• Parasites, Hosts and Diseases
• /
• v.35 no.4
• /
• pp.271-276
• /
• 1997

We tried to compare the three kinds oi Metagonimur species. M. Wokognulci, Ifetafonimus Miyata type, and M. tnknhashii, which were Know to be distributed in Korea with polymerase chain reaction based-restriction fragment length polymorphism (PCR- RFLP) patterns. We amplified the internal transcribed spacer 1 (ITSI) site of ribosomal RNA and mitochondrial cytochrome c oxidase I (mCOI) gene. The restriction patterns of ITSI gene loth Rsc I, ALu I and Msp I showed multiple fragmented bands of different sizes between three kinds of Metcgonimus. In case of mc01 gene, Rsc I and Alu I enzymes produced differentially fragmented band patterns. According to the parsimony analysis of PCR-RFLP patterns, the estimated genetic divergence between M Wokognwai and Metasoninus Miyata type was 0.034880, between Metusoninus Miyata type and M. tckc- hushii was 0.028098, between M. wokogawai and M. tnkahashii was 0.018179. It is suggested that Metasonimus Miyata type may be separate species and evolutionize at the older time than the other two species.

• Parasites, Hosts and Diseases
• /
• v.34 no.1
• /
• pp.7-14
• /
• 1996

Taxonomic problems of Metagoninus spry. in Korea were investigated. Metacercariae of various freshwater fish species - PlecoBlossus cltiuelis, Carnssius aurctus, Zacco platypus, Zncco temmincki, Opscriichthws bidens - were collected from different localities in Korea and experimentally fed to golden hamsters. Observation of recovered adult worms showed that PLeco91ossus nltiuelis was infected with metacercariae of both M. vokognwni and M. toknhoshii. C. auratus was infected with metacercariae of M. takchcshii and Z. platvpf, Z. temmincki, O. binens were infected only with metacercariae of Metofonimuf Miyata type. From the inferences about the morphological characteristics, host specificities and occurrence patterns in infected animals, Metogonimus Miyata type is considered to be an independent group.

• Parasites, Hosts and Diseases
• /
• v.31 no.2
• /
• pp.99-108
• /
• 1993

An epidemiological study of Metogonimus infection was undertaken along the upper reaches of the Nmhn River, with special consideration on the species (type) of the worms collected from humans. Eggs of Metogonimus spp. were detected from 15 (9.7%) of 154 people examined in Umsong-gun, and from each Infected person (5 cases) 6.015- 24,060 worms (mean 13,2331 were recovered after treatment with praziquantel (10 mg/kg). Eggs were also detected from 37 (48.1% of 77 people In Yongwol-gun, from whom (27 casesl 1-4,965 worms (mean 1,2151 were collected. The worm from Umsong-gun consisted of both Metagonimus Miyata type and Metagonimus takohashii, whereas those from Yongwol-gun consisted of only Metqsonimus Miyata type. When the uterine eggs of the two kinds and M. yokogawoi (obtained from people In Tamjin River basin) were morphologically compared, it was suggested that the egg size should be a good Indicator for discrlmination of the species or type. The source of human Infection was proved to be fresh water fleshes: 49 of 52 Znsco plntvpus examined, 6 of 8 Hemibcrbus loniroskis, 13 of 15 Pseunogobio esuinl:, 4 of 6 Odontobutis obscura intemfptn, and 17 of 18 Corqssiw carassiw were found Infected with Metosonimw metacercariae. From the results, it is concluded that the upper reaches of the Namhan River are endemic foci of Metogonimn Miyata type and M. takahashii.

• Parasites, Hosts and Diseases
• /
• v.32 no.3
• /
• pp.149-156
• /
• 1994

An epidemiological study fur the Intestinal trematode infection of the villagers was done In Koje-myon, Kochang-gun, Kyongsangnam-do, Korea in March, 1994. Of 116 stool specimens examined, total helmlnthic ova positive cases were 13 (11.2%) and cumulative ova positive cases 21 (18.1%): Echinostoma hortense 11 cases (9.5%), Metngonimus sp. 6 cases (5.2%), and Clonorchis sinensis 4 cases (3.4%). After the treatment and purgation, variable numbers of E. hokene, 6 to 227 per person, were collected from 7 echlnostome egg Positive cases, together with M. tokohoshii In 6 cases. Three adult flukes of E. cinetorchis were collected from one person and one Stellantchasmw jnlcatus was recovered from another case. This mountainous village was proved to be one of the endemic foci of echlnostomlasis In Korea.

• Parasites, Hosts and Diseases
• /
• v.26 no.3
• /
• pp.207-214
• /
• 1988

The Hongcheon river system knows down through the Hongcheon area of Kangwon-do, and reaches to the Cheongpyeong "Dam in Kyonggi.do. Stool specimens from the inhabitants residing along the Hongcheon river basin were examined to detect infection rates of Metagonimus sp. , and the intermediate hosts were collected to detect larval stages. The results obtained were as follows: 1. Thirty-nine (33 males and 6 females) out of 529 (314 males and 215 females) inhabitants were infected with Metagonimus sp., showing a total positive rate of 7.4%. 2. In eight areas surveyed, the specimens from Kulji.ri of Bukbang-myon at the middle part of the river showed the highest positive rate of 26.9% (14 positives out of 52) (males; 38.2%), The specimens from Mogog-ri of Seo-myon at the downstream of the river showed a positive rate of 10.4%(13 positives out of 125) (males; 12.6%). The positive rates in other regions were less than 10%. 3. The density of the first intermediate host, Semisulcospira sp., was the highest in Kulji.ri of Bukbang-myon (10~20 snails per $m^2$), and the infection rate of Metagonimus cercariae in the snails was 10.7%(13 positives out of 121 snails). 4. The infection rate of Metagonimus metacercariae in Zacco platypus, the freshwater Bish favorably eaten raw by the inhabitants, was 68.25 (30 positives out of 44 fishes), and most metacercariae were detected under the scales (89.95). 5. Adult flukes were obtained from the small intestine of a rat, 15 days after infection with the metacercariae obtained from Z. platypus. These adult cukes were identified to be the same species as thcse obtained from human hosts. By this survey, new endemic areas of Metagonimus infection were discovered along the Hongcheon river basin and the main source of infection was the fresh water ash, Z. platypus.