• Korean Journal of Food Science and Technology
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• v.35 no.6
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• pp.1117-1123
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• 2003

The effects of heat treatment on the physical and physical and physiological properties of wax gourd (Benincasa hispida) were examined. The applied heat treatments were autoclaved at $121^{\circ}C$ for 1 hr, boiled for 30 min, and microwaved at 680 W for 5 min. The water retention capacity (WRC) of the wax gourds was 9.43 g/g for the microwaved samples, 5.12 g/g for the boiled samples, 4.63 g/g for the raw samples, and 2.61 g/g for the autoclaved samples. Heat treatment caused to increase swelling by up to $4.4{\sim}7.8\;mL/g$. Calcium binding capacity of heat-treated wax gourd increased in the order of microwaved, boiled, raw, autoclaved samples. Scanning electron microscopy (SME) showed that autoclaving caused the most severe structural modifications, while microwave treatment produced the least modifications. The retarding effect on glucose and bile acid transport depended on the heat treatment. Only boiling showed the glucose retardation effect. Bile acid retardation effect increased in order of boiling (22.9%), autoclaving (17.1%), microwave treatment (14.3%), and raw wax gourd (8.6%). The cadmium retardation effect was significantly high in all samples.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2009.10a
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• pp.474-477
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• 2009

In this paper, flow stress of Al6061 is obtained by compression test in the range of temperature from $300^{\circ}C$ to $550^{\circ}C$ and effective strain-rate from 0.1/s to 20.0/s. The flow stress information is used to simulate an aluminum hot forging process. Non-isothermal simulation is carried out by a rigid-thermoviscoplastic finite element method. The predictions are compared with the experiments in terms of the deformed shape of material.

• Korean Journal of Materials Research
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• v.2 no.3
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• pp.220-227
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• 1992

Electrorheological(ER) fluid's storage shear modulus(G') and loss factor(${\eta}$) have been directly measured using small amplitude forced oscillating rheometer as a function of oscillating frequency, strain amplitude and applied electric field. Two types of experiment were performed , (a) frequency sweep and (b) amplitude sweep. Two kinds of sample were employed for this experiment ; cornstarch particles in corn oil and zeolite particles in silicone oil. The storage shear modulus was a strong function of driving frequency. Generally, the modulus increased with driving frequency. On the other hand, the loss factor was not well behaved as storage modulus, but as the driving frequency increases the loss factor slightly decreases was the trend of the material's characteristics. Also the modulus was a strong function of strain amplitude. Generally, modulus decreased with increasing strain, but loss factor increases slightly with increasing strain amplitude. For G', cornstarch in corn oil ER fluid has higher values than zeolite based fluid as we increased applied electric field. On the other hand, zeolite based fluid has higher values for ${\eta}$. There is a reasonable agreement between theoretical calculation and experiment.

• Korean Journal of Microbiology
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• v.40 no.2
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• pp.147-153
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• 2004

Protein modification by N-acetyl-${\beta}$-D-glucosamine (O-G1cNAc) on the hydroxyl groups of Ser or Thr ubiq-uitously occurs in eukaryotic cells and is involved in many cellular phenomena. The level of O-G1cNAc-mod-ified protein is regulated by OGT and O-GlcNAcase enzymes. We have tried to produce recombinant O-GlcNAcase in E. coli as an effort to establish in vitro screening system for modulators of O-GlcNAcase. The culture conditions for improvement of O-GlcNAcase productivity, were as follows: induction temperature, $30^{\circ}C$; the concentration of L-arabinose, 0.02% and induction time, 5 hr. Under these culture conditions, E. coli cells containing O-GlcNAcase gene had no enzyme activity until up to 3 hr culture. However, O-GlcNAcase activity dramatically increased from 3 to 5 hr culture. It almost maintained the same level after 5 hr culture. Western blot analysis verified the amount of expressed O-GlcNAcase increased with culture time, being con-sistent with activity data. The optimal reaction condition determined in this study was as follows: protein quan-tity, $5{\mu}g$; reaction time, 30 min; reaction temperature, $45^{\circ}C$; substrate concentration, 2 mM; reaction pH, 6.5. Methanol had little effect on O-GlcNAcase activity and 90% of activity were retained at 10%. Only 15% resid-ual activity were detected at 5% of chloroform.

• Korean Journal of Environmental Biology
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• v.39 no.4
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• pp.445-451
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• 2021

In this study, we described the production of an antibody to living modified organisms (LMOs) containing the gene encoding for 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) from Agrobacterium tumefaciens strain CP4 EPSPS provides resistance to the herbicide glyphosate (N- (phosphonomethyl) glycine). These LMOs were approved and have recently been used in the feed, food production, and processing industries in South Korea. Highly efficient monoclonal antibody (mAb) production is crucial for developing assays that enable the proper detection and quantification of the CP4 EPSPS protein in LMOs. This study describes the purification and characterization of recombinant CP4 EPSPS protein in E. coli BL21 (DE3) based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrixassisted laser desorption/ionization time-of-flight mass spectrometry. The production of mAbs was undertaken based on the standard operating procedure of Abclon, Inc.(South Korea), and the purity of the mAbs was assessed using SDS-PAGE. The following five mAb clones were produced: 2F2, 4B9, 6C11, 10A9, and 10G9. To verify the efficiency and specificity of the five developed mAbs, we performed Western blotting analysis using the LM (living modified) cotton crude extracts. All mAbs could detect the CP4 EPSPS protein in the LM cotton traits MON1445 and MON88913 with high specificity, but not in any other LM cottons or non-LM cottons. These data indicate that these five mAbs to CP4 EPSPS could be successfully used for the further development of antibody-based detection methods to target CP4 EPSPS protein in LMOs.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.35 no.4
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• pp.747-757
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• 2015

The main purpose of this study is to investigate the static behavior of a horizontally curved prestressed concrete (PSC) girder. A 30m long full-scale curved PSC girder with 80.0m radius is fabricated by a portable curved form system. Deflections and concrete strains at the middle of span were measured. The obtained experimental results have been compared to those from F.E.A. analysis. When a initial crack developed, the applied load was 1.3 times the service design load and the vertical deflection at the middle of span satisfied the requirement for a live load state according to the Korea Bridge Design Specifications (2010). Also, the ductility of the full scale specimen satisfied the limit in the Specifications (2010). To verify the experimental results, a numerical F.E. analysis was carried and confirmed that the data were similar with results from the test above. The horizontally curved PSC girder fabricated on site was found to have enough strength for safety under and after construction.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2008.10a
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• pp.95-98
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• 2008

In the present work, cross-roil rolling was rallied out using a rolling mill in which the roll axes are tilted by $5^{\circ},\;7.5^{\circ},\;10^{\circ}$ towards the transverse direction of the roiled sample. The evolution of strain states during cross-roll rolling was investigated by three-dimensional finite element method (FEM) simulation. Parallel to cross-roll rolling, normal-rolling using a conventional rolling mill was also carried out in the same rolling condition for clarifying the effect of cross-roll rolling. It turned out that three shear rate components were all introduced to the rolled sample by the cross-roll rolling process, while only one shear rate component operated during normal-rolling.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2009.10a
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• pp.427-428
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• 2009

In the manufacture process of refrigerator cabinet, polyurethane(PU) liquid firstly is injected between outer plate and inner case in high temperature about $40^{\circ}C$, and PU foam is generated and solidified to the room temperature. There will be great residual stress in the PU foam, especially at the corners after the whole refrigerator is completely assembled. The stress condition will become more complicated under operating condition because of the large temperature difference between the freezing room and outer plate. And also, there are great differences of properties for plastic and steel which would cause different deformation under temperature gradient. The steel outer plate would expand compared to the PU foam or ABS material under operation condition, which induces a thermally bowing deformation in the refrigerator cabinet. The objective of this paper is to design an optimum bead structure or to use reinforcement in the refrigerator except for improving material properties in order to decrease the deformation.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2009.05a
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• pp.306-309
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• 2009

The influence of equal-channel angular pressing (ECAP) route on dynamic deformation behavior of ultra-fine grained Al-4.4%Mg alloys was investigated in this study. The 8-pass ECAPed specimens consisted of ultra-fine grains of $0.5{\mu}m$ in size, and contained the considerable amount of second phase particles, which were fragmented and distributed homogeneously in the matrix. The result of dynamic torsional tests indicated that the maximum shear stress and fracture shear strain were lowest in the specimen deformed by ECAP via route A among the 8-pass ECAPed specimens. The formation of adiabatic shear bands was addressed by concepts of critical shear strain, deformation energy required for void initiation, and microstructural homogeneity related to ECAP routes.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2007.10a
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• pp.352-355
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• 2007

Polycrystalline materials such as steels(BCC) and aluminum alloys(FCC) show the strain hardening and the strain rate hardening during the plastic deformation. The strain hardening is induced by deformation resistance of dislocation glide on some crystallographic systems and increase of the dislocation density on grain boundaries or inner grain. However, the phenomenon of the strain rate hardening is not demonstrated distinctly. In this paper, tensile tests for various strain rates are performed in the rage of $10^{-2}$ to $10^2s^{-1}$ then, specimens are extracted on the same strain position to investigate the microscopic behavior of deformed materials. The extracted specimen is investigated by using the electron backscattered diffraction(EBSD) and transmission electron microscopy(TEM) results which contain grain size, grain shape, aspect ratio and dislocation substructure.