• 아시안잔디학회지
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• 제25권1호
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• pp.52-58
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• 2011

난지형 잔디에 사용되는 덧 파종 초종으로는 퍼레니얼 라이그래스가 대표적으로 이용되어 있다. 그러나 덧 파종된 퍼레니얼 라이그래스는 여름철이 되어도 기존잔디와 경합으로 살아남는 개체가 많아 잔디 표면의 규일성을 떨어뜨리는 경우가 많으므로 인위적인 제거가 필요하다. 한국잔디는 Kerb에 의한 영향을 거의 받지 않은 반면에 퍼레니얼 라이그래스는 Kerb처리에 의하여 생육감소가 많았고 고사한 신초의 비율이 많이 높아지는 것으로 보아 퍼레니얼 라이그래스를 제거하기 위해서는 Kerb를 처리함이 효과적인 것으로 판단된다. 차광은 정도에 따라 영향은 다르지만 퍼레나얼 라이그래스의 생육을 향상시키고 Kerb에 의한 영향을 감소시켰다. 차광률이 75% 정도 되는 그늘에서는 Kerb 처리량이 $0.4\;g{\cdot}m^{-2}$이상이 되어야 퍼레니얼 라이그래스에서 고사되는 신초의 비율이 높고 생육이 감소되는 것으로 나타났다. 따라서 햇빛의 투과량이 적은 그늘 조건하에서는 Kerb의 처리농도가 기준량 보다 더 높아져야 퍼레니얼 라이그래스를 효과적으로 제거할 수 있을 것으로 예측된다.

• 아시안잔디학회지
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• 제15권4호
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• pp.169-179
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• 2001

근권부의 온도를 대조구에 비하여 낮게 조절하고 토양조성을 달리하여 여름철 고온기에 Kentucky bluegrass (Poa pratensis L.‘Nuglade’), per-ennial ryegrass(Lolium perenne L.‘Accent’), tall fescue (Festuca arundinacea Schreb. ‘Pixie’), Japanese lawngrass (Zoysia japonica Steud.)를 재배하고 잔디의 품질, 병해발생 정도 엽록소 함량을 조사하고 아울러 예지물의 무기물 함량을 측정하여 다음과 같은 결과를 얻었다. 1. 여름철 근권부의 온도를 4~6$^{\circ}C$ 낮게 처리한 경우 한지형 잔디의 균일성을 현저히 높여주고 병의 발생률을 크게 억제시켰으며 그 효과는 perennial ryegrass에서 가장 큰 것으로 나타났다. 엽록소 함량은 토양조성에 따라 변화가 없었으나 냉온 처리구에서 대조구에 비하여 크게 증가되었다. 2. 여름철 근권부의 냉온처리는 Kentucky blue-grass와 perennial ryegrass는 대조구에 비하여 약 2배, tall fescue는 2.5배 이상의 clipping무게 증가를 나타냈다. 난지형 잔디인 들잔디에서도 냉온처리구에서 생육의 증진효과를 보였다. 한지형 잔디는 모래 ＋peat moss를 80 : 20(v/v)으로 혼합한 토양에 비하여 모래＋peat moss＋일반토양을 80：10 : 10 (v/v/v)으로 혼합한 토양에서 많은 생장을 보였다. 3. 근권부의 냉온처리는 공시된 세 종류의 한지형 잔디에서 N, P, K, Ca그리고 Mg의 흡수를 증가시켰으나 들잔디에서는 흡수차이를 보이지 않았다. 토양의 혼합비율에 따른 무기물함량의 차이는 인정되지 않았다. 이상의 결과로 미루어 근권부의 냉온처리는 고온 스트레스에 의한 잔디의 생육저하를 감소시키고 여름철 하고현상을 예방하여 줌으로써 고온다습한 기후조건에서도 경기장의 잔디 품질을 우수하게 유지할 수 있는 방안으로 판단된다.

• Animal Bioscience
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• 제34권12호
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• pp.2023-2033
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• 2021

Objective: The present study was conducted to assess the effect of urease inhibitor (hydroquinone [HQ]) and nitrification inhibitor (dicyandiamide [DCD]) on nitrogen (N) use efficiency of pig slurry for perennial ryegrass regrowth yield and its environmental impacts. Methods: A micro-plot experiment was conducted using pig slurry-urea ¹⁵N treated with HQ and/or DCD and applied at a rate of 200 kg N/ha. The flows of N derived from the pig slurry urea to herbage regrowth and soils as well as soil N mineralization were estimated by tracing pig slurry-urea ¹⁵N, and the N losses via ammonia (NH₃), nitrous oxide (N₂O) emission, and nitrate (NO₃^-) leaching were quantified for a 56 d regrowth of perennial ryegrass (Lolium perenne) sward. Results: Herbage dry matter at the final regrowth at 56 d was significantly higher in the HQ and/or DCD applied plots, with a 24.5% to 42.2% increase in ¹⁵N recovery by herbage compared with the control. Significant increases in soil ¹⁵N recovery were also observed in the plots applied with the inhibitors, accompanied by the increased N content converted to soil inorganic N (NH₄⁺+NO₃^-) (17.3% to 28.8% higher than that of the control). The estimated loss, which was not accounted for in the herbage-soil system, was lower in the plots applied with the inhibitors (25.6% on average) than that of control (38.0%). Positive effects of urease and/or nitrification inhibitors on reducing N losses to the environment were observed at the final regrowth (56 d), at which cumulative NH₃ emission was reduced by 26.8% (on average 3 inhibitor treatments), N₂O emission by 50.2% and NO₃^- leaching by 10.6% compared to those of the control. Conclusion: The proper application of urease and nitrification inhibitors would be an efficient strategy to improve the N use efficiency of pig slurry while mitigating hazardous environmental impacts.

• 생물환경조절학회지
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• 제32권3호
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• pp.242-248
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• 2023

본 연구는 퍼레니얼 라이그래스에서 생장조정제 trienexapacethyl과 prohexadione-calcium을 처리하였을 때 생육 시기별 잔디 품질과 생육 억제 효과를 조사하였다. 처리구는 대조구(Control), TE 처리구(0.01 a.i. g·m^-2·100mL^-1) 및 PC 처리구(0.01 a.i. g·m^-2·100mL^-1)으로 설정하였다. 생장조정제 처리 후 퍼레니얼 라이그래스의 엽색 지수 및 엽록소 함량은 대조구와 통계적으로 유의적인 차이를 나타내지 않았다. TE와 PC처리구에서 잔디의 초장은 각각 8.9-12.4%와 6.7-13.6%씩 감소하였고, 잔디 예지물량은 44.4-45.8%와 40.6-40.9%씩 감소하였다. 생장조정제 처리 후 경과 일수와 초장의 변화를 이용하여 약효 지속 기간을 조사하였을 때, TE와PC처리구는 1차 조사(7월 29일-8월 26일)에서 각각 43.6일과 37.9일, 2차 조사(9월 30일-10월 28일)에서는 38.3일과 39.5일이었다. 상기 결과들을 종합할 때, TE와 PC의 처리는 퍼레니얼 라이그래스의 초장 및 생육을 억제하였고, 약효 지속 기간은 약 40 ±3일 정도를 나타냈다. 또한 생장조정제 처리시 계절별 잔디 생육과 품질에 영향을 미치지 않았으며, 약해는 발생하지 않았다.

• 아시안잔디학회지
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• 제13권2호
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• pp.71-78
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• 1999

The influence of increasing livel (0.0, 0.05, 0.1, 0.2, 0.4, 0.6, 0.8, 1.2, 1.6, and 2.0%) fo NaCl on the germination of red fescue (Festuca rubra) 'Sea Breeze', tall fesce(Festucaarundinacea) 'Pixie', creeping red fescue (Festuca rubra ssp. rubra) 'Cindy', annual rye-grass (Lolium multiflorum) 'Permer Ⅱ', perennial ryegrass (Lolium perenne) 'Pennant', fairway wheatgrass(Agropyron cristatum), creeping bentgrass (Agrostis palustris) 'Penncross', and kentucky bluegrass (Poa pratensis) 'Nuglade' was investigated. Red fescue 'Sea Breeze', tall fescue 'Pixie', and creeping red fescue 'Cindy' had greater than 90% seed germination at NaCl concentrations of 0.2% or lower, while showing similar seeding shoot and root lengths and TAA50 values as the control. Creeping red fescue 'Cindy'gave at 0.6% or higher NaCl. Perennial ryegrass 'Pennant' and annual ryegrass 'Permer Ⅱ' showed more than 95% seed germination when NaCl concentrations were 0.4% and 0.8%, respectively. Fairway wheatgrass, creeping bentgrass 'Penncross' and Kentucky bluegrass 'Nugade' had showing similar germination percent, shoot and root lengths and T50 values as the control at NaCl concentrations of 0.1% or lower. In general, germination percent and the lengthes of seedling roots and shoots of all species tested decreased as NaCl concentrations iscreased. The T50 values became greater as NaCl concentration increased. Seed fermination in red fescue 'Sea Breeze', tall fescue 'Pixie',perennial ryegrass 'Pennant', and annual ryegrass 'Permer Ⅱ' was compoetely inhibited at 2.0% NaCl. Creeping red fescue 'Cindy' and fairway wheatgrass gardly germinated at 1.6% MaCl. Creeping bentgrass 'Penncross' and Dentucky bluegrass 'Nuglade' showed a complete inhibition of germination at 1.2% and 0.6% NaCl, respectively.

• 한국환경복원기술학회지
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• 제8권3호
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• pp.13-20
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• 2005

This study was conducted to suggest the ecological restoration methods of the decomposed granite cut-slope by organic soil amendment materials. Field test carried out for the cut-slope with organic soil amendment materials method and other three revegetation methods in Cheongwon. Test revegetation plants were Festuca arundinacea, Lolium perenne, Dactylis glomerata, Lespedeza cyrtobotrya, and Arundinella hirta. The result of this study can be summarized as follows; 1. The soil hardness, the soil acidity, and the soil humidity of organic soil amendment materials method were at a suitable value for plants growth. And it was better as compared with other three revegetation methods of cutting-rock slopes. 2. The result of toxic substance investigation, all items were at a suitable for standard law. 3. During one year after seeding, most plants germinated and especially Festuca arundinacea and Dactylis glomerata grows well. Seedling numbers were 336.7 per $m^2$(after 6 months), 183.3 per $m^2$(after 10 months), and 353.3 per $m^2$(after 6 months). Ten months later after seeding, plants showed 80% ground coverage. Visual rate, plant height, and growth rate were excellence, Also, high plant growth in spring better than autumn.

• Asian-Australasian Journal of Animal Sciences
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• 제4권3호
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• pp.245-250
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• 1991

Analysis of the characteristics and the grouping of the species of sown and weeds in artificial pastures was studied applying the principal component analysis method. Presency and coverage of six sown species and fifteen weed species which occurred in pastures of under-grazing and optimumgrazing were subject to analysis. From field survey, species were divided into three groups: the group A included five species such as Festuca arundinacea, Lolium perenne and Dactylis glomerata, etc., the group B included eleven species such as Polygonum longisetum, Agrostis alba and Rumex obtusifolius, etc., and the group C included five species such as Miscanthus sinensis, Rubus palmatus and Artemisia princeps, etc. The group A species corresponded to good pasture conditions and management. On the contrary, the group C species occurred in poor pasture conditions with inadequate management. The group B species corresponded to intermediate pasture conditions and management. Interrelated pair species co-existing and species non-co-existing were discovered. Factor loading as negative for the group A species. positive for the group C species and positive but lower than the group C species for the group B species. From these results it is concluded that the principal component analysis seems to one of the useful tools for the analysis of characteristics of species and the diagnosis of sown pasture vegetation, although further studies are required to get more general information about species characteristics.

• Plant Biotechnology Reports
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• 제2권3호
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• pp.171-177
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• 2008

This paper reports an improved protocol for isolation, culture and regeneration of Lotus corniculatus protoplasts. A range of parameters which influence the isolation of L. corniculatus protoplasts were investigated, i.e., enzyme combination, tissue type, incubation period and osmolarity level. Of three enzyme combinations tested, the highest yield of viable protoplasts was achieved with the combination of 2% Cellulase Onozuka RS, 1% Macerozyme R-10, 0.5% Driselase and 0.2% Pectolyase. The use of etiolated cotyledon tissue as a source for protoplast isolation proved vital in obtaining substantially higher protoplast yields than previously reported. Culture of the protoplasts on a nitrocellulose membrane with a Lolium perenne feeder-layer on the sequential series of PEL medium was highly successful in the formation of microcolonies with plating efficiencies 3-10 times greater than previous studies. Shoot regeneration and intact plants were achieved from 46% of protoplast-derived cell colonies.

• 한국환경복원기술학회지
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• 제9권6호
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• pp.38-51
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• 2006

This study was carried out to improve rehabilitation effect by hydro-seeding methods on denuded slopes. Lespedeza cyrtobotrya, Indigofera pseudotinctoria, Arudinella hirta, Poa pratensis, and Lolium perenne were used in this study. The results of the germination and growth characteristics by seed coating, slope control and soil mulching treatments are summarized here. Seed coating of those plants had an effect on germination ratio, germination force, $LD_{50}$, and seedling growth of stem, leaf and root. The seed coating effect was highest for I. pseudotinctoria while the seedling growth was best for L. cyrtobotrya. Vermiculite+Talcum was the best coating material for germination and seedling growth of the plants. As the slopes were steeper, germination ratio was lower and seedling growth of stem and root decreased. Lots of seeds and soils were swept away when the slope was steep. Soil mulching was effective for germination, seedling growth of stem and root, and soil stabilization. It was more effective when the slope was steeper. Coir net was the most effective soil mulching material n this study.

• 한국초지조사료학회지
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• 제20권2호
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• pp.77-84
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• 2000

Eight-week old perennial ryegrass (Lolium perenne L. cv. Reveille) plants were exposed to different NO3-concentrations or osmotic stress with NaCI. Previously labeled "N was chased during 14 days of non-labeled'NO3 feeding in order to investigate NO3 metabolism in relation to osmoregulation. The short termmeasurement of osmotic potential showed that the extemal concentration of Nos- had not great effect on theosmotic potential, but that osmotic adjustment was observed in NaCl-treated plants. Total uptake of NO 3 - waslargely increased by increasing supply level of NO3 while it was depressed by exposing to osmotic stress.Nitrate reduction increased to more than 29% by increasing extemal NO,- concentration from 1 mM to 10mM. When osmotically stressed with NaCI, nitrate reduction was depressed to about 37% as compared to thecontrol. The decrease in translocation of reduced N into leaves was also observed in NaCl exposed plants. Inthe medium exposed to 10 mM NO,., osmotic contribution of nitrate to cumulative osmotic potential wasdecreased, and it was osmotically compensated with soluble carbohydrate. When osmotically stressed withNaC1, the contribution of chloride was much higher than that of nitrate. The present data indicate that N03-in plant tissues, factually affected by the assimilation of this ion, plays an active role in osmotic regulation incorrelation with other osmotica such carbohydrate and chloride.(Key words : Nitrate metabolism, Osmotic stress, Nitrate supply level, Osmoregulation)ate supply level, Osmoregulation)