• Journal of Veterinary Clinics
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• v.18 no.2
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• pp.93-97
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• 2001

병원성 Mycoplasma hyopneumoniae strain 91-3, 비병원성 M. hyopneumoniae 그리고 M. flocculare를 돼지의 기관지섬모상피에 투여시 세포내 $Ca^{2+}$ 농도 [$Ca^{2+]}$$_{i}$ 의 변화를 본 연구에서 조사하였다. M. hyopneumoniae strain 91-3 (300-$\mu\textrm{g}$ml)를 투여시 기관지 섬모상피내의 $Ca^{2+}$가 투여전과 비교시 투여 후 250$\pm$19 nM (net increase)증가하였다 (10회 반복 47 cells). 이와는 대조적으로 비병원성 M. hyopneumoniae (300 $\mu\textrm{g}$ml) (6회 반복 18 cells)와 M. flocculare (300 $\mu\textrm{g}$ml) (8회 반복 24 cells)는 세포내 $Ca^{2+}$의 농도를 증가시키지 못하였다. 위의 결과로 병원성 M. hyopneumoniae 91-3 균주는 비병원성 mycoplasma와는 다르게 돼지의 섬모상피에서 [$Ca^{2+}$]$_{i}$ 을 유도하였으며 이러한 특성은 mycoplasma 감염증 치료에 중요한 단서를 제공할 뿐만 아니라 새로운 치료법의 개발에 유용한 스크리닝 기술에 응용될 것으로 기대된다.

• Korean Journal of Veterinary Research
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• v.42 no.2
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• pp.225-229
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• 2002

We report that mycoplasma organisms from lung tissues of slaughter pigs were identified to genes fragments with references use of nested-PCR technique(nPCR). Seven strains of mycoplasma species were isolated from 70 lung tissues. The organisms were detected by in vitro amplification of 16S rRNA and 23S rRNA genes. Nucleotide sequences of the spacer between 16S and 23S in the ribosomal RNA operons of mycoplasma were identified by the analysis of products from the nested PCR. Four common PCR primers, MhF1, MhF2 MhR1 and MhR2, were designed by analysis between these sequences by first amplified with F1, R1 and second with F2, R2, respectively. Specific amplification of the spacer region for reference strains of M. hyopneumoniae, M. hyorhinis, M. flocculare were confirmed by first round of PCR in which the traduced fragments of 690bp, 460bp, 630bp. But amplications of second round was changed to 240bp, 210bp, 230bp, respectively. Three different strains (M. hyopneumoniae:4, M. hyorhinis:2, M. flocculare:1) were detected by the nested-PCR technique. The results suggest that the detection of swine mycoplasma by n-PCR can be analyzed the nucleotide sequences between rRNA operons and homology study.