• 대한한방소아과학회지
• /
• 제19권2호
• /
• pp.175-195
• /
• 2005

Objective: This experimental study was performed to examine the in vitro and in viva anti-inflammatory and anti-allergic effects of Mori Cortex. Methods: Water extract of Mori Cortex was studied to its ability to stimulate or inhibit macrophage 264.7 cells to produce inflammatory and allergic mediators. Cytokines such as $IL-1{\beta}$, IL-6, IL-10 and $TNF-{\alpha}$ were measured by immunochemical assay. In vitro, the macrophages 264.7 were classified into four groups. One group was a normal group. The other group was a (-) control group stimulated with LPS. And the third group was a (+) control group pretreated for 1 hour with hydrocortisone. And the fourth group was a sample group pretreated for 1 hour with Mori Cortex. After pretreatment, macrophage were incubated with lipopolysaccharide(LPS) $100\;ng/m{\ell}$ for 12 hour and media collected and $IL-1{\beta}$, IL-6, IL-10 and $TNF-{\alpha}$ concentrations in supernatants were measured each by Enzyme linked immuno-soubent assay. Mori Cortex were used $50\;{\mu}g/m{\ell},\;100\;{\mu}g/m{\ell},\;250\;{\mu}g/m{\ell},\;500\;{\mu}g/m{\ell},\;and\;1,000\;{\mu}g/m{\ell}$. Hydrocortisones were used $10^{-8}M,\;10^{-7}M,\;10^{-6}M,\;10^{-5}M\;and\;10^{-4}M$. In vivo, the SD rats were classified into three groups. One group was a normal group injected with normal saline into the abdominal cavity. The other was a control group prescribed to compound 48/80 after normal saline injection. And the third was a sample group prescribed to compound 40/80 after Mori Cortex injection. Then, the release of histamine, IL-6 and $TNF-{\alpha}$ were measured. Results : In vitro, Man Cortex significantly increased the release of $IL-1{\beta}\;and\;TNF-{\alpha}$ by LPS-stimulated macrophage 264.7 cells. And it significantly decreased the release of IL-10. In IL-6, Mori Cortex of low concentration significantly decreased the release of IL-6, but that of high concentration acted in reverse. In vivo, Man Cortex didn't show significant inhibitory effects on the release of histamine and IL-6 in comparison with that of the control group. But it significantly increased the release of $TNF-{\alpha}$ in comparison with that of the control group.

• 한방재활의학과학회지
• /
• 제29권2호
• /
• pp.115-133
• /
• 2019

Objectives The purpose of this study was to evaluate the effects of Curcumae Longae Rhizoma pharmacopuncture on the monosodium iodoacetate (MIA)-induced osteoarthritis rats. Methods Osteoarthritis was induced by injection of MIA ($50{\mu}L$ with 80 mg/mL) into knee joint cavity of rats. Rats were divided into 6 groups. Normal group was injected by normal saline into knee joint cavity only. Control group was induced for osteoarthritis by MIA and orally administered with distilled water. Normal Saline group was induced for osteoarthritis by MIA and injected with normal saline $100{\mu}L$. Positive comparison group was injected with MIA and orally administered with indomethacin 5 mg/kg. Curcumae Longae Rhizoma pharmacopuncture low concentration (CL) group was induced for osteoarthritis by MIA and injected with Curcumae Longae Rhizoma pharmacopuncture low concentration $100{\mu}L$. Curcumae Longae Rhizoma pharmacopuncture high concentration (CH) group was induced for osteoarthritis by MIA and injected with Curcumae Longae Rhizoma pharmacopuncture high concentration $100{\mu}L$. Curcumae Longae Rhizoma pharmacopuncture was injected at ST35 and EX-LE4 each group (CL, CH). After that, hind paw weight distribution was measured and oxidative stress biomarker in serum, liver function biomarker in serum, western blot analysis were measured. Histological analysis of knee joint tissue was performed by hematoxylin and eosin staining, Safranin-O staining and Masson's trichrome staining. Results Hind paw weight distribution was significantly improved in both group. alanine aminotransferanse and aspartate aminotransferase were decreased significantly in CH group compare with Indomethacin threated group. Antioxidant enzyme glutathione peroxidase, Catalase and heme oxygenase-1 were increased in CH group compare with control group. Inflammatory cytokine cyclooxygenase-2, inducible nitric oxide synthase and interleukin-1 beta were decreased significantly in CH group. Histological analysis result shows that protective effects of joint and cartilage were observed in both CH and CL groups in a concentration-dependent. Conclusions The result suggest that Curcumae Longae Rhizoma pharmacopuncture has anti-oxidation effect, anti-inflammatory effect and also can prevent progression of osteoarthritis and protect joint cartilage.

• 한국광학회지
• /
• 제29권6호
• /
• pp.241-246
• /
• 2018

본 논문에서는 광섬유 브래그 격자(FBG)를 이용한 광온도센서를 제작하였으며 광원은 출력 파장의 온도 의존성을 가지는 저가형 VCSEL이 사용되며 FBG에서 반사되는 빛의 파장을 분석하는 interrogator는 VCSEL에서 변화되는 출력 파장을 VCSEL의 내부 온도로 확인하는 방법을 적용하여 저가격의 광온도센서를 구현하였다. VCSEL의 내부온도를 $52.2^{\circ}C$에서 $14^{\circ}C$까지 조절하면서 출력 파장을 1519.90 nm에서 1524.25 nm까지 총 4.35 nm 파장을 변화시켰으며 온도 조절에 따른 파장 변화 반복도 오차는 ${\pm}0.003nm$이며 온도 측정 오차는 ${\pm}0.18^{\circ}C$로 측정되었다. 광온도센서를 사용하여 $22.3{\sim}194.2^{\circ}C$의 온도를 측정한 결과 인가한 온도 ${\Delta}T$에 따른 광원 내부 온도 변화 값은 $0.146^{\circ}C/{\Delta}T$이고 인가한 온도 ${\Delta}T$에 따른 온도 프로브 반사 파장 변화 값(${\Delta}{\lambda}_{\beta}T/{\Delta}T$)은 $16.64pm/^{\circ}C$로 측정되었으며 센서의 측정 오차는 ${\pm}1^{\circ}C$로 나타났다. VCSEL의 출력 파장은 온도에 의존성을 가지고 있어 좁은 범위의 출력 파장을 변화시키기 위한 광원으로 사용하기에 매우 적합하다.

• 한국잠사곤충학회지
• /
• 제24권2호
• /
• pp.55-72
• /
• 1983

가잠의 휴면성을 난각의 조직학적 특성을 보고자 난각층에 대한 전자현미경상을 관찰하였고 또한 난각의 물리화학적 특성에 대해서는 수분투과성, 염산투과성, 염색성 및 난각 구조단백질을 조사한 결과를 요약하면 다음과 같다. 1. 난각구조를 전자현미경으로 보면 뚜렷이 4층으로 되어 있으며, 또한 휴면난과 비휴면난 및 즉침난 사이에 구조적인 차이가 보인다. 즉 외층 밖에 또 다른 전자밀도가 낮은 얇은 층이 휴면난에는 존재하고 있으나 비휴면 및 즉침난에는 이 층을 볼 수 없다. 2. 산난후 20∼24시간째의 휴면난과 비휴면난 및 즉침난 사이에 난각염산투과성의 차이를 보면 휴면난에 비해 즉침난 및 비휴면난이 모두 투과성이 높았고 또한 난령이 지남에 따라 휴면난은 투과성이 떨어지는데 반하여 즉침난 및 비휴면난은 투과성이 높아졌다. 3. 휴면난의 ether 처리난은 무처리난에 비해 난각의 투과성이 변하는 것으로 보아 난각물질 중 ether 용출물이 존재한다. 4. 난각의 수분투과성도 염산투과성에 있어서와 같이 휴면난이 즉침난 및 비휴면난에 비해 수분투과성이 낮았다. 5. 가잠난각의 조직화학적 특성은 단백질과 당에 대한 염색성이 높아 난각에는 단백질과 당이 풍부하며 특히 지질에 대한 염색성은 거의 음성이나 내층으로 갈수록 진하게 염색되었다. 6. 누에의 난각점착물은 PAS-alcian 반응으로 보아 mucopolysaccharides이다. 7. 난각 구조단백질 중 SH단백질에 대한 전기영동상에 있어서 휴면난과 비휴면난 모두 2개의 band가 인정되나 비휴면난의 경우 이동거리가 빠른 band는 매우 흔적적이며 또한 영동거리도 원점으로부터 멀리 이동하여 두 처리간에 minor component에 차이가 보였다. 8. SH단백질에 대한 아미노산조성에 있어서는 휴면난 및 비휴면난 사이에 큰 차이를 인정할 수 없었고 난각단백질중 SH단백질의 구성아미노산에는 glycine이 가장 많고 cystine함량은 적었다. SI K-2 는 Penicillin, Ampicillin과 Cephalothin에 저항성을 나타냈고 ts-U171, A-N92와 A-N115는 이 세항생제에 민감성을 나타냈다. Temperature - sensitive돌연변이균주를 42$^{\circ}C$에서 24시간 배양후에 28$^{\circ}C$로 옮기어 배양한 결과 ts-U601, -U603, -U604와 -U171은 성장을 더 이상하지 않았다.ta-exotoxin을 생산했는데, 48시간 배양 배지 $m\ell$당 70$\mu\textrm{g}$을 분비한 균주는 BTK-1이고, BTK-37 균주는 $m\ell$당 88$\mu\textrm{g}$(6.1$\times$$10^{8}$ Cells/$m\ell$) BTK-35 균주는 $m\ell$당 81$\mu\textrm{g}$(5.2$\times$$10^{8}$ Cells/$m\ell$)을 생산했고. 그외는 모두 70$\mu\textrm{g}$미만이었다. 3. Beta-exotoxin과 B. thuringiensis 균체을 동시에 per os, interaperitoneal injection, subcuntaneous injection, nasal cavity inoculation, intracerebral injection을 120시간 처리했어도 치사효과를 나타내지 않았다.삼각호마법 and Japan#s 병음한자변환방식 have prospect to be prevalent. The following suggestions can be made from these results, 1) All the