• 한국유기농업학회지
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• 제6권1호
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• pp.127-132
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• 1997

This experiment was carried out to examine the effect of various functional materials such as bioceramic podwers, crushed shells and elvanites supplemented to the each substrate on the seedlings growth of rices. The rice seedlings were grown in pots filled with substrates supplemented with bioceramic podwers, crushed shells and elvanites. The growth of rice seedlings in terms of plant height, stem diameter, root length and leaf width, plant fresh and dry weight was promoted by adding the bioceramic powders (2 to 3g/kg), crushed shells (10g/kg) or elvanites (20 to 40g/kg). Plant height was also promoted by the adding of bioceramic powder from 16 days after treatment, whereas crushed shells and elvanites from 10 days after treatment. Especially, root growth was greatly influenced by bioceramic powder, whereas the shoot growth(leaves and stem) was stimulated by the crushed shells and elvanites supplemented into each substrate. In the field, plant growth in terms of plant height, leaf length and leaf width were also influenced by crushed shells and elvanites at 74 days after treatment. The growth of rices in terms of tiller number, spikelets, panicles and spikelets/panicle was incresed by adding the crushed shells and elvanites from 100 to 200g per m2.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1999년도 제13회 식물생명공학심포지움 New Approaches to Understand Gene Function in Plants and Application to Plant Biotechnology
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• pp.41-44
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• 1999

Magnaporthe grisea (Hebert) Barr (anamorph: Pyricularia grisea) is a typical heterothallic Ascomycete and the causal agent of rice blast, one of the most destructive diseases on rice (Oryza sativa L.) worldwide. The interactions between cells of the pathogen and those of the host involve a complex of biological influences which can lead to blast disease. The early stages of infection process in particular may be viewed as a sequence of discrete and critical events. These include conidial attachment, gemination, and the formation of an appressorium, a dome-shaped and melanized infection structure. Disruption of this process at any point will result in failure of the pathogen to colonize host tissues. This may offer a new avenue for developing innovative crop protection strategies. To recognize and capture such opportunities, understanding the very bases of the pathogenesis at the cellular and molecular level is prerequisite. Much has been learned about environmental cues and endogenous signaling systems for the early infection-related morphogenesis in M. grisea during last several years. The study of signal transduction system in phytopathogenic filamentous fungi offers distinct advantages over traditional mammalian systems. Mammalian systems often contain multiple copies of important genes active in the same tissue under the same physiological processes. Functional redundancy, alternate gene splicing, and specilized isoforms make defining the role of any single gene difficult. Fungi and animals are closely related kingdoms [3], so inferences between these organisms are often justified. For many genes, fungi frequently possess only a single copy, thus phenotype can be attributed directly to the mutation or deletion of any particular gene of interest.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1985년도 워크샵 및 심포지엄 북한산국립공원의 식생
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• pp.19-22
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• 1985

Magnaporthe grisea (Hebert) Barr (anamorph: Pyricularia grisea) is a typical heterothallic Ascomycete and the causal agent of rice blast, one of the most destructive diseases on rice (Oryza sativa L.) worldwide. The interactions between cells of the pathogen and those of the host involve a complex of biological influences which can lead to blast disease. The early stages of infection process in particular may be viewed as a sequence of discrete and critical events. These include conidial attachment, gemination, and the formation of an appressorium, a dome-shaped and melanized infection structure. Disruption of this process at any point will result in failure of the pathogen to colonize host tissues. This may offer a new avenue for developing innovative crop protection strategies. To recognize and capture such opportunities, understanding the very bases of the pathogenesis at the cellular and molecular level is prerequisite. Much has been learned about environmental cues and endogenous signaling systems for the early infection-related morphogenesis in M. grisea during last several years. The study of signal transduction system in phytopathogenic filamentous fungi offers distinct advantages over traditional mammalian systems. Mammalian systems often contain multiple copies of important genes active in the same tissue under the same physiological processes. Functional redundancy, alternate gene splicing, and specilized isoforms make defining the role of any single gene difficult. Fungi and animals are closely related kingdoms [3], so inferences between these organisms are often justified. For many genes, fungi frequently possess only a single copy, thus phenotype can be attributed directly to the mutation or deletion of any particular gene of interest.

• Bulletin of the Korean Chemical Society
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• 제33권12호
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• pp.4169-4172
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• 2012

To elucidate the roles of cysteine residues in rice Phi-class GST F3, in this study, all three cysteine residues were replaced with alanine by site-directed mutagenesis in order to obtain mutants C22A, C73A and C77A. Three mutant enzymes were expressed in Escherichia coli and purified to electrophoretic homogeneity by affinity chromatography on immobilized GSH. The substitutions of Cys73 and Cys77 residues in OsGSTF3 with alanine did not affect the glutathione conjugation activities, showing non-essentiality of these residues. On the other hand, the substitution of Cys22 residue with alanine resulted in approximately a 60% loss of specific activity toward ethacrynic acid. Moreover, the ${K_m}^{CDNB}$ value of the mutant C22A was approximately 2.2 fold larger than that of the wild type. From these results, the evolutionally conserved cysteine 22 residue seems to participate rather in the structural stability of the active site in OsGSTF3 by stabilizing the electrophilic substrates-binding site's conformation than in the substrate binding directly.

• Carbon letters
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• 제9권3호
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• pp.188-194
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• 2008

Porous carbon materials synthesized from various plant derived precursors i.e. seeds of [Castor (Ricinus communis), Soap nut (Sapindus sp.), Cashew-nut (Semecarpus anacardium), Jack fruit (Artocarpus heterophyllus), Safflower (Carthamus tinctorius), Ambadi (Crotolaria juncea), Neem (Azadirachta indica), Bitter Almond (Prunus amygdalus), Sesamum (Sisamum indicum), Date-palm (Phoenix dactylifera),Canola (Brassica napus), Sunflower (Helianthus annulus)] and fibrous materials from [Corn stem- (Zea mays), Rice straw (Oryza sativa), Bamboo (Bombax bambusa) and Coconut fibers (Cocos nucifera)] were screened to make supercapacitor in 5M KOH solution. Carbon material obtained from Jack fruit seeds (92.0 F/g), Rice straw (83.0 F/g), Soap nut seeds (54.0 F/g), Castor seeds (44.34 F/g) and Bamboo (40.0 F/g) gave high capacitance value as compared to others. The magnitude of capacitance value was found to be inversely proportional to the scan rate of measurement. It is suggested that carbon material should possess large surface area and small pore size to get better value of capacitor. Moreover, the structure of carbon materials should be such that majority of pores are in the plane parallel to the plane of electrode and surface is fluffy like cotton ball.

• 한국육종학회지
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• 제43권4호
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• pp.252-259
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• 2011

Conventional staining and fluorescence in situ hybridization (FISH) karyotypes of the non-genetically modified (GM) parental rice line, 'Nakdong' (Oryza sativa L. japonica), and its four GM rice lines, LS28 (event LS30-32-20-1), Cry1Ac1 (event C7-1-9-1), and LS28 ${\times}$ Cry1Ac1 (events L/C1-1-3-1 and L/C1-3-1-1) were analyzed using 5S and 45S rDNAs as probes. Both parental and transgenic lines were diploids (2n=24) with one satellite chromosome pair. The lengths of the prometaphase chromosomes ranged from 1.50 to $6.30{\mu}m$. Four submetacentric and eight metacentric pairs comprised the karyotype of 'Nakdong' and its four GM lines. One pair of 5S rDNA signals was detected near the centromeric region of chromosome g in both the parental and transgenic lines. The 45S rDNA signals were detected on the secondary constrictions of the satellite chromosome pair in both the parental and transgenic lines. There was no significant difference in chromosome size, length, and composition between 'Nakdong' and its four GM lines. This research was conducted as a preliminary study for chromosomal detection of transgenes in GM rice lines and would be useful for their breeding programs.

• The Plant Pathology Journal
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• 제38권5호
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• pp.490-502
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• 2022

Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight (BLB) disease in rice (Oryza sativa L.) and it is among the most destructive pathogen responsible for severe yield losses. Potential bacterial biocontrol agents (BCAs) with plant growth promotion (PGP) abilities can be applied to better manage the BLB disease and increase crop yield, compared to current conventional practices. Thus, this study aimed to isolate, screen, and identify potential BCAs with PGP abilities. Isolation of the BCAs was performed from internal plant tissues and rhizosphere soil of healthy and Xoo-infected rice. A total of 18 bacterial strains were successfully screened for in vitro antagonistic ability against Xoo, siderophore production and PGP potentials. Among the bacterial strains, 3 endophytes, Bacillus sp. strain USML8, Bacillus sp. strain USML9, and Bacillus sp. strain USMR1 which were isolated from diseased plants harbored the BCA traits and significantly reduced leaf blight severity of rice. Simultaneously, the endophytic BCAs also possessed plant growth promoting traits and were able to enhance rice growth. Application of the selected endophytes (BCAs-PGP) at the early growth stage of rice exhibited potential in suppressing BLB disease and promoting rice growth.

• 농업과학연구
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• 제6권2호
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• pp.105-111
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• 1979

몇가지 Alfalfa 품종(品種)의 근류균(根瘤菌)에 대(對)한 친화성(親和性) 및 접종효과(接種效果)를 구명(究明)코자 시험(試驗)하였던 바 결과(結果)를 요약(要約)하면 다음과 같다. 1. 접종균주간(接種菌株間)에는 근류형성성(根瘤形成性)에 현저(顯著)한 차이(差異)를 보였으며 Rhizobium meliloti SU47균주(菌株)는 Luna, Von arnims altd bost 등(等) 두 품종(品種)에만 근류(根瘤)를 형성(形成)하였으며 M1균주(菌株)는 전품종(全品種)에서 근류(根瘤)를 형성(形成)치 않았고 M14균주(菌株)는 전품종(全品種)에서 근류(根瘤)를 형성(形成)하였다. 2. 근류착생수(根瘤着生數)는 Franks hangmeiler 품종(品種)이 가장 많았고 그 다음이 Galilee 품종(品種)이었으며 Elga 품종(品種)이 가장 적었다. 3. 초장(草長) 및 지상부건물중(地上部乾物重)은 공시품종(供試品種) 모두 M14균주접종구(菌株接種區)가 가장 컸고 그 다음이 질소대조구(窒素對照區)였으며 M1접종구(接種區)와 대조구(對照區)에서 가장 작았고 근장(根長) 및 지하부건물중(地下部乾物重)은 품종간(種間)및 처리간(處理間)에 유의성(有意性)을 인정(認定)할 수 없었다. 4. 근류착생수(根瘤着生數)와 초장(草長) 및 근장(根長) 그리고 지상부건물중(地上部乾物重)과 근건물중(根乾物重)과는 상관관계(相關關係)를 인정(認定)할 수 없었다.

• KSBB Journal
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• 제20권4호
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• pp.278-284
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• 2005

식물세포의 느린 생장과 낮은 생산량의 이유로 지금까지 는 주로 미생물이나 동물세포에서 유전자 재조합 단백질을 생산하여 왔다. 그러나 저렴한 배지 가격, 동물 유래 바이러스 감염 위험성으로부터의 안정성, glycosylation 등의 post-translational modification이 가능하다는 장점들로 인하여 최근 들어 식물세포배양은 생물학적 활성을 가진 고부가가치의 단백질을 생산하는데 많이 이용되고 있다. 본 연구에서는 생장배지에 첨가했던 sucrose의 소비와 induction 배지로의 교환에서 오는 배지내의 삼투압을 조절하여 hCTLA4-Ig의 생산성을 높이고자 하였다. 다양한 삼투압 조절제 첨가 실험을 통해 sorbitol을 선별하고, 40 mM의 sorbitol 첨가에서 상대적으로 높은 생존도와 induction 후 7일째 대조구보다 1.7배 높은 생산성을 확인하였다. 또한, 저농도의 glucose 첨가를 통한 생산성 증대에 있어서는 8 mM glucose에서 induction 이후에도 높은 세포농도를 유지하면서 최대 37.3 mg/L까지 hCTLA4-Ig 생산량을 증가시켰다. 5-L bioreactor에서 회분식 배양과 induction시의 hCTLA4-Ig 생산량을 비교한 결과 induction시 배양 18일째 최고 45.3 mg/L까지 높일 수 있었으며, 회분식 배양에 비해 2.1배 증가됨을 확인하였다.

• 생물환경조절학회지
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• 제22권3호
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• pp.228-233
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• 2013

본 실험은 식물공장시스템에서 인공광원인 백색 LED의 광도와 광주기에 따른 '선홍적축면'의 생육조건을 구명하고자 수행하였다. 그리고 각각의 챔버에 백색 LED를 설치 후 완전임의배치법으로 $20cm{\times}20cm$ 간격으로 재식하였다. 광도는 100, 200, $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$로 처리하였고, 광주기는 12/12, 18/6, 24/0(명기/암기)으로 처리하였다. 온도는 $21{\pm}2^{\circ}C$, 상대습도는 $60{\pm}10%$로 조절하여 22일간 재배하였다. 엽폭과 엽장, 생체중과 건물중, 총 안토시아닌 함량은 24/0(명기/암기) 처리에서 좋았다. 최대근장, 생체중과 건물중, 엽수는 100, $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$보다 $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ 처리에서 좋았다. 엽록소는 광주기 18/6과 24/0 처리 보다 12/12(명기/암기) 처리에서 높았다. 본 실험의 결과로 광도는 200, $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 광주기는 12/12 또는 18/6(명기/암기) 처리를 하는 것이 좋을 것으로 판단된다.