-coated dairy manure-derived biochar (Ca-BC) for adsorption of phosphate from water and dairy wastewater. The Ca-BC showed much higher adsorption of phosphate than that of dairy manure-derived biochar. The Ca-BC possessed mainly the calcium hydroxide and various functional groups resulting in high reactivity between phosphate and calcium hydroxide in the Ca-BC. The adsorption of phosphate onto Ca-BC followed pseudo-second order kinetic and Freundlich isotherm models indicating chemisorptive interaction occurred on energetically heterogeneous surface of Ca-BC. The maximum adsorption capacity of the Ca-BC was higher than those of iron oxide and zinc oxide-coated biochars, but lower than those of CaO- and MgO-coated biochars. However, the Ca-BC showed high reactivity per surface area for adsorption of phosphate indicating importance of surface functionalization of biochar. On the other hand, the adsorption of phosphate in dairy wastewater on Ca-BC was lower than that in water owing to competition between other anions in wastewater and phosphate. Overall, the Ca-BC would be a low cost and effective adsorbent for recovery of phosphate from water and wastewater.

• Journal of Forest and Environmental Science
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• 제29권3호
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• pp.194-199
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• 2013

The aim of the present study was to assess the effect of transitional boundary on community composition and soil carbon stock. Five vegetation types were recognized horizontally along the transitional strip based on the dominance of tree species i.e., Pure Anogeissus latifolia forest (P.AL), mixed Pinus roxburghii and Lannea coromandelica forest (M.PR&LC), pure Pinus roxburghii forest (P.PR), mixed Pinus roxburghii and Lannea coromandelica (M.PR&LC) and pure Anogeissus latifolia forest (P.AL). The results revealed that Anogeissus latifolia was reported dominant tree in the outer transitional boundaries of the forest, which reduced dominance of trees towards middle where Pinus roxburghii was found dominant. The soil carbon stock was reported higher in the Anogeissus latifolia dominant forest and reduced with the dominance of Pinus roxburghii in the middle site. Both the species are growing close to one another and competing for survival, but the aggressive nature of Anogeissus latifolia particular in this region may change new growth of Pinus roxburghii and will enhance soil carbon stock. But high anthropogenic pressure on Anogeissus latifolia tree species could be limited chance to further its flourish.

• Journal of Ecology and Environment
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• 제36권3호
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• pp.167-172
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• 2013

A Two-year field experiment was carried out in 2009 and 2010 to evaluate the effects of different densities of wild oat and nitrogen (N) rates on oilseed rape yield and yield components. Experimental design was split plot with three replications. Increased nitrogen rates up to 150 kg N/ha caused an increase in oilseed rape grain yield in weed-free plots (4.26 t/ha; 1t = $10^3kg$), while even at the lowest density of wild oat (15 $plants/m^2$), increasing N fertilizer only up to 50 kg/ha led to a significant increase in grain yield. Wild oat growth was favored by high levels of N. Intraspecific competition reduced biomass, 100-seed weight and number of tillers of wild oat. Both lower N rate and intraspecific competition had negative effect on wild oat growth but the weed was still able to produce a considerable number of tillers and vigorous seeds. It showed that wild oat possess a notable adaptive plasticity and can allocate a greater proportion of its resource toward reproductive organs even under resource shortage conditions and, thus, it may increase the competitive ability of the weed over the crop.Our results suggest that effective weed management should be done to prevent wild oat seed production in crops preceding oilseed rape as well as to control the weed at early season in oilseed rape fields.

• 한국물환경학회지
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• 제20권3호
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• pp.197-205
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• 2004

Batch test methods have developed for a long time to measure kinetic and stoichiometric parameters which are required to perform steady state design and mathematical modelling of activated sludge processes. However, at various So/Xo ratios, abnormal behaviors of ordinary heterotrophic organism in batch tests have been reported in many researches. Thus, in this research, abnormal behaviors of heterotrophs in batch tests were investigated at various So/Xo conditions by measuring and interpreting oxygen utilization rate. As So/Xo ratio increased, the calculated values of maximum specific growth rates, ${\mu}_{H,max}$ and $K_{MP,max}$, increased. However, at a certain point of So/Xo (around 10mgCOD/mgMLAVSS), ${\mu}_{H,max}$ and $K_{MP,max}$ values started to decrease. According to this observation, three prominent behaviours of heterotrophs were identified at various So/Xo conditions. (1) At low So/Xo region (below 5 mgCOD/mgMLAVSS), the oxygen utilization rate of heterotrophs in batch tests were almost stable and consequently yielded lower maximum specific growth rate. (2) At high So/Xo region (up to 5~10 mgCOD/mgMLAVSS), oxygen utilization rate incresed sharply with time and indicated more upward curvature than the predicted OUR with conventional activated sludge model, which consists of single hetetrotrophs group. Thus, in this region, competition model of two organisms, fast-grower and slow-grower, seemed to be appropriate. (3) At extremely high So/Xo region (over 10mgCOD/mgMLAVSS), significant oxygen utilization rate was still observed even after depletion of readily biodegradable COD. This might be caused by retarded utilization of intermediates which were generated by self inhibition mechanism in the process of RBCOD uptake.

• 한국운동역학회지
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• 제21권5호
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• pp.529-540
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• 2011

The purpose of this study was to evaluate the kinematic characteristics of $5^{th}$ and $6^{th}$ hurdle clearances during the final of the 110-m hurdles at the IAAF World Championships, Daegu 2011. To this end, the hurdling motions of the top 4 ranked male hurdlers in the competition were analyzed. A total of 12 cameras were used to record their motions, with a sampling frequency of 120 Hz. The cameras were calibrated using $11{\times}2{\times}1\;m$ control objects that covered all of the lanes (1st~8th lanes). After analyzing all the data, we arrived at the following results. In the take-off phase, all athletes revealed similar take-off times (CT), and similar distances from the take-off to hurdleto (L1) and hurdle to landing (L2). In particular, Turner, ranked $3^{rd}$, had an inconsistent L2 and may need further training to correct it. In the flight phase, Richardson, ranked $1^{st}$, showed the longest flight distance, whereas Xiang, who was ranked $2^{nd}$, showed the highest CG height from the hurdle. For the step patterns, to increase the pitch frequency, Richardson and Xiang used shorter 3-step lengths than Turner and Oliver.