• Korean Journal of Acupuncture
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• v.30 no.4
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• pp.272-280
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• 2013

Objectives : The aim of this study was to observe effects of manual acupuncture(MA), electro-acupuncture(EA) and moxibustion (MO) on the rat with loperamide-induced constipation. Methods : This study measured small intestinal motility and number of fecal pellets in rats with loperamide induced constipation. MA and EA(intensity, 5 times muscle twitch threshold) was applied for 30 minutes and MO was treated on 5 times moxa cautery to the groups divided with age and sex. Results : The small intestinal motility was decreased by ST36 EA and ST37 EA in 5 weeks male group and ST37 EA in 7 weeks male and female groups, and ST36 MO in 7 weeks female group, but it was increased by ST39 EA in 7 weeks male group and ST37 MO and ST39 MO in 7 weeks male group. The number of fecal pellets was decreased by MA, EA, MO of ST36 in all group except 5, 7 weeks male groups only, and MA, EA of ST37 in 5, 7 weeks male group and ST37 MO in 7 weeks female group, and ST39 EA in 5 weeks male group and 7 weeks female group and ST39 MO in 7 weeks female group, but increased by ST37 EA in 5 weeks female group and ST39 EA in 7 weeks male group. Conclusions : Although these differences do not have a established tendency, it suggests that acupuncture and moxibustion are experimentally dependent upon the sex and age on intestinal motility in rats.

• Korean Journal of Medicinal Crop Science
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• v.24 no.6
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• pp.471-478
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• 2016

Background: Constipation is one of the most common functional gastrointestinal disorder. The present study examined the ability of water extract of fermented (FRC) and non-fermented (NFRC) roasted Cassia tora to improve intestinal function and reduce constipation in a rat constipation model. Methods and Results: Different concentration of FRC and NFRC were orally administered loperamide (5 mg/kg; LOP) reduced the number, weight, and water content of feces, as well as intestinal transit motility. However, 24 h-(24 hour fermented roasted-Cassia tora) 300 mg/kg FRC administration increased the number, weight, and water concent of feces, compared to that seen in the LOP group, and also improve intestinal transit mitility and, the thickness of distal colon and mucous fluid. Conclusions: The results of the present study indicated that LOP-induced constipation was improved by treatment with FRC. Therefore FRC could be used to develop functional foods or natural medicine for constipation. However, further study is needed to clarify how fermentation improves the medicinal properties of roasted C. tora.

• Journal of Acupuncture Research
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• v.25 no.4
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• pp.59-69
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• 2008

Objectives : The purpose of this study was to observe the effects of acupuncture and electro-acupuncture(EA) at $LI_{11}$ on intestinal motility in rats. Methods : We made over-activated and suppressed state of intestinal motility with carbachol and loperamide in rat and carried out acupuncture with needle retained(NR), low frequency(2 Hz) EA and high frequency(100Hz) EA at $LI_{11}$ before or after the administration of carbachol or loperamide. The charcoal travel rate was measured to evaluate the intestinal motility. Results : 1. NR, 2Hz EA and 100Hz EA at $LI_{11}$ showed no significant influences on intestinal motility of rat in normal state. 2. All of the pre-treatment and post-treatment of NR, 2Hz EA and 100Hz EA at $LI_{11}$ showed no significant effects than control group on intestinal motility of rat which was over-activated with carbachol. 3. The pre-treatment of 2Hz EA and 100Hz EA at $LI_{11}$ on intestinal motility of rat which was suppressed with loperamide showed no significant influences but in the L-$LI_{11}$-EA(L) and L-$LI_{11}$-EA(H) groups, intestinal motility was significantly increased than L-control group. Conclusions : These results suggest that acupuncture and EA on $LI_{11}$ have preventive effect and therapeutic effect on suppressed intestinal motility, and EA is more effective than NR.

• Journal of Acupuncture Research
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• v.25 no.4
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• pp.11-29
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• 2008

Objectives : The purpose of this study was to observe the effects of acupuncture and electro-acupuncture at $LI_4$ on intestinal motility in rat. Methods : We made over-activated state of intestinal motility with carbachol and suppressed state of intestinal motility with loperamide in rat and carried out needle retention acupuncture, low frequency electro-acupuncture and high frequency electro-acupuncture at $LI_4$ or sham point in rat devided into pre-treatment group and post-treatment group. and we resulted as follow for measuring charcoal travel rate to observe intestinal motility. Results : 1. Any of needle retention acupuncture, low frequency electro-acupuncture and high frequency electro-acupuncture at $LI_4$ have no significant influences on intestinal motility of rat in normal state. 2. All of the pre-treatment and post-treatment of needle retention acupuncture, low frequency electro-acupuncture and high frequency electro-acupuncture didn't significantly decrease intestinal motility in rat which over-activated with carbachol. 3. Pre-treatment of needle retention acupuncture and high frequency electro-acupuncture significantly increased intestinal motility in rat which suppressed with loperamide. 4. Post-treatment of needle retention acupuncture, low frequency electro-acupuncture and high frequency electro-acupuncture significantly increased intestinal motility in rat which suppressed with loperamide. Conclusions : These results suggest that acupuncture on $LI_4$ have preventive effect and therapeutic effect on suppressed intestinal motility, and especially high frequency electro-acupuncture is more effective.

• Journal of Applied Biological Chemistry
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• v.63 no.4
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• pp.365-373
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• 2020

We previously reported the potential of Senna tora L. seeds fermented by Lactobacillus casei (FSL) as a laxative agent in a loperamide-induced constipation rat model. Here, we examine the mechanism of action of FSL and its bioactive compound, revealed herein, on loperamide-induced constipation Sprague Dawley rat model. We identified the compound aurantio-obtusin (AO) using HPLC quantitative analysis. Rats were randomly assigned to six experimental groups (eight rats each)-normal and constipated groups (loperamide, FSL [100, 300, 500 mg/kg], and AO [1 mg/kg]). The FSL and AO-treated group showed an increase in the frequency, amount, and water content of feces in the constipated rat. Moreover, FSL and AO increased the intestinal transit speed in the constipated rat. Histological analysis revealed that FSL and AO recovered the intestinal mucus, the number of goblet cells, as well as thickness of the mucosa layer and muscle. Furthermore, the protein levels of the muscarinic acetylcholine receptor M3, which is involved in intestine contraction, were recovered in the FSL and AO-treated group. Its downstream signaling pathway (p-protein kinase C) was recovered by FSL and AO treatment. In conclusion, fermentation of S. tora L. seeds increases AO, which improves intestinal function, indicating that FSL is effective for treating constipation.

• Korean journal of food and cookery science
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• v.29 no.2
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• pp.185-192
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• 2013

The present study was conducted to evaluate the effects of Agarum cribrosum on weight change and defecation states in rats with constipation induced by loperamide. Food intake and body weight both decreased in the 5% Agarum cribrosum and loperamide-treated group (SD5) and 10% Agarum cribrosum and loperamide-treated group (SD10), whereas fecal water contents increased by 1.6 and 2.1-fold in the SD5 and SD10 groups, respectively. The concentrations of total-cholesterol, HDL-cholesterol and triglyceride in the sera of the SD5 and SD10 groups were lower than those in the control (C) group. However, the biochemical parameters, GOT (glutamic oxaloacetic transaminase), GPT (glutamic pyruvic transaminase), and glucose levels, were not affected by the level of Agarum cribrosum. In addition, the concentrations of total-cholesterol and triglyceride in the livers of the SD5 and SD10 groups were also lower than those in the control group. The results of the present study demonstrated that Agarum cribrosum might ameliorate constipation symptoms, and lower lipid concentrations in the blood and liver.

• Journal of Acupuncture Research
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• v.25 no.5
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• pp.27-42
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• 2008

Objectives : The purpose of this study was to observe the effects of acupuncture and electro-acupuncture at $LR_3$ on intestinal motility in rat. Methods : We made over-activated state of intestinal motility with carbachol and suppressed state of intestinal motility with loperamide in rat and carried out needle retention acupuncture and high frequency electro-acupuncture at $LR_3$ in rat devided into pre-treatment group and post-treatment group. The charcoal travel rate was measured to evaluate the intestinal motility. Results : 1. High frequency electro-acupuncture at $LR_3$ have significant influences on intestinal motility of rat in normal state. 2. Pre-treatment of needle retention acupuncture, high frequency electro-acupuncture significantly decreased intestinal motility in rat which over-activated with carbachol. 3. Post-treatment of high frequency electro-acupuncture significantly increased intestinal motility in rat which suppressed with loperamide. Conclusions : These results suggest that acupuncture and electro-acupuncture at $LR_3$ are effective on intestinal motility in rat.

• Journal of Acupuncture Research
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• v.26 no.2
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• pp.79-89
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• 2009

Objectives : The aim of this study was to observe the effect of acupuncture and high frequency electroacupuncture at $ST_{39}$ on intestinal motility in rats. Methods: We made over-activated state of intestinal motility with carbachol and suppressed state of intestinal motility with loperamide in rat and carried out needle retention acupuncture and high frequency electroacupuncture at $ST_{39}$ or sham point in rat divided into pre-treatment group and post-treatment group. We fed charcoal to them after the treatment and measured the travel rate of charcoal in the gastrointestinal tract to observe intestinal motility. Results : Examining intestinal motility of normal rat treated with needle retention acupuncture at $ST_{39}$ significantly suppressed intestinal motility. Pre-treatment of needle retention acupuncture significantly suppressed intestinal motility in rat which over-activated with carbachol. Pre-treatment and post-treatment of high frequency electroacupuncture significantly suppressed intestinal motility in rat which over-activated with carbachol. All of the pre-treatment and post-treatment of needle retention acupuncture, high frequency electroacupuncture showed no significant effect than control group on intestinal motility of rat which was suppressed with loperamide. Conclusions : These results suggest that acupuncture and high frequency electroacupuncture at $ST_{39}$ have preventive and therapeutic effects on over-activated intestinal motility, and high frequency electroacupuncture is more effective.

• Korean Journal of Food Science and Technology
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• v.36 no.6
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• pp.991-994
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• 2004

Effects of formulation KTG075 from edible plants on intestinal function, particularly on Mucin 2 secretion, were examined by loperamide-induced constipation method using Sprague Dawley rats (SD rats, male). Crypt epithelial cells containing more mucus and mucus layer stained with alcian blue were significantly thicker in KTG075 group than control group. When Biogenex AM358 of antibody against Mucin 2 was used, crypt epithelial cells secreted more Mucin 2 in KTG075 group than control group. The Mucus layer at fecal surface was thinner and less mucus was recovered from mucosal surface in constipated rats than in KTG075 group. Mucus production of crypt epithelial cells and mucus contents at fecal and mucosal surfaces were reduced by loperamide-induced constipation. These results indicate formula KTG075 accelerates evacuation and activates intestines.

• Korean Journal of Food Science and Technology
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• v.40 no.3
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• pp.326-331
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• 2008

This study investigated the effects of dietary slander glasswort extract on improvements in bowel function and constipation relief. Balb/C mice were used to measure transit movement of a charcoal meal after slander glasswort extract was added to their drinking water at 0.36, 0.72, and 1.5 mg/mL concentrations. The extract's constipation relief effects were investigated in Sprague-Dawley rats with loperamide-induced constipation by adding 2, 3, and 4 mg/mL of the extract to their drinking water, and by feeding yogurt containing 0.0, 0.25, and 0.50% slander glasswort extract mixed into a 10-fold amount of water. The mice receiving the extract at 1.5 mg/mL via drinking water had a 16.4% increase in the rate of transit as compared to the controls. In addition, the rats receiving the extract via drinking water and yogurt had increased in constipation relief from 128 to 156% and from 107 to 150%, respectively, as compared to the controls. Based on the results, slander glasswort is considered to have effects on improving bowel function and relieving constipation.