• Journal of Korean Biological Nursing Science
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• 제10권2호
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• pp.176-183
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• 2008

Purpose: The purpose of this study was to identify the effect of Environmental Enrichment (EE) on improvement of motor function in animal models of Parkinson's Disease. Methods: Male C57BL6 mice weighing 25-30 g, at the age of 12 wks were used in this study. The animals were injected MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridin, 20 mg/kg in saline, i.p.) 4 times a day at every 2 hr, and raised in EE cage for 14 days. On day 14, after behavior test, all mice were sacrificed for immunohistochemistry. All values were expressed as means$\pm$S.E.M. Statistical significance was evaluated using a one way ANOVA followed by Sheffe test. Results: There was a significant difference between the experimental group and the control group in the behavior test. Also EE significantly reduced of TH positive cell loss in Substantia nigra pars compacta as compared to the result of MPTP treatment. Conclusion: Based on these findings, it is reasonable to assume that the environmental enrichment prevents dopaminergic neuronal loss and improves disarrangement of motor function and behavioral disability induced by MPTP.

• 혜화의학회지
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• 제29권1호
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• pp.1-17
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• 2020

Objectives : The objectives of this study is to develop a mouse model similar to Taeeum-type by inducing Lung fibrosis with bleomycin, and to determine adequate concentration of bleomycin. Methods : The subjects were divided into six groups: normal, obesity induced group, and bleomycin administered 0.015U, 0.03U, 0.06U, and 0.09U(U/100g bw) concentrations respectively. Each concentration of bleomycin was dissolved in distilled water, and administered through Intra-Nazal-Trachea injection method. Food intake and body weight were measured at regular time weekly. At the end of the experiment, blood was gathered by cardiac puncture for biochemical examinations, organs were removed for histological examinations, and weigh and mRNA genes was analyzed. Result : Mice administered with bleomycin at 0.015U and 0.03U showed body and fat weight gain, and increased blood total cholesterol, LDL-cholesterol, glucose, and free fatty acid level. Fat related genes also showed higher level than the control group. Obesity was most strongly induced in the mice administered with 0.03U of bleomycin. On the other hand, when bleomycin was administered at concentrations above 0.06U, a model of obesity mouse was not created due to rapid emphysema inflammation and weakness. Conclusions : Mice were most vulnerable to obesity when bleomycin was administered at a concentration of 0.3 to cause liver damage. Bleomycin concentration over 0.06U did not cause obesity-induced mice, due to severe damage in liver.

• 생명과학회지
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• 제17권1호
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• pp.143-149
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• 2007

Scutellaria baicalensis GEORGI(SB) is used in oriental medicine for the treatment of incipient strokes. Although it has been reported that SB is neuroprotective in a hypoxia model, its mechanism is poorly understood. Here, we investigated the effect of SB on the modulation of retinoic acid receptor a (RARa). Rat cerebrocortical cells were grown in neurobasal medium. On DIV12 cells were treated with SB $(20{\mu}g/ml)$ and given a hypoxic shock $(2%\;O_2/5%\;CO_2,\;3hr)$ on DIV14. In situ hybridization using cRNA probe revealed that RARa mRNA punctae are distributed, in addition to nucleus, throughout neuronal dendrites, where SB upregulated its density by 69.8% (p=0.001) and 129.8% (p=0.001) in both normoxia and hypoxia, respectively. At the protein level, SB upregulated RARa in the neuronal soma by 78.8% (p=0.004) and 23.6% (p=0.001) in both normoxia and hypoxia, respectively. These results indicate that SB upregulates RARa in both normoxia and hypoxia, which might contribute to the neuroprotection.

• 태양에너지
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• 제10권2호
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• pp.10-17
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• 1990

건물의 사양으로부터 시뮬레이션에 의해 열성능을 추출하는 기법과 실측 데이타로 부터 연역적인 방법에 의해 열성능을 추출하는 기법을 연합시켜 단기간의 측정에 의해 건물의 열성능을 간단하고 정확하게 결정할 수 있는 기법을 Test cell에 적용하였다. 실험과정 및 자료분석은 PSTAR 기법에 따라 수행했다. 각 측정기간은 3일간으로 비거주 상태에서 측정했으며, 실내온도, 외기온, 일사량, 공급열량, 풍속, 상대습도등의 자료를 측정하여 건물의 사양에 의한 시뮬레이션 모델의 재표준화에 사용했다. 아래의 3개 주요계수들이 본 분석에 의해 얻어졌다. 1) 건물 열손실계수 : BLC(표면전도율+침기에 의한 전도율); 2) 유효 건물열용량; 3) 유효 태양열 획득량. 그리고 재표준화된 식으로부터 예측된 값과 건물의 사양으로 시뮬레이션에 의해 예측된 값과 측정된 값과의 비교를 통해 재표준화된 식이 실제건물의 열성능을 잘 묘사함을 알 수 있었다. 장기간의 외삽으로 기후 자료를 이용하여 표준 쾌적도를 유지하기 위해 필요한 에너지를 계산했다.

• 대한한방내과학회지
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• 제40권4호
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• pp.567-581
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• 2019

Objective: This study aimed to evaluate the inhibitory effects of SGX01 on the lung injuries of COPD mice model. Materials and Methods: This study was carried out in two ways: in vitro and in vivo. In vitro, L929 cells were challenged with LPS, and then treated with six concentrations of SGX01 (10, 30, 50, 100, 300, and $500{\mu}g/ml$) and analyzed by ELISA. In vivo, C57BL/6 mice were challenged with LPS and cigarette smoking solution (CSS), and then treated with a vehicle only (control group), dexamethasone 3 mg/kg (dexa group), or a SGX01 200 mg/kg (SGX01 group). After sacrifice, the BALF or lung tissue was analyzed with Cytospin, FACS, ELISA, real-time PCR and H&E, and Masson's trichrome staining. Results: SGX01 significantly decreased NO, $TNF-{\alpha}$, and IL-6 on L929 cells challenged with LPS. In the COPD model, SGX01 significantly inhibited the increase of neutrophils, $TNF-{\alpha}$, IL-17A, CXCL-1, MIP2, CD8+ cells in BALF, and $TNF-{\alpha}$, $IL-1{\beta}$ mRNA expression in lung tissue. It also decreased the severity of the histological lung injury. Conclusion: This study suggests the usability of SGX01 for COPD patients by controlling lung tissue injury.

• 대한본초학회지
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• 제24권1호
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• pp.73-78
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• 2009

Objectives : In this study, we studied the effect of Pinellia Ternata (PT) on regulatory T cells and CD3+CCR3+ Th2 cells number in asthma model mice. Methods : All mice were immunized on two different days (21 days and 7 days before inhalational exposure) by i.p. injections of 0.2 $m\ell$ alum-precipitated Ag containing 100 ${\mu}g$ of OVA bound to 4 mg of aluminum hydroxide in PBS. Seven days after the second sensitization, mice were exposed to aerosolized ovalbumin for 30 min/day on 3 days/week for 12 weeks(at a flow rate of 250 L/min, 2.5％ ovalbumin in normal saline) and PT (400, 200 mg/kg) were orally administered 3 times a week for 8 weeks. After C57BL/6 mice were orally given of PT, the percentages, cell numbers, phenotype and function of CD4+CD25+Treg cells were determined by flow cytometry. Results : The cell numbers of CD4+CD25+Treg cell subsets were markedly increased in PT treated mice as reported. However, PT significantly reduced the CD3+CCR3+ Th2 cells in PBMC and lung of mice. Conclusions : These results indicate that PT has a deep inhibitory effect on asthma model mice by increase the number of regulatory T cells, and by reducing CD3+CCR3+ Th2 cells.

• Nuclear Engineering and Technology
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• 제26권4호
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• pp.516-525
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• 1994

TMI 및 Chernobyl 사고이후 향후 원전에 대한 안전성 향상을 강화하기위해 개량형 원전에 대해 여러가지 피동형 안전설비가 제안되고 있다. 피동냉각계통의 타당성을 검증하고 상세 설계자료를 제공하기 위해, 본 연구는 웨스팅하우스사의 AP-600 피동격납용기와 같은 한쪽 가열면을 갖는 폐쇄유로에 대한 공기 유입구 위치 및 외부영향이 자연순환 공기유량에 미치는 영향과 자연 및 강제대류하에서 대류열전달계수를 조사하였다. 본 실험은 AP-600 격납용기를 1/26로 축소한 segment 유형의 실험장치를 토대로 수행되었다. 자연 및 강제대류 조건하의 공기유로내 특정 위치에서 공기의 속도 및 온도를 측정하였다. 실험결과는 일차원 단순 모델과 비교하였으며, 좋은 일치점을 보였다.

• 한방안이비인후피부과학회지
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• 제19권2호
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• pp.1-18
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• 2006

Objective : Although management of asthma has become increasingly effective, its cure remains elusive, necessitating a new modality to prevent or eliminate causes triggering clinical progress. Based in the clinical experiences, a novel decoration Cheongsangbiyeum (CSB), has been developed to treat asthma, which consists of Polyporus, Semen Myristicae, Pericarpium citri Reticulatae, Rhizoma Cimicifugae, Cortex Albizziae, Fructus Rubi, Rhizoma Zedoariae, and Rhizoma Rhei. In the current study, its anti-asthmatic efficacy was evaluated using a mouse model of asthma. Methods : Experimental allergic asthma was induced by repeated intraperitioneal sensitization and intranasal challenge of ovalbumin (OVA). Water extract of CSB (1 mg/mouse/day) was administrated orally whereas control mice on given with identical volume of phosphate-buffered saline (PBS) for 5 days during the course of antigen challenge. When airway hyperreactivity(AHR) measured by ${\bata}-methacoline-induced$ airflow obstruction was compared, AHR of CSB-treated mice was significantly lower than those of control mice, indicating that CM extract can attenuate an asthmatic symptom. Airway recruitment of leukocytes and eosinophils was also markedly reduced by CSB treatment suggesting that oral treatment of CSB can alleviate the airway inflammation. For a better understanding of possible mechanisms underlying anti-asthmatic effet of CSB, cytokine (IL-4, IL-5, IL-13 and $IFN{\gamma}$ levels in bronchoalveola lavage fluid (BALF) and lung tissues were determined. Results : The results showed that cytokine levels were significantly lowered by CSB treatment. Additionally, number of draining lymph node cells was significantly lower than those of control mice. These data indicate that CSB suppress in vivo allergen-specific response. However, notably, levels of type 2 cytokines such as IL-5 and IL-13 were more profoundly influenced. Moreover, in vitro OVA-specific proliferative response and type 2 cytokine (IL-4, IL-5 and IL-13) production lymph node cells was markedly decreased in CSB-treated mice, whereas their $IFN{\gamma}$ production was not significantly altered Thrse data clearly showed a preferential inhibition of type 2 T cell (Th2) response by CSB treatment. This finding was also supported by serum antibody data showing that levels of OVA-specific type 2 antibodies, IgE and IgG1, in CSB-treated mice were significantly lower than in control mice, while type 1 antibody, IgG2a level m rather higher than controls, although the difference was in significant. Conclusions : In conclusion, oral administration of CSB attenuates asthmatic manifestations including AHR ad airway recruitment of eosinophils in a mouse model which possibly results from selective inhibition of Th2 cell response to allergen. Our data suggest a potential clinical application of CSB for control of allergic asthma.

• 생물환경조절학회지
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• 제32권1호
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• pp.34-39
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• 2023

식물공장에서 생육 모델링은 안정적인 생산과 수확량을 조절하는 데 필수적일 뿐만 아니라 환경 데이터와 생육량의 관계를 비교하여 환경 조건을 제어할 수 있는 도구가 되기도 한다. 본 연구는 식물공장에서 재배하는 들깨[Perilla frutescens (L.) Britt.]에 대해 선형지수함수를 이용해 모델링하였다. 광도, 광주기, 혼합광의 비율을 각각 130μmol·m^-2·s^-1, 12/12시간, R:G:B(7:1:2)로 설정하여 정식한 후 화아분화가 발생할 때까지 12회 생육 조사하였고, 건물중과 생체중 예측을 위해 선형지수함수를 이용하여 모델링하였다. 생체중과 건물중 간의 상관계수는 가장 높은 양의 상관관계(r = 0.996)를 보였고, 생체중과의 관계에서 건물중을 제외하면 엽면적, 초장, 엽수, 마디수, 엽장, 엽폭의 순으로 높은 양의 상관관계를 나타내었다. 생육 예측을 위한 독립변수로 정식 후 일수, 엽면적과 초장을 사용하였는데, 생육 예측을 위한 적합한 독립변수로는 엽면적이었다. 다만, 생육을 예측하기 위한 파괴적인 방법 또는 비파괴적인 방법에 대해 고려해야 할 것이다. 본 연구에서 식물공장에서 들깨의 생육을 예측하기 위한 생육 모델식을 만들었다.

• 한국식품위생안전성학회지
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• 제36권6호
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• pp.528-536
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• 2021

본 연구는 십자화과 채소인 갓(Brassica juncea) 추출물을 이용하여 지표성분인 sinigrin의 함량을 분석하고, 내분비계 교란물질 환경호르몬인 비스페놀 A (BPA)로 분화를 유도한 3T3-L1 전구지방세포에서 갓 추출물과 sinigrin 처리에 대한 지방세포 분화 및 활성산소종(ROS) 생성 억제, 지방 생성 전사인자(PPARγ, C/EBPα, aP2)의 단백질 발현 감소 효능을 평가하였다. 연구 결과에 따르면 HPLC를 이용하여 측정한 갓 추출물 중 sinigrin의 함량은 21.27±0.2 mg/g 인 것으로 나타났다. BPA로 유도된 3T3-L1 전구지방세포에서 XTT assay 결과 sinigrin 180 µM 및 갓 추출물 300 ㎍/mL 농도에서 세포 독성을 보이지 않았으며, 지방세포 분화과정 중 세포 내 지방 축적량과 ROS 생성량을 비교하였을 때 갓과 sinigrin을 처리한 지방세포의 경우 지방축적량 및 ROS 생성량 모두 유의적으로 감소시키는 것으로 나타났다. 또한, 갓 추출물 및 sinigrin을 처리하였을 때 지방세포 분화를 조절하는 전사인자 PPARγ, C/EBPα 및 aP2의 발현이 억제됨을 확인하였다. 이 결과를 통해 갓은 환경호르몬 비스페놀 A로 유도된 지방세포 내 지방 축적 억제와 더불어 ROS 저감에 효과적으로 작용함을 확인하였다. 향후 sinigrin을 함유한 갓은 BPA로 인한 지질 대사 장애를 예방하는 천연물 유래 기능성 식품 소재로 활용할 가능성이 높은 것으로 기대되며, 추가로 임상 연구 및 작용기전 입증을 위한 in vivo 모델에서의 후속 연구가 진행되어야 할 것으로 사료된다.