• 한국약용작물학회지
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• 제18권3호
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• pp.135-142
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• 2010

Low quality fresh ginseng was fermented by Pheliuus linteus mycelium at $22^{\circ}C$ for 30 days, then extracted by water solvent at $100^{\circ}C$ for 180 min. On human normal cell lines (HEK293), cytotoxicity was about 10% lower in adding extracts of the fermentation ginseng than that from low quality ginseng. The fermented extracts also inhibited the growth of several human cancer cells. Among them, respectively, digestive organs related cancer cells, such as human stomach adenocarcnioma and human epithelial adenocarcinoma were most effectively inhibited up to 85% and 90%, respectively. Then, selectivities were in the ranges of 3 to 5, compared to 2 to 3 from low quality fresh ginseng. Generally, fermented ginseng extract showed higher anticancer activities as well as higher DPPH radical sacavening activity, possibly due to high contents of total phenolic components as 6.96 mg/g. It was very interesting that the fermented ginseng contained very higher contents of ginsenoside-Rc+$Rb_2$, compared to others in low quality fresh ginseng because of partition digestion of mycelium growth. The results can tell that low quality fresh ginseng can be utilized by the fermentation with Pheliuus linteus mycelium.

• 한국약용작물학회지
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• 제22권3호
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• pp.223-230
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• 2014

This study was performed to enhance contents of low molecular ginsenoside using steaming and fermentation process in low quality fresh ginseng. For increase in contents of Rg2, Rg3, Rh2 and CK in low quality fresh ginseng, a steaming process was applied at $90^{\circ}C$ for 12 hr which was followed by fermentation process at Lactobacillus rhamnosus HK-9 incubated at $36^{\circ}C$ for 72 h. The contents of ginsenoside Rg1, Rb1, Rc, Re and Rd were decreased with the steaming associated with fermentation process but ginsenoside Rg2, Rg3, Rh2 and CK increased after process. It was found that under the steaming associated with fermentation process, low molecule ginsenosides such as Rg2, Rg3, Rh2 and CK were increased as 3.231 mg/g, 2.585 mg/g and 1.955 m/g and 2.478 mg/g, respectively. In addition, concentration of benzo[${\alpha}$]pyrene in extracts of the low quality fresh ginseng treated by the complex process was 0.11 ppm but it was 0.22 ppm when it was treated with the steaming process. This result could be caused by that the most efficiently breakdown of 1,2-glucoside and 1,4-glucoside linkage to backbone of ginsenosides by steaming associated with fermentation process. This results indicate that steaming process and fermenration process can increase in contents of Rg2, Rg3, Rh2 and CK in low quality fresh ginseng.

• Journal of Ginseng Research
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• 제8권2호
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• pp.167-171
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• 1984

Soft X-ray is useful to identify the quality of fresh ginseng causing the inside cavity or white pan of red ginseng. The portion of low mass density identified by the difference in absorption of soft X-ray showed lower dry matter density and less or no response to iodine test indicating less accumulation or excess consumption of starch. The inside white part of red ginseng absorbed less X-ray than the normal part did. Probability for identification of the inside cavity or white at fresh ginseng was rather high (80-90%) in screen observation than f'3m reading and seemed to be increased further by using the developed screen and with training. The inside white of red ginseng appeared to be due to starch deficiency. Dry matter density appeared to be better than fresh weight density for the quality criterion.

• 한국약용작물학회지
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• 제23권6호
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• pp.431-438
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• 2015

Background : This study was conducted to determine out the effect of storage temperature on the quality of fresh ginseng (Panax ginseng C. A. Meyer) during distribution. Methods and Results : Fresh ginseng was washed, packed in $30{\mu}m$ low density polyethylene (LDPE) film, then stored at 0, -2 and $-4^{\circ}C$. After 4 weeks of storage, ginseng was then stored at $5^{\circ}C$, as a simulation of the distribution process. Ginseng stored at $-4^{\circ}C$ showed higher respiration rate, ethylene production and electrolyte conductivity during the distribution phase than those stored at 0 and $-2^{\circ}C$. Decay and browning rate rapidly increased following 3 weeks of distribution in samples stored $-4^{\circ}C$. However ginseng stored $-2^{\circ}C$, which is below freezing point, for 4 weeks did not show the physiological change or quality deterioration. Ginsenoside contents decreased during storage for all plant, but did not differ significantly between storage temperatures. Conclusions : Storage at temperatures below $-2^{\circ}C$ can negatively affect respiratory characteristics and electrolyte leakage and increase quality deterioration and decay rates during distribution.

• 한국식품저장유통학회지
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• 제1권2호
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• pp.93-98
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• 1994

Fresh ginseng was packed by using available commercial plastic films of polyvinyl chloride (PVC), cast polypropylene (CPP) and low density polyethylene (LDPE). PVC was used as a wrapping on polystyrene tray, and CPP and LDPE were applied as pouch packages. Gas composition of the package and keeping quality of ginseng in the packages were evaluated for 20 day storage at 5$^{\circ}C$. LDPE (27$\mu\textrm{m}$ thick) Package modified the Package atmosphere to 11.7% O2 and 2.1% CO2, gave low microbial growth and good sensory score after 20 day storage.

• Journal of Ginseng Research
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• 제25권3호
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• pp.122-126
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• 2001

수삼을 물로 세척한 후 한 뿌리씩 연포장재 필름 주머니(ONY/LDPE/L-LDPE, 200$\times$300mm, 90$\mu\textrm{m}$)에 넣고 밀봉하여 $25^{\circ}C$, 1$0^{\circ}C$ 또는 4$^{\circ}C$에서 저장하면서 외관품질과 개체무게를 조사한 결과 수삼의 외관품질은 저장온도에 관계없이 주로 연화에 의하여 불량해졌으며 외관품질 변화 속도상수와 저장온도의 역수뿐만 아니라 개체무게 변화속도상수와 저장온도의 역수간에도 각각 5% 유의수준에서 양호한 직선관계를 나타내었다. 따라서 $25^{\circ}C$에서의 온도학대시험을 통하여 저온에서 저장중인 개별포장 수삼의 저장수명 및 개체무게 변화율을 단기간에 예측할 수 있을 것으로 판단된다.

• 한국약용작물학회지
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• 제17권6호
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• pp.397-406
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• 2009

The low quality fresh ginseng was extracted by water at $80^{\circ}C$ and 240 bar for 20 min (HPE, High pressure extraction process). The cytotoxicity on human normal kidney cell (HEK293) and human normal lung cell (HEL299) of the extracts from HPE showed 28.43% and 21.78% lower than that from conventional water extraction at $100^{\circ}C$ in adding the maximum concentration of $1.0\;mg/m{\ell}$. The human breast carcinoma cell and lung adenocarcinoma cell growth were inhibited up to about 86%, in adding $1.0\;mg/m{\ell}$ of extracts from HPE. This values were 9-12% higher than those from conventional water extraction. On in vivo experiment using ICR mice, the variation of body weight of mice group treated fresh ginseng extracts from HPE of 100 mg/kg/day concentration was very lower than control and other group. The extracts from HPE was showed longer survival times as 35.65% than that of the control group, and showed the highest tumor inhibition activities compared with other group, which were 70.64% on Sarcoma-180 solid tumor cells. On the high performance liquid chromatogram (HPLC), amount of ginsenoside-$Rg_2$, $Rg_3$, $Rh_1$ and $Rh_2$ on fresh ginseng were increased up to 43-183% by HPE, compared with conventional water extracts. These data indicate that HPE definitely plays an important role in effectively extracting ginsenoside, which could result in improving anticancer activities. It can be concluded that low quality fresh ginseng associated with this process has more biologically compound and better anticancer activities than that from normal extraction process.

• 한국약용작물학회지
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• 제20권2호
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• pp.117-123
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• 2012

This study compared the contents of low molecular ginsenoside according to fermentation process in low grade fresh ginseng. Low grade fresh ginseng was directly inoculated with a 24 h seed culture of $Bifidobacterium$ Longum B6., $Lactobacillus$ $casei$., and incubated at $36^{\circ}C$ for 72 h. $Bifidobacterium$ Longum B6 was specifically was found to show the best growth on $3,255{\times}10^6\;CFU/m{\ell}$ after 48 h of fermentation. The content of ginsenoside Rb1, Re and Rd were decreased with the fermentation but ginsenoside Rh2 and Rg2 increased after fermentation process. In the case of low molecular ginsenoside conversion yields were 56.07% of Rh2, 12.03% of Rg3 and 77.11% of Rg2, respectively. In addition, compound-K was irregular conversion yield as long as 72 h of fermentation. This results indicate that fermentation process could increase the low molecular ginsenoside in low grade fresh ginseng.

• 농업과학연구
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• 제38권2호
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• pp.205-212
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• 2011

The potential factors for quality loss of cleaned fresh ginseng and technology to be associated with the improvement of marketability through pre-packaging fumigation were examined. Major microorganisms isolated from fresh ginseng included Botrytis cinerea, and Erwinia sp. Others such as Cylindrocarpon sp., Fusarium spp., Pennicilium spp., Bacillus spp. were also found at relatively low frequency. The bacterial density of vacuum packaged fresh ginseng rapidly increased during simulated marketing. Little correlation between bacterial growth and package swelling was found. In order to improve packaging method of fresh ginseng, pre-packaging treatment of 2-phenylethyl alcohol (PE, 100 uL/L, 4 hr) was examined. The fumigation treatment effectively inhibited the growth of bacteria density and also effective on keeping firmness of ginseng root, especially in cortical portion. The internal gas compositions of plastic container packaged for ginseng were approximately ranged between 6 to 8% $O_2$and 3 to 4% $CO_2$. The $O_2$ level of fumigation treatment was lower than control whereas $CO_2$ level was higher. The upsurge of ethylene evolution I day after simulated marketing was found only in fumigation treatment but it returned to ordinary level at day 2. The sucrose content of 2-PE treatment was significantly reduced at 5 days after simulated marketing but reducing sugars like glucose and fructose remained at higher level. The difference in sugar levels was reduced after 10 days of simulated marketing. The decay of fresh ginseng began at the lateral or fine root, which is weak to physical damage, in general. The epidermis was more damaged. Plastic container packaging with PE fumigation could be an alternative to vacuum packaging, which allows an aerobic environment and prevents anaerobic respiration. Further study of pre-package fumigation is required to improve technology of fresh ginseng marketing.

• 한국식품저장유통학회지
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• 제18권4호
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• pp.459-466
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• 2011

수삼의 적정 저장 온도 규명을 위하여 수삼을 $-3^{\circ}C$, $-1.5^{\circ}C$ 및 $0^{\circ}C$에 각각 저장하면서 저장 중 수삼의 품질 변화를 조사하였다. 수삼의 저장 중 변질율은 저장 8주까지 저장온도가 낮을수록 변질율이 낮았으나 이후부터는 오히려 $-3^{\circ}C$에서 저장한 수삼의 변질율이 급격히 증가하였다. 수삼의 변질된 면적을 기준으로 산출한 변질정도는 전반적으로 $0^{\circ}C$에서 저장한 수삼이 가장 심하였고, 다음은 $-1.5^{\circ}C$에서 저장한 수삼이었으며, $-3^{\circ}C$에서 저장한 수삼이 가장 낮았다. 중량 감소율은 $-1.5^{\circ}C$에서 저장한 수삼이 가장 낮았고, $-3^{\circ}C$와 $0^{\circ}C$에서 저장한 수삼은 유사한 수준이었다. 미생물 수는 저장 12주까지는 저장온도가 낮을수록 적은 것으로 나타났으나 저장 14주 이후에는 $-3^{\circ}C$에서 저장한 수삼의 미생물 수가 급격히 증가되어 총균 수는 $0^{\circ}C$와 $-1.5^{\circ}C$에서 저장한 수삼과 유사한 수준이었고, 곰팡이 수는 오히려 높은 것으로 나타났다. 표면색도는 $-3^{\circ}C$에서 저장한 수삼이 $0^{\circ}C$와 $-1.5^{\circ}C$에서 저장한 수삼에 비해 비교적 변색이 빠르게 진행되는 것으로 나타났다. 수삼 뇌두부위의 전해질 용출도는 저장기간이 경과함에 따라 증가하였는데 $-3^{\circ}C$에서 저장한 수삼이 $-1.5^{\circ}C$ 및 $0^{\circ}C$에서 저장한 수삼보다 높았다. 수삼의 관능적 품질은 저장 4주 후까지 저장온도에 따른 차이를 나타내지 않았고, 수삼 뇌두부위의 품질이 3.0이하로 평가된 시점은 $-3^{\circ}C$에서는 저장 8주 후, $-1.5^{\circ}C$에서는 12주 후, $0^{\circ}C$에서는 14주 후 이었고, 종합적인 관능적 품질이 3.0이하로 평가된 시점은 $-3^{\circ}C$에서는 저장 10주 후, $-1.5^{\circ}C$ 및 $0^{\circ}C$에서는 저장 14주 후이었다.