• Journal of Korean Medicine Rehabilitation
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• v.19 no.1
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• pp.91-102
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• 2009

Objectives : The present study was carried out investigate the anti-cancer effect of Salvia miltiorrhiza, Carydalis turtschaminovii and Reynoutria elliptica herbal acupuncture on solid tumor of rats induced by injection of RK3E-ras cells. Methods : RK3E-ras cells were injected on the right lumbar region of rats. After 1 weeks, the experimental rats were divided into four groups : Control group, Salvia miltiorrhiza herbal acupuncture group(SM), Carydalis turtschaminovii herbal acupuncture group(CT), Reynoutria elliptica herbal acupuncture group(RE). And we investigated the weight and size of tumor tissue, gross anatomy, histological and PCNA immunohistochemical study, hepatic and renal metastasis for tumor of each group. Results : 1. In the weight of tumor tissue assessment, SM and CT's weight of tumor tissue was decreased. 2. In the size of tumor tissue assessment, SM was smaller than any other group. 3. In the histological observation, SM's formation of tunica fibrosa that surround the tumor cell was obvious and vasculature that developes circumference of tumor cell was not observed, and density of tumor cell was very low. 4. In the PCNA immunohistochemical study, Control group, SM, RE showed strong immune response in the central site of tumor tissue. 5. In observation of liver and kidney tissue, we were not able to observe tumor cell in the SM. Conclusions : Consequently, SM and CT showed a inhibition of growth and metastasis.

• Physical Therapy Korea
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• v.23 no.1
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• pp.80-86
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• 2016

Background: Prone hip extension (PHE) can be performed to measure the lumbopelvic motor patterns and motions. Imbalances in lumbopelvic muscle activity and muscle weakness can result in instability including pain in lumbopelvic region. The posterior oblique sling (POS) muscles contribute to dynamic lumbopelvic stability. In addition, POS are anatomically aligned with the trapezius muscle group according to shoulder positions. Objects: This study compared the electromyography (EMG) activity of POS and pelvic compensations during PHE with and without pre-activation of lower trapezius muscle (lowT). Methods: Sixteen healthy males were recruited. PHE was performed in randomized order: PHE with and without lowT pre-activation. Surface EMG signals were recorded for biceps femoris (BF), gluteus maximus (GM) (ipsilateral), lumbar multifidus (MF) (bilateral), and the lowT (contralateral). An electromagnetic tracking motion analysis was used to measure the angle of pelvic rotation and anterior tilting. Results: The ipsilateral GM and bilateral MF EMG amplitudes were greater during PHE with lowT pre-activation compared to PHE without lowT pre-activation (p<.05). The BF amplitude during PHE without lowT pre-activation was significantly greater than that during PHE with lowT pre-activation (p<.05). The angles of pelvic rotation and anterior tilting during PHE with lowT pre-activation were significantly smaller compared to PHE without lowT pre-activation (p<.05). Conclusion: PHE with lowT pre-activation, which is aligned with the POS, showed more increased MF and GM muscular activity with smaller lumbopelvic compensations in rotation and anterior tilting compared to PHE without lowT pre-activation.

• The Journal of Korean Physical Therapy
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• v.18 no.6
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• pp.43-49
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• 2006

Purpose: Changes of degenerative disc have been analyzed or with the Magnetic Resonance Imaging (MRI) to assign appropriate meaning, and the relationship between the degenerative changes of the discs and its living function has been evaluated through the living function evaluation scores collected by functional rating index. Methods: in April, 2006, a physical examination was conducted for physical laborers to be employed as on-site laborer in a steel industrial complex located at a region. A month later, these 20 laborers who participated in lumbar vertebra MRI tests have been investigated for one-to-one mobile phone functional rating index test. Excluding 3 of 14 respondents whom scored 0 in functional rating index, the rest of respondents' degenerative disc grade changes shown in MRI and its relationship to living functional rating index have been performed. Results: The Dabbs method of measuring disc height resulted to show significant increase as the disc height changes from L1-2 level to L5-S1 level (p<0.01). However, there was no statistical significance with a relationship between disc height and functional rating index, and disc height average. The Magnetic Resonance Imaging analysis regard on the degree of disc degeneration and its relationship to living functional rating proved to have significant relationship (p<0.05). Conclusion: The degenerative changes monitored by Magnetic Resonance Imaging show significant relationship (p<0.05) to living function. However, this relationship could vary depend upon the characteristics of study population. So, it suggested that the future studies should be performed by considering population's age and job career.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1996.04a
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• pp.175-175
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• 1996

The spinal dorsal horn is the area where primary afferent fibers terminate and cutaneous sensory information is Processed. A number of putative neurotransmitter substances, including excitatory and inhibitory amino acids and peptides, are present in this region and sites and cellular mechanisms of their actions have been a target of numerous studies. In this study, single neurons were acutely isolated and the properties of whole cell current and responses to excitatory and inhibitory neurotransmitters were studied by the patch clamp method. Young rats (7-14 days) were anesthetized with diethyl-ether, and the lumbar spinal cord was excised and cut transversely at a thickness of 30$\mu\textrm{m}$ by Vibroslicer. The treatment of spinal slices with low concentration of proteases (pronase and thermolysin 0.75 mg/$m\ell$) and mechanical dissociation yielded isolated neurons with near intact morphology. Multipolar, ellipsoidal and bipolar, and pyramidal cells were shown. By applying step voltage pulses to neurons held at -70 mV, two types of inward currents and one outward currents observed. The fast activating and inactivating inward current was the Na$\^$+/ current because of its fast kinetics and blocking by 0.5${\mu}$M TTX, a specific blocker of Na$\^$+/ channel. The second type of inward currents were sustained. Based on their kinetics and current-voltage relations, it was likely that the second type of inward current was the voltage-dependent Ca$\^$2+/ current. In the presence of TTX, the steady-state currents mainly represented outward K$\^$+/ current which looked like the delayed rectifier K$\^$+/ current. In addition, the membrane currents produced by agonist of excitatory amino acid (EAA) receptor and the endogenous transmitter candidate L-glutamate were recorded in isolated whole-cell voltage clamped neurons as well as responses to inhibitory amino acids (${\gamma}$-amino butyric acid, glycine). Drugs were applied by a method that allows complete exchange of the solution within 1 sec; an infinite number of solutions can be applied to a single cell.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.7
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• pp.1108-1114
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• 2007

Twenty eight non-lactating and non-pregnant adult Serra da Estrela ewes, ranging in body condition score (BCS) from 1 to 4 were used to study the relationships between BCS, live weight (LW), body composition and fat partition. Ewes were slaughtered and their kidney knob and channel fat (KKCF), sternal fat (STF) and omental plus mesenteric fat (OMF) were separated and weighed. Left sides of carcasses as well as the respective lumbar joints were then dissected into muscle, bone and subcutaneous (SCF) and intermuscular fat (IMF). The relationship between LW and BCS was studied using data from 1,396 observations on 63 ewes from the same flock and it was found to be linear. Regression analysis was also used to describe the relationships among BCS and/or LW and weights (kg) and percentages in empty body weight (EBW) of dissected tissues. The prediction of weights and percentages in EBW of total fat (TF) and of all fat depots afforded by BCS was better than that provided by LW. Only the weight of muscle and the percentage of bone in the EBW were more efficiently predicted by LW than by BCS. IMF represented the largest fat depot with a BCS of 1 and 2, whereas SCF was the most important site of fat deposition with a BCS of 3 and 4. Allometric coefficients for each fat depot in TF suggest that the fat deposition order in ewes from this breed is: IMF, OMF, SCF and KKCF. Results demonstrate that BCS is a better predictor than LW of body reserves in this breed and that LJ is a suitable anatomical region to evaluate BCS.

• Physical Therapy Korea
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• v.3 no.2
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• pp.19-28
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• 1996

The purpose of this study was to asses and compare the effects of superficial heating on the pain threshold at limbs in healthy adult and adult hemiplegia. We used hot pack for superficial heat and applied to healthy adult(n=12) and adult hemiplegia(n=12) on lumbar region. Pain treshold was quantatively measured by an electrical stimulator and measured before hot pack application, immediatly and post 30 minutes after hot pack application on the distal parts of limbs(styloid process of radius, medial malleoulus of tibia). The results were as follows 1) A statically significant defference in pain treshold were not found at limbs of healthy adult and adult hemiplegia that have an affected side and a non-affected side before hot pack application, immediatly and post 30 minutes after hot pack apllication(p>0.05). 2) In comparance of pain threshold of upper and lower limbs in the all subjects, pain threshold was significantly increase at lower limbs(p<0.01). 3) In comparance of pain threshold of limbs between healthy adult and adult hemiplegia, pain threshold was showed a significant defferance at the upper and lower limbs of affected side before hot pack application(p<0.05, p<0.01).

• The Journal of Churna Manual Medicine for Spine and Nerves
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• v.2 no.1
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• pp.69-75
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• 2007

Objectives : This study was performed to find the correlation between moire topography and muscle development ratio of both lower limbs. Methods : 88-general persons who examed general health checkup were enrolled this study. Who coincided the exclusion criteria were left out. The authors practiced Moire Topography by using IBS-2000 and measured difference of contour line and interval between vertical base line of neck and pelvis. We made use of X-SCAN to analyse body component and calculate muscle development ratio of both lower limbs. Results : In this study, we found out that the more interval between vertical base line of neck and pelvis grown large, the more muscle development ratio of both lower limbs got small. So, there was correlation between imbalance of the dorsum muscle and muscle development ratio of both lower limbs.(p>0.05). But others did not have. Conclusion : According to this results, we found out there was correlation between imbalance of lumbar and gluteal region and muscle development ratio of both lower limbs. Apply to clinic, we are able to improve the efficacy of diagnosis and medical treatment.

• The Korean Journal of Pharmacology
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• v.32 no.2
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• pp.283-292
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• 1996

The spinal dorsal horn is the area where primary afferent fibers terminate and cutaneous sensory information is processed. A number of putative neurotransmitter substances, including excitatory and inhibitory amino acids and peptides, are present in this region. In this study, single neurons of the spinal dorsal horn were acutely isolated and the properties of whole cell current and responses to excitatory and inhibitory neurotransmitters were studied by patch clamp technique. Transverse slice ($(300{\mu}m$) of lumbar spinal cords from young rats$(7{\sim}14\;days)$ were sequentially treated with two pretenses(pronase 0.75 mg/ml and thermolysin 0.75 mg/ml), then single neurons were mechanically dissociated. These neurons showed near-intact morphology such as multipolar, ellipsoidal and bipolar, and pyramidal cells and we recorded the typical whole cell currents of $K^+$, $Ca^{2+}$ and ligand-operated channels from these neurons. Glutamate $(30{\mu}M)$ and N-methyl-D-aspartate(NMDA, $30{\mu}M)$ induced inward currents of $117{\pm}12.4$ pA(n=5) and $49{\pm}6.9$ pA(n=3), respectively. Glycine $(1{\mu}M)$ potentiated glutamate-induced currents $4{\sim}5$ times and NMDA-induced currents $8{\sim}10$ times. In addition, glycine $(30{\mu}M)$ induced Inward current ($31{\pm}6.1$ nA, n=2), which was rapidly desensitized after the peak to a new steady-state level. However, the inward currents induced by ${\gamma}-amino$ butyric acid(GABA, $1{\mu}M$) decreased continuously after the peak($226{\pm}41.6$ pA, n=3) under the similar experimental condition. The ionic currents and pharmacological responses of isolated neurons in this work were similar to those observed in vivo or in vitro spinal cord slice, indicating that acutely isolated neurons could be effectively used for further pharmacological studies.

• The Korean Journal of Pain
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• v.2 no.1
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• pp.21-25
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• 1989

For the simple operations of the perineal region, caudal, epidural and saddle blocks of analgesia are preferable anesthesia techniques in men. But, if the operations shoud be delayed or the physicians are willing to observe and manipulate the patients for a couple of days, as in the ureteral stone manipulation, continuous epidural analgesia will be a reliable method. The authors have observed 36 male patients of ureteral calculi with continuous lumbar epidural analgesia for the purpose of elimination of an undesirable pain sensation which was associated with the transurethral cystoscopic manipulation. The results were as follows: 1) In most cases, the anesthetics used were 1% lidocaine 20 ml through the $L_{3-4}$ and $L_{4-5}$ interspace with 4~6cm of catheter tip advancement mainly to cephalad. 2) There was no motor weakness or paralysis in the lower extremities except some numbness and paresthesia on the perineal area. 3) Besides the various minor complications, there were no problems in respiration with small doses of morphine extradurally. 4) Among them, four cases of ureteral calculi dropped into the bladder spontaneously due to the epidural technique during transportation of the patients from the pain clinic to the urology out-clinic. One case of calculi was dropped by the aid of instrumentation at the ward, and twelve cases of calculi were removed directly by Dormia stone basket in the scopic room. For the remaining patients, we deduced that their calculi was be evacuated out because there were no complaints were notified after discharge.

• Imaging Science in Dentistry
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• v.42 no.4
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• pp.231-235
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• 2012

Purpose: This study was performed to evaluate the trabecular pattern on panoramic radiographs to predict age-related osteoporosis in postmenopausal women. Materials and Methods: Thirty-one postmenopausal osteoporotic women and 25 postmenopausal healthy women between the ages of 50 and 88 were enrolled in this study. The bone mineral density (BMD) of the lumbar vertebrae and femur were calculated using dual-energy X-ray absorptiometry (DXA), and panoramic radiographs were obtained. Fractal dimension (FD) was measured using the box counting method from 560 regions of interest ($51{\times}51$ pixels) in 6 sites on the panoramic radiographs. The relationships between age and BMD and between FD and BMD were assessed, and the intraobserver agreement was determined. Results: There was a significant difference in the FD values between the osteoporotic and normal groups (p<0.05). There was a significant difference in the FD values at three sites in the jaws (p<0.05). Age was significantly correlated with the BMD measurements, with an odds ratio of 1.25. However, the FD values were not significantly correlated with the BMD measurements, with an odds ratio of 0.000. The intraobserver agreement showed relatively higher correlation coefficients at the upper premolar, lower premolar, and lower anterior regions than the other sites. Conclusion: Age was an important risk factor for predicting the presence of osteoporosis in postmenopausal women. The lower premolar region was the most appropriate site for evaluating the FD value on panoramic radiographs. However, further investigation might be needed to predict osteoporosis using an FD value on panoramic radiographs.