• BMB Reports
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• 제49권7호
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• pp.357-358
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• 2016

Merlin, encoded by the NF2 gene, is a tumor suppressor that exerts its function via inhibiting mitogenic receptors at the plasma membrane. Although multiple mutations in Merlin have been identified in Neurofibromatosis type II (NF2) disease, its molecular mechanism is not fully understood. Here, we show that Merlin interacts with LRP6 and inhibits LRP6 phosphorylation, a critical step for the initiation of Wnt signaling. We found that treatment of Wnt3a caused phosphorylation of Merlin by PAK1, leading to detachment of Merlin from LRP6 and allowing the initiation of Wnt/β-catenin signaling. A higher level of β-catenin was found in tissues from NF2 patients. Enhanced proliferation and migration caused by knockdown of Merlin in glioblastoma cells were inhibited by suppression of β-catenin. Conclusively, these results suggest that sustained Wnt/β-catenin signaling activity induced by abrogation of Merlin-mediated inhibition of LRP6 phosphorylation might be a cause of NF2 disease.

• Biomolecules & Therapeutics
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• 제29권5호
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• pp.527-535
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• 2021

Sclerostin (SOST), a regulator of bone formation in osteocytes, inhibits the canonical Wnt signaling by interacting with low-density lipoprotein receptor-related protein 5/6 (LRP5/6) to prevent Wnt binding. Loss-of-function mutations of the SOST gene caused massive bone outgrowth and SOST-null mouse exhibited a high bone density phenotype. Therefore, SOST has been suggested as a promising therapeutic target for osteoporosis. A few previous studies with X-ray crystallography identified the binding interfaces between LRP6 and SOST, but there are limitations in these studies as they used truncated SOST protein or SOST peptide. Here, we analyzed the conformational dynamics of SOST-LRP6 E1E2 complex using hydrogen/deuterium exchange mass spectrometry (HDX-MS). We examined the effect of the C-terminal tail of SOST on LRP6 conformation upon complex formation. HDX-MS analysis suggested a new potential binding interface for the C-terminal region of SOST that was missing from the previous crystal structure of the SOST-LRP6 E1E2 complex.

• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.6311-6314
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• 2013

Background: While 5-port laparoendoscopic radical prostatectomy is standard practice, efforts have been focused in developing a single port surgery for cosmetic reasons. However, this is still in the pioneering stage considering the challenging nature of the surgical procedures. We have therefore focused on reduced port surgery, using only 2-ports. In this study, we compared 2-port laparoendoscopic radical prostatectomy (2-port RP) and conventional 5-port laparoscopic radical prostatectomy (LRP) for clinically localized prostate carcinoma and evaluated the potential advantages of each. Materials and Methods: From January 2010 to December 2010, all 23 patients with clinically localized prostate cancer underwent LRP. Starting November, 2010, when we introduced the reduced port approach, we performed this procedure for 22 consecutive patients diagnosed with early-stage prostate cancer (cT1c, cT2N0). The patients were matched 1:1 to 2-port RP or LRP for age, preoperative serum PSA level, clinical stage, biopsy and pathological Gleason grade, surgical margin status, pad-free rates and post-operative pain. Results: There was a significant difference in operative time between the 2-port RP and LRP groups ($286.5{\pm}63.3$ and $351.8{\pm}72.4$ min: p=0.0019, without any variation in blood loss (including urine) ($945.1{\pm}479.6$ vs $1271.1{\pm}871.8ml$: p=0.13). The Foley catheter indwelling period was shorter in the 2 port RP group, but without significance ($5.6{\pm}1.8$ vs $8.0{\pm}5.6$ days: p=0.057) and the total perioperative complication rates for 2 port RP and LRP were comparable at 4.5% and 8.7% (p=0.58). There was an improvement in pad-free rates up to 6 months follow-up (p=0.090), and significantly improvement at 1 year (p=0.040). PSA recurrence was 1 (4.5%) in 2-port RP and 2 (8.7%) in LRP. Continuous epidural anesthesia was used in most of LRP patients (95.7%) and in early 2-port RP patients (40.9%). In these patients, average total amount of Diclofenac sodium was 27.8mg/patient in 2-port RP and 50.0mg/patient in LRP. Conclusions: Thus the reduced port approach is as efficacious as LRP in terms of many outcome measures, with significant cosmetic advantages and reduction in post surgical pain. This method can be readily performed safely and therefore can be recommended as a standard laparoscopic surgery for prostate cancer in the future.

• 동아시아식생활학회지
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• 제20권4호
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• pp.516-523
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• 2010

This study was conducted to investigate the physicochemical and sensory characteristics of tofu prepared with the addition of lotus root powder (LRP). The moisture, crude ash, carbohydrate, crude protein, and crude lipid contents of the lotus root powder were 8.29%, 4.73%, 30.66%, 47.84%, and 8.48%, respectively. The yield rate of the tofu did not differ significantly according to the level of added LRP; however, there was a significant decrease in pH and a significant increase in acidity. The L and b values of the tofu decreased as the amount of LRP in the formulation increased, whereas the a value increased. Furthermore, hardness and cohesiveness increased significantly as the level of LRP increased. In sensory evaluation, LRP tofu had low scores in air cell size and a beany smell, but high scores in hardness. In terms of overall acceptability, the preferred tofu samples were the control and that containing 0.3% lotus root powder.

• Journal of Microbiology and Biotechnology
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• 제13권1호
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• pp.159-163
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• 2003

An lrp gene encoding a leucine-responsive regulatory protein was identified from Vitro vulnificus, and its role in the survival of the organism was assessed by analyzing the stress tolerance of the isogenic mutant, in which the lrp gene had been inactivated. The results demonstrated that Lrp contributes to the survival of V. vulnificus is dependent of the phase of growth.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2009년도 춘계학술대회 논문집
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• pp.941-942
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• 2009

• The Korean Journal of Physiology and Pharmacology
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• 제19권3호
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• pp.235-240
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• 2015

Androgen receptor (AR) signaling is important for prostate cancer (PCa) cell proliferation. Here, we showed that proliferation of hormone-sensitive prostate cancer cells such as LNCaP was significantly enhanced by testosterone stimulation whereas hormone-insensitive prostate cancer cells such as PC3 and VCaP did not respond to testosterone stimulation. Blocking of AR using bicalutamide abolished testosterone-induced proliferation of LNCaP cells. In addition, knockdown of AR blocked testosterone-induced proliferation of LNCaP cells. Basal expression of low-density lipoprotein receptor-related protein 6 (LRP6) was elevated in VCaP cells whereas stimulation of testosterone did not affect the expression of LRP6. However, expression of LRP6 in LNCaP cells was increased by testosterone stimulation. In addition, knockdown of LRP6 abrogated testosterone-induced proliferation of LNCaP cells. Given these results, we suggest that androgen-dependent expression of LRP6 plays a crucial role in hormone-sensitive prostate cancer cell proliferation.

• 동아시아식생활학회지
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• 제22권1호
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• pp.33-40
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• 2012

본 연구는 연근 및 연잎분말의 첨가가 돈육 patty의 품질 특성에 미치는 영향을 검토하였다. 돈육 patty는 돈육 88%, 대두유 5%, 냉수 5%, 소금 2%를 배합하여 제조한 대조군(CTL), 여기에 연근분말 0.5% 첨가한 것(LRP), 연근분말 0.25% 및 연잎분말 0.25% 첨가한 것(LRLP), 그리고 연근분말 0.5% 첨가한 것(LLP) 등 네 종류의 돈육 patty를 제조하였다. 그리고 일반성분, 총 폴리페놀화합물 함량, 색깔, 기계적 조직감, 보수력, 가열감량, 두께 및 직경 변화, pH, VBN 함량, TBARS 및 총균수를 측정하였다. 수분함량은 CTL, 단백질 함량은 LRLP, 지방함량은 LRLP 및 LLP, 그리고 회분함량은 LRLP가 가장 높았다(p<0.05). CTL, LRP, LRLP 및 LLP의 총 폴리페놀 함량은 각각 3.34, 3.64, 3.90 및 3.90 mg/100 g이었다. 명도($L^*$)는 시료들 사이에 유의한 차이가 없었으며, 적색도($a^*$)는 CTL이 가장 높았고, 황색도는 LRLP 및 LLP가 CTL 및 LRP보다 높았다(p<0.05). 경도는 CTL 및 LRP가 LRLP 및 LLP보다 높았으며, 탄성은 LLP가 가장 낮았고, 응집성, 검성 및 저작성은 시료들 사이에 유의한 차이가 없었다. 보수력은 시료들 사이에 유의한 차이가 없었으나, 가열감량, 두께 및 직경 변화는 CTL이 가장 높았다. pH는 CTL이 가장 높았다(p<0.05). VBN 함량과 총균수는 시료들 사이에 유의한 차이가 없었다. 그리고 TBARS는 CTL이 가장 높고, LLP가 가장 낮았다(p<0.05).

• 디지털융복합연구
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• 제17권10호
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• pp.321-327
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• 2019

본 연구의 목적은 언어장애 환자군의 의사소통 능력 향상을 위하여 스마트 기기 기반의 언어재활 프로그램 개발에 있다. 이를 위하여 기존에 개발된 다양한 국내외 의사소통 언어훈련 프로그램을 비교분석하여 구체적인 의사소통 범위 및 훈련 과제, 문항 등을 검토하여 과제 구성에 필요한 콘텐츠를 선별하였다. 다양한 의미범주와 문법요소를 포함하고 언어학적 단위에 기초하여 단어수준, 의미범주, 문장수준, 담화수준의 이해산출 능력 향상을 위하여 17개의 의미범주를 추출하고 29개의 훈련과제와 3780개의 훈련 문항을 제작하였으며, 윈도우를 기반으로 하는 관리 프로그램과 안드로이드 기반 재활 훈련 애플리케이션을 개발하였다. 본 언어재활 프로그램은 가정에서 쉽고 편리하게 홈 프로그램으로 사용되어 언어훈련 효과를 증대시킬 수 있으며, 환자 관리 및 훈련 결과 데이터베이스 구축 등 언어장애 환자군의 치료에 유용하게 사용될 것으로 기대된다. 향후 언어장애 환자군 별 언어재활 효과 검증 등의 후속 연구가 진행될 예정이다.

• 한국멀티미디어학회논문지
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• 제24권8호
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• pp.1122-1137
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• 2021

PM_2.5 concentration in Seoul could be predicted by deep neural network model. In this paper, the contribution of input factors to the model's prediction results is analyzed using the LRP(Layer-wise Relevance Propagation) technique. LRP analysis is performed by dividing the input data by time and PM concentration, respectively. As a result of the analysis by time, the contribution of the measurement factors is high in the forecast for the day, and those of the forecast factors are high in the forecast for the tomorrow and the day after tomorrow. In the case of the PM concentration analysis, the contribution of the weather factors is high in the low-concentration pattern, and that of the air quality factors is high in the high-concentration pattern. In addition, the date and the temperature factors contribute significantly regardless of time and concentration.