• 한국가축번식학회지
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• 제26권1호
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• pp.33-39
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• 2002

소 체세포 핵이식 시 융합액 내 $Ca^{2+}$ 농도에 따른 융합율, 핵형의 변화, 배 발달율을 검토한 결과는 다음과 같다. 1. 융합액 내 $Ca^{2+}$ 농도를 0.05, 0.1, 0.5 및 1.0 mM로 각기 다르게 처리한 결과, 0.5와 1.0 mM의 CaC$_2$에서 융합율이 80.5 와 84.3%로 나타나 0.05 mM CaCL$_2$에서의 융합율 56.6%에 비하여 유의적(P＜0.01)으로 높았다. 2. 융합액 내 $Ca^{2+}$ 농도에 따른 재구축배의 핵형을 검토한 결과, CaCL$_2$농도 0.05와 0.1 mM 에서는 88.4와 84.0%의 난자가 PCC이후 염색질괴를 형성한 반면, 0.5와 1.0 mM 에서는 54.5와 59.3%가 PCC 형태를 거치지 않고 직접 전핵을 형성하여, $Ca^{2+}$ 농도가 증가함에 따라 PCC 형태를 거치지 않고 직접 전핵을 형성하는 난자의 비율이 증가하였다. 3. 핵이식란의 체외 발육율을 검토한 결과, 1.0 mM CaCl$_2$에서는 배반포 발육율이 30.6%로 나타난 반면, 0.1 mM CaCl$_2$에서 는 20.0%로 나타나 유의적(P＜0.05)인 차이를 나타냈다. 본 연구의 결과는 융합액 내 $Ca^{2+}$농도의 증가가 소 체세포 핵이식란의 융합율 및 배반포 발육율을 향상시킬 수 있음을 시사한다.

• Asian-Australasian Journal of Animal Sciences
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• 제30권11호
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• pp.1620-1632
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• 2017

Objective: This study evaluated the effects of a traditional Chinese medicine formula (TCMF) on muscle fiber characteristics in finishing pigs and the effects of the formula's extract (distilled water, ethyl acetate and petroleum ether extraction) on porcine cell proliferation and isoforms of myosin heavy chain (MyHC) gene expression in myocytes. Methods: In a completely randomized design, ninety pigs were assigned to three diets with five replications per treatment and six pigs per pen. The diets included the basal diet (control group), TCMF1 (basal diet+2.5 g/kg TCMF) and TCMF2 (basal diet+5 g/kg TCMF). The psoas major muscle was obtained from pigs at the end of the experiment. Muscle fiber characteristics in the psoas major muscle were analyzed using myosin ATPase staining. Cell proliferation was measured using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) dye and cytometry. Isoforms of MyHC gene expression were detected by real-time quantitative polymerase chain reaction. Results: The final body weight and carcass weight of finishing pigs were increased by TCMF1 (p<0.05), while the psoas major muscle cross-sectional area was increased by TCMF (p<0.05). The cross-sectional area and diameter of psoas major muscle fiber Ι, IIA, and IIB were increased by TCMF2 (p<0.05). The cross-sectional area and fiber diameter of psoas major muscle fiber IIA and IIB were increased by diet supplementation with TCMF1 (p<0.05). Psoas major muscle fiber IIA and IIB fiber density from the pigs fed the TCMF1 diet and the type IIB fiber density from the pigs fed the TCMF2 diet were lower compared to pigs fed the control diet (p<0.05). Pigs fed TCMF2 had a higher composition of type Ι fiber and a lower percentage of type IIB fiber in the psoas major muscle (p<0.05). The expression levels of MyHC Ι, MyHC IIa, and MyHC IIx mRNA increased and the amount of MyHC IIb mRNA decreased in the psoas major muscle from TCMF2, whereas MyHC Ι and MyHC IIx mRNA increased in the psoas major muscle from TCMF1 (p<0.05). Peroxisome proliferator-activated receptor ${\gamma}$ $coactivator-1{\alpha}$ and CaN mRNA expression in the psoas major muscle were up-regulated by TCMF (p<0.05). Porcine skeletal muscle satellite cell proliferation was promoted by $4{\mu}g/mL$ and $20{\mu}g/mL$ TCMF water extraction (p<0.05). Both $1{\mu}g/mL$ and $5{\mu}g/mL$ of TCMF water extraction increased MyHC IIa, MyHC IIb, and MyHC IIx mRNA expression in porcine myocytes (p<0.05), while MyHC Ι mRNA expression in porcine myocytes was decreased by $5{\mu}g/mL$ TCMF water extraction (p<0.05). Porcine myocyte MyHC Ι and MyHC IIx mRNA expression were increased, and MyHC IIa and MyHC IIb mRNA expression were down-regulated by $5{\mu}g/mL$ TCMF ethyl acetate extraction (p<0.05). MyHC Ι and MyHC IIa mRNA expression in porcine myocytes were increased, and the MyHC IIb mRNA expression was decreased by $1{\mu}g/mL$ TCMF ethyl acetate extraction (p<0.05). Four isoforms of MyHC mRNA expression in porcine myocytes were reduced by $5{\mu}g/mL$ TCMF petroleum ether extraction (p<0.05). MyHC IIa mRNA expression in porcine myocytes increased and MyHC IIb mRNA expression decreased by $1{\mu}g/mL$ in a TCMF petroleum ether extraction (p<0.05). Conclusion: These results indicated that TCMF amplified the psoas major muscle cross-sectional area through changing muscle fiber characteristics in finishing pigs. This effect was confirmed as TCMF extraction promoted porcine cell proliferation and affected isoforms of MyHC gene expression in myocytes.

• Nutrition Research and Practice
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• 제5권4호
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• pp.288-293
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• 2011

In this study, we investigated the effects of the ethanol extract of aerial parts of Raphanus sativus L. (ERL) on breast cancer cell proliferation and gene expression associated with cell proliferation and apoptosis in MDA-MB-231 human breast cancer cells. The MDA-MB-231 cells were cultured in the presence or absence of various concentrations (100, 200, or 300 ${\mu}g$/mL) of ERL. ERL significantly decreased cell proliferation after 48 h of incubation (P < 0.05). The protein and mRNA expression of $ErbB_2$ were decreased significantly in a dose-dependent manner (P < 0.05). The protein expression of $ErbB_3$ was decreased significantly at an ERL concentration of 300 ${\mu}g$/mL (P < 0.05), and mRNA expression of $ErbB_3$ was decreased significantly in a dose-dependent manner (P < 0.05). The protein expression of Akt was decreased significantly at the ERL concentration of 200 ${\mu}g$/mL (P < 0.05), and the protein expression of pAkt was decreased significantly in a dose-dependent manner (P < 0.05). The mRNA expression of Akt was decreased significantly at the ERL concentration of 200 ${\mu}g$/mL ERL (P < 0.05). The protein and mRNA expression of Bax were increased significantly at ERL concentrations of 200 ${\mu}g$/mL or higher (P < 0.05). The protein expression of $Bcl_2$ was increased significantly at ERL concentrations of 100 ${\mu}g$/mL or higher (P < 0.05), and mRNA expression of $Bcl_2$ was increased significantly at an ERL concentration of 300 ${\mu}g$/mL (P < 0.05). In conclusion, we suggest that Raphanus sativus, L. inhibits cell proliferation via the ErbB-Akt pathway in MDA-MB-231 cells.

• Asian-Australasian Journal of Animal Sciences
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• 제27권11호
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• pp.1584-1592
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• 2014

The objective was to evaluate the ingestive behavior of crossbred heifers finished on a Brachiaria brizantha cv. Marandu pasture receiving four levels of glycerin in their supplementation. Thirty-six crossbred heifers with average initial weight of $264.83{\pm}3.83kg$ and 20 months of age were distributed into a completely randomized design with four treatments and nine replications: control (0%), 4.82%, 10.12%, and 15.56% glycerin in the dry matter. The grazing time reduced linearly (p<0.05), whereas the time spent on activities like rumination, idleness, trough and total chewing time were quadratically affected (p<0.05). Bite rate and number of bites/day were quadratically influenced (p<0.05). The number of bites/swallowed cud and the number of bites/minute, however, increased linearly (p<0.05). Although the time spent on each cud and number of chews per cud were not affected (p>0.05).The number of rumination periods reduced linearly (p<0.05), whereas the number of grazing, idle and trough periods, and the times per grazing, idle, rumination and trough periods were quadratically affected (p<0.05). The feed and rumination efficiencies of the dry matter, non-fibrous carbohydrates, pasture dry matter and concentrate were quadratically affected (p>0.05) whereas the feed efficiency of neutral detergent fiber reduced linearly (p<0.05). Addition of glycerin in substitution of corn in supplements for animals managed on pastures does not influenced feed intake, but reduces the grazing time and increases the idle time. The supplementation also improves feed and rumination efficiencies.

• 육가공
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• 통권36호여름호
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• pp.41-60
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• 2009

시중에 유통 중인 브랜드 돈육의 품질 특성을 조사하기 위해 3개 브랜드(Y처리구 : 재래돼지 $\times$멧돼지, 맥강과 한약제 급여 ; J처리구 : LY$\times$D, 무항생제, 생균제 급여; M처리구 : LY$\times$D) 돈육 등심을 구매하여 품질 특성을 분석하였다. 수분과 조회분 함량은 Y처리구가 J와 M 처리구에 비해 높았지만, 조지방 함량은 J처리구가 Y와 M처리구에 비해 현저하게 낮았다(p<0.05). Y처리구의 미경산돈이 거세돈보다 수분과 조지방 함량이 현저하게 높았다(p<0.05) Y처리구의 미경산돈의 pH는 J와 M처리구의 미경산돈에 비해 현저하게 높았다(p<0.05) 돈육의 L(명도)값과 $W^*$(백색도)값은 Y처리구가 J와 M처리구에 비해 현저하게 낮았다(p<0.05) 조직 특성 중 탄력성은 J처리구가 Y와 M처리구에 비해 현저하게 높았다(p<0.05). 관능적 점수는 Y처리구가 J와 M처리구에 비해 높았고, M처리구의 미경산돈은 거세돈에 비해 다즙성이 현저하게 높았다(p<0.05). 지방산 조성 중 linoleic acid와 arachidonic acid 함량은 Y처리구가 J와 M 처리구에 비해 높았지만 palmitic acid, pamitoleic acid, stearic acid 및 oleic acid 함량은 낮았다. 포화지방산 함량은 Y처리구가 J와 M처리구에 비해 낮았지만, 불포화지방산, 필수지방산, 불포화지방산/포화지방산 비, 필수지방산/포화지방산 비 및 필수지방산/불포화 지방산 비는 가장 높은 함량을 나타내었다. 성별 간에는 M처리구에서 거세돈이 미경산돈에 비해 stearic acid 함량이 현저하게 높았다(p<0.05). 이상의 결과를 종합해 볼 때 재래돼지$\times$멧돼지를 교잡하여 맥강과 한약재를 급여한 Y처리구가 J와 M처리구에 비해 육의 이화학 특성과 관능적 특성에서 우수한 것으로 나타났다.

• 한국축산식품학회지
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• 제28권4호
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• pp.470-479
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• 2008

시중에 유통 중인 브랜드 돈육의 품질 특성을 조사하기 위해 3개 브랜드(Y처리구 :재래돼지$\times$멧돼지, 맥강과 한약제 급여; J처리구 LY$\times$D, 무항생제, 생균제 급여; M처리구 :LY$\times$D)돈육 등심을 구매하여 품질 특성을 분석하였다. 수분과 조회분 함량은 Y처리구가 J와 M처리구에 비해 높았지만, 조지방 함량은 J처리구가 Y와 M처리구에 비해 현저하게 낮았다(p<0.05). Y처리구의 미경산돈이 거세돈보다 수분과 조지방 함량이 현저하게 높았다(p<0.05). Y 처리구의 미경산돈의 pH는 J와 M처리구의 미경산돈에 비해 현저하게 높았다(p<0.05).돈육의 L(명도)값과 W*(백색도)값은 Y처리구가 J와 M처리구에 비해 현저하게 낮았다(p<0.05). 조직 특성 중 탄력성은 J처리구가 Y와 M처리구에 비해 현저하게 높았다(p<0.05). 관능적 점수는 Y처리구가 J와 M처리구에 비해 높았고, M처리구의 미경산돈은 거세돈에 비해 다즙성이 현저하게 높았다(p<0.05).지방산 조성 중 linoleic acid와 arachidonic acid 함량은 Y처리구가 J와 M처리구에 비해 높았지만 palmitic acid, pamitoleic acid, stearic acid 및 oleic acid 함량은 낮았다. 포화지방산 함량은 Y처리구가 J와 M처리구에 비해 낮았지만, 불포화지방산, 필수지방산, 불포화지방산/포화지방산 비, 필수지방산/포화지방산 비 및 필수지방산/불포화지방산 비는 가장 높은 함량을 나타내었다. 성별 간에는 M처리구에서 거세돈이 미경산돈에 비해 stearic acid 함량이 현저하게 높았다(p<0.05). 이상의 결과를 종합해 볼 때 재래돼지$\times$멧돼지를 교잡하여 맥강과 한약재를 급여한 Y처리구가 J와 M처리구에 비해 육의 이화학 특성과 관능적 특성에서 우수한 것으로 나타났다.

• 한국가금학회지
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• 제33권3호
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• pp.189-194
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• 2006

본 연구의 목적은 천연 미네랄 제제의 첨가가 산란계의 생산성, 난질 및 혈액과 난황 내 미네랄 함량에 미치는 영향을 알아보고자 실시하였다. 72주령 Hy-line Brown 240수를 공시하였고, 6주간 사양 시험을 실시하였다. 처리구는 1) Control(CON), 2) Control + 3% 키토산 + 0.50% 천연미네랄 제제 (M 0.5), 3) Control + 3% 키토산 + 0.10% 천연미네랄 제제 (M 1.0), 4) Control +3% 키토산 + 0.15% 천연미네랄 제제(m1.50)로 총 4 처리를 하여 처리당 10반복, 반복당 6수씩 임의 배치하였다. 산란율은 M1.5 처리구가 대조구, M1.0 처리구와 비교하여 유의적으로 높게 나타났다(P<0.05). 하지만 난중은 M0.5 처리구가 다른 처리구와 비교하여 유의적으로 높게 나타났다(P<0.05). 난각 강도는 처리구와 대조구간에 차이를 보이지 않았으나, 난각 두께는 M1.5 처리구가 대조구 및 다른 처리구와 비교하여 유의적으로 높게 나타났다(P<0.05). 난황색은 미네랄 처리구에서 첨가 수준의 증가에 따라 감소하는 경향을 보였다. Haugh unit은 대조구와 M1.0 처리구가 가장 높게 나타났다(P<0.05). 난황 내 K의 함량은 M1.5 처리구가 대조구와 비교하여 증가하였다(P<0.05). 그러나 난황 내 Ca과 Fe 함량에 있어서 처리구간의 유의적인 차이는 없었다(P<0.05). 혈액 내 Ca과 Fe 함량은 M1.5 처리구에서 대조구와 비교하여 높게 나타났다(P<0.05). 따라서 산란계에서 1.5% 천연 복합 미네랄 제제의 급여는 산란율, 난각 두께를 개선시켰고, 혈액 및 난황 내 미네랄 조성을 변화시켰다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권10호
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• pp.1587-1593
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• 2007

A single factorial experiment was conducted to test the effects of three dietary levels of energy on mRNA expression of fatty acid synthase (FAS-mRNA) and hormone-sensitive lipase (HSL-mRNA) and their association with intramuscular fat in finishing pigs. 72 crossbred (Large $White{\times}Rongchang$) barrows with an average initial body weight of 20.71 (s.e. 0.1) kg, were randomly allotted to three dietary treatments (11.75, 13.05 and 14.36 MJ DE/kg) and fed until slaughtered at 100 or 101 kg. The diets were iso-nitrogenous and iso-essential amino acids. The growth performances including the duration of finishing were changed linearly (p<0.05) or quadratically (p<0.05) with increased dietary energy levels. The effects of dietary energy content on the percentage of external fat, intramuscular backfat and the fat thickness were linear (p<0.05). The content of dietary energy increased FAS-mRNA linearly or quadratically, while HSL-mRNA decreased linearly or quadratically in backfat and Longissmus dorsi muscle. Meanwhile, significant positive correlations (p<0.05) were found between energy level and intramuscular fat, FAS-mRNA or the ratio of FAS-mRNA to HSL-mRNA, between the ratio of FAS-mRNA to HSL-mRNA and intramuscular fat. However, the correlations between HSL mRNA and dietary energy or intramuscular fat were negative (p<0.05). The results indicated that dietary energy level regulates lipid accumulation, especially intramuscular fat, possibly by modulating the mRNA of FAS and HSL together rather than individually.

• 대한한의학회지
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• 제19권2호
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• pp.450-467
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• 1998

To research the characteristics of ion currents induced by inhibitory and excitatory herbs of oriental medicine, we used nystatin-perforated patch clamp technique under voltage clamp condition in periaqueductal gray neuron dissociated from Sprauge-Dawley rat, 10-15 days old. The results are as follows. 1. Ion current induced by $10mg/m{\ell}$ of Bupleuri Radix was inhibited $59.50{\pm}4.29%$ by $10^{-4}M$ bicuculline(p>0.01) but inhibition of $10.75{\pm}4.77%$ by $10^{-4}M$ tubocurarine and $4.75{\pm}4.23%$ by $10^{-4}M$ verapamil had no statistical significance(p>0.05). So ion current induced by Bupleuri Radix revealed only GABA induced $Cl^-$ current, not acetylcholine and $Ca^{2+}$ current. 2. Ion current induced by $20mg/m{\ell}$ of Coptidis Rhizoma was inhibited $47.20{\pm}7.88%$ by $10^{-4}M$ bicuculline(p<0.01) but $3.20{\pm}2.33%$ inhibition by $10^{-4}M$ tubocurarine and $1.00{\pm}1.00%$ inhibition by $10^{-4}M$ verapamil had no significance(p>0.05). So ion current induced by Coptidis Rhizoma revealed only GABA induced $Cl^-$ current, not acetylcholine and $Ca^{2+}$ current. 3. Ion current induced by $20mg/m{\ell}$ of Ecliptae Herba was inhibited $55.00{\pm}4.92%$ by $10^{-4}M$ bicuculline (p<0.01), and also inhibited $15.00{\pm}4.26%$ by $10^{-4}M$ tubocurarine(p<0.05), but inhibition of $6.00{\pm}3.03%$ by $10^{-4}M$ verapamil had no significance(p>0.05). So ion current induced by Ecliptae Herba showed GABA activated $Cl^-$ current and acetylcholine activated cation current, not $Ca^{2+}$ current 4. Ion current induced by $5mg/m{\ell}$ of Liriopis Tuber was inhibited $15.20{\pm}4.57%$ by $10^{-4}M$ bicuculline<0.05) and also inhibited $14.00{\pm}3.00%$ by $10^{-4}M$ tubocurarine(p<0.05), but inhibition of $5.20{\pm}4.80%$ by $10^{-4}M$ verapamil had no significance(p>0.05). So ion current induced by Liriopis Tuber showed GABA. activated $Cl^-$ current and acetylcholine activated cation current, not $Ca^{2+}$ current. 5. Ion current induced by $5mg/m{\ell}$ of Aconiti Tuber was inhibited $97.00{\pm}1.34%$ by $10^{-4}M$ bicuculline(p<0.01), $80.00{\pm}9.83%$ by $10^{-4}M$ tubocurarine(p<0.01), and $24.00{\pm}6.18%$ by $10^{-4}M$ verapamil(p<0.05). So ion current induced by Aconiti Tuber revealed GABA activated $Cl^-$ current and acetylcholine activated cation current and $Ca^{2+}$ current. 6. Ion current induced by $10mg/m{\ell}$ of Zingiberis Rhizoma was inhibited $33.00{\pm}7.43%$ by $10^{-4}$ bicuculline(p<0.05), $10.20{\pm}1.83%$ by $10-^{-4}M$ tubocurarine(p<0.01), and $14.00{\pm}2.16%$ by $10^{-4}M$ verapamil(p<0.01) So ion current induced by Zingiberis Rhizoma revealed GABA activated $Cl^-$ current and acetylcholine activated cation outtent and $Ca^{2+}$ current. 7. Ion current induced by $10mg/m{\ell}$ of Boshniakiae Herba was inhibited $65.00{\pm}13.75%$ by $10^{-4}M$ bicuculline(p<0.05), $38.00{\pm}9.24%$ by $10^{-4}M$ tubocurarine(p<0.05), and $33.25{\pm}7.42%$ by $10^{-4}M$ verapamiHp<0.05). So ion current induced by Bpshniakiae Herba revealed GABA activated $Cl^-$ current and acetylcholine activated cation current and $Ca^{2+}$ current. These results suggest that a point of difference between inhibitory and excitatory herbs is existence of$Ca^{2+}$ current.

• Applied Biological Chemistry
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• 제34권2호
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• pp.117-124
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• 1991

기주 식물에 대한 인산공급이 근류의 인산 형태와 bacteroid의 인산흡수에 미치는 영향을 살펴보고자 B. japonicum MN 110을 접종한 대두식물을 1.0, 0.25, 0.05 mM-P 조건에서 재배하였다. 1.0, 0.25, 0.05 mM-P 조건에서 재배한 대두식물의 근류 Pi 농도는 각각 4.1, 2.5, 2.0 mM이었으며 인산공급이 근류의 Pi, SOP, lOP의 분포에는 유의성 있는 영향을 미치지 못하였다. 0.25, 0.05 mM-P 조건에서 재배한 대두식물의 유엽 Pi 농도는 대조구(1.0 mM-P)의 각각 33%, 20%이었으며 노엽 Pi 농도는 대조구의 각각 16%, 7%였다. 잎에서는 인산 결핍이 전인산에 대한 Pi의 분포를 감소시켰으나 lOP의 분포는 증가시켰다. 근류내의 bacteroid의 수는 $0.87-1.30{\times}10^{11}\;/GFW\;nodule$이었으며, 인산흡수도는 15-16 pmoles /min/$10^8$ bacteroid로 기주식물의 인산공급에는 영향을 받지 않았다 이러한 결과는 인산결핍 대두식물의 근류내 Pi농도는 bacteroid의 생육과 번식에 충분하며 인산결핍에 의하여 기주식물의 생육이 저해될 때에도 bacteroid의 인산흡수는 억제상태에 있음을 나타낸다.