• BMB Reports
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• v.29 no.6
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• pp.500-506
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• 1996

We have isolated a water-extracted novel regulator for blood coagulation from an earthworm, Lumbricus rubellus. As a folk remedy, the earthworm has been known to facilitate blood circulation. After complete heat inactivation of endogenous proteases in the earthworm, an anticoagulant(s) was purified through ammonium sulfate fractionation and three consecutive gel permeation chromatography of Sephacryl S-300, Sephadex G-75, and G-150 by measuring activated partial thromboplastin time (APTT) The anticoagulant was further purified to 2,800 fold with a C4 reversed-phase HPLC This activity was stable under heat ($100^{\circ}C$ for 30 min) and acidic conditions (0.4 N HCl). The effects of this partially purified anticoagulant on thrombin were observed with various substrates such as N${\alpha}$-benzoyl-DL-arginine-p-nitroanilide (BApNA), H-D-phenylalanyl-L-pipecoyl-L-arginine-p-nitroanilide (S-2238), N${\alpha}$-p-tosyl-L-arginine methyl ester (TAME), and fibrinogen as a natural substrate. Only TAME hydrolysis, due to an esterase activity of the enzyme, was inhibited among the chromogenic substrates. In addition, the anticoagulant not only inhibited the conversion of fibrinogen to fibrin but also prolonged the fibrin clot formation monitored with the in vitro coagulation test. Based on these observations, we suggest the significance of measuring the ability of antithrombotic drugs to inhibit the esterase activity of thrombin. In this report, it was also shown that the earthworm indeed contained a water-extractable, heat- and acid-stable anticoagulant which could be used as a novel antithrombotic agent.

• Journal of environmental and Sanitary engineering
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• v.15 no.3
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• pp.44-49
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• 2000

The purpose of this research is to evaluate the adsorption capacity of casts for heavy metals. The casts were excreted by earthworm, Lumbricus rubellus, after having eaten the paper sludge. Various batch experiments on adsorption were performed to compare cast and activated carbon. The pH increase in solution due to extractives from cast was 1.3 and the cation exchange capacity which implies adsorption capacity for solubles is greater on activated carbon than on cast. According to the results of batch experiment, the removal rates of Pb, Cu, Cr, Zn using the activated carbon and casts as adsorbent were 98%, 93%, 94%, 89%, 82% and 95%, 90%, 88%,80%, 66%, respectivity, and this removal were achived less than 90 minutes. It can be said that casts is so good adsorbent as activated carbon is, although adsorption carbon was found to be some large than those of casts through Freundlich isotherm applied for adsorption of soluble. As a result on the experiment of isothermic adsorption from the mixed component solution in the batch, the order of preferable elements in heavy metal adsorption was found to be Pb＞Cd＞Cu＞Cr＞Zn on activated carbon, respectively.

• The Korean Journal of Ecology
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• v.14 no.1
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• pp.113-117
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• 1991

Comparisons of the physico-chemical properties of earthworm(Lumbricus terrestris) soil casts and top soil of red pine forests in a limestone area were carried out. The casts production durign August and September in 1990 amounted to 6∼7t/ha. The particle of top soil and casts ranged 40∼50% and 10∼20% for sand , 25∼30% and 30∼35% for silt, and 20∼25% and 55∼65% clay, respectively. Significant difference in pH value was not observed between casts and top soil. The casts had 1.4times of organic matter, 1.5times of N, 1.8times of available P, 2times of exchangeable K, 1.3times of exchangeable Ca, and 1.6times of exchangeable Mg than the top 10cm of soil did. Earthworms have altered the soil texture and increased nutrient availability through production of the soil casts in this limestone area.

• Korean Journal of Soil Science and Fertilizer
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• v.23 no.2
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• pp.140-145
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• 1990

This study was carried out to evaluate the possibllity of composting from cow manure using earthworms. In order to rear earthworms safely and control moisture soil was used as conditioner. Fusarium oxysporum and Fusarium sp.1 were isolated from soil and two isolates of Bacillus sp. were selected as antagonists from earthworm casts. By rearing earthworm, C/N ratio was reduced by 40.9% and 41.9% after 28 days, reducing sugar was also decreased by 37.1% and 37.5%. On the other hand, reduction of C/N ratio in cow manure alone was 9.67% and reducing sugar was 12.01%, respectively. Bacillus sp. was apparently increased in earthworm casts than in ordinary soil. It seemed that the earthworm casts contained antibiotic subatance.

• Korean Journal of Pharmacognosy
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• v.31 no.4
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• pp.373-382
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• 2000

This study was performed to investigate the antithrombotic activity and protective effect of hexane fraction of Kamihyulbuchukeotang (KHCTH) on brain injury by KCN and MCA occlusion a prescription of HCT added with Lumbricus and Notoginseng Radix. Experiemental parameters are brain ischemia by MCA occlusion assay, KCN-induced brain injury, pulmonary thrombosis and platelet aggregation assay. The results were summarized as follows; 1. KHCTH extracts significantly inhibited the duration of KCN-induced coma (67%) and mortality (80%). 2. KHCTH extracts significantly suppressed brain ischemic area and edema following MCA occlusion and protected neuron cells as compared with control data. 3. KHCTH extracts inhibited pulmonary thrombosis induced by collagen and epinephrine. 4. KHCTH extracts inhibited platelet aggregation induced by collagen, ADP as agonist up to 76.9% and 32.3% respectivey at 1 mg/ml more effective than water extract of KHCT These data suggested that KHCTH could be applied as the protector of brain ischemia and injury and antithrombotic agent.

• Proceedings of the KOSOMBE Conference
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• v.1997 no.11
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• pp.482-483
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• 1997

Antifibrinolytic effect of lumbrokinase in mice, in vivo, was studied. Liquid phase lumbrokinse from Lumbricus rubellus was purified by column chromatography method. Lumbrokinase was orally inserted to mice. Although oral dosage of lumbrokinase, to investigate whether lumbrokinase in mice causes antifibrinolytic effect, we have concentration of lumbrokinase varied, and detection of antifibrinolytic effect was carried out using a FDP (fibrin degradation product) test and euglobulin fibrinolytic activity test. FDP test and euglobulin fibrinolytic activity test were compared the data of PBS ingestion. control with lumbrokinase ingestion. As a result, FDP was increased in 7.8mg, 26mg lumbrokinase concentrations after 25 hours succeeding oral prescription of lumbrokinase, and decreased after 49 hours, but 7.8mg lumbrokinase ingestion more increased than 26mg. Also, FDP of PBS ingestion control and 1.3mg lumbrokinase ingestion were not been observed nothing. Euglobulin fibrinolytic activity of PBS ingestion control and 1.3mg lumbrokinase ingestion were not been observed any clear zone after 8, 25, 48 hours, and 7.8mg and 26mg were observed the most largest clear zone after 25 hours and decreased after 48 hours. However, antifibrinolytic effect of oral prescription of lumbrokinase in mice was observed. Now we need to determine an efficient amount of lumbrokinase for antifibrinolytic effect.

• Hwankyungkyoyuk
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• v.13 no.2
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• pp.51-62
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• 2000

This study examined the current status of school environmental education (EE) in Germany, and attempted to propose strategies to develop school EE in Korea. The study intended to accomplish the following goals: First, this study attempted to examine the developmental process of school EE in Germany. Second, exemplary cases of site-based experiential education in Germany are selected and analysed to develop pedagogical strategies in school EE in Korea. The strategies for development proposed in this study can be summarized as follows: First, the focus of EE should go beyond that of "solving or curing"environmental problems and pollutions, and need to be re-directed to pursue an "education for sustainable development", a new paradigm for future EE. Second, EE should increasingly integrate outdoor site-based, experiential programs into the currently classroom-based and knowledge-driven forms of education, not only to maximize its educational effect but also and knowledge-driven forms of education, not only to maximize its educational effect but also to coordinate the instruction of EE in the context of the educational reform agenda, currently undergoing since the late 1990s, in Korea. Third, teaching-learning materials for EE should be variously developed, and programs should be encouraged to serve local needs and circumstances for effective classroom practices, and criteria for standardization and professionalization should be sought in developing these programs. Fourth, it is recommended that the Ministry of Environment secure funds to facilitate the local school authorities at the municipal and provincial levels to operate a mobile environmental support system, equivalent to the function of the "Lumbricus"in Germany. Fifth, an external support network should be established among the support agencies for EE. Especially, a professional agency or institute to academically support school EE and a special planning committee for enhancing EE need to be established for resource and personal development. Parents′voluntary participation should also be encouraged to establish a tripartite network of "school-community-government" to support and facilitate school EE.

• Journal of the Korea Organic Resources Recycling Association
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• v.4 no.2
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• pp.77-85
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• 1996

The study on the ripening of leather waste sludge was performed to vemistabilize the sludge effectively using a laboratory scale darkened wood box reactor ($10{\times}10{\times}20cm$). The acceptable feed conditions for earthworm of Eh, alkalinity were obtained in the 30th and 35th days, respectively. The value of pH was gradually decreased from 7.8 to 7.1 through the ripening time. The contents of heavy metals of the ripened sludge were estimated as lower levels compared to the other regulatory standards for compost. After the ripening time for 50days, the physico-chemical properties of the sludge were estimated as pH 7.1, water content 72%, redox potential 85mV, electrolytic conductivity $2,620{\mu}mhos/cm$ and alkalinity 450 ppm as $CaCO_3$, respectively. In the ripened sludge, survival rate of the earthworms for 50 days was about 75%, and the live weight increase rate was about 230% at the temperature range of $20{\sim}25^{\circ}C$. Moreover, hatching rate of the earthworm cocoons was about 87% and the highest value was obtained in the 20~30th days. From the above results, it was evaluated that leather waste sludge could be vermistabilized effectively by earthworms, when the sludge was ripened during 50 days.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.46 no.2
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• pp.195-204
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• 2020

The purpose of this study was to investigate the effects of 14 Chung-bu medicinal materials described in the Dongui Bogam on inflammatory cytokine production in HaCaT human keratinocyte cells. In order to confirm this possibility, we screened inhibition activity of 17 cytokines using Bio-Plex Pro^TM Human Cytokine 17-plex assay in HaCaT cell lines. Of the 14 Chung-bu medicinal materials, Holotrichia (Ho) and Scorpio (Sc) exerted inhibitory effects on interleukin (IL)-5 production; Ho, Mantidis Ootheca (MO), and Hirudo (Hi) exerted inhibitory effects on IL-6 production; Ho, MO, Lumbricus (Lu), Hi, and Meretricis Concha (MC) showed significant inhibitory effects on IL-8 production; Gecko (Ge), Bombycis Faeces (BF), Cicadidae Periostracum (CP), and MC showed significant inhibitory effects on IL-13 production; and Testudinis Chinemis Plastrum et Carapax (TCPC), BF, and Lu exerted significant inhibitory effects on MIP-1β production. Results indicated that the Chung-bu medicinal materials might be a good candicate as potential anti-inflammatory agents for inhibition of skin inflammation. However, further investigations on these materials, including mechanistic studies, should be carried out to validated the effects in human skin equivalent models of dermatitis.

• The Journal of Dong Guk Oriental Medicine
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• v.8 no.2
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• pp.115-129
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• 2000

The cause that the increase of animality fat intakes, under exercise, fatness, adding the stress, advanced age etc., the occurrence rate of the circulation system disease has been increased. And the thrombosis importantly came to the front as the risk factor of these circulation system's disease. Nowadays, the ischemic disease has especially discussed, for example the angina or myocardial infarction, originated in thrombosis that came from the platelet aggregation. In the western medicine, as the cure and prevention, using the aspirin or ticlopidine for platelet aggregation suppressant. But in the , the curing method must be used properly according to the pectoralgia or heartache's kind, state, grade. The platelet do not attache to the normal hemangioendothelial cell. But when it stimulated by endothelium peronia and so on, it attache to the injury endothelium or rise aggregation between the platelet. On this time, it secrete the platelet aggregation inducer as like ADP, thromboxane A2 from the inside of platelet. So it has first defensive function through the aggregation augment that prevent the celerity consumption of blood. But the activation of abnormal platelet occur the platelet grume and thrombogenesis. So it bring up the occlusive angiosis, so to speak, cardiovascular disease, cerebrovascular disease, arterial sclerosis. In oriental medicine, the thrombosis in the category of blood stasis and this blood stasis present the generalise or local blood circulation disturbance that generated by all kinds of pathological fact or blood stream retention accompanying with a series of syndrome. As the syndrome, stabbing pain fixed at certain region, squamous and dry skin, fullness and pain of the chest and hypochondrium, firmness and fullness of the lower abdomen, black stool, dark purple tongue or with ecchymoses and petechiae etc.. has been created. And it becomes the pathopoiesis cause that the convulsion and palpitation, severe palpitatiion, tympanites, the symtom complex with a mass or swelling in the abdomen, insanity, stricken by wind etc.. Moreover, it used the drugs for invigorating blood circulation and eliminating blood stasis or drugs for removing blood stasis for all kinds of syndrome through the blood stasis. And the drugs for activating the blood circulation, such as Salviae Radix, Angelicae Sinensis Radix, Persicae Semen, Achyranthis Radix, Cnidii Rhizoma, Carthami Flos are used for that. And it is used to the herbs of insects that has strong effect about the disintergrating blood stasis such as Hirudo, Scolopendrae Corpus, Buthus, Lumbricus etc.. On this study, It used Geijibokryunghwan(GBH) and the consisting herbs to investigate the influence of platelet aggregation about drugs that used to improvement various symptoms created by the thrombosis in oriental medicine. GBH formula has as formula recorded in the , action of 'eleminating the evil and not impairment of healthy energy' and 'promoting the flow of QI and cold and heat, so used for the expel blood stasis herbs from the ancient. Therefore we investigated the restraint effect of GBH and the consisting herbs about the platelet agregation induced to the ADP, AA or collagen. The conclusion is following. 1. When it added the aggregation inducer after that it added GBH and individual consisting herbs in the PRP, GBH showed the (+) inhibition effect on the platelet aggregation and it showed the (+) inhibition effect in the individual consisting herbs as like Paeoniae Radix and Moutan Cortex Radicis. 2. It showed the (+), (+,++) inhibition effect on the platelet aggregation in Paeoniae Radix Hoelen, Paeoniae Radix Moutan Cortex Radicis, Hoelen Moutan Cortex Radicis etc. 3. In the aggregation inhibition activating on the difference of density, GBH showed strong inhibition effect to the aggregation state induced to collagen, and it showed the inhibition effect in the individual consisting herbs as like Paeoniae Radix and Moutan Cortex Radicis about the aggregation induced by the collagen. 4. It showed the strong inhibition effect about the aggregation induced by the collagen in Paeoniae Radix Hoelen, Paeoniae Radix Moutan Cortex Radicis, Hoelen Moutan Cortex Radicis etc Like this, as confirm GBH and the individual consisting herb's inhibition effect of platelet aggregation, We considerated that GBH and the individual consisting herbs have practical applicational value of clinical trial in the thrombosis caused by platelet aggregation.