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Genetic Diversity and Association Analyses of Chinese Maize Inbred Lines Using SSR Markers

  • Vathana, Yin;Sa, Kyu Jin;Lim, Su Eun;Lee, Ju Kyong
    • Plant Breeding and Biotechnology
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    • v.7 no.3
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    • pp.186-199
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    • 2019
  • We selected 68 Chinese maize inbred lines to understand the genetic diversity, population structure, and marker-trait associations for eight agronomic traits and 50 simple sequence repeats (SSRs) markers. In this study, effective traits, such as days of anthesis (DA), days of silking (DS), ear height (EH), plant to ear height ratio (ER), plant height (PH), and leaf width (LW) were divided into PC1 and PC2 by PCA analysis for maize inbred lines. Genetic diversity analysis revealed a total of 506 alleles at 50 SSR loci. The mean number of alleles per locus was 10.12. The averages of genetic diversity (GD) and polymorphic information content (PIC) values were 0.771 and 0.743, respectively. Based on a membership probability threshold of 0.80, the population structure revealed that the total inbred lines were divided into three major groups with one admixed group. A marker-trait association using Q + K MLM showed that nine SSR markers (bnlg1017, umc2041, umc2400, bnlg105, umc1229, umc1250, umc1066, umc2092, and umc1426) were related with seven agronomic traits. Among these SSR markers, eight SSR markers were associated with only one agronomic trait (DA, DS, ER, LL, LW, PH, and ST), whereas one SSR marker (umc1229) was associated with two agronomic traits (DA and ST). These results will help in optimizing the choice of inbred lines for cross combinations, as well as in selecting markers for further maize breeding programs.

Soil Moisture Estimation and Drought Assessment at the Spatio-Temporal Scales using Remotely Sensed Data: (I) Soil Moisture (원격탐사자료를 이용한 시⋅공간적으로 분포되어 있는 토양수분산정 및 가뭄평가:(I) 토양수분)

  • Shin, Yongchul;Choi, Kyung-Sook;Jung, Younghun;Yang, Jae E.;Lim, Kyoung-Jae
    • Journal of Korean Society on Water Environment
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    • v.32 no.1
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    • pp.60-69
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    • 2016
  • In this study, we estimated root zone soil moisture dynamics using remotely sensed (RS) data. A soil moisture data assimilation scheme was used to derive the soil and root parameters from MODerate resolution Imaging Spectroradiometer (MODIS) data. Based on the estimated soil/root parameters and weather forcings, soil moisture dynamics were simulated at spatio-temporal scales based on a hydrological model. For calibration/validation, the Little Washita (LW13) in Oklahoma and Chungmi-cheon/Seolma-cheon sites were selected. The derived water retention curves matched the observations at LW 13. Also, the simulated soil moisture dynamics at these sites was in agreement with the Time Domain Reflectrometry (TDR)-based measurements. To test the applicability of this approach at ungauged regions, the soil/root parameters at the pixel where the Seolma-cheon site is located were derived from the calibrated MODIS-based (Chungmi-cheon) soil moisture data. Then, the simulated soil moisture was validated using the measurements at the Seolma-cheon site. The results were slightly overestimated compared to the measurements, but these findings support the applicability of this proposed approach in ungauged regions with predictable uncertainties. These findings showed the potential of this approach in Korea. Thus, this proposed approach can be used to assess root zone soil moisture dynamics at spatio-temporal scales across Korea, which comprises mountainous regions with dense forest.

Safety analysis for the tunnel adjacent to the pier (교각에 근접한 터널의 안정성 평가)

  • Lee, Sun-Bok;Yoon, Ji-Son
    • Journal of the Korean Geophysical Society
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    • v.7 no.4
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    • pp.313-324
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    • 2004
  • The finite element method and statistics of the convergence measurement are useful method of the stability analysis of the tunnel adjacent to the pier. It is the purpose of the this case study to certificate of validity of the application of those methods. The safety of the pilot tunnel method and LW pre-grouting has been evaluated from the FEM analysis. The three-dimensional finite element method is carried out for the decision of the level of stress redistribution at the two-dimensional numerical analysis. An analysis of the convergence is carried out by the estimation of preceding convergence at tunnel excavation. F-examination is applied for this estimation. As results of that analysis, The F-value is from 10.81 to 158.74 and the coefficient of determination is from 0.82 to 0.99. An analysis of convergence is carried out by using regression analysis. Consequently, it is shown that the convergence can be modeled as following function C(t) = a[1-exp(-bt)].

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Relationship among porcine lncRNA TCONS_00010987, miR-323, and leptin receptor based on dual luciferase reporter gene assays and expression patterns

  • Ding, Yueyun;Qian, Li;Wang, Li;Wu, Chaodong;Li, DengTao;Zhang, Xiaodong;Yin, Zongjun;Wang, Yuanlang;Zhang, Wei;Wu, Xudong;Ding, Jian;Yang, Min;Zhang, Liang;Shang, Jinnan;Wang, Chonglong;Gao, Yafei
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.2
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    • pp.219-229
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    • 2020
  • Objective: Considering the physiological and clinical importance of leptin receptor (LEPR) in regulating obesity and the fact that porcine LEPR expression is not known to be controlled by lncRNAs and miRNAs, we aim to characterize this gene as a potential target of SSC-miR-323 and the lncRNA TCONS_00010987. Methods: Bioinformatics analyses revealed that lncRNA TCONS_00010987 and LEPR have SSC-miR-323-binding sites and that LEPR might be a target of lncRNA TCONS_00010987 based on cis prediction. Wild-type and mutant TCONS_00010987-target sequence fragments and wild-type and mutant LEPR 3'-UTR fragments were generated and cloned into pmiRRB-REPORTTM-Control vectors to construct respective recombinant plasmids. HEK293T cells were co-transfected with the SSC-miR-323 mimics or a negative control with constructs harboring the corresponding binding sites and relative luciferase activities were determined. Tissue expression patterns of lncRNA TCONS_00010987, SSC-miR-323, and LEPR in Anqing six-end-white (AQ, the obese breed) and Large White (LW, the lean breed) pigs were detected by real-time quantitative polymerase chain reaction; backfat expression of LEPR protein was detected by western blotting. Results: Target gene fragments were successfully cloned, and the four recombinant vectors were constructed. Compared to the negative control, SSC-miR-323 mimics significantly inhibited luciferase activity from the wild-type TCONS_00010987-target sequence and wild-type LEPR-3'-UTR (p<0.01 for both) but not from the mutant TCONS_00010987-target sequence and mutant LEPR-3'-UTR (p>0.05 for both). Backfat expression levels of TCONS_00010987 and LEPR in AQ pigs were significantly higher than those in LW pigs (p<0.01), whereas levels of SSC-miR-323 in AQ pigs were significantly lower than those in LW pigs (p<0.05). LEPR protein levels in the backfat tissues of AQ pigs were markedly higher than those in LW pigs (p<0.01). Conclusion: LEPR is a potential target of SSC-miR-323, and TCONS_00010987 might act as a sponge for SSC-miR-323 to regulate LEPR expression.

Performance, Carcass Quality and Fatty Acid Profile of Crossbred Wagyu Beef Steers Receiving Palm and/or Linseed Oil

  • Suksombat, Wisitiporn;Meeprom, Chayapol;Mirattanaphrai, Rattakorn
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.10
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    • pp.1432-1442
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    • 2016
  • The objective of this study was to determine the effect of palm and/or linseed oil (LSO) supplementation on carcass quality, sensory evaluation and fatty acid profile of beef from crossbred Wagyu beef steers. Twenty four fattening Wagyu crossbred beef steers (50% Wagyu), averaging $640{\pm}18kg$ live weight (LW) and approximately 30 mo old, were stratified and randomly assigned in completely randomized design into 3 treatment groups. All steers were fed approximately 7 kg/d of 14% crude protein concentrate with ad libitum rice straw and had free access to clean water and were individually housed in a free-stall unit. The treatments were i) control concentrate plus 200 g/d of palm oil; ii) control concentrate plus 100 g/d of palm oil and 100 g/d of LSO, iii) control concentrate plus 200 g/d of LSO. This present study demonstrated that supplementation of LSO rich in C18:3n-3 did not influence feed intakes, LW changes, carcass and muscle characteristics, sensory and physical properties. LSO increased C18:3n-3, C22:6n-3, and n-3 polyunsaturated fatty acids (PUFA), however, it decreased C18:1t-11, C18:2n-6, cis-9, trans-11, and trans-10,cis-12 conjugated linoleic acids, n-6 PUFA and n-6:n-3 ratio in Longissimus dorsi and Semimembranosus muscles.

Development of Landsat-based Downscaling Algorithm for SMAP Soil Moisture Footprints (SMAP 토양수분을 위한 Landsat 기반 상세화 기법 개발)

  • Lee, Taehwa;Kim, Sangwoo;Shin, Yongchul
    • Journal of The Korean Society of Agricultural Engineers
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    • v.60 no.4
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    • pp.49-54
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    • 2018
  • With increasing satellite-based RS(Remotely Sensed) techniques, RS soil moisture footprints have been providing for various purposes at the spatio-temporal scales in hydrology, agriculture, etc. However, their coarse resolutions still limit the applicability of RS soil moisture to field regions. To overcome these drawbacks, the LDA(Landsat-based Downscaling Algorithm) was developed to downscale RS soil moisture footprints from the coarse- to finer-scales. LDA estimates Landsat-based soil moisture($30m{\times}30m$) values in a spatial domain, and then the weighting values based on the Landsat-based soil moisture estimates were derived at the finer-scale. Then, the coarse-scale RS soil moisture footprints can be downscaled based on the derived weighting values. The LW21(Little Washita) site in Oklahoma(USA) was selected to validate the LDA scheme. In-situ soil moisture data measured at the multiple sampling locations that can reprent the airborne sensing ESTAR(Electronically Scanned Thinned Array Radiometer, $800m{\times}800m$) scale were available at the LW21 site. LDA downscaled the ESTAR soil moisture products, and the downscaled values were validated with the in-situ measurements. The soil moisture values downscaled from ESTAR were identified well with the in-situ measurements, although uncertainties exist. Furthermore, the SMAP(Soil Moisture Active & Passive, $9km{\times}9km$) soil moisture products were downscaled by the LDA. Although the validation works have limitations at the SMAP scale, the downscaled soil moisture values can represent the land surface condition. Thus, the LDA scheme can downscale RS soil moisture products with easy application and be helpful for efficient water management plans in hydrology, agriculture, environment, etc. at field regions.

Grouting Properties using Thixotropic Material and Vibration Impact Method (가소성 그라우트 재료와 진동 및 충격을 부여하는 공법에 의한 지반개량 특성)

  • Keeseok Kim;Haseog Kim;Bong-hyun Baek;Simhun Yuk
    • Resources Recycling
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    • v.33 no.1
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    • pp.37-47
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    • 2024
  • In grouting, the LW method is commonly employed to induce the gelation of cementitious material using water glass, thereby restricting the extent of material injection. Nevertheless, challenges manifest when materials are lost before gelation, particularly in regions with high groundwater flow rates or significant subsurface voids. This study developed a thixotropic grout material using LFS and GGBFS to mitigate material loss during injection, with an assessment of its flow characteristics, durability in marine exposure, strength, and injection properties. The outcomes revealed that the thixotropic grout material exhibited flow ranging from 105 to 143 mm and enhanced strength and durability compared to the LW method. Furthermore, field tests substantiated that applying vibration and impact improved impermeability.

Evaluation of Factors Related to Productivity and Yield Estimation Based on Growth Characteristics and Growing Degree Days in Highland Kimchi Cabbage (고랭지배추 생산성 관련요인 평가 및 생육량과 생육도일에 의한 수량예측)

  • Kim, Ki-Deog;Suh, Jong-Taek;Lee, Jong-Nam;Yoo, Dong-Lim;Kwon, Min;Hong, Soon-Choon
    • Horticultural Science & Technology
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    • v.33 no.6
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    • pp.911-922
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    • 2015
  • This study was carried out to evaluate growth characteristics of Kimchi cabbage cultivated in various highland areas, and to create a predicting model for the production of highland Kimchi cabbage based on the growth parameters and climatic elements. Regression model for the estimation of head weight was designed with non-destructive measured growth variables (NDGV) such as leaf length (LL), leaf width (LW), head height (HH), head width (HW), and growing degree days (GDD), which was $y=6897.5-3.57{\times}GDD-136{\times}LW+116{\times}PH+155{\times}HH-423{\times}HW+0.28{\times}HH{\times}HW{\times}HW$, ($r^2=0.989$), and was improved by using compensation terms such as the ratio (LW estimated with GDD/measured LW ), leaf growth rate by soil moisture, and relative growth rate of leaf during drought period. In addition, we proposed Excel spreadsheet model for simulation of yield prediction of highland Kimchi cabbage. This Excel spreadsheet was composed four different sheets; growth data sheet measured at famer's field, daily average temperature data sheet for calculating GDD, soil moisture content data sheet for evaluating the soil water effect on leaf growth, and equation sheet for simulating the estimation of production. This Excel spreadsheet model can be practically used for predicting the production of highland Kimchi cabbage, which was calculated by (acreage of cultivation) ${\times}$ (number of plants) ${\times}$ (head weight estimated with growth variables and GDD) ${\times}$ (compensation terms derived relationship of GDD and growth by soil moisture) ${\times}$ (marketable head rate).

Calibration and Validation of the Estimated Chlorophyll a Derived from KOMPSAT/OSMI Data and Fisheries Application in the East China Sea

  • Suh Young-Sang
    • Journal of Environmental Science International
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    • v.14 no.10
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    • pp.911-917
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    • 2005
  • A comparison between the estimated chlorophyll a from OSMI, the SeaWiFS and the chlorophyll a measured from the research cruises of National Fisheries Research and Development Institute was made. The updated empirical algorithm for calibrating and validating of the estimated chlorophyll a in the East China Sea was formulated by relationship between the estimated chlorophyll a and the field one. The relationship between the chlorophyll a and the band ratio(nLw490/555) was still highest in the OSMI data after launching of KOMPSAT satellite. The distributions of OSMI chlorophyll a were compared with those of sea surface temperature, zooplankton biomass, and catch amounts of the Pacific mackerel in the East China Sea. In case of the relationships in specially winter seasons of 2002 and 2004, the zooplankton and the fish were totally depended on the distributions of SST than those of chlorophyll a.

TOLAPS - A PROGRAM FOR TAKEOFF AND LANDING PROFILE SIMULATIN

  • Kare H. Liasjo;Herold Olsen;Idar L.N. Granoien;Hans E. Bohn
    • Proceedings of the Acoustical Society of Korea Conference
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    • 1994.06a
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    • pp.710-715
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    • 1994
  • The program name TOLAPS is an acronym for Take-Off LAnding Profile Simulation. Some of the interesting features of this program is the ability to detect flight performance effects of airport altitude, ambient temperature, air pressure and wind. TOLAPS can also handle effects of TOW and LW. The program user can also calculate profiles by user difined flaps and thrust settings deviating from recommended standard settings for each aircraft. Wind effects on straight out flying as well as turns can also be demonstrated. Output form TOLAPS are either screen graphics of profiles (altitude, speed or thrust versus flight distance) or flight track. Profiles can also be made in a tabular form, ready for use in most airport noise calculation programs. In this way, TOLAPS is a valuable tool to evaluate effects of noise abatement procedures.

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