• 한국균학회지
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• 제27권1호통권88호
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• pp.73-75
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• 1999

Three species of the genus Xylaria are recognized on Taiwan materials: X. allantoidea (Berk.) Fr. and X. obovata (Berk.) Fr.; including one new record X. curta Fr., having $9.0-10.8{\times}3.9-5.4\;{\mu}m$ ascospores, in Taiwan. The collection of Xylaria allantoidea from Taiwan is compared to other collections in morphological characters. The species are described from the host and in culture by a bright microscope and a scanning electron microscope.

• Journal of the Korean Wood Science and Technology
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• 제38권6호
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• pp.579-586
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• 2010

목련과 식물의 활용도 제고를 위해 항산화제, 식품첨가제 개발에 필요한 항균 및 항산화 활성을 검정하였다. 우선 목련과 총 11종 식물의 수피, 잎, 목부, 꽃 및 열매의 에탄올 조추출물을 1,000 ${\mu}g/m{\ell}$로 조제하여 항진균 활성을 조사하였다. 그 결과 일본목련 수피가 백색부후균인 Trametes versicolor를 제외한 모든 공시균주에 대하여 50% 이상의 균사생장억제율을 나타내어 항균활성이 가장 높은 것으로 나타났다. 그 다음이 일본목련 목부로 탄저병균인 Glomerella cingulata와 목재부후균인 T. versicolor, Tyromyces palustris를 제외한 공시균주에 대하여 50% 이상의 균사생장억제율을 나타내었다. 수종별로는 일본목련이 가장 항균활성이 우수하여 수피, 잎, 목부 모두 항균활성이 있는 것으로 나타났으며 그 다음이 목련, 함박꽃나무 순이었다. 부위별로는 수피가 가장 활성이 우수하였으며 백목련, 자목련의 경우에는 다른 부위에 비해 꽃의 항균활성이 우수하였다. 또한, 프리라 디칼소거능을 측정하여 항산화활성을 검정한 결과에서는 100 ${\mu}g/m{\ell}$의 농도에서 태산목 수피와 잎이 70% 이상의 소거능을, 그리고 초령목 수피와 잎, 흑오미자 줄기 및 남오미자 줄기가 90% 이상의 소거능을 나타내었다. 특히, 흑오미자 줄기 및 남오미자 줄기는 10 ${\mu}g/m{\ell}$의 농도에서 각각 41.2%와 44.4%의 소거능을 나타내어 합성 항산화제인 butylated hydroxyanisole 및 천연항산화제인 ${\alpha}$-tocopherol과 동등한 항산화활성이 있는 것으로 밝혀졌다. 금후 흑오미자 및 남오미자 줄기와 같이 항산화활성 우수 시료는 활성물질 분리 및 구조 동정, 안전성 검정 등을 통하여 식품보존제 및 화장품 소재로 개발이 가능할 것으로 사료되었다.

• 대한약학회:학술대회논문집
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• 대한약학회 2000년도 NEW STRATEGY FOR DRUG DEVELOPMENT IN POST-GENOMIC ERA(대한약학회)
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• pp.244.2-244.2
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• 2000

• Natural Product Sciences
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• 제14권1호
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• pp.51-55
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• 2008

Three sesquiterpene-lignans, eudeshonokiol B (1), eudesobovatol B (2), and clovanemagnolol (3), were isolated from the stem bark of Magnolia obovata, together with magnolol (4), honokiol (5), and obovatol (6) on the basis of spectroscopic and physicochemical analyses including 2D NMR and Mass. Compounds 1 - 3 were belongs to a unique class of natural products made up of a sesquiterpene and biphenyl-type neolignan via an ether bond. All the isolated compounds were tested in vitro for their cytotoxic activity against the HeLa, A549, and HCTll6 cancer cell lines. Compounds 1 - 6 showed the cytotoxic activity against tested cancer cell lines, with $IC_{50}$ values ranging from 7.1 to 14.4 ${\mu}g/mL$.

• 생약학회지
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• 제32권2호통권125호
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• pp.168-174
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• 2001

Induction of phase II enzymes is a major mechanism of chemoprevention. The induction levels of quinone reductase (QR) activity in cultured murine hepatoma (Hepa 1c1c7) cells by 80%-methanol extracts of traditional medicinal plants were measured. Among the tested 81 plants, the extracts of Aralia continentalis, Magnolia obovata, and Viscum album were found to induce QR activities over 250%. The maximum induction levels obtained were 401.9%, 270.5%, and 301.8% by treatments of the extracts of A. continentalis $(318\;{\mu}g/ml)$, M. obovata $(53.8\;{\mu}g/ml)$ and V. album $(80.6\;{\mu}g/ml)$, respectively. These QR induction activities were more potent than those of the known QR inducers, t-butylhydroquinone (170.1%) and ${\beta}-naphthoflavone$ (320.0%).

• 조경수
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• 통권112호
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• pp.10-11
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• 2009

도입 수종이지만 우리나라에서 자라고 있는 나무 중 잎의 크기가 가장 크며 자라는 속도도 매우 빠르다. 특히나 지금까지 우리가 잘못알고 있는 후박피(厚朴皮) 즉 후박나무 껍질은 지금 소개드리는 중국목련과 일본목련의 걷껍질이 한방에서 사용되는 후박(厚朴)이다. 몇 년 전 경희대학교 한의학과 교수님이 중국에서 중국 후박나무 묘목 한그루를 가져다 주시면서 홍릉수목원에서 잘 키워보라며 놓고 간 중국목련이 어느덧 4m정도 자랐다.

• Natural Product Sciences
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• 제11권1호
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• pp.16-21
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• 2005

Nitric Oxide (NO), derived from L-arginine, is produced by two types (constitutive and inducible) of nitric oxide synthase (NOS: cNOS and iNOS). The NO produced in large amounts by the iNOS is known to be responsible for the vasodilation and hypotension observed in septic shock, cancer metastasis and inflammation. The inhibitors of iNOS, thus, may be useful candidates for the treatment of inflammatory diseases accompanied by the overproduction of NO. We prepared alcoholic extracts of herbal drugs which have been used for the treatment of inflammation in oriental medicine. We have screened the inhibitory activity of NO production in lipopolysaccharide (LPS)-activated macrophages after the treatment of these extracts. Among 82 kinds of extracts of herbal drugs, 35 extracts showed the potent inhibitory activity of NO production above 50% at the concentration of $50\;{\mu}g/mL$. The inhibitory activities of NO production were also evaluated for several solvent fractions at two different concentrations. Especially, hexane and EtOAc fractions of Alpinia officinarum, Angelica gigas, Ostericum koreanum, Saussurea lappa, Torilis japonica, and hexane fractions of Agrimonia pilosa, Machilus thunbergii, Hydrangea serrata, Magnolia obovata, Prunella vulgaris, Tussilago farfara, and EtOAC fractions of Perilla frutescence showed a significant activity at 10 and/or $25\;{\mu}g/mL$. In Western blot analysis, the hexane fractions ($5\;{\mu}g/mL$) of Magnolia obovata and Saussurea lappa, and EtOAc fractions ($20\;{\mu}g/mL$) of Hydrangea Serrata, Perilla frutescence and Torilis japonica inhibited the expression of iNOS protein in LPS-activated macrophages. These plants may be promising candidates for the study of the activity-guided purification of active compounds and might be useful for the treatment of inflammatory diseases and endotoxemia accompanying overproduction of NO.

• Journal of Applied Biological Chemistry
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• 제58권3호
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• pp.233-236
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• 2015

황목련 꽃으로부터 3개의 alkyl glycoside 화합물을 분리, 동정하였다. 황목련 꽃을 80% MeOH에 추출하여 이를 EtOAc, n-BuOH, 그리고 $H_2O$ 층으로 계통분획을 실시하였다. 이중 EtOAc 분획에 대하여 silica gel과 ODS column chromatography를 반복 실시하여 3종의 화합물을 분리, 정제하였다. Nuclear magnetic resonance, infrared spectroscopy 및 mass spectrometry의 spectroscopic data를 해석하여 이 화합물들을 2-methylbutan-1-ol-${\beta}$-$\small{D}$-galactopyranoside (1), 2-methylbutan-1-ol-${\beta}$-$\small{D}$-glucopyranoside (2), 2-methylpropan-1-ol-${\beta}$-$\small{D}$-glucopyranoside (3)로 각각 동정하였다. 동정된 화합물 모두 황목련 꽃에서는 이번 연구에서 처음으로 분리, 동정되었다.

• Journal of Microbiology and Biotechnology
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• 제25권9호
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• pp.1429-1432
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• 2015

To identify plant-derived cell signaling inhibitors with antifungal properties, a twocomponent screening system using both wild-type Cryptococcus neoformans and a calcineurin mutant was employed owing to their counter-regulatory actions on the Hog1 mitogenactivated protein kinase and calcineurin pathways. Of the 2,000 plant extracts evaluated, a single bioactive compound from M. obovata Thunb. was found to act specifically on the calcineurin pathway of C. neoformans. This compound was identified as magnoloside A, and had potent antifungal activities against various Cryptococcus strains with minimum inhibitory concentration values ranging from 1.0 to 4.0 μg/ml.

• 동의생리병리학회지
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• 제17권6호
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• pp.1528-1532
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• 2003

To investigate the antioxidant capacity of traditional Korean medicines, water extracts from 57 species were tested on their antioxidant activity using radical scavenging effects against ABTSㆍ+. Of which, Rhus javanica, Caesalpinia sappan, Rosa rugosa, Spatholobus suberectus, and Magnolia obovata showed strong antioxidant capacities at 10㎍ concentration. Therefore, antioxidant capacities of 5 traditional medicine extracts in the different concentration (1㎍, 5㎍, and 10㎍) were determined. The 5 traditional medicine extracts was detected in antioxidant capacity dose dependently. R. javanica was showed the highest antioxidant capacity, the antioxidant activity at 1 ㎍ of herbal extract being 0.85 mM TE. At the same time, the antiperoxidation effects of these 5 medicines were determined. Lipid peroxidation in brain homogenates induced by NADPH and ADP-Fe/sup 2+/ was strong inhibited by S, suberectus, R. rugosa, and M. obovata. Extract of C. sappan was showed the highest inhibition against lipid peroxidation compared with 4 herbal extracts in vitro. These traditional medicines are a potent antioxidant capacity and antiperoxidation activity, further investigation into the in vivo antioxidant therapeutic potential for treatment of human disorders such as Alzheimer's disease.